Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,336,983 G→T 6.6% L17M (CTG→ATG) ‡ adiC ← arginine:agmatine antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,336,9830GT 6.6% 285.9 / 5.9 97L17M (CTG→ATG) ‡adiCarginine:agmatine antiporter
Reads supporting (aligned to +/- strand):  ref base G (43/47);  new base T (3/4);  total (46/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01

GGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGACCGGGTTATCGCTGAT  >  NC_000913/4336836‑4337131
                                                                                                                                                   |                                                                                                                                                    
ggtaCGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAggg                                                                                                                                                    <  1:1156340/147‑1 (MQ=255)
ggAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAggg                                                                                                                                                    <  1:491217/150‑1 (MQ=255)
 gAACGACATTTTGGCGTATACCCTCGAGAGCCCCAGTGCACCGATTATCGTCCCCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTAGCAGGTAACAGACCGGCCCCGGCCCCCATAATATTCCCCGCCCCCATCAgggg                                                                                                                                                   >  2:305483/1‑149 (MQ=255)
   aCGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCAAGGTTTGCATGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACCCCATCAGGGTGa                                                                                                                                                 >  2:359147/1‑150 (MQ=255)
    cGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGAc                                                                                                                                                >  1:361589/1‑150 (MQ=255)
      aCATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGTCACCCTGATGGTGTCgg                                                                                                                                              <  1:237593/150‑1 (MQ=255)
      aCATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCACCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGTCACCCTGATGGTGTCgg                                                                                                                                              <  2:377306/150‑1 (MQ=255)
       cATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCAATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGCCCCCATAATATTCCCCGACACCATCAGGGTGACggg                                                                                                                                             >  1:119210/1‑150 (MQ=255)
        aTTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGTCACCCTGATGGTGTCgggg                                                                                                                                            <  1:1344045/150‑1 (MQ=255)
        aTTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACgggg                                                                                                                                            <  2:821493/150‑1 (MQ=255)
         ttttGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGTCACCCTGATGGTGTCGGGGa                                                                                                                                           <  1:397816/150‑1 (MQ=255)
         ttttGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGa                                                                                                                                           <  1:540114/150‑1 (MQ=255)
          tttGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGAt                                                                                                                                          <  1:1426253/150‑1 (MQ=255)
              gCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCCCCAACCATCCATAAATGGCAATCCCCCCAGTACACGCCAGGTTCGCAGGTAGCAGAAAAACACCAGACCCCAAAATATTCCCCGACACCATCAGGGGGACGGGGATTAAg                                                                                                                                      >  2:1412149/1‑150 (MQ=255)
              gCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAg                                                                                                                                      <  2:471799/150‑1 (MQ=255)
               cGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTATCAGAAAAACACCTGACCCCAGAATATTCCCCGACACCATCAGGGTGACGGGGTTTAAGc                                                                                                                                     >  1:1723039/1‑150 (MQ=255)
                    aCCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACt                                                                                                                                <  1:1386926/150‑1 (MQ=255)
                    aCCATCCAGAGCCCCAGTGCACCGAAAATCGTCACCAACCATCCATAAATCGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACt                                                                                                                                <  1:305483/150‑1 (MQ=255)
                     ccATCGAGAGCCCCAGTGCACCGATAATCGTCCCCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACtt                                                                                                                               <  1:1610863/150‑1 (MQ=255)
                     ccATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACtt                                                                                                                               >  1:1808867/1‑150 (MQ=255)
                     ccATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACtt                                                                                                                               >  2:452293/1‑150 (MQ=255)
                        tCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTtgt                                                                                                                            <  2:1357967/150‑1 (MQ=255)
                         cGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTtgtg                                                                                                                           <  2:1786291/150‑1 (MQ=255)
                          gagaGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCTCCAGTAGCGGCCAGGTTTGCAGGTAACAGAACAACACCGGACCCCATAATATTCCCCGCCAACATAAGGTTGACGGGGATTAAGCCCACGTtgtgc                                                                                                                          >  2:121671/1‑149 (MQ=255)
                          gagaGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGa                                                                                                                          <  1:1641935/150‑1 (MQ=255)
                          gagaGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGa                                                                                                                          >  1:432413/1‑150 (MQ=255)
                          gagaGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGa                                                                                                                          <  2:822660/150‑1 (MQ=255)
                           agagCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGag                                                                                                                         <  2:1375679/150‑1 (MQ=255)
                               ccccAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGagcatc                                                                                                                     <  1:1365093/150‑1 (MQ=255)
                               ccccAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGagcatc                                                                                                                     >  2:1568372/1‑150 (MQ=255)
                                cccAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGagcatca                                                                                                                    >  2:1376047/1‑150 (MQ=255)
                                 ccAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCATGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACAACATCAGGGTGACGGGGATTAAGCCCACTTTGTGagcatcag                                                                                                                   <  2:944205/150‑1 (MQ=255)
                                 ccAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGagcatcag                                                                                                                   <  1:1478211/150‑1 (MQ=255)
                                 ccAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGagcatcag                                                                                                                   <  2:836411/150‑1 (MQ=255)
                                 ccAGTGCACCGATAATCGGCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGagcatcag                                                                                                                   <  1:1634672/150‑1 (MQ=255)
                                   aGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGagcatcagca                                                                                                                 <  2:945410/150‑1 (MQ=255)
                                       cACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCg                                                                                                             >  1:685002/1‑150 (MQ=255)
                                          cGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAg                                                                                                          <  2:1375400/150‑1 (MQ=255)
                                            aTAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGAc                                                                                                        <  2:642825/150‑1 (MQ=255)
                                                 cGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACCCCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCCCTGTGTGAGCATCAGCATCCGAAGACATAAt                                                                                                   >  1:1216020/1‑150 (MQ=255)
                                                 cGTCACCAACCATCCATAAATGGCAATCCCGCAAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACTCCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAAt                                                                                                   >  2:375484/1‑150 (MQ=255)
                                                  gTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAACACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACTGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAAtt                                                                                                  >  2:1453187/1‑150 (MQ=255)
                                                  gTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAAtt                                                                                                  >  1:1522620/1‑150 (MQ=255)
                                                  gTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAAtt                                                                                                  >  1:1515517/1‑150 (MQ=255)
                                                   tCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTa                                                                                                 <  2:685851/150‑1 (MQ=255)
                                                   tCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTa                                                                                                 >  1:106246/1‑150 (MQ=255)
                                                      ccaaccaTCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAACCACCTGCCCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCAGCCGAAGACATAATAAAAc                                                                                              >  1:1165810/1‑150 (MQ=255)
                                                      ccaaccaTCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAAc                                                                                              <  1:1089902/150‑1 (MQ=255)
                                                        aaccaTCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTc                                                                                            <  2:1768904/150‑1 (MQ=255)
                                                              ccatAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCTACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGa                                                                                      <  1:407745/150‑1 (MQ=255)
                                                                atAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAg                                                                                    <  2:1037660/150‑1 (MQ=255)
                                                                 tAAATTGCACTCCCGCCAGTAGAGGCCAGTTTTGCAGGTAACAGAAAAACACCTGACCCCATAATAATCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAgg                                                                                   <  1:344525/150‑1 (MQ=255)
                                                                 tAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAgg                                                                                   >  1:1166082/1‑150 (MQ=255)
                                                                   aaTGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCg                                                                                 <  1:617673/150‑1 (MQ=255)
                                                                   aaTGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGAATCCGAAGACATAATTAAACTCCTGCGAAGTCg                                                                                 <  2:931134/150‑1 (MQ=255)
                                                                    aTGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGa                                                                                >  1:1611813/1‑150 (MQ=255)
                                                                    aTGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGa                                                                                <  1:585485/150‑1 (MQ=255)
                                                                         aaTCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTc                                                                           >  1:411982/1‑150 (MQ=255)
                                                                          aTCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCg                                                                          >  1:1703514/1‑150 (MQ=255)
                                                                           tCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGGATCCGAAGACATAATTAACCTCATGCGAAGGCGAGcggcgt                                                                         >  2:297703/1‑145 (MQ=255)
                                                                              cGCCAGTAGAGGCCAGGTTTGCAGGTAACACAAAATCCCCTGACCCCATAATATTCCCCGACACCATCATGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGAc                                                                      >  2:757428/1‑150 (MQ=255)
                                                                               gCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACa                                                                     <  1:790884/150‑1 (MQ=255)
                                                                               gCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACa                                                                     <  1:550157/150‑1 (MQ=255)
                                                                                  aGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATa                                                                  >  2:249220/1‑150 (MQ=255)
                                                                                      gagGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAACCTCCTGCGAAGGCGAGCTTCGTGACAATAAAtt                                                              >  1:1154685/1‑150 (MQ=255)
                                                                                      gagGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAAtt                                                              >  1:16199/1‑150 (MQ=255)
                                                                                        ggCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTAc                                                            >  2:1152724/1‑150 (MQ=255)
                                                                                           cAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGtc                                                         <  1:1634985/150‑1 (MQ=255)
                                                                                             ggTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGtcat                                                       <  1:547023/150‑1 (MQ=255)
                                                                                                 tGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGtcatatca                                                   <  2:1070771/150‑1 (MQ=255)
                                                                                                    aGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATAc                                                >  2:1538057/1‑150 (MQ=255)
                                                                                                       tAACAGAAAAACACCTGCCCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGcc                                             >  2:353575/1‑150 (MQ=255)
                                                                                                          cAGAAAATCACCTGACCCCATTATATTCACCGTCACAATCAGTTTGACGGGGATTAAGCCCACTTTTTTAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTga                                          <  1:1674300/150‑2 (MQ=255)
                                                                                                            gAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCAt                                        >  1:1766710/1‑150 (MQ=255)
                                                                                                            gAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCAt                                        >  2:189356/1‑150 (MQ=255)
                                                                                                              aaaaCACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTc                                      <  2:361589/150‑1 (MQ=255)
                                                                                                               aaaCACCTGACCCCATAATATTCCCCTACACCATCTGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACACTAAATTACGTCATATCATACGCCTGCATTCg                                     <  2:1110407/150‑1 (MQ=255)
                                                                                                               aaaCACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCCTCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCCTGACAATAAATTACGTCATATCATACGCCTGCAttag                                     >  1:889645/1‑148 (MQ=255)
                                                                                                               aaaCACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCg                                     >  2:1451803/1‑150 (MQ=255)
                                                                                                                 acacCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGAt                                   <  1:841006/150‑1 (MQ=255)
                                                                                                                 acacCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGAt                                   <  2:120121/150‑1 (MQ=255)
                                                                                                                  cacCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATa                                  >  2:612883/1‑150 (MQ=255)
                                                                                                                   acCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAg                                 >  1:5157/1‑150 (MQ=255)
                                                                                                                    ccTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGt                                >  1:1300401/1‑150 (MQ=255)
                                                                                                                     cTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGtt                               >  1:1645123/1‑150 (MQ=255)
                                                                                                                      tGACCCCATAATATTCCCAGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACCTAATTAAACTCCGGCGAAGGCGAGCTTCGTGACAATAAATTACGGCATATCATACGCCTGCATTCGATAGTTg                              <  1:121671/150‑1 (MQ=255)
                                                                                                                          cccATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAatt                          >  1:1279013/1‑150 (MQ=255)
                                                                                                                          cccATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAatt                          <  2:1808867/150‑1 (MQ=255)
                                                                                                                          cccATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAatt                          <  2:106246/150‑1 (MQ=255)
                                                                                                                          cccAAAATATTCCCCGACACCATCAGGGTGACTGGGATTAATCCCACATTGTGAGCCTCAGAATCCTAAGACATAAATAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAatt                          <  2:294218/150‑1 (MQ=255)
                                                                                                                           ccATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAatta                         >  2:1668045/1‑150 (MQ=255)
                                                                                                                           ccATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCGTCGTGACAAGAAATTACGTCATATCATACGCCTGCATTCGAGAGTTGAagta                         >  1:278388/1‑150 (MQ=255)
                                                                                                                            cATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGTCATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAattat                        <  2:390785/150‑1 (MQ=255)
                                                                                                                             ggcttaatCCCCGTCACCCTGATGGTGTCGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACAGAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAattatt                       >  1:291835/8‑150 (MQ=255)
                                                                                                                              taataTTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAAttatta                      >  2:804709/1‑150 (MQ=255)
                                                                                                                                 tatTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCAGTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACATTAAATTACGTCATAACATACTCATGCATTCGATAGTTGAATTATTATca                   >  1:1203918/1‑149 (MQ=255)
                                                                                                                                     ccccGTCACCCTGATGGTGTCGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGACCg               >  1:624058/1‑150 (MQ=255)
                                                                                                                                        ctacacCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCTACGACATAATTAAACTCCTGCGAAGGCGAGCTTCGGGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTTAATTATAATCGACCGGGt            <  1:1603966/148‑1 (MQ=255)
                                                                                                                                              cATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGACCGGGTTATCGc      <  1:1147095/150‑1 (MQ=255)
                                                                                                                                                  aGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGACCGGGTTATCGCTGAt  <  2:432413/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GGAACGACATTTTGGCGTATACCATCGAGAGCCCCAGTGCACCGATAATCGTCACCAACCATCCATAAATGGCAATCCCGCCAGTAGAGGCCAGGTTTGCAGGTAACAGAAAAACACCTGACCCCATAATATTCCCCGACACCATCAGGGTGACGGGGATTAAGCCCACTTTGTGAGCATCAGCATCCGAAGACATAATTAAACTCCTGCGAAGGCGAGCTTCGTGACAATAAATTACGTCATATCATACGCCTGCATTCGATAGTTGAATTATTATCGACCGGGTTATCGCTGAT  >  NC_000913/4336836‑4337131

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: