Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 492,230 C→A 5.5% R47S (CGT→AGT)  dnaX → DNA polymerase III/DNA elongation factor III, tau and gamma subunits

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913492,2300CA 5.5% 288.1 / 5.9 100R47S (CGT→AGT) dnaXDNA polymerase III/DNA elongation factor III, tau and gamma subunits
Reads supporting (aligned to +/- strand):  ref base C (50/44);  new base A (2/4);  total (52/48)
Fisher's exact test for biased strand distribution p-value = 4.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.29e-01

Only 100 of 102 total aligned reads displayed.

GAGCCTGCCAATGAGTTATCAGGTCTTAGCCCGAAAATGGCGCCCACAAACCTTTGCTGACGTCGTCGGCCAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTACCGCGACGCCGTGCGGCGTGTGCGATAACTGTCGTGAAATCGAGCAGGGGCGCTTTGTCGATCTGATTGAAATCGACGCCGC  >  NC_000913/492082‑492378
                                                                                                                                                    |                                                                                                                                                    
gagCCTGCCAATGAGTTATCAGGTCTTAGCCCGAAAATGGCGCCCACAAACCTTTGCTGACGTCGTCGGCCAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCg                                                                                                                                                     >  1:1732105/1‑150 (MQ=255)
 agCCTGCCAATGAGTTATCAGGTCTTAGCCCGAAAATGGCGCCCACAAACCTTTGCTGACGTCGTCGGCCAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGt                                                                                                                                                    <  2:1476358/150‑1 (MQ=255)
  gCCTGCCAATGAGTTATCAGGTCTTAGCCCGAAAATGGCGCCCACAAACCTTTGCTGACGTCGTCGGCCAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTg                                                                                                                                                   >  1:564465/1‑150 (MQ=255)
  gCCTGCCAATGAGTTATCAGGTCTTAGCCCGAAAATGGCGCCCACAAACCTTTGCTGACGTCGTCGGCCAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTg                                                                                                                                                   >  1:958038/1‑150 (MQ=255)
   ccTGCCAATGAGTTATCAGGTCTTAGCCCGAAAATGGCGCCCACAAACCTTTGCTGACGTCGTCGGCCAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTgg                                                                                                                                                  >  2:854767/1‑150 (MQ=255)
   ccTGCCAATGAGTTATCAGGTCTTAGCCCGAAAATGGCGCCCACAAACCTTTGCTGACGTCGTCGGCCAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTgg                                                                                                                                                  <  1:1448493/150‑1 (MQ=255)
    cTGCCAATGAGTTATCAGGTCTTAGCCCGAAAATGGCGCCCACAAACCTTTGCTGACGTCGTCGGCCAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTTGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGc                                                                                                                                                 >  2:1524151/1‑150 (MQ=255)
        cAATGAGTTATCAGGTCTTAGCCCGAAAATGGCGCCCACAAACCTTTGCTGACGTCGTCGGCCAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCg                                                                                                                                             <  1:585807/150‑1 (MQ=255)
         aaTGAGTTATCAGGTCTTAGCCCGAAAATGGCGCCCACAAACCTTTGCTGACGTCGTCGGCCAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCgg                                                                                                                                            <  1:1772773/150‑1 (MQ=255)
               ttATCAGGTCTTAGCCCGAAAATGGCGCCCACAAACCTTTGCTGACGTCGTCGGCCAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGACGCCACGGGTGCCGGaaaaaa                                                                                                                                      <  1:1268509/150‑2 (MQ=255)
                tATCAGGTCTTAGCCCGAAAATGGCGCCCACAAACCTTTGCTGACGTCGTCGGCCAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAAcc                                                                                                                                     <  1:1823384/150‑1 (MQ=255)
                tATCAGGTCTTAGCCCGAAAATGGCGCCCACAAACCTTTGCTGACGTCGTCGGCCAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGACGCCACGGGTGCCGGaaaaaag                                                                                                                                     <  2:724011/150‑3 (MQ=255)
                aaTCAGGTCTTAGCCCGAAACTCGCGCCCACAAACCTTTGCTGACGTCGTCGGCCAGGAACATGTGCGGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCATATCTTTTTTCCGACGCCACGGGTGCCGGaaaaaag                                                                                                                                     <  2:287491/149‑3 (MQ=255)
                 aTCAGGTCTTAGCCCGAAAATGGCGCCCACAAACCTTTGCTGACGTCGTCGGCCAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGACGCCACGGGTGCCGGaaaaaaga                                                                                                                                    <  1:1555255/150‑4 (MQ=255)
                    aGGTCTTAGCCCGAAAATGGCGCCCACAAACCTTTGCTGACGTCGTCGGCCAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGATAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAACACCTCTa                                                                                                                                 >  1:294142/1‑150 (MQ=255)
                     ggTCTTAGCCCGAAAATGGCGCCCACAAACCTTTGCTGACGTCGTCGGCCAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCCCCCGTGGCGTCGGAAAAACCTCTAt                                                                                                                                >  1:360364/1‑150 (MQ=255)
                       tCTTAGCCCGAAAATGGCGCCCACAAACCTTTGCTGACGTCGTCGGCCAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCg                                                                                                                              <  2:1409499/150‑1 (MQ=255)
                        cTTAGCCCGAAAATGGCGCCCACAAACCTTTGCTGACGTCGTCGGCCAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCAGTGGCGTCGGAAAAACCTCTATCGc                                                                                                                             >  1:597619/1‑150 (MQ=255)
                        cTTACCCCGAAAATGGCGCCCACAAACCTTTGCTGACGTCGTCGGCCAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTAATCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGc                                                                                                                             <  2:250333/150‑1 (MQ=255)
                         ttAGCCCGAAAATGGCGCCCACAAACCTTTGCTGACGTCGTCGGCCAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGcc                                                                                                                            <  2:438816/150‑1 (MQ=255)
                         ttAGCCCGAAAATGGCGCCCACAAACCTTTGCTGACGTCGTCGGCCAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGcc                                                                                                                            <  1:1801895/150‑1 (MQ=255)
                          tAGCCCGAAAATGGCGCCCACAAACCTTTGCTGACGTCGTCGGCCAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGccc                                                                                                                           >  2:1525159/1‑150 (MQ=255)
                           aGCCCGAAAATGGCGCCCACAAACCATTGCTGACGTCGTCGGCCAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTAGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACATCTATCGCCCg                                                                                                                          >  1:313334/1‑150 (MQ=255)
                            gCCCGAAAATGGCGCCCACAAACCTTTGCTGACGTCGTCGGCCAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGa                                                                                                                         >  2:1479871/1‑150 (MQ=255)
                               cGAAAATGGCGCCCACAAACCTTTGCTGACGTCGTCGGCCAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGActg                                                                                                                      >  2:688299/1‑150 (MQ=255)
                                       gcgcCCACATACCTTTGCTGACGTCGTCGGCCATGAACATGTGCTGACCGCACTGGCGAACGGCTTTTCGTTAGGGCGTATTCATCATTATTATCTTTTTTCCGTCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGaa                                                                                                              <  2:339035/150‑1 (MQ=255)
                                         gcCCACAAACCTTTGCTGACGTCGTCGGCCAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCAAGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAgg                                                                                                            <  1:896442/150‑1 (MQ=255)
                                          cccACAATCCTTTGCTGACGTCGTCGGCCAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGTGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAggg                                                                                                           <  1:854238/150‑1 (MQ=255)
                                          cccACAAACCTTTGCTGACGTCGTCGGCCAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAggg                                                                                                           >  1:1692576/1‑150 (MQ=255)
                                          cccACAAACCTTTGCTGACGTCGTCGGCCAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAggg                                                                                                           <  1:1665457/150‑1 (MQ=255)
                                          cccACAAACCTTTGCTGACGTCGTCGGCCAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAggg                                                                                                           >  2:101392/1‑150 (MQ=255)
                                          cccACAAACCTTTGCTGACGTCGTCGGCCAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAggg                                                                                                           >  2:800941/1‑150 (MQ=255)
                                           ccACAAACCTTTGCTGACGTCGTCGGCCAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAgggg                                                                                                          >  2:1040053/1‑150 (MQ=255)
                                            cacaAACCTTTGCTGACGTCGTCGGCCAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGc                                                                                                         >  1:406519/1‑150 (MQ=255)
                                              caAACCTTTGCTGACGTCGTCGGCCAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTa                                                                                                       >  2:1086651/1‑150 (MQ=255)
                                               aaaCCTTTGCTGACGTCGTCGGCCAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTaa                                                                                                      <  1:806791/150‑1 (MQ=255)
                                               aaaCCTTTGCTGACGTCGTCGGCCAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTaa                                                                                                      >  2:1490803/1‑150 (MQ=255)
                                                     ttGCTGACGTCGTCGGCCAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCg                                                                                                <  1:1746817/150‑1 (MQ=255)
                                                     ttGCTGACGTCGTCGGCCAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCg                                                                                                <  1:653731/150‑1 (MQ=255)
                                                     ttGCTGACGTCGTCGGCCAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGGCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCg                                                                                                <  2:18073/150‑1 (MQ=255)
                                                     ttGCTGACGTCGTCGGCCAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGGCGTTAGGGCGTATTCATCATGCTTATGTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCg                                                                                                <  2:1266944/150‑1 (MQ=255)
                                                        cTGACGTCGTCGGCCAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGCCTGCTGGCGAAGGGGCTAAACTGCGaaa                                                                                             >  2:954289/1‑150 (MQ=255)
                                                        cTGACGTCGTCGGCCAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGaaa                                                                                             >  1:956195/1‑150 (MQ=255)
                                                         tGACGTCGTCGGCCAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAAc                                                                                            >  2:1359707/1‑150 (MQ=255)
                                                           aCGTCGTCGGCCAGGAACATGAGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCg                                                                                          <  1:907016/150‑1 (MQ=255)
                                                            cgtcgtCGGCCAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCgg                                                                                         <  1:55401/150‑1 (MQ=255)
                                                            cgtcgtCGGCCAGGAACATGTGCTGAACGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGATGGCGAAGGGGCTAAACTGCGAAACCgg                                                                                         >  1:147310/1‑150 (MQ=255)
                                                              tcgtcgGCCAGGAACATGTTCTGACAGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGTCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCa                                                                                       <  2:161184/150‑1 (MQ=255)
                                                                   ggCCAGGATCATGTGCTGACCTGACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAATCCTCTATCGCCCGACTGCTGGCGAAGTGGCTAAACTGCGAAACCGGCATTAcc                                                                                  <  1:436221/150‑1 (MQ=255)
                                                                     ccAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTAATCATCATGCTTATCTTTTTTACGGCACCCGTGGCGTCGGAAAAACCTCAATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTACcgc                                                                                >  1:875291/1‑150 (MQ=255)
                                                                      cAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTACcgcg                                                                               <  1:1657548/150‑1 (MQ=255)
                                                                       aGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTCGCGTCGGAAAAACCTCTATCGCCCGACTGCGGGCGAAGGGGCTAAACTGCGAACCCGGCATTACCGCGa                                                                              <  1:1032680/150‑1 (MQ=255)
                                                                               gtgCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTACCGCGACGCCGTgc                                                                      <  1:1728190/150‑1 (MQ=255)
                                                                                 gCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTACCGCGACGCCGTgcgg                                                                    >  2:1714394/1‑150 (MQ=255)
                                                                                 gCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTACCGCGACGCCGTgcgg                                                                    >  1:1125340/1‑150 (MQ=255)
                                                                                 gCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCGCTATCGCCCGACTGATGGCGAAGGGGCTAAACTGCGCCTCCGGCAGTACCTCGCAGCCGTgcgg                                                                    >  2:270677/1‑150 (MQ=255)
                                                                                   tGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTTGCGAAGGGGCTAAACTGCGAAACCGGCATTACCGCGACGCCTTgcggcg                                                                  >  1:301300/1‑150 (MQ=255)
                                                                                   tGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTACCTCGACGCCGTgcggcg                                                                  <  1:688299/150‑1 (MQ=255)
                                                                                      ccGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTACCGCGACGCCGTGCGGCgtgt                                                               >  1:77027/1‑150 (MQ=255)
                                                                                        gCACTGGCGTACGGCTTGTCGGTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTAGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGTGGCTAAACTGCTAAACCGGCATTACCGCGACGCCGTGCGGCGTGTGc                                                             <  2:1147033/150‑1 (MQ=255)
                                                                                           cTGGCGAATGGCTTGTCGTTAGGTCGTATTCATCATGCTTATCTTTTTTCCTGCTCCCGTGGCGTCGTAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGTATAAACTTCGAAACCGGATTTACCGCGACGCCGTGCGGCGTGTGCGAt                                                          <  1:1315333/150‑1 (MQ=255)
                                                                                           cTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCTGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTACCGCGACGCCGTGCGGCGTGTGCGAt                                                          <  2:1256649/150‑1 (MQ=255)
                                                                                            gggCGAACGGCTTGTCGGTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTACCGCGACGCCGTGCGGCGTGTGCGATa                                                         <  1:1595632/149‑1 (MQ=255)
                                                                                             ggCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTACCGCGACGCCGTGCGGCGTGTGCGATaa                                                        >  1:1267057/1‑150 (MQ=255)
                                                                                             ggCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTACCGCGACGCCGTGCGGCGTGTGCGATaa                                                        <  1:1003676/150‑1 (MQ=255)
                                                                                               cGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTACCGCGACGCCGTGCGGCGTGTGCGATAACt                                                      <  1:1802247/150‑1 (MQ=255)
                                                                                               cGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACATCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAGCCGGCATTACCGCGACGCCGTGCGGCGTGTGCGATAACt                                                      >  2:1283519/1‑150 (MQ=255)
                                                                                                           cGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTACCGCGACGCCGTGCGGCGTGTGCGATAACTGTCGTGAAATCg                                          <  1:35629/150‑1 (MQ=255)
                                                                                                           cGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTACCGCGACGCCGTGCGGCGTGTGCGATAACTGTCGTGAAATCg                                          <  1:265701/150‑1 (MQ=255)
                                                                                                            gTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCGCGACTGCTGGAGAAGGGGCTATACTGCGAAACCGGCATTACCGCGACGCCGTGCGGCGGGTGCGATAACTGTCGTGACATCgc                                         >  1:101730/1‑149 (MQ=255)
                                                                                                            gTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTACCGCGACGCCGTGCGGCGTGTGCGATAACTGTCGTGAAATCGa                                         >  1:39873/1‑150 (MQ=255)
                                                                                                            gTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTACCGCGACGCCGTGCGGCGTGTACGATAACTGTCGTGAAATCGa                                         >  2:1363798/1‑150 (MQ=255)
                                                                                                            gTTAGGGCGTATACATCATGCTTATCATATTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCTACTGCTGGCGAAGGGGCTAAACTGCTAAACCGGCAATACCGCGACGCCGTGCGGCGTGTGCGATAACTGTCGTGAAATcta                                         >  1:1073730/1‑148 (MQ=255)
                                                                                                               aGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTACCGCGACGCCGTGCGGCGTGTGCGATAACTGTCGTGAAATCGAGCa                                      <  1:1134524/150‑1 (MQ=255)
                                                                                                                gggCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTACCGCGACGCCGTGCGGCGTGTGCGATAACTGTCGTGAAATCGAGCAg                                     >  2:428275/1‑150 (MQ=255)
                                                                                                                gggCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTACCGCGACGCCGTGCGGCGTGTGCGATAACTGTCGTGAAATCGAGCAg                                     <  2:406519/150‑1 (MQ=255)
                                                                                                                     tATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTACCGCGACGCCGTGCGGCGTGTGCGATAACTGTCGTGAAATCGAGCAGGGgcg                                >  2:393039/1‑150 (MQ=255)
                                                                                                                      aTTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTACCGCGACGCCGTGCGGCGTGTGCGATAACTGTCGTGAAATCGAGCAGGGgcgc                               >  1:1599690/1‑150 (MQ=255)
                                                                                                                       ttCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTACCGCGACGCCGTGCGGCGTGTGCGATAACTGTCGTGAAATCGAGCAGGGGCGCt                              >  2:1389149/1‑150 (MQ=255)
                                                                                                                       ttCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTACCGCGACGCCGTGCGGCGTGTGCGATAACTGTCGTGAAATCGAGCAGGGGCGCt                              >  1:1061733/1‑150 (MQ=255)
                                                                                                                       ttCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCGAAACTGCGAAACCGGCATTACCGCGACGCCGTGCGGCGTGTGCGATAACTGTCGTGAAATCGAGCAGGGGCGCt                              <  1:738793/150‑1 (MQ=255)
                                                                                                                               gCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTACCGCGACGCCGTGCGGCGTGTGCGATAACTGTCGTGAAATCGAGCAGGGGCGCTTTGTCGAt                      <  1:1714394/150‑1 (MQ=255)
                                                                                                                               gCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTACCGCGACGCCGTGCGGCGTGTGCGATAACTGTCGTGAAATCGAGCAGGGGCGCTTTGTCGAt                      >  1:1027638/1‑150 (MQ=255)
                                                                                                                                cTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTACCGCGACGCCGTGCGGCGTGTGCGATAACTGTCTTGAAATCGAGCAGGGGCGCTTTGTCGATc                     <  1:180949/150‑1 (MQ=255)
                                                                                                                                cTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTACCGCGACGCCGTGCGGCGTGTGCGATAACTGTCGTGAAATCGAGCAGGGGCGCTTTGTCGATc                     <  1:1479871/150‑1 (MQ=255)
                                                                                                                                cTTATCTTTTATCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTACCGCGACGCCGTGCGGCGTGTGCGATAACTGTCGTGAAATCGAGCAGGGGCGCTTTGTCGATc                     <  1:1304444/150‑1 (MQ=255)
                                                                                                                                 ttATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTACCGCGACGCCGTGCGGCGTGTGCGATAACTGTCGTGAAATCGAGCAGGGGCGCTTTGTCGATCt                    <  2:1360928/150‑1 (MQ=255)
                                                                                                                                 ttATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTACCGCGACGCCGTGCGGCGTGTGCGATAACTGTCGTGAAATCGAGCAGGGGCGCTTTGTCGATCt                    <  2:1333706/150‑1 (MQ=255)
                                                                                                                                   aTCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTACCGCGACGCCGTGCGGCGTGTGCGATAACTGTCGTGAAATCGAGCAGGGGCGCTTTGTCGATCTGa                  <  2:1692576/150‑1 (MQ=255)
                                                                                                                                     cTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTACCGCGACGCCGTGCGGCGTGTGCGATAACTGTCGTGATATCGAGCAGGGGCGCGTTGTCGATCTGAtt                >  1:469812/1‑150 (MQ=255)
                                                                                                                                     cTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTACCGCGACGCCGTGCGGCGTGTGCGATAACTGTCGTGAAATCGAGCAGGGGCGCTTTGTCGATCTGAtt                >  2:1085689/1‑150 (MQ=255)
                                                                                                                                     cTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTACCGCGACGCCGTGCGGCGTGTGCGATAACTGTCGTGAAATCGAGCAGGGGCGCTTTGTCGATCTGAtt                >  1:1051118/1‑150 (MQ=255)
                                                                                                                                     cTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTACCGCGACGCCGTGCGGCGTGTGCGATAACTGTCGTGAAATCGAGCAGGGGCGCTTTGTCGATCTGAtt                >  2:880086/1‑150 (MQ=255)
                                                                                                                                     cTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTACCGCGACGCCGTGCGGCGTGTGCGATAACTGTCGTGAAATCGAGCAGGGGCGCTTTGTCGATCTGAtt                >  1:391962/1‑150 (MQ=255)
                                                                                                                                         gttCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTACCGCGACGCCGTGCGGCGTGTGCGATAACTGTCGTGACAGCGAGCGGGGGCGCGTTGTCGAGCTGATTGcaa            >  1:727083/2‑150 (MQ=255)
                                                                                                                                          ttCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTTAACTGCGAAACCGGCATTACCGCGACCCCGTGCCGCCTGTCCGATAACTCTCGTGAAATCGAGCAGCTGCGCTTTGTCGATCCGATTGAAAt           >  2:1093589/1‑150 (MQ=255)
                                                                                                                                            ccGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTACCGCGACGCCGTGCGGCGTGTGCGATAACTGTCGTGAAATCGAGCAGGGGCGCTTTGTCGATCTGATTGAAATCg         >  1:1659355/1‑150 (MQ=255)
                                                                                                                                             cGGCACCAGTGGCGTCGGAAAACCCTCTTTCGCCCGACTGCTGGCGAAGGGGCGAAACTGCGAAACCGGCATTTCCGCGACGCCGGGAGGCGTGTGCGATAACTGTCGTGAAATCGAGCAGGGGCGCTTTGTCGATCTGATTGAAAtagc        >  2:1466957/1‑147 (MQ=255)
                                                                                                                                                  cccGTGGCGTCGGAAAAACCTCTATCGCCGGACTGCTGGCGATGGGGCTAACCTGCGAAACCGGCTTTACCGCGACGCCGTGCGGCGTGTGCCATAACTGTCGTGAAATCGAGCAGCGGAGCTTTGTCGATCTGATTGAACTCGAccccg   >  2:900610/1‑150 (MQ=255)
                                                                                                                                                   ccGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTACCGCGACGCCGTGCGGCGTGTGCGATAACTGTCGTGAAATCGAGCAGGGGCGCTTTGTCGATCTGATTGAAATCGAcgccgc  >  1:638930/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GAGCCTGCCAATGAGTTATCAGGTCTTAGCCCGAAAATGGCGCCCACAAACCTTTGCTGACGTCGTCGGCCAGGAACATGTGCTGACCGCACTGGCGAACGGCTTGTCGTTAGGGCGTATTCATCATGCTTATCTTTTTTCCGGCACCCGTGGCGTCGGAAAAACCTCTATCGCCCGACTGCTGGCGAAGGGGCTAAACTGCGAAACCGGCATTACCGCGACGCCGTGCGGCGTGTGCGATAACTGTCGTGAAATCGAGCAGGGGCGCTTTGTCGATCTGATTGAAATCGACGCCGC  >  NC_000913/492082‑492378

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: