Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,390,401 C→A 7.2% intergenic (‑41/+56) psd ← / ← rsgA phosphatidylserine decarboxylase/ribosome small subunit‑dependent GTPase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,390,4010CA 7.2% 471.2 / 18.3 160intergenic (‑41/+56)psd/rsgAphosphatidylserine decarboxylase/ribosome small subunit‑dependent GTPase A
Reads supporting (aligned to +/- strand):  ref base C (72/76);  new base A (5/7);  total (77/83)
Fisher's exact test for biased strand distribution p-value = 7.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

Only 100 of 172 total aligned reads displayed.

CCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTGATAGTTTTCGAAACGGGTTTCCGCGATT  >  NC_000913/4390252‑4390550
                                                                                                                                                     |                                                                                                                                                     
ccAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCTCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGcc                                                                                                                                                       <  2:1556598/150‑1 (MQ=255)
ccAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGTTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGcc                                                                                                                                                       >  1:1229545/1‑150 (MQ=255)
ccAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGcc                                                                                                                                                       <  2:541117/150‑1 (MQ=255)
ccAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGcc                                                                                                                                                       >  1:965795/1‑150 (MQ=255)
ccAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGcc                                                                                                                                                       >  2:1595669/1‑150 (MQ=255)
ccAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGcc                                                                                                                                                       <  2:1799287/150‑1 (MQ=255)
ccAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGACATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGcc                                                                                                                                                       >  2:1017728/1‑150 (MQ=255)
 cAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGACAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCt                                                                                                                                                      <  2:360776/150‑1 (MQ=255)
   gTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTAc                                                                                                                                                    >  1:1006904/1‑150 (MQ=255)
    ttttGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTTAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACa                                                                                                                                                   >  1:1048882/1‑150 (MQ=255)
     tttGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACAt                                                                                                                                                  >  2:844127/1‑150 (MQ=255)
       tGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGc                                                                                                                                                >  2:1344080/1‑150 (MQ=255)
        gTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGcc                                                                                                                                               >  2:1307906/1‑150 (MQ=255)
          cAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCgg                                                                                                                                             >  2:613898/1‑150 (MQ=255)
          cAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGAAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCgg                                                                                                                                             >  1:1400180/1‑150 (MQ=255)
          cAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAAATTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCgg                                                                                                                                             <  2:705323/150‑1 (MQ=255)
             ccATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATc                                                                                                                                          <  2:1777086/150‑1 (MQ=255)
              cATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATcc                                                                                                                                         <  1:533228/150‑1 (MQ=255)
              cATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATcc                                                                                                                                         <  2:410828/150‑1 (MQ=255)
                 ccTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGa                                                                                                                                      <  2:1074170/150‑1 (MQ=255)
                 ccTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCATGTAGGCCGGATcagga                                                                                                                                      <  1:1395529/150‑5 (MQ=255)
                  cTGCCCGCTTGCTTGTGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCGGATCCGGCATGTAGGCCGGATcaggaa                                                                                                                                     <  2:143490/150‑6 (MQ=255)
                  cTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGaa                                                                                                                                     >  1:1320119/1‑150 (MQ=255)
                   tGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGaaa                                                                                                                                    <  1:1168901/150‑1 (MQ=255)
                         cttgcttgCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAgg                                                                                                                              <  1:481153/150‑1 (MQ=255)
                          ttgcttgcGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAggg                                                                                                                             <  2:1342228/150‑1 (MQ=255)
                             cttgcGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGATTTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATACGGCCTACATGCCGGATCCTGAAAAAAAGGGGAc                                                                                                                          >  1:1090305/1‑150 (MQ=255)
                             cttgcGCCCCAACCCGCCACGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTTAATGAATTTATCAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTTCATGCCGGATCCTGAAAAAAAGGGGAc                                                                                                                          <  1:1206741/150‑1 (MQ=255)
                              ttgcGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACg                                                                                                                         >  1:1141701/1‑150 (MQ=255)
                               tgcGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTCTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTg                                                                                                                                       >  1:322270/1‑135 (MQ=255)
                                gcgcCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGAt                                                                                                                       >  2:1764010/1‑150 (MQ=255)
                                    cccAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTa                                                                                                                   <  1:1489939/150‑1 (MQ=255)
                                         cccGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAacgac                                                                                                              <  2:1398635/150‑1 (MQ=255)
                                          ccGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAacgacg                                                                                                             <  2:1019070/150‑1 (MQ=255)
                                           cGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACgg                                                                                                            >  1:772661/1‑150 (MQ=255)
                                                   gAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTa                                                                                                    >  2:821269/1‑150 (MQ=255)
                                                     gTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAAt                                                                                                  <  2:1231432/150‑1 (MQ=255)
                                                       aaGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTGCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTg                                                                                                <  1:1736528/150‑1 (MQ=255)
                                                       aaGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTg                                                                                                <  2:542969/150‑1 (MQ=255)
                                                       aaGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTg                                                                                                <  1:905660/150‑1 (MQ=255)
                                                         gCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTgtc                                                                                              <  1:1524727/150‑1 (MQ=255)
                                                         cccATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGTCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTttc                                                                                              <  1:120668/149‑1 (MQ=255)
                                                           cATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTgtcag                                                                                            <  1:234706/150‑1 (MQ=255)
                                                           cATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTgtcag                                                                                            <  1:251484/150‑1 (MQ=255)
                                                                 ttCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATcc                                                                                      >  2:167575/1‑150 (MQ=255)
                                                                    ggCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTa                                                                                   >  1:404239/1‑150 (MQ=255)
                                                                      cAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATc                                                                                 <  1:571520/150‑1 (MQ=255)
                                                                       aGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCa                                                                                >  1:1343748/1‑150 (MQ=255)
                                                                        taaTGTACTTTAGCGATAGTTTAAATGAATTTAACAAGGTAGCCTCCAGTCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAg                                                                               <  2:424805/149‑1 (MQ=255)
                                                                          aTGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGCTCCGGCCTACATGCCGGATCCCGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGaa                                                                             <  1:1125634/150‑1 (MQ=255)
                                                                               cTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGtt                                                                        >  1:572256/1‑150 (MQ=255)
                                                                                  tAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGttttt                                                                     <  1:749605/150‑1 (MQ=255)
                                                                                          gTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGttt                                                             >  1:952824/1‑150 (MQ=255)
                                                                                          gTTTAAATGAATTTAACAAGGTAGCCACCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTAAATTCCGGATCCTGAAAAAAAGGGGAAGATTCTAACGAGGGTTAGGTTAATTGTCAGTCATCCGATTCAGAAAAGTTTTTACGCGtgt                                                             >  1:1015058/1‑148 (MQ=255)
                                                                                            ttAAATGAATTAAACAAGGTAGCCTCCAGGCCATTGGTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGttttt                                                           <  2:1617124/150‑1 (MQ=255)
                                                                                                 tGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTg                                                      <  1:986995/150‑1 (MQ=255)
                                                                                                   aaTTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTgcg                                                    >  2:779525/1‑150 (MQ=255)
                                                                                                    aTTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATTCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTgcgc                                                   <  2:1128313/150‑1 (MQ=255)
                                                                                                       tAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAACGGGGACGATTCTAACGACGGTTAGCTTAATTGTCACTCATCCGTATAAGAAAAGTTTTTACGCGTTTTTACCTGCGCCaa                                                >  2:1539159/1‑149 (MQ=255)
                                                                                                       tAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCAt                                                >  2:1140876/1‑150 (MQ=255)
                                                                                                        aaCAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGTTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATg                                               >  2:1719708/1‑150 (MQ=255)
                                                                                                        aaCAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATg                                               <  2:1053863/150‑1 (MQ=255)
                                                                                                        aaCAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGAAGGTTAGCTTAATTGACAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTTAGCCATg                                               >  2:976130/1‑150 (MQ=255)
                                                                                                         aCAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGCGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGc                                              <  2:1191849/150‑1 (MQ=255)
                                                                                                            aGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCttt                                           <  1:869117/150‑1 (MQ=255)
                                                                                                            aGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCAACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACTCGTTTTTACCTGCGCCATGCttt                                           >  1:751692/1‑150 (MQ=255)
                                                                                                              gTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTcc                                         <  1:344221/150‑1 (MQ=255)
                                                                                                               tAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCa                                        >  2:936306/1‑150 (MQ=255)
                                                                                                               tAGCCTCCAGGCCATTGTTTTGTAGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCa                                        <  1:686844/150‑1 (MQ=255)
                                                                                                                aGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAg                                       <  2:1671500/150‑1 (MQ=255)
                                                                                                                 gCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGa                                      <  2:1006904/150‑1 (MQ=255)
                                                                                                                     ccAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATAc                                  <  1:1751972/150‑1 (MQ=255)
                                                                                                                     ccAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATAc                                  >  2:1406385/1‑150 (MQ=255)
                                                                                                                       aGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACgg                                >  2:44748/1‑150 (MQ=255)
                                                                                                                        ggCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACTGt                               >  1:370208/1‑150 (MQ=255)
                                                                                                                        ggCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGt                               >  1:668030/1‑150 (MQ=255)
                                                                                                                         gCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTg                              <  1:1307906/150‑1 (MQ=255)
                                                                                                                               gTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTGATAGtt                        >  1:1687642/1‑150 (MQ=255)
                                                                                                                                ttttGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTGATAGttt                       >  1:85965/1‑150 (MQ=255)
                                                                                                                                 tttGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTGATAGtttt                      >  1:467362/1‑150 (MQ=255)
                                                                                                                                  ttGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTGATAGTTTTc                     <  1:821269/150‑1 (MQ=255)
                                                                                                                                  ttGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTGATAGTTTTc                     <  2:729746/150‑1 (MQ=255)
                                                                                                                                   tGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAACGCGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTGTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTGATAGTTTTCg                    <  2:1434552/150‑1 (MQ=255)
                                                                                                                                   tGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTGATAGTTTTCg                    >  2:1574728/1‑150 (MQ=255)
                                                                                                                                    gTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTGATAGTTTTCGa                   <  1:277289/150‑1 (MQ=255)
                                                                                                                                    gTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTGATAGTTTTCGa                   >  2:1364739/1‑150 (MQ=255)
                                                                                                                                         tCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTGATAGTTTTCGAAACgg              >  1:484348/1‑150 (MQ=255)
                                                                                                                                          ccTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTGATAGTTTTCGAAACggg             >  2:320145/1‑150 (MQ=255)
                                                                                                                                          caggATCCGGCATGTAGGCCGGATCCTGAAAAAAAGGTGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTGATAGTTTTCGAAACggg             >  2:131075/4‑150 (MQ=255)
                                                                                                                                           cTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTGATAGTTTTCGAAACGGGt            >  2:27339/1‑150 (MQ=255)
                                                                                                                                           cTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTGATAGTTTTCGAAACGGGt            >  2:285109/1‑150 (MQ=255)
                                                                                                                                           cTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTAAGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGAGATAGTTTTCGAAACTGGt            >  1:1402501/1‑150 (MQ=255)
                                                                                                                                            tGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCAATGCTTTCCAGAATATGGTGATTGTTTTCGAAACGGGtt           >  2:1660203/1‑150 (MQ=255)
                                                                                                                                             gATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGAGCCATGATATCCAGAATAAGGAGATAGTTTTCGAAACGGGttt          >  1:152545/1‑150 (MQ=255)
                                                                                                                                                ccGGCCTAATTGCCGGATCCTGAAAAATAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTGATAGTTTTCGAAACGGGTTTCcg       <  2:1090305/150‑1 (MQ=255)
                                                                                                                                                 cGGCCTACATGCCGGATCCTGAAAAAAAGGGGCCGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTGATAGATTTCGAAACGGGTTTCcgc      >  1:232142/1‑150 (MQ=255)
                                                                                                                                                  ggCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGATTTCCAGAATACGGTGATAGTTTTCGAAACTGGTTTCcgcg     >  2:1102907/1‑150 (MQ=255)
                                                                                                                                                  ggCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGAAGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTGATAGTTTTCGAAACGGGTTTCcgcg     >  2:581879/1‑150 (MQ=255)
                                                                                                                                                    ccTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTGATAGTTTTCGAAACGGGTTTCCGCGAt   >  1:1630290/1‑150 (MQ=255)
                                                                                                                                                     cTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTGATAGTTTTCGAAACGGGTTTCCGCGAtt  >  2:759071/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CCAGTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTGATAGTTTTCGAAACGGGTTTCCGCGATT  >  NC_000913/4390252‑4390550

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: