Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,390,404 C→T 7.4% intergenic (‑44/+53) psd ← / ← rsgA phosphatidylserine decarboxylase/ribosome small subunit‑dependent GTPase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,390,4040CT 7.4% 497.4 / 16.9 162intergenic (‑44/+53)psd/rsgAphosphatidylserine decarboxylase/ribosome small subunit‑dependent GTPase A
Reads supporting (aligned to +/- strand):  ref base C (74/74);  new base T (5/7);  total (80/82)
Fisher's exact test for biased strand distribution p-value = 7.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01

Only 100 of 164 total aligned reads displayed.

GTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTGATAGTTTTCGAAACGGGTTTCCGCGATTTTC  >  NC_000913/4390255‑4390553
                                                                                                                                                     |                                                                                                                                                     
gTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTAc                                                                                                                                                       >  1:1006904/1‑150 (MQ=255)
 ttttGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTTAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACa                                                                                                                                                      >  1:1048882/1‑150 (MQ=255)
   ttGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATg                                                                                                                                                    >  2:1004593/1‑150 (MQ=255)
    tGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGc                                                                                                                                                   >  2:1344080/1‑150 (MQ=255)
       cAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCgg                                                                                                                                                >  2:613898/1‑150 (MQ=255)
       cAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGAAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCgg                                                                                                                                                >  1:1400180/1‑150 (MQ=255)
       cAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAAATTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCgg                                                                                                                                                <  2:705323/150‑1 (MQ=255)
          ccATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATc                                                                                                                                             <  2:1777086/150‑1 (MQ=255)
          ccATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTGCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCATGTAGGCCGGATc                                                                                                                                             <  1:1162215/150‑1 (MQ=255)
           cATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATcc                                                                                                                                            <  2:769633/150‑1 (MQ=255)
           cATCCTCCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGCTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCATGTAGGCCGGATca                                                                                                                                            <  1:340895/150‑2 (MQ=255)
              ccTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATACTGa                                                                                                                                         >  2:112757/1‑150 (MQ=255)
                       ttgcttgcGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAggg                                                                                                                                <  2:1527633/150‑1 (MQ=255)
                       ttgcttgcGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAggg                                                                                                                                <  1:757145/150‑1 (MQ=255)
                        tgcttgcGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAgggg                                                                                                                               >  1:1343280/1‑150 (MQ=255)
                          cttgcGCCCCAACCCGCCACGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTTAATGAATTTATCAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTTCATGCCGGATCCTGAAAAAAAGGGGAc                                                                                                                             <  1:1206741/150‑1 (MQ=255)
                           ttgcGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACg                                                                                                                            >  1:1141701/1‑150 (MQ=255)
                            tgcGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTCTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTg                                                                                                                                          <  2:322270/135‑1 (MQ=255)
                            tgcGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTCTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTg                                                                                                                                          >  1:322270/1‑135 (MQ=255)
                             gcgcACCAACCCGCCAGGCGAGTAATCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAATTGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGAt                                                                                                                          <  2:475598/150‑1 (MQ=255)
                              cgcCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGAtt                                                                                                                         >  1:1671500/1‑150 (MQ=255)
                                ccccAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCt                                                                                                                       >  1:480788/1‑150 (MQ=255)
                                      cccGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAacgac                                                                                                                 <  1:553432/150‑1 (MQ=255)
                                       ccGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAacgacg                                                                                                                <  2:1019070/150‑1 (MQ=255)
                                        cGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACgg                                                                                                               >  1:772661/1‑150 (MQ=255)
                                          ccAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGtt                                                                                                             <  2:1017013/150‑1 (MQ=255)
                                              gCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCt                                                                                                         >  2:1343877/1‑150 (MQ=255)
                                               ggattAAGCCATAGTTTCGGCAGAATGTACTTTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCtt                                                                                                        <  2:1568164/146‑1 (MQ=255)
                                               cGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGTCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCtt                                                                                                        >  1:43606/1‑150 (MQ=255)
                                                gAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTa                                                                                                       >  2:821269/1‑150 (MQ=255)
                                                    aaGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACATGTTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTg                                                                                                   <  2:378512/150‑1 (MQ=255)
                                                    aaGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTGCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTg                                                                                                   <  1:1736528/150‑1 (MQ=255)
                                                    aaGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTg                                                                                                   <  1:905660/150‑1 (MQ=255)
                                                    aaGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTg                                                                                                   <  2:542969/150‑1 (MQ=255)
                                                      gCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTgtc                                                                                                 <  1:1524727/150‑1 (MQ=255)
                                                       ccATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTgtca                                                                                                >  1:862428/1‑150 (MQ=255)
                                                       ccATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTgtca                                                                                                <  2:1320119/150‑1 (MQ=255)
                                                       ccATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTgtca                                                                                                >  1:139892/1‑150 (MQ=255)
                                                        cATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTgtcag                                                                                               <  1:251484/150‑1 (MQ=255)
                                                        cATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTgtcag                                                                                               <  1:234706/150‑1 (MQ=255)
                                                              ttCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATcc                                                                                         >  2:167575/1‑150 (MQ=255)
                                                                 ggCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTa                                                                                      >  2:1727907/1‑150 (MQ=255)
                                                                 ggCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTa                                                                                      >  1:404239/1‑150 (MQ=255)
                                                                  gCAGAATGTACTGTAGCGAAACTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGCACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTAt                                                                                     <  2:1244083/150‑1 (MQ=255)
                                                                    aGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCa                                                                                   >  1:1343748/1‑150 (MQ=255)
                                                                     taaTGTACTTTAGCGATAGTTTAAATGAATTTAACAAGGTAGCCTCCAGTCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAg                                                                                  <  2:424805/149‑1 (MQ=255)
                                                                       aTGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGCTCCGGCCTACATGCCGGATCCCGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGaa                                                                                <  1:1125634/150‑1 (MQ=255)
                                                                       aTGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGaa                                                                                <  2:184268/150‑1 (MQ=255)
                                                                            cTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGtt                                                                           >  1:572256/1‑150 (MQ=255)
                                                                               tAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGttttt                                                                        <  1:749605/150‑1 (MQ=255)
                                                                                  cGAAAGTTTTAATGAATTAAACAAGGTAGCCTACAGTCTATTGTTTTTTCGTTCGTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGTGGACGATTCTAACGACGGTTAGATTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTAcg                                                                     <  1:151028/150‑1 (MQ=255)
                                                                                    aaaGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTAcgcg                                                                   <  1:1203792/150‑1 (MQ=255)
                                                                                       gTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGttt                                                                >  1:952824/1‑150 (MQ=255)
                                                                                       gTTTAAATGAATTTAACAAGGTAGCCACCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTAAATTCCGGATCCTGAAAAAAAGGGGAAGATTCTAACGAGGGTTAGGTTAATTGTCAGTCATCCGATTCAGAAAAGTTTTTACGCGtgt                                                                >  1:1015058/1‑148 (MQ=255)
                                                                                        tttAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGtttt                                                               <  1:605385/150‑1 (MQ=255)
                                                                                         ttAAATGAATTAAACAAGGTAGCCTCCAGGCCATTGGTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGttttt                                                              <  2:1617124/150‑1 (MQ=255)
                                                                                              tGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTg                                                         <  2:1065668/150‑1 (MQ=255)
                                                                                                aaTTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTgcg                                                       >  2:779525/1‑150 (MQ=255)
                                                                                                aaTTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTCTCAGTCAACCGTATCAGAAAAGTTTTTACGCTTTTTTTCCTgcg                                                       >  1:521055/1‑150 (MQ=255)
                                                                                                    tAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAACGGGGACGATTCTAACGACGGTTAGCTTAATTGTCACTCATCCGTATAAGAAAAGTTTTTACGCGTTTTTACCTGCGCCaa                                                   >  2:1539159/1‑149 (MQ=255)
                                                                                                     aaaaaGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATTCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATg                                                  <  2:875588/147‑1 (MQ=255)
                                                                                                     aaCAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGTTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATg                                                  >  2:1719708/1‑150 (MQ=255)
                                                                                                     aaCAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATg                                                  <  2:1053863/150‑1 (MQ=255)
                                                                                                     aaCAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGAAGGTTAGCTTAATTGACAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTTAGCCATg                                                  >  2:976130/1‑150 (MQ=255)
                                                                                                      aCAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGCGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGc                                                 <  2:1191849/150‑1 (MQ=255)
                                                                                                         aGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCttt                                              <  1:869117/150‑1 (MQ=255)
                                                                                                         aGGCAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCttt                                              <  2:1400180/150‑1 (MQ=255)
                                                                                                           gTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTcc                                            <  2:1611658/150‑1 (MQ=255)
                                                                                                            tAGCCTCCTGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCa                                           <  1:680344/150‑1 (MQ=255)
                                                                                                            tAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCa                                           >  2:751441/1‑150 (MQ=255)
                                                                                                            tAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCa                                           >  2:936306/1‑150 (MQ=255)
                                                                                                            tAGCCTCCAGGCCATTGTTTTGTAGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCa                                           <  1:686844/150‑1 (MQ=255)
                                                                                                             aGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAg                                          <  2:1671500/150‑1 (MQ=255)
                                                                                                             aGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAg                                          <  1:1595669/150‑1 (MQ=255)
                                                                                                                cTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAAt                                       >  1:981565/1‑150 (MQ=255)
                                                                                                                  ccAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATAc                                     >  2:1406385/1‑150 (MQ=255)
                                                                                                                    aGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACgg                                   >  2:44748/1‑150 (MQ=255)
                                                                                                                     ggCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACTGt                                  >  1:370208/1‑150 (MQ=255)
                                                                                                                      gCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTg                                 <  1:1307906/150‑1 (MQ=255)
                                                                                                                            gTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTGATAGtt                           >  1:204105/1‑150 (MQ=255)
                                                                                                                            gTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTGATAGtt                           >  1:1687642/1‑150 (MQ=255)
                                                                                                                             ttttGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTGATAGttt                          >  1:1426373/1‑150 (MQ=255)
                                                                                                                               ttGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTGATAGTTTTc                        <  1:821269/150‑1 (MQ=255)
                                                                                                                                tGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAACGCGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTGTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTGATAGTTTTCg                       <  2:1434552/150‑1 (MQ=255)
                                                                                                                                 gTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAACAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTGATAGTTTTCGa                      <  2:1138050/150‑1 (MQ=255)
                                                                                                                                 gTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTGATAGTTTTCGa                      >  2:1364739/1‑150 (MQ=255)
                                                                                                                                 gTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTGATAGTTTTCGa                      <  1:277289/150‑1 (MQ=255)
                                                                                                                                    tttcaggATCCGGCATGTAGGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTGATAGTTTTCGAAAc                   >  1:1078345/7‑150 (MQ=255)
                                                                                                                                      tCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTGATAGTTTTCGAAACgg                 >  1:484348/1‑150 (MQ=255)
                                                                                                                                      tCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTGATAGTTTTCGAAACgg                 >  2:697528/1‑150 (MQ=255)
                                                                                                                                       caggATCCGGCATGTAGGCCGGATCCTGAAAAAAAGGTGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTGATAGTTTTCGAAACggg                >  2:131075/4‑150 (MQ=255)
                                                                                                                                        cTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTGATAGTTTTCGAAACGGGt               >  2:285109/1‑150 (MQ=255)
                                                                                                                                        cTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTGATAGTTTTCGAAACGGGt               >  2:27339/1‑150 (MQ=255)
                                                                                                                                        aggATCCGGCATGTAGGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTTGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTGATAGTTTTCGAAACGGGt               >  1:1226231/3‑150 (MQ=255)
                                                                                                                                        aggATCCGGCATGTAGGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTGATAGTTTTCGAAACGGGt               >  2:1224416/3‑150 (MQ=255)
                                                                                                                                         tGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCAATGCTTTCCAGAATATGGTGATTGTTTTCGAAACGGGtt              >  2:1660203/1‑150 (MQ=255)
                                                                                                                                             ccGGCCTAATTGCCGGATCCTGAAAAATAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTGATAGTTTTCGAAACGGGTTTCcg          <  2:1090305/150‑1 (MQ=255)
                                                                                                                                              cGGCCTACATGCCGGATCCTGAAAAAAAGGGGCCGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTGATAGATTTCGAAACGGGTTTCcgc         >  1:232142/1‑150 (MQ=255)
                                                                                                                                               ggCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGATTTCCAGAATACGGTGATAGTTTTCGAAACTGGTTTCcgcg        >  2:1102907/1‑150 (MQ=255)
                                                                                                                                               ggCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGAAGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTGATAGTTTTCGAAACGGGTTTCcgcg        >  2:581879/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GTTTTGTCAGCCATCCTGCCCGCTTGCTTGCGCCCCAACCCGCCAGGCGAGTAAGCCATAGTTTCGGCAGAATGTACTGTAGCGAAAGTTTAAATGAATTTAACAAGGTAGCCTCCAGGCCATTGTTTTGTCGTTCCTGATCCGGCCTACATGCCGGATCCTGAAAAAAAGGGGACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAGTTTTTACGCGTTTTTACCTGCGCCATGCTTTCCAGAATACGGTGATAGTTTTCGAAACGGGTTTCCGCGATTTTC  >  NC_000913/4390255‑4390553

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑