Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,414,240 A→T 6.6% intergenic (+49/‑35) yjfC → / → aidB ATP‑Grasp family ATPase/DNA alkylation damage repair protein; flavin‑containing DNA binding protein, weak isovaleryl CoA dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,414,2400AT 6.6% 479.2 / 15.2 164intergenic (+49/‑35)yjfC/aidBATP‑Grasp family ATPase/DNA alkylation damage repair protein; flavin‑containing DNA binding protein, weak isovaleryl CoA dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (83/69);  new base T (6/5);  total (90/74)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01

Only 100 of 167 total aligned reads displayed.

GGATTGTCGATGATGAAGCGTGCGGAATGGGGATCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGG  >  NC_000913/4414091‑4414389
                                                                                                                                                      |                                                                                                                                                     
ggATTGTCGATGATGAAGCGTGCGGAATGGGGATCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATa                                                                                                                                                       >  2:1454512/1‑150 (MQ=255)
 gATTGTCGATGATGAAGCGTGCGGAATGGGGATCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATAc                                                                                                                                                      >  2:1730514/1‑150 (MQ=255)
     gTCGATGATGAAGCGTGCGGAATGGGGATCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCAAACTAAt                                                                                                                                                  >  2:887811/1‑150 (MQ=255)
      tCGATGATGAAGCGTGCGGAATGGGGATCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATg                                                                                                                                                 >  1:401236/1‑150 (MQ=255)
      tCGATGATGAAGCGTGCGGAATGGGGATCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATg                                                                                                                                                 >  1:1283654/1‑150 (MQ=255)
       cGATGATGAAGCGTGCGGAATGGGGATCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTGTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATgg                                                                                                                                                >  2:1295415/1‑150 (MQ=255)
         aggatgtATCGTGTGGTATGGGGAGCCGTGAAGATAACCAACTGATGACCAAAGACACCTCACGGTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTg                                                                                                                                              <  1:1575267/148‑1 (MQ=255)
               aaGCGTGCGGAATGGGGATCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGAGACTGca                                                                                                                                        >  1:1588306/1‑149 (MQ=255)
                  cGTGCGGAATGGGGATCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCAtt                                                                                                                                     <  1:874194/150‑1 (MQ=255)
                  cGTGCGGAATGGGGAGCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTTGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTCACCATTAGTATGGTCACTGCCAtg                                                                                                                                     <  2:1664973/150‑2 (MQ=255)
                    tGCGGAATGGGGATCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGa                                                                                                                                   >  2:1485384/1‑150 (MQ=255)
                     gCGGAATGGGGATCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACAATACTAATGGTGACTGCCATTGAt                                                                                                                                  >  1:1148339/1‑150 (MQ=255)
                            ggggATCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTTGCAATCTCTTTCTGTCATGAATCAATGGCAGTGACCATACTAATGGTGACTGCCATTGATggaggga                                                                                                                           >  2:319693/1‑150 (MQ=255)
                            ggggATCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATggaggga                                                                                                                           >  2:377948/1‑150 (MQ=255)
                             gggATCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGCCCATACTAATGGTGACTGCCATTGATggagggag                                                                                                                          >  2:32063/1‑150 (MQ=255)
                                   cGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAg                                                                                                                    >  1:1188610/1‑150 (MQ=255)
                                    gTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGTATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGt                                                                                                                   <  1:1065086/150‑1 (MQ=255)
                                      gAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGc                                                                                                                 >  2:618796/1‑150 (MQ=255)
                                       aaGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCa                                                                                                                >  2:106134/1‑150 (MQ=255)
                                          ctAACCCACTGCTCCCCAAAGCCACCTCACCTTTCGGTCCGCAGTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACAACTGGGGACTGCCATTGATGGAGGGAGACACAGTGCACTg                                                                                                             <  2:1694906/149‑1 (MQ=255)
                                           tAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATggagggag                                                                                                                          <  2:460779/136‑1 (MQ=255)
                                           tAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATggagggag                                                                                                                          >  1:460779/1‑136 (MQ=255)
                                           tAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTgg                                                                                                            <  2:691285/150‑1 (MQ=255)
                                            aaCACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGc                                                                                                           >  2:1425791/1‑150 (MQ=255)
                                                    gATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTcac                                                                                                   >  2:464603/1‑150 (MQ=255)
                                                     aTCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTcaca                                                                                                  <  2:1014743/150‑1 (MQ=255)
                                                       cACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACAcc                                                                                                >  2:1300158/1‑150 (MQ=255)
                                                        aCCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCCCTGGCAAACTCGCACCg                                                                                               >  1:1125080/1‑150 (MQ=255)
                                                        aCCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCg                                                                                               >  2:1537028/1‑150 (MQ=255)
                                                         ccAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGt                                                                                              >  2:636821/1‑150 (MQ=255)
                                                          cAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGtt                                                                                             >  2:814970/1‑150 (MQ=255)
                                                            aaGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGtttt                                                                                           <  2:1591598/150‑1 (MQ=255)
                                                              gACACCTCACGTTTCGGTCCGCAGTACATTGATTGATAAGAAGTGATACGCAATATCCTTCTGTCATGAATCCATTGCAGTGACCATACTAATGGTGAATTGCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTa                                                                                         <  1:84388/150‑1 (MQ=255)
                                                                 acCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATc                                                                                      <  1:1635479/150‑1 (MQ=255)
                                                                  ccTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCa                                                                                     >  1:646960/1‑150 (MQ=255)
                                                                  ccTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCACTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCa                                                                                     >  1:1026069/1‑150 (MQ=255)
                                                                     cACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAAcc                                                                                  >  1:197189/1‑150 (MQ=255)
                                                                     cACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAAcc                                                                                  <  2:565728/150‑1 (MQ=255)
                                                                         tttCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCtata                                                                              <  1:284988/150‑1 (MQ=255)
                                                                         tttCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCtata                                                                              <  2:126223/150‑1 (MQ=255)
                                                                          ttCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATAc                                                                             >  1:1212454/1‑150 (MQ=255)
                                                                          ttCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATAc                                                                             >  1:563663/1‑150 (MQ=255)
                                                                           tCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATAcc                                                                            <  1:1389528/150‑1 (MQ=255)
                                                                             gTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCAt                                                                          >  2:95725/1‑150 (MQ=255)
                                                                             gTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCAt                                                                          >  1:554868/1‑150 (MQ=255)
                                                                                ccGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTaa                                                                       >  1:1236823/1‑150 (MQ=255)
                                                                                  gCATTACATTGCTGGATTAGAATTTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATAATAATTGTGACTGACATTGATGGAGGTATACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAAt                                                                     <  1:260972/150‑1 (MQ=255)
                                                                                  gCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAAt                                                                     >  2:1707084/1‑150 (MQ=255)
                                                                                   cATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATa                                                                    <  1:713483/150‑1 (MQ=255)
                                                                                   cATTACATTGCTGGATAAGAATGTTTTAGAAATCTCTATCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATa                                                                    <  1:1604567/150‑1 (MQ=255)
                                                                                     ttACATTGCTTGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAAc                                                                  <  1:861517/150‑1 (MQ=255)
                                                                                     ttACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAAc                                                                  <  1:763844/150‑1 (MQ=255)
                                                                                      tACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACa                                                                 >  2:532757/1‑150 (MQ=255)
                                                                                       aCATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAg                                                                >  1:194464/1‑150 (MQ=255)
                                                                                       aCATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAg                                                                <  2:1566377/150‑1 (MQ=255)
                                                                                       aCATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAg                                                                <  2:1199886/150‑1 (MQ=255)
                                                                                        cATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGc                                                               >  2:1635040/1‑150 (MQ=255)
                                                                                             cTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACtt                                                          >  1:453263/1‑150 (MQ=255)
                                                                                             cTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACtt                                                          <  1:558524/150‑1 (MQ=255)
                                                                                               ggATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGAACTGGCAAACTAACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTtat                                                        >  2:1084567/1‑150 (MQ=255)
                                                                                                  tAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATAcc                                                     <  1:1485384/150‑1 (MQ=255)
                                                                                                    aGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTg                                                   <  1:1528705/150‑1 (MQ=255)
                                                                                                      aaTGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTc                                                 <  2:1001928/150‑1 (MQ=255)
                                                                                                      aaTGTTTTAGCAAACTCTTTCTGTCATGAATACATGGCAGTGACCATACTAATTGTGACTGACATTTTTGGATGGATACACATTTCACTTGCAAACTAACACCGTTTTTTATCAACCTATACCATTAAATAACAGCAACTTATACCTGTc                                                 <  1:1122670/150‑1 (MQ=255)
                                                                                                         gTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGa                                              >  1:1272845/1‑150 (MQ=255)
                                                                                                          ttttAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGTGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATAAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGAt                                             >  1:90628/1‑150 (MQ=255)
                                                                                                          tattAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGAt                                             <  1:42573/148‑1 (MQ=255)
                                                                                                            ttAGCATTCTCTTTATGTCATGTATCCATGGCAGTGACCATACTAATGGTGACTGCCTTTGATGGAGGGAGACACAGTGCACTTGCAAACTCACACCGTTTTTAATCAACCTATACCATTATATAACAGCAACTTATACCTGTCTGATgg                                           <  2:1090528/150‑1 (MQ=255)
                                                                                                            ttAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATgg                                           <  1:259698/150‑1 (MQ=255)
                                                                                                                  aaTCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGgcgcgc                                      <  2:400408/150‑1 (MQ=255)
                                                                                                                   aTCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGct                                     >  1:1816057/1‑150 (MQ=255)
                                                                                                                    tctcTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGctc                                    <  1:377948/150‑1 (MQ=255)
                                                                                                                       ctTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGc                                 <  2:428368/150‑1 (MQ=255)
                                                                                                                        tttCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCg                                <  1:923481/150‑1 (MQ=255)
                                                                                                                            tGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACCCCGTTTTTAATCACCCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGc                            >  2:43904/1‑150 (MQ=255)
                                                                                                                             gTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCg                           >  1:1765129/1‑150 (MQ=255)
                                                                                                                              tCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCgg                          <  1:618796/150‑1 (MQ=255)
                                                                                                                               cATGAATCCATGGCAGTGACCATACTTATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATTCCTTTAAATAACAGCAACTTATACCTGTCTGTTGGCGCGCTCTGCGAAGCTGt                         <  1:1650307/150‑1 (MQ=255)
                                                                                                                               cATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGt                         >  1:1725939/1‑150 (MQ=255)
                                                                                                                               cATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGAAGGAGGGAGACACAGTGCCCTGGCAAACTCACACCGTTTTTCATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGt                         <  2:1103258/150‑1 (MQ=255)
                                                                                                                               cATGAATCCATGGCAGTGACCATACTAATGGTGACGGCCATAGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTGTTTAATCAACCAAGACCATTAAATAACAGCAACTTATCCCTGTCTGCTGGCCCGCTCTGAGAAGCGGt                         >  1:920351/1‑150 (MQ=255)
                                                                                                                                ctccaTCAATGGCAGTCACCATTAGTATGGTCACTGCCATGGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTa                        >  1:1748928/5‑150 (MQ=37)
                                                                                                                                 tGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTaa                       >  2:22638/1‑150 (MQ=255)
                                                                                                                                   aaTCCATGGCAGTGGCCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAAcg                     <  2:1220395/150‑1 (MQ=255)
                                                                                                                                    aTCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACCGCAACTTATACCTGTATGATGGCGCGCTCTGCGAAGCGGTAAcgc                    >  1:349739/1‑150 (MQ=255)
                                                                                                                                     tcaaTGGCAGTCACCATTAGTATGGTCACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAAcgcg                   >  2:1211218/4‑150 (MQ=39)
                                                                                                                                     tCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAAcgcg                   <  2:1798888/150‑1 (MQ=255)
                                                                                                                                       aaTGGCAGTCACCATTAGTATGGTCACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTg                 >  2:118435/2‑150 (MQ=255)
                                                                                                                                          ggCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAg              >  2:899184/1‑150 (MQ=255)
                                                                                                                                           gCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAgg             >  2:205761/1‑150 (MQ=255)
                                                                                                                                            cAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGt            >  2:569865/1‑150 (MQ=255)
                                                                                                                                              gTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTgc          >  2:1471087/1‑150 (MQ=255)
                                                                                                                                                 aCCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACCGGCAAACTCCCACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTgctgg       >  1:952315/1‑150 (MQ=255)
                                                                                                                                                   cATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTgctggct     >  1:512105/1‑150 (MQ=255)
                                                                                                                                                   cATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTgctggct     <  2:966492/150‑1 (MQ=255)
                                                                                                                                                    aTACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTgctggctg    >  2:280045/1‑150 (MQ=255)
                                                                                                                                                     tACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGctggctgg   <  2:1283654/150‑1 (MQ=255)
                                                                                                                                                     tACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGctggctgg   >  1:1009265/1‑150 (MQ=255)
                                                                                                                                                     tACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGctggctgg   <  1:1537028/150‑1 (MQ=255)
                                                                                                                                                      aCTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTggg  <  1:83128/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
GGATTGTCGATGATGAAGCGTGCGGAATGGGGATCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGG  >  NC_000913/4414091‑4414389

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: