Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,414,241 C→A 5.8% intergenic (+50/‑34) yjfC → / → aidB ATP‑Grasp family ATPase/DNA alkylation damage repair protein; flavin‑containing DNA binding protein, weak isovaleryl CoA dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,414,2410CA 5.8% 485.0 / 10.4 165intergenic (+50/‑34)yjfC/aidBATP‑Grasp family ATPase/DNA alkylation damage repair protein; flavin‑containing DNA binding protein, weak isovaleryl CoA dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (83/70);  new base A (6/6);  total (89/76)
Fisher's exact test for biased strand distribution p-value = 7.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 166 total aligned reads displayed.

GATTGTCGATGATGAAGCGTGCGGAATGGGGATCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGG  >  NC_000913/4414092‑4414389
                                                                                                                                                      |                                                                                                                                                    
    gTCGATGATGAAGCGTGCGGAATGGGGATCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCAAACTAAt                                                                                                                                                  >  2:887811/1‑150 (MQ=255)
     tCGATGATGAAGCGTGCGGAATGGGGATCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATg                                                                                                                                                 >  1:401236/1‑150 (MQ=255)
     tCGATGATGAAGCGTGCGGAATGGGGATCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATg                                                                                                                                                 >  1:1283654/1‑150 (MQ=255)
      cGATGATGAAGCGTGCGGAATGGGGATCCGTGAAGATAACACACTGATCACCAAAGACACCTCAGGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATgg                                                                                                                                                <  1:919813/150‑1 (MQ=255)
      cGATGATGAAGCGTGCGGAATGGGGATCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTATCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATgg                                                                                                                                                <  1:718593/150‑1 (MQ=255)
         tgatgaAGCGTGCGGAATGGGGATCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGa                                                                                                                                             <  1:1183303/150‑1 (MQ=255)
          gatgaAGCGTGCGGAATGGGGATCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGAc                                                                                                                                            >  2:713483/1‑150 (MQ=255)
             gaAGCGTGCGGAATGGGGATCCGTGAAGATAACACACTGATCACCAAAGACCCCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTGTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGc                                                                                                                                         >  2:329277/1‑150 (MQ=255)
             gaAGCGTGCGGAATGGGGATCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTCACCATTAGTATGGTCACTGc                                                                                                                                         <  2:1284712/150‑1 (MQ=255)
              aaGCGTGCGGAATGGGGATCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGAGACTGca                                                                                                                                        >  1:1588306/1‑149 (MQ=255)
               aGCGTGCGGAATGGGGATCCGTGAAGATAACACACTGATCACCAAAGACACCTCCCGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCa                                                                                                                                       >  2:1681881/1‑150 (MQ=255)
               aGCGTGCGGAATGGGGATCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCAATACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACAATACTAATGGTGACTGCCa                                                                                                                                       >  1:429027/1‑150 (MQ=255)
                gCGTGCGGAATGGGGCTCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCTCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTCACCATTAGTATGGTCACTGCCAt                                                                                                                                      <  2:1208784/150‑1 (MQ=255)
                gCGTGCGGAATGGGGATCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTCACCATTAGTATGGTCACTGCCAt                                                                                                                                      <  2:1074044/150‑1 (MQ=255)
                 cGTGCGGAATGGGGATCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCAtt                                                                                                                                     <  1:874194/150‑1 (MQ=255)
                   tGCGGAATGGGGATCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGa                                                                                                                                   >  2:1476851/1‑150 (MQ=255)
                   tGCGGAATGGGGATCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGa                                                                                                                                   >  2:1485384/1‑150 (MQ=255)
                    gCGGAATGGGGATCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACAATACTAATGGTGACTGCCATTGAt                                                                                                                                  >  1:1148339/1‑150 (MQ=255)
                      ggAATGGGGATCCGTGAAGATAACCCACTGATCACCAACGCCACCTCCGGTTTCGTTCCGCAGTTCATGGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATgg                                                                                                                                >  1:145403/1‑150 (MQ=255)
                           ggggATCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTTGCAATCTCTTTCTGTCATGAATCAATGGCAGTGACCATACTAATGGTGACTGCCATTGATggaggga                                                                                                                           >  2:319693/1‑150 (MQ=255)
                           ggggATCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATggaggga                                                                                                                           >  2:377948/1‑150 (MQ=255)
                              gATCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGac                                                                                                                        <  1:580719/150‑1 (MQ=255)
                                   gTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGTATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGt                                                                                                                   <  1:1065086/150‑1 (MQ=255)
                                     gAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGc                                                                                                                 >  2:618796/1‑150 (MQ=255)
                                        gATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACt                                                                                                              <  1:1818354/150‑1 (MQ=255)
                                         ctAACCCACTGCTCCCCAAAGCCACCTCACCTTTCGGTCCGCAGTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACAACTGGGGACTGCCATTGATGGAGGGAGACACAGTGCACTg                                                                                                             <  2:1694906/149‑1 (MQ=255)
                                          tAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATggagggag                                                                                                                          >  1:460779/1‑136 (MQ=255)
                                          tAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATggagggag                                                                                                                          <  2:460779/136‑1 (MQ=255)
                                          tAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTgg                                                                                                            <  2:1588152/150‑1 (MQ=255)
                                                    aTCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTcaca                                                                                                  <  2:1014743/150‑1 (MQ=255)
                                                      cACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACAcc                                                                                                >  2:1300158/1‑150 (MQ=255)
                                                       aCCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCCCTGGCAAACTCGCACCg                                                                                               >  1:1125080/1‑150 (MQ=255)
                                                       aCCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCg                                                                                               >  2:1537028/1‑150 (MQ=255)
                                                        ccAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGt                                                                                              >  2:636821/1‑150 (MQ=255)
                                                        ccAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGt                                                                                              >  2:1765884/1‑150 (MQ=255)
                                                         cAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGtt                                                                                             >  2:814970/1‑150 (MQ=255)
                                                           aaGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGtttt                                                                                           <  2:1591598/150‑1 (MQ=255)
                                                            aGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGttttt                                                                                          <  2:1048068/150‑1 (MQ=255)
                                                             gACACCTCACGTTTCGGTCCGCAGTACATTGATTGATAAGAAGTGATACGCAATATCCTTCTGTCATGAATCCATTGCAGTGACCATACTAATGGTGAATTGCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTa                                                                                         <  1:84388/150‑1 (MQ=255)
                                                                 ccTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCa                                                                                     >  1:646960/1‑150 (MQ=255)
                                                                 ccTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCACTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCa                                                                                     >  1:1026069/1‑150 (MQ=255)
                                                                   tCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAAc                                                                                   <  2:256731/150‑1 (MQ=255)
                                                                    cACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAAcc                                                                                  >  1:197189/1‑150 (MQ=255)
                                                                    cACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAAcc                                                                                  <  2:920746/150‑1 (MQ=255)
                                                                    cACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAAcc                                                                                  <  2:565728/150‑1 (MQ=255)
                                                                        tttCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCtata                                                                              <  2:126223/150‑1 (MQ=255)
                                                                        tttCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCtata                                                                              <  1:284988/150‑1 (MQ=255)
                                                                         ttCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATAc                                                                             >  1:563663/1‑150 (MQ=255)
                                                                         ttCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATAc                                                                             >  1:1212454/1‑150 (MQ=255)
                                                                          tCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATAcc                                                                            <  1:1389528/150‑1 (MQ=255)
                                                                            gTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCAt                                                                          >  2:95725/1‑150 (MQ=255)
                                                                               ccGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTaa                                                                       >  1:1236823/1‑150 (MQ=255)
                                                                                 gCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAAt                                                                     >  1:35118/1‑150 (MQ=255)
                                                                                 gCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAAt                                                                     >  2:1707084/1‑150 (MQ=255)
                                                                                  cATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATa                                                                    <  1:713483/150‑1 (MQ=255)
                                                                                  cATTACATTGCTGGATAAGAATGTTTTAGAAATCTCTATCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATa                                                                    <  1:1604567/150‑1 (MQ=255)
                                                                                    ttACATTGCTGGATACGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAATCTCACCCCGTTTTTAATCAACCTATACCATTAAATAAc                                                                  <  1:1261595/150‑1 (MQ=255)
                                                                                     tACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACa                                                                 >  2:532757/1‑150 (MQ=255)
                                                                                      aCATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAg                                                                <  2:1199886/150‑1 (MQ=255)
                                                                                       cATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGc                                                               >  2:1635040/1‑150 (MQ=255)
                                                                                            cTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACtt                                                          <  1:558524/150‑1 (MQ=255)
                                                                                             tGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTta                                                         >  2:497507/1‑150 (MQ=255)
                                                                                                   aGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTg                                                   <  1:1528705/150‑1 (MQ=255)
                                                                                                     aaTGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTc                                                 <  1:1730514/150‑1 (MQ=255)
                                                                                                         ttttAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGTGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATAAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGAt                                             >  1:90628/1‑150 (MQ=255)
                                                                                                         tattAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGAt                                             <  1:42573/148‑1 (MQ=255)
                                                                                                           ttAGCATTCTCTTTATGTCATGTATCCATGGCAGTGACCATACTAATGGTGACTGCCTTTGATGGAGGGAGACACAGTGCACTTGCAAACTCACACCGTTTTTAATCAACCTATACCATTATATAACAGCAACTTATACCTGTCTGATgg                                           <  2:1090528/150‑1 (MQ=255)
                                                                                                           ttAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATgg                                           <  1:259698/150‑1 (MQ=255)
                                                                                                               gCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGgcgc                                        >  1:992659/1‑150 (MQ=255)
                                                                                                                 aaTCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGgcgcgc                                      <  2:400408/150‑1 (MQ=255)
                                                                                                                  aTCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGct                                     >  1:1816057/1‑150 (MQ=255)
                                                                                                                     tctTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTg                                  <  1:532757/150‑1 (MQ=255)
                                                                                                                     tctTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTCCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTg                                  <  2:109885/150‑1 (MQ=255)
                                                                                                                       tttCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCg                                <  1:923481/150‑1 (MQ=255)
                                                                                                                          cTGTCTTGAATCCATGTCAGTGTCCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCTACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAg                             <  2:429027/150‑1 (MQ=255)
                                                                                                                           tGTCATGAATCCATGGCAGTGACCATACTAATGTTGACTGCCATTGATGGATGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTATATAACTGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGc                            <  1:1540818/150‑1 (MQ=255)
                                                                                                                           tGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACCCCGTTTTTAATCACCCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGc                            >  2:43904/1‑150 (MQ=255)
                                                                                                                           tGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGc                            <  1:848083/150‑1 (MQ=255)
                                                                                                                            gTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAACCTCACCCCGTTTTTACTCAACCGATACCATGTAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCg                           >  1:1101902/1‑150 (MQ=255)
                                                                                                                            gTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCg                           >  1:1765129/1‑150 (MQ=255)
                                                                                                                              cATGAATCCATGGCAGTGACCATACTTATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATTCCTTTAAATAACAGCAACTTATACCTGTCTGTTGGCGCGCTCTGCGAAGCTGt                         <  1:1650307/150‑1 (MQ=255)
                                                                                                                              cATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGt                         >  2:1450866/1‑150 (MQ=255)
                                                                                                                              cATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGt                         >  1:1725939/1‑150 (MQ=255)
                                                                                                                                tccaTCAATGGCAGTCACCATTAGTATGGTCAATGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAAATTATACCGGTCTGATGGCGCGCTCTGCGAAGCGGTaa                       >  2:1119174/4‑150 (MQ=38)
                                                                                                                                  aaTCCATGGCAGTGGCCATACTAATGGTGACTGCCATTGATGGATGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAAcg                     <  2:1214417/150‑1 (MQ=255)
                                                                                                                                   aTCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACCGCAACTTATACCTGTATGATGGCGCGCTCTGCGAAGCGGTAAcgc                    >  1:349739/1‑150 (MQ=255)
                                                                                                                                    tCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAAcgcg                   <  2:1798888/150‑1 (MQ=255)
                                                                                                                                     ccATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGt                  >  2:1273615/1‑150 (MQ=255)
                                                                                                                                        tGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGaa               <  1:1632471/150‑1 (MQ=255)
                                                                                                                                        tGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGaa               <  2:607741/150‑1 (MQ=255)
                                                                                                                                          gCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAgg             >  2:205761/1‑150 (MQ=255)
                                                                                                                                                aCCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACCGGCAAACTCCCACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTgctgg       >  1:952315/1‑150 (MQ=255)
                                                                                                                                                  cATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTgctggct     >  1:512105/1‑150 (MQ=255)
                                                                                                                                                  cATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTgctggct     <  2:966492/150‑1 (MQ=255)
                                                                                                                                                   aTACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTgctggctg    >  2:280045/1‑150 (MQ=255)
                                                                                                                                                    tACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGTTgctgtctag   >  1:260095/1‑148 (MQ=255)
                                                                                                                                                    tACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGctggctgg   >  2:1418235/1‑150 (MQ=255)
                                                                                                                                                    tACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGctggctgg   >  1:1009265/1‑150 (MQ=255)
                                                                                                                                                    tACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGctggctgg   <  1:1537028/150‑1 (MQ=255)
                                                                                                                                                     aCTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTggg  <  1:83128/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                    
GATTGTCGATGATGAAGCGTGCGGAATGGGGATCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGG  >  NC_000913/4414092‑4414389

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: