Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,414,243 A→T 7.0% intergenic (+52/‑32) yjfC → / → aidB ATP‑Grasp family ATPase/DNA alkylation damage repair protein; flavin‑containing DNA binding protein, weak isovaleryl CoA dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,414,2430AT 7.0% 426.8 / 18.3 166intergenic (+52/‑32)yjfC/aidBATP‑Grasp family ATPase/DNA alkylation damage repair protein; flavin‑containing DNA binding protein, weak isovaleryl CoA dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (83/71);  new base T (6/6);  total (89/77)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.92e-02

Only 100 of 167 total aligned reads displayed.

GTCGATGATGAAGCGTGCGGAATGGGGATCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATA  >  NC_000913/4414096‑4414392
                                                                                                                                                   |                                                                                                                                                     
gTCGATGATGAAGCGTGCGGAATGGGGATCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCAAACTAAt                                                                                                                                                     >  2:887811/1‑150 (MQ=255)
 tCGATGATGAAGCGTGCGGAATGGGGATCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATg                                                                                                                                                    >  1:1283654/1‑150 (MQ=255)
  cGATGATGAAGCGTGCGGAATGGGGATCCGTGAAGATAACACACTGATCACCAAAGACACCTCAGGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATgg                                                                                                                                                   <  1:919813/150‑1 (MQ=255)
  cGATGATGAAGCGTGCGGAATGGGGATCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTGTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATgg                                                                                                                                                   >  2:1295415/1‑150 (MQ=255)
  cGATGATGAAGCGTGCGGAATGGGGATCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTATCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATgg                                                                                                                                                   <  1:718593/150‑1 (MQ=255)
    aggatgtATCGTGTGGTATGGGGAGCCGTGAAGATAACCAACTGATGACCAAAGACACCTCACGGTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTg                                                                                                                                                 <  1:1575267/148‑1 (MQ=255)
         gaAGCGTGCGGAATGGGGATCCGTGAAGATAACACACTGATCACCAAAGACCCCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTGTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGc                                                                                                                                            >  2:329277/1‑150 (MQ=255)
         gaAGCGTGCGGAATGGGGATCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTCACCATTAGTATGGTCACTGc                                                                                                                                            <  2:1284712/150‑1 (MQ=255)
           aGCGTGCGGAATGGGGATCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCAATACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACAATACTAATGGTGACTGCCa                                                                                                                                          >  1:429027/1‑150 (MQ=255)
            gCGTGCGGAATGGGGCTCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCTCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTCACCATTAGTATGGTCACTGCCAt                                                                                                                                         <  2:1208784/150‑1 (MQ=255)
            gCGTGCGGAATGGGGATCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTCACCATTAGTATGGTCACTGCCAt                                                                                                                                         <  2:1074044/150‑1 (MQ=255)
             cGTGCGGAATGGGGAGCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTTGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTCACCATTAGTATGGTCACTGCCAtg                                                                                                                                        <  2:1664973/150‑2 (MQ=255)
               tGCGGAATGGGGATCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGa                                                                                                                                      >  2:1485384/1‑150 (MQ=255)
                gCGGAATGGGGATCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACAATACTAATGGTGACTGCCATTGAt                                                                                                                                     >  1:1148339/1‑150 (MQ=255)
                 cGGAATGGGGATCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATg                                                                                                                                    >  1:1535690/1‑150 (MQ=255)
                 cGGAATGGGGATCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATg                                                                                                                                    <  1:419242/150‑1 (MQ=255)
                       ggggATCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATggaggga                                                                                                                              >  2:377948/1‑150 (MQ=255)
                        gggATCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGCCCATACTAATGGTGACTGCCATTGATggagggag                                                                                                                             >  2:32063/1‑150 (MQ=255)
                          gATCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGac                                                                                                                           <  1:580719/150‑1 (MQ=255)
                              cGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAg                                                                                                                       >  1:1188610/1‑150 (MQ=255)
                               gTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGTATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGt                                                                                                                      <  1:1065086/150‑1 (MQ=255)
                                 gAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGc                                                                                                                    >  2:618796/1‑150 (MQ=255)
                                    gATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACt                                                                                                                 <  1:1818354/150‑1 (MQ=255)
                                     ctAACCCACTGCTCCCCAAAGCCACCTCACCTTTCGGTCCGCAGTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACAACTGGGGACTGCCATTGATGGAGGGAGACACAGTGCACTg                                                                                                                <  2:1694906/149‑1 (MQ=255)
                                      tAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATggagggag                                                                                                                             <  2:460779/136‑1 (MQ=255)
                                      tAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTgg                                                                                                               <  2:691285/150‑1 (MQ=255)
                                               gATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTcac                                                                                                      >  2:464603/1‑150 (MQ=255)
                                                   aCCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCCCTGGCAAACTCGCACCg                                                                                                  >  1:1125080/1‑150 (MQ=255)
                                                   aCCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCg                                                                                                  >  2:1537028/1‑150 (MQ=255)
                                                    ccAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGt                                                                                                 >  1:1798888/1‑150 (MQ=255)
                                                    ccAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGt                                                                                                 >  2:636821/1‑150 (MQ=255)
                                                    ccAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGt                                                                                                 >  2:1765884/1‑150 (MQ=255)
                                                     cAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGtt                                                                                                >  2:814970/1‑150 (MQ=255)
                                                       aaGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGtttt                                                                                              <  2:1591598/150‑1 (MQ=255)
                                                        aGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGttttt                                                                                             <  2:1048068/150‑1 (MQ=255)
                                                            acCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATc                                                                                         <  1:1635479/150‑1 (MQ=255)
                                                             ccTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCACTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCa                                                                                        >  1:1026069/1‑150 (MQ=255)
                                                               tCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAAc                                                                                      <  2:256731/150‑1 (MQ=255)
                                                                cACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAAcc                                                                                     <  2:565728/150‑1 (MQ=255)
                                                                cACGTTTCGTTCCGCATTACATTGCGGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAAcc                                                                                     <  1:842536/150‑1 (MQ=255)
                                                                     ttCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATAc                                                                                >  1:1212454/1‑150 (MQ=255)
                                                                      tCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATAcc                                                                               <  1:1389528/150‑1 (MQ=255)
                                                                        gTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCAt                                                                             >  2:95725/1‑150 (MQ=255)
                                                                        gTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCAt                                                                             >  1:554868/1‑150 (MQ=255)
                                                                           ccGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTaa                                                                          >  1:1236823/1‑150 (MQ=255)
                                                                             gCATTACATTGCTGGATTAGAATTTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATAATAATTGTGACTGACATTGATGGAGGTATACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAAt                                                                        <  1:260972/150‑1 (MQ=255)
                                                                             gCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAAt                                                                        >  2:1707084/1‑150 (MQ=255)
                                                                             gCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAAt                                                                        >  1:35118/1‑150 (MQ=255)
                                                                                ttACATTGCTTGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAAc                                                                     <  1:861517/150‑1 (MQ=255)
                                                                                ttACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAAc                                                                     <  1:763844/150‑1 (MQ=255)
                                                                                 tACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACa                                                                    >  2:532757/1‑150 (MQ=255)
                                                                                  aCATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAg                                                                   <  2:1566377/150‑1 (MQ=255)
                                                                                  aCATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAg                                                                   >  1:194464/1‑150 (MQ=255)
                                                                                   cATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGc                                                                  >  2:1635040/1‑150 (MQ=255)
                                                                                        cTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACtt                                                             >  1:453263/1‑150 (MQ=255)
                                                                                          ggATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGAACTGGCAAACTAACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTtat                                                           >  2:1084567/1‑150 (MQ=255)
                                                                                             tAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATAcc                                                        <  1:1485384/150‑1 (MQ=255)
                                                                                               aGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTg                                                      >  1:472723/1‑150 (MQ=255)
                                                                                                 aaTGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTc                                                    <  2:1001928/150‑1 (MQ=255)
                                                                                                 aaTGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTc                                                    <  1:1730514/150‑1 (MQ=255)
                                                                                                 aaTGTTTTAGCAAACTCTTTCTGTCATGAATACATGGCAGTGACCATACTAATTGTGACTGACATTTTTGGATGGATACACATTTCACTTGCAAACTAACACCGTTTTTTATCAACCTATACCATTAAATAACAGCAACTTATACCTGTc                                                    <  1:1122670/150‑1 (MQ=255)
                                                                                                    gTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGa                                                 >  1:1272845/1‑150 (MQ=255)
                                                                                                     ttttAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGAt                                                >  1:65595/1‑150 (MQ=255)
                                                                                                          gCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGAGACAGTGCACTGGCAACCTCACCCCGTTTTTAATCAGCCTATTCCATGAAAGAAGAGCAAGTTATACCTGTGTGATGgcgc                                           >  2:307854/1‑150 (MQ=255)
                                                                                                          gCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGgcgc                                           >  1:992659/1‑150 (MQ=255)
                                                                                                           cAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGgcgcg                                          >  1:215481/1‑150 (MQ=255)
                                                                                                            aaTCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGgcgcgc                                         <  2:400408/150‑1 (MQ=255)
                                                                                                              tctcTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGctc                                       <  1:377948/150‑1 (MQ=255)
                                                                                                                tctTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTg                                     <  1:532757/150‑1 (MQ=255)
                                                                                                                tctTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTCCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTg                                     <  2:109885/150‑1 (MQ=255)
                                                                                                                 ctTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGc                                    <  1:1591640/150‑1 (MQ=255)
                                                                                                                 ctTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGc                                    <  2:428368/150‑1 (MQ=255)
                                                                                                                   ttCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAAATCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGa                                  >  1:165329/1‑150 (MQ=255)
                                                                                                                      tGTCATGAATCCATGGCAGTGACCATACTAATGTTGACTGCCATTGATGGATGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTATATAACTGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGc                               <  1:1540818/150‑1 (MQ=255)
                                                                                                                      tGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACCCCGTTTTTAATCACCCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGc                               >  2:43904/1‑150 (MQ=255)
                                                                                                                      tGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGc                               <  1:848083/150‑1 (MQ=255)
                                                                                                                        tCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCgg                             <  1:618796/150‑1 (MQ=255)
                                                                                                                         cctccaTCAATGGCAGTCACCATTAGTATGGTCACTGCCATGGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGt                            >  1:29656/6‑150 (MQ=37)
                                                                                                                         cATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGt                            >  2:1450866/1‑150 (MQ=255)
                                                                                                                         cATGAATCCATGGCAGTGACCATACTAATGGTGACGGCCATAGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTGTTTAATCAACCAAGACCATTAAATAACAGCAACTTATCCCTGTCTGCTGGCCCGCTCTGAGAAGCGGt                            >  1:920351/1‑150 (MQ=255)
                                                                                                                          ctccaTCAATGGCAGTCACCATTAGTATGGTCACTGCCATGGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTa                           >  1:1748928/5‑150 (MQ=37)
                                                                                                                           tccaTCAATGGCAGTCACCATTAGTATGGTCAATGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAAATTATACCGGTCTGATGGCGCGCTCTGCGAAGCGGTaa                          >  2:1119174/4‑150 (MQ=38)
                                                                                                                           tGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTaa                          >  2:22638/1‑150 (MQ=255)
                                                                                                                             aaTCCATGGCAGTGGCCATACTAATGGTGACTGCCATTGATGGATGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAAcg                        <  2:1214417/150‑1 (MQ=255)
                                                                                                                             aaTCCATGGCAGTGGCCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAAcg                        <  2:1220395/150‑1 (MQ=255)
                                                                                                                              aTCAATGGCAGTCACCATTAGTATGGTCACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAAcgc                       >  1:562082/1‑150 (MQ=39)
                                                                                                                                ccATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGt                     >  2:1273615/1‑150 (MQ=255)
                                                                                                                                 aaTGGCAGTCACCATTAGTATGGTCACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTg                    >  2:118435/2‑150 (MQ=255)
                                                                                                                                   tGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGaa                  <  1:1632471/150‑1 (MQ=255)
                                                                                                                                    ggCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAg                 >  2:899184/1‑150 (MQ=255)
                                                                                                                                     gCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAgg                >  1:935669/1‑150 (MQ=255)
                                                                                                                                      cAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGt               >  2:569865/1‑150 (MQ=255)
                                                                                                                                        gTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTgc             >  2:1471087/1‑150 (MQ=255)
                                                                                                                                           aCCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTTGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATCCCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTgctgg          <  1:887811/150‑1 (MQ=255)
                                                                                                                                             cATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTgctggct        <  2:966492/150‑1 (MQ=255)
                                                                                                                                               tACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGTTgctgtctag      >  1:260095/1‑148 (MQ=255)
                                                                                                                                               tACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGctggctgg      >  2:1418235/1‑150 (MQ=255)
                                                                                                                                               tACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGctggctgg      >  1:1009265/1‑150 (MQ=255)
                                                                                                                                                aCTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTggg     <  2:197189/150‑1 (MQ=255)
                                                                                                                                                   aaTGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATa  >  1:1466084/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
GTCGATGATGAAGCGTGCGGAATGGGGATCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATTGATGGAGGGAGACACAGTGCACTGGCAAACTCACACCGTTTTTAATCAACCTATACCATTAAATAACAGCAACTTATACCTGTCTGATGGCGCGCTCTGCGAAGCGGTAACGCGTGAAGGTGCTGGCTGGGATA  >  NC_000913/4414096‑4414392

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: