Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,510,334 G→T 6.3% intergenic (+201/+356) yjhV → / ← fecE pseudogene, KpLE2 phage‑like element/ferric citrate ABC transporter ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,510,3340GT 6.3% 378.7 / 8.7 133intergenic (+201/+356)yjhV/fecEpseudogene, KpLE2 phage‑like element/ferric citrate ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base G (64/60);  new base T (4/5);  total (68/65)
Fisher's exact test for biased strand distribution p-value = 7.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 136 total aligned reads displayed.

CACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCAGAGTTCAAAATTGATTGCCG  >  NC_000913/4510185‑4510482
                                                                                                                                                     |                                                                                                                                                    
cacaCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTgg                                                                                                                                                      <  1:602399/150‑1 (MQ=255)
cacaCTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTgg                                                                                                                                                      <  2:1183803/150‑1 (MQ=255)
    cTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCATAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGcaca                                                                                                                                                  <  1:1634820/150‑1 (MQ=255)
        ttAGAAAAATCGATTGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGc                                                                                                                                              <  2:628473/150‑1 (MQ=255)
         tAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCg                                                                                                                                             >  1:1777647/1‑150 (MQ=255)
           taaaaaTCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGTGCCAGAGCgga                                                                                                                                           <  1:666322/149‑3 (MQ=255)
           gAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGaa                                                                                                                                           >  2:517622/1‑150 (MQ=255)
           gAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGaa                                                                                                                                           >  2:1402615/1‑150 (MQ=255)
           gAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGaa                                                                                                                                           >  2:836425/1‑150 (MQ=255)
           gAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGGCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGaa                                                                                                                                           <  1:105049/150‑1 (MQ=255)
             aaaaTCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGTGCCAGAGCggaca                                                                                                                                         <  1:1645479/150‑5 (MQ=255)
               aaTCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGTGCCAGAGCggacatt                                                                                                                                       <  2:411250/150‑7 (MQ=255)
                  cGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAAtttttt                                                                                                                                    >  1:1814241/1‑150 (MQ=255)
                   caTGGTCAGTTACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGAGCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCTGCTCCGGGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTg                                                                                                                                   <  1:971006/149‑1 (MQ=255)
                                tGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGa                                                                                                                      <  1:535770/150‑1 (MQ=255)
                                 ggATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGaa                                                                                                                     <  1:412972/150‑1 (MQ=255)
                                   aTTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAAt                                                                                                                   <  2:1181554/150‑1 (MQ=255)
                                   aTTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAAt                                                                                                                   <  2:421639/150‑1 (MQ=255)
                                     tAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGATCTGGCACAGAGCGAAATTTTTTTATATCCCCCCTGAAATCt                                                                                                                 >  1:21035/1‑150 (MQ=255)
                                      ggCAATTCCGTTCCATGGATGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAAATTTTTGATCTCACCACTCAAAtata                                                                                                                >  2:416892/2‑150 (MQ=255)
                                      aGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTa                                                                                                                >  2:272929/1‑150 (MQ=255)
                                      aGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTa                                                                                                                >  2:1692662/1‑150 (MQ=255)
                                       gCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTaa                                                                                                               >  2:597327/1‑150 (MQ=255)
                                       gCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTaa                                                                                                               <  1:1272979/150‑1 (MQ=255)
                                         aaTTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATTTCCGCACTGGCACAGAGCGAAATTTTTTGATATCACCCCTGAAATCTaaaa                                                                                                             >  2:1518825/1‑149 (MQ=255)
                                         aaTTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGACATCTAAAc                                                                                                             >  2:1336802/1‑150 (MQ=255)
                                            tCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTa                                                                                                          >  1:1127306/1‑150 (MQ=255)
                                             ccGTTCCTTGGTTGCTTTTGCTTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAg                                                                                                         <  2:329212/150‑1 (MQ=255)
                                             ccGTTCCATGGTTTCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAg                                                                                                         <  2:1522965/150‑1 (MQ=255)
                                             ccGTTCCATGGTGGATTTTGATTTACGTTGGCGTCTGATCCTTGATTTTTCCTCAAAATCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAg                                                                                                         <  2:287664/150‑1 (MQ=255)
                                                  ccATGGTTGCTTTTGATTTACGTTGGCGGCTGATCATTGATTTATCCTCAATAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATg                                                                                                    <  2:1112743/150‑1 (MQ=255)
                                                   cATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGt                                                                                                   >  2:1762766/1‑150 (MQ=255)
                                                      ggTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCAc                                                                                                >  2:1089076/1‑150 (MQ=255)
                                                      ggTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAACTTAGTCATGTCACg                                                                                               >  2:725595/1‑150 (MQ=255)
                                                           cTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATATTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGtttt                                                                                           >  2:800961/1‑150 (MQ=255)
                                                            ttttGATTTACGTTGGCTTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGttttt                                                                                          <  2:1510692/150‑1 (MQ=255)
                                                             tttGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTg                                                                                         <  1:732885/150‑1 (MQ=255)
                                                             tttGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTAAGTCATGTCACGTTTTTg                                                                                         >  1:523922/1‑150 (MQ=255)
                                                             tttGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCAAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACATAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTg                                                                                         <  1:416892/150‑1 (MQ=255)
                                                               tGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTggg                                                                                       <  2:40709/150‑1 (MQ=255)
                                                                           gCGTCTGATCATTGATTTATCCTCAAAAGCACATCCTCATTGGTAATGAAACAGCTCCGTGAATGTCCGCTCTGGCTCAGAGCGAAATTTTTTGATCTCACCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAAttt                                                                           <  1:125125/150‑1 (MQ=255)
                                                                            cGTCTGTTCATTGATTTATCCTCAATAGCCCAACCTCATTTGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAATTCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAAtttt                                                                          <  1:302527/150‑1 (MQ=255)
                                                                            cGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAAtttt                                                                          >  1:1251433/1‑150 (MQ=255)
                                                                             gTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTa                                                                         <  2:1811288/150‑1 (MQ=255)
                                                                             gTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGGCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTATACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTa                                                                         <  2:937211/150‑1 (MQ=255)
                                                                              tCTTATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTaa                                                                        <  2:1269487/150‑1 (MQ=255)
                                                                               cTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAAc                                                                       <  2:1739844/150‑1 (MQ=255)
                                                                                   tCATTGATTTATCCTCAAAAGCCCCAACTTATTTGTAATGTACCCGCTCAGTGGATGTCCGCGATGGCCCATTTCGTAATTTTTTGCTCTACCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTcg                                                                   <  1:181818/150‑1 (MQ=255)
                                                                                    cATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTcgc                                                                  <  1:836425/150‑1 (MQ=255)
                                                                                     aTTGATTTATCCTCAAAAGGCCAAGCTCATTGGTTATGATCCAGCTCCGTGAATGTCCGCTCTGGCACAGATCGTAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCTTGGCACGTTTTTGGGTTTCTAAAATTTTAACTTcgcg                                                                 <  1:573881/150‑1 (MQ=255)
                                                                                     aTTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTcgcg                                                                 <  2:444317/150‑1 (MQ=255)
                                                                                     aTTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTACGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTATTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTcgcg                                                                 >  1:1005578/1‑150 (MQ=255)
                                                                                        gATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGttt                                                              >  2:440896/1‑150 (MQ=255)
                                                                                        gATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGttt                                                              >  2:1158073/1‑150 (MQ=255)
                                                                                        gATTTATCCTCAAAAGCCCAACCTCATAGGTAATGAACCAGCTCCGTGAATGACCGCTCTGGCACAGAGCGAAGTTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCCTGTCCCGTATTTGGGTTTCTAAAATTTTAAATACGCtttt                                                              >  1:582087/1‑150 (MQ=255)
                                                                                         aTTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGtttt                                                             >  2:532743/1‑150 (MQ=255)
                                                                                            tATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCg                                                          <  2:781767/150‑1 (MQ=255)
                                                                                             aTCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGt                                                         <  1:707953/150‑1 (MQ=255)
                                                                                               ccTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTg                                                       >  1:1682696/1‑150 (MQ=255)
                                                                                                cTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGc                                                      >  2:973574/1‑150 (MQ=255)
                                                                                                  cAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCg                                                    >  1:812039/1‑150 (MQ=255)
                                                                                                   aaaaGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCGCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGt                                                   <  1:29580/150‑1 (MQ=255)
                                                                                                       gCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAgg                                               >  2:1816955/1‑150 (MQ=255)
                                                                                                        cccAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAAATTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAggt                                              >  1:654902/1‑149 (MQ=255)
                                                                                                         ccAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGt                                             >  1:1470680/1‑150 (MQ=255)
                                                                                                          cAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTTTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGtt                                            >  1:755911/1‑150 (MQ=255)
                                                                                                          cAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGtt                                            >  1:1700024/1‑150 (MQ=255)
                                                                                                             ccTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTtata                                         >  1:1077217/1‑150 (MQ=255)
                                                                                                             ccTCAATGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAAGTTAGTCATGTCACGTTATTGGGTTTCTAAAATTTTAACTTCGCGTTTATCGTTGCCGTAAGGGTtata                                         <  1:273365/150‑1 (MQ=255)
                                                                                                                   tGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGtaa                                   >  1:283454/1‑150 (MQ=255)
                                                                                                                    ggTAATGAACCAGCTCCGTGAATGTCCGCTCTGGTACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAAt                                  >  2:1696395/1‑150 (MQ=255)
                                                                                                                    ggTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCCTGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAAt                                  >  1:469105/1‑150 (MQ=255)
                                                                                                                    ggTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAAt                                  >  2:661814/1‑150 (MQ=255)
                                                                                                                     gTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATg                                 <  2:871110/150‑1 (MQ=255)
                                                                                                                          gAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGt                            >  1:506141/1‑150 (MQ=255)
                                                                                                                          gAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGt                            >  1:455828/1‑150 (MQ=255)
                                                                                                                           aaCCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATAc                                        >  1:651374/1‑137 (MQ=255)
                                                                                                                           aaCCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATAc                                        <  2:651374/137‑1 (MQ=255)
                                                                                                                            aCCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGTTa                          <  1:272929/150‑1 (MQ=255)
                                                                                                                               aGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGTTAAGc                       <  2:1419853/150‑1 (MQ=255)
                                                                                                                                gCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCa                      >  1:1517314/1‑150 (MQ=255)
                                                                                                                                gCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCa                      <  1:1793867/150‑1 (MQ=255)
                                                                                                                                 cTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCag                     <  2:1701300/150‑1 (MQ=255)
                                                                                                                                 cTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCag                     >  2:174291/1‑150 (MQ=255)
                                                                                                                                   ccGTGAATGTCCTCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCagag                   <  1:1334713/150‑1 (MQ=255)
                                                                                                                                   ccGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCagag                   <  1:1401661/150‑1 (MQ=255)
                                                                                                                                   ccGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCagag                   <  1:1762766/150‑1 (MQ=255)
                                                                                                                                   ccGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGGAAGGGTTATACAGAAATGTCCGTTAAGCagag                   >  1:1624391/1‑150 (MQ=255)
                                                                                                                                   ccGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGGTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCagag                   >  1:1115444/1‑150 (MQ=255)
                                                                                                                                      tGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCAGAGtta                >  1:735322/1‑149 (MQ=255)
                                                                                                                                      tGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCAGAGTTc                >  1:367135/1‑150 (MQ=255)
                                                                                                                                         aatttcGCTCTGTGCCAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCAGAGTTCaaa             >  1:1396884/6‑150 (MQ=255)
                                                                                                                                           gTCCGCTCTGGCGCAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGGTGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCAGAGTTCAAAat           >  2:620345/1‑150 (MQ=255)
                                                                                                                                           gTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCAGAGTTCAAAat           >  2:1034700/1‑150 (MQ=255)
                                                                                                                                           gTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCAGAGTTCAAAat           <  2:523922/150‑1 (MQ=255)
                                                                                                                                             tcGCTCTGTGCCAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCAACTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCAGAGTTAAAAattt         >  2:605047/2‑149 (MQ=255)
                                                                                                                                              cGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAACTCTAAACTTATTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCTCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCAGAGTTCAAAattga        <  1:1041386/150‑1 (MQ=255)
                                                                                                                                               gCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGTGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCAGAGTTCAAAattgat       <  1:1820738/150‑1 (MQ=255)
                                                                                                                                                  ctGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCAGAGTTCAAAATTGATTGc    <  1:747657/150‑1 (MQ=255)
                                                                                                                                                  ctGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGATTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCAGAGTTCAAAATTGATTGc    >  2:135471/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCAGAGTTCAAAATTGATTGCCG  >  NC_000913/4510185‑4510482

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: