Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,510,336 A→C 6.6% intergenic (+203/+354) yjhV → / ← fecE pseudogene, KpLE2 phage‑like element/ferric citrate ABC transporter ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,510,3360AC 6.6% 358.5 / 11.3 131intergenic (+203/+354)yjhV/fecEpseudogene, KpLE2 phage‑like element/ferric citrate ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base A (64/56);  new base C (3/6);  total (68/63)
Fisher's exact test for biased strand distribution p-value = 3.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01

Only 100 of 134 total aligned reads displayed.

CACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCAGAGTTCAAAATTGATTGCCGTG  >  NC_000913/4510187‑4510484
                                                                                                                                                     |                                                                                                                                                    
cacTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGca                                                                                                                                                      >  1:1522965/1‑150 (MQ=255)
  cTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCATAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGcaca                                                                                                                                                    <  1:1634820/150‑1 (MQ=255)
      ttAGAAAAATCGATTGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGc                                                                                                                                                <  2:628473/150‑1 (MQ=255)
        aGAAAAATCGATCGTCAGAAACTGGATTAGCAATTCCGTTCCATGGATGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGTGCCAGAGCgg                                                                                                                                              <  2:1031499/150‑2 (MQ=255)
         taaaaaTCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGTGCCAGAGCgga                                                                                                                                             <  1:666322/149‑3 (MQ=255)
         gAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGTGCCAGAGCgga                                                                                                                                             <  2:244237/150‑3 (MQ=255)
         gAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGaa                                                                                                                                             >  2:1402615/1‑150 (MQ=255)
         gAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGaa                                                                                                                                             >  2:836425/1‑150 (MQ=255)
         gAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGaa                                                                                                                                             >  2:517622/1‑150 (MQ=255)
         gAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGGCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGaa                                                                                                                                             <  1:105049/150‑1 (MQ=255)
           aaaaTCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGTGCCAGAGCggaca                                                                                                                                           <  1:1645479/150‑5 (MQ=255)
             aaTCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGTGCCAGAGCggacatt                                                                                                                                         <  2:411250/150‑7 (MQ=255)
                cGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAAtttttt                                                                                                                                      >  1:1814241/1‑150 (MQ=255)
                 gATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTg                                                                                                                                     <  2:1260633/150‑1 (MQ=255)
                 gATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGAGTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAAttttttt                                                                                                                                     >  2:153904/1‑149 (MQ=255)
                 caTGGTCAGTTACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGAGCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCTGCTCCGGGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTg                                                                                                                                     <  1:971006/149‑1 (MQ=255)
                             cTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTCCGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTg                                                                                                                         <  2:1131861/150‑1 (MQ=255)
                              tGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGa                                                                                                                        <  1:535770/150‑1 (MQ=255)
                                 aTTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAAt                                                                                                                     <  2:421639/150‑1 (MQ=255)
                                 aTTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAAt                                                                                                                     <  2:1181554/150‑1 (MQ=255)
                                   tAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGATCTGGCACAGAGCGAAATTTTTTTATATCCCCCCTGAAATCt                                                                                                                   >  1:21035/1‑150 (MQ=255)
                                    aGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTa                                                                                                                  >  2:1692662/1‑150 (MQ=255)
                                    aGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTa                                                                                                                  >  2:272929/1‑150 (MQ=255)
                                     gCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTaa                                                                                                                 >  2:597327/1‑150 (MQ=255)
                                     gCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTaa                                                                                                                 <  1:1272979/150‑1 (MQ=255)
                                       aaTTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATTTCCGCACTGGCACAGAGCGAAATTTTTTGATATCACCCCTGAAATCTaaaa                                                                                                               >  2:1518825/1‑149 (MQ=255)
                                       aaTTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAAc                                                                                                               >  2:1793867/1‑150 (MQ=255)
                                          tCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTa                                                                                                            >  1:1127306/1‑150 (MQ=255)
                                           ccGTTCCATGGTGGATTTTGATTTACGTTGGCGTCTGATCCTTGATTTTTCCTCAAAATCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAg                                                                                                           <  2:287664/150‑1 (MQ=255)
                                            cGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCAAATTTTGTTATTTTTTTATGTGACCACTGATATCTAAACTTAGt                                                                                                          <  2:1388827/150‑1 (MQ=255)
                                                ccATGGTTGCTTTTGATTTACGTTGGCGGCTGATCATTGATTTATCCTCAATAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATg                                                                                                      <  2:1112743/150‑1 (MQ=255)
                                                  aTGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTc                                                                                                    >  1:191011/1‑150 (MQ=255)
                                                    ggTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCAc                                                                                                  >  2:1089076/1‑150 (MQ=255)
                                                    ggTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAACTTAGTCATGTCACg                                                                                                 >  2:725595/1‑150 (MQ=255)
                                                         cTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGtttt                                                                                             >  2:477144/1‑150 (MQ=255)
                                                         cTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATATTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGtttt                                                                                             >  2:800961/1‑150 (MQ=255)
                                                          ttttGATTTACGTTGGCTTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGttttt                                                                                            <  2:1510692/150‑1 (MQ=255)
                                                           tttGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTg                                                                                           <  1:732885/150‑1 (MQ=255)
                                                           tttGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTAAGTCATGTCACGTTTTTg                                                                                           >  1:523922/1‑150 (MQ=255)
                                                           tttGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCAAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACATAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTg                                                                                           <  1:416892/150‑1 (MQ=255)
                                                             tGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTggg                                                                                         <  1:517622/150‑1 (MQ=255)
                                                             tGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTggg                                                                                         <  2:40709/150‑1 (MQ=255)
                                                               ttttACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGtt                                                                                       <  2:589253/149‑1 (MQ=255)
                                                                  tACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCt                                                                                    >  1:781767/1‑150 (MQ=255)
                                                                         gCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAAttt                                                                             <  2:1428403/150‑1 (MQ=255)
                                                                         gCGTCTGATCATTGATTTATCCTCAAAAGCACATCCTCATTGGTAATGAAACAGCTCCGTGAATGTCCGCTCTGGCTCAGAGCGAAATTTTTTGATCTCACCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAAttt                                                                             <  1:125125/150‑1 (MQ=255)
                                                                          cGTCTGTTCATTGATTTATCCTCAATAGCCCAACCTCATTTGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAATTCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAAtttt                                                                            <  1:302527/150‑1 (MQ=255)
                                                                          cGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAAtttt                                                                            >  1:1251433/1‑150 (MQ=255)
                                                                           gTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTa                                                                           <  2:1811288/150‑1 (MQ=255)
                                                                           gTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGGCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTATACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTa                                                                           <  2:937211/150‑1 (MQ=255)
                                                                            tCTTATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTaa                                                                          <  2:1269487/150‑1 (MQ=255)
                                                                             cTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAAc                                                                         <  2:1739844/150‑1 (MQ=255)
                                                                                aTCATTGATTTATCCTCAAAAGCCCCACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGTCACAGAGCGAAATTTTTTGATCGCCACCCTGAAATCTAAACTTAGTCATGTCACGGTTTTGGGTTTCTTAAATTTTAACTTc                                                                      >  1:280515/1‑150 (MQ=255)
                                                                                aTCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCATTAAATCTAAAATTAGTCATGTCACGTTTTTGGTTTTATTAAATTTTAACTTc                                                                      >  1:704239/1‑150 (MQ=255)
                                                                                  cATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTcgc                                                                    <  1:836425/150‑1 (MQ=255)
                                                                                   aTTGATTTATCCTCAAAAGGCCAAGCTCATTGGTTATGATCCAGCTCCGTGAATGTCCGCTCTGGCACAGATCGTAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCTTGGCACGTTTTTGGGTTTCTAAAATTTTAACTTcgcg                                                                   <  1:573881/150‑1 (MQ=255)
                                                                                   aTTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTACGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTATTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTcgcg                                                                   >  1:1005578/1‑150 (MQ=255)
                                                                                      gATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGttt                                                                >  2:440896/1‑150 (MQ=255)
                                                                                      gATTTATCCTCAAAAGCCCAACCTCATAGGTAATGAACCAGCTCCGTGAATGACCGCTCTGGCACAGAGCGAAGTTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCCTGTCCCGTATTTGGGTTTCTAAAATTTTAAATACGCtttt                                                                >  1:582087/1‑150 (MQ=255)
                                                                                          tATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCg                                                            <  2:781767/150‑1 (MQ=255)
                                                                                              cTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGc                                                        >  2:973574/1‑150 (MQ=255)
                                                                                                 aaaaGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGt                                                     <  2:397115/150‑1 (MQ=255)
                                                                                                      cccAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAAATTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAggt                                                >  1:654902/1‑149 (MQ=255)
                                                                                                       ccAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGt                                               >  1:1470680/1‑150 (MQ=255)
                                                                                                        cAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTTTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGtt                                              >  1:755911/1‑150 (MQ=255)
                                                                                                        cAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGtt                                              >  1:1700024/1‑150 (MQ=255)
                                                                                                        cAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCACCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGtt                                              >  1:336441/1‑150 (MQ=255)
                                                                                                           ccTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTtata                                           >  1:1077217/1‑150 (MQ=255)
                                                                                                           ccTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATAc                                          <  2:1722933/150‑1 (MQ=255)
                                                                                                           ccTCAATGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAAGTTAGTCATGTCACGTTATTGGGTTTCTAAAATTTTAACTTCGCGTTTATCGTTGCCGTAAGGGTtata                                           <  1:273365/150‑1 (MQ=255)
                                                                                                                 tGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGtaa                                     >  1:283454/1‑150 (MQ=255)
                                                                                                                  ggTAATGAACCAGCTCCGTGAATGTCCGCTCTGGTACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAAt                                    >  2:1696395/1‑150 (MQ=255)
                                                                                                                  ggTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCCTGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAAt                                    >  1:469105/1‑150 (MQ=255)
                                                                                                                  ggTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAAt                                    >  2:661814/1‑150 (MQ=255)
                                                                                                                   gTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATg                                   <  2:871110/150‑1 (MQ=255)
                                                                                                                    tAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGt                                  >  1:1766021/1‑150 (MQ=255)
                                                                                                                      aTGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTcc                                <  1:604305/150‑1 (MQ=255)
                                                                                                                        gAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGt                              >  1:455828/1‑150 (MQ=255)
                                                                                                                         aaCCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATAc                                          <  2:651374/137‑1 (MQ=255)
                                                                                                                          aCCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCAGAAATCTAAACTTAGTCATGTCAAGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGTTa                            >  1:740781/1‑150 (MQ=255)
                                                                                                                              gCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCa                        <  1:1793867/150‑1 (MQ=255)
                                                                                                                              gCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCa                        >  1:1517314/1‑150 (MQ=255)
                                                                                                                               cTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCag                       >  2:174291/1‑150 (MQ=255)
                                                                                                                                 ccGTGAATGTCCTCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCagag                     <  1:1334713/150‑1 (MQ=255)
                                                                                                                                 ccGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCagag                     <  1:1401661/150‑1 (MQ=255)
                                                                                                                                 ccGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCagag                     <  1:1762766/150‑1 (MQ=255)
                                                                                                                                 ccGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGGTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCagag                     >  1:1115444/1‑150 (MQ=255)
                                                                                                                                  cGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCAGAGt                    >  2:418159/1‑150 (MQ=255)
                                                                                                                                    tGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCAGAGtta                  >  1:735322/1‑149 (MQ=255)
                                                                                                                                    tGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCAGAGTTc                  >  1:367135/1‑150 (MQ=255)
                                                                                                                                      aaatttcGCTCTGTGCCAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCAGAGTTCaa                >  1:1817791/7‑150 (MQ=255)
                                                                                                                                       aatttcGCTCTGTGCCAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCAGAGTTCaaa               >  1:1396884/6‑150 (MQ=255)
                                                                                                                                         gTCCGCTCTGGCGCAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGGTGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCAGAGTTCAAAat             >  2:620345/1‑150 (MQ=255)
                                                                                                                                         gTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCAGAGTTCAAAat             <  2:523922/150‑1 (MQ=255)
                                                                                                                                           tcGCTCTGTGCCAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCAACTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCAGAGTTAAAAattt           >  2:605047/2‑149 (MQ=255)
                                                                                                                                            cGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAACTCTAAACTTATTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCTCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCAGAGTTCAAAattga          <  1:1041386/150‑1 (MQ=255)
                                                                                                                                             gCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGTGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCAGAGTTCAAAattgat         <  1:1820738/150‑1 (MQ=255)
                                                                                                                                                ctGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGATTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCAGAGTTCAAAATTGATTGc      >  2:135471/1‑150 (MQ=255)
                                                                                                                                                  ggCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCAGAGTTCAAAATTGATTGCCg    >  1:1650723/1‑150 (MQ=255)
                                                                                                                                                    cacaGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCAGAGTTCAAAATTGATTGCCGTg  <  2:1777647/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCAGAGTTCAAAATTGATTGCCGTG  >  NC_000913/4510187‑4510484

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: