Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,519,311 A→G 5.7% intergenic (+87/+27) insB1 → / ← yjhU IS1 transposase B;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related/putative DNA‑binding transcriptional regulator; KpLE2 phage‑like element

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,519,3110AG 5.7% 373.3 / 8.3 123intergenic (+87/+27)insB1/yjhUIS1 transposase B;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related/putative DNA‑binding transcriptional regulator; KpLE2 phage‑like element
Reads supporting (aligned to +/- strand):  ref base A (48/68);  new base G (4/3);  total (52/71)
Fisher's exact test for biased strand distribution p-value = 4.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 129 total aligned reads displayed.

AAATCGGTGGAGTTGCATGACAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGCCTCTTTCCCCACCAGCAA  >  NC_000913/4519162‑4519459
                                                                                                                                                     |                                                                                                                                                    
aaaTCGGTGGAGTTGCATGACAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCata                                                                                                                                                      >  2:1502050/1‑150 (MQ=255)
aaaTCGGTGGAGTTGCATGACAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTATTATTATCGCAGCTAATCAATAAAATCCTGCACCata                                                                                                                                                      >  1:1294411/1‑150 (MQ=255)
 aaTCGGTGGAGTTGCATGACAAGGTCATCGGGCATTATCTGAAAATAAAACACTATCAGTAAGTTGGAGTCATTACCGCCCATGTTTATTTCATACATTGTGGGTATTGTTATTATAATCGCCGCTAATCAATAAAATCCTGCACCatat                                                                                                                                                     >  1:1258350/1‑150 (MQ=37)
    cGGTGGAGTTGCATGACAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTAATTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTa                                                                                                                                                  <  1:56716/150‑1 (MQ=255)
     ggTGGAGTTGCATGACAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACGATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCTATAATATCCTGCCCCATATCTaa                                                                                                                                                 >  1:180250/1‑149 (MQ=255)
       tGGAGTTGCATGACAAGGTCATCGGGCTTTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATTTTTAATTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTTCAt                                                                                                                                               <  1:1113506/150‑1 (MQ=255)
          aGTTGCATGACAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATggg                                                                                                                                            <  2:519821/150‑1 (MQ=255)
            ttGCATGACAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGc                                                                                                                                          <  1:695944/150‑1 (MQ=255)
            ttGCATGACAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGc                                                                                                                                          <  1:737594/150‑1 (MQ=255)
            ttGCATGACAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCAAATCAATAAAATCCTGCCCCATGTAGATATGGGGc                                                                                                                                          <  2:931045/150‑1 (MQ=37)
               cATGACAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATGTAGATATGGGGCAgg                                                                                                                                       <  2:1512350/150‑2 (MQ=255)
               cATGACAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAgt                                                                                                                                       >  1:1675775/1‑150 (MQ=255)
               cATGACAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCAATATCTACATGGGGCAgt                                                                                                                                       >  1:33606/1‑150 (MQ=255)
                 tGACAAGGTCATCGGGCATTATCTGAACATAAAACACTATAAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATGTAGATATGGGGCAggat                                                                                                                                     <  2:528478/150‑4 (MQ=37)
                  gACAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAgttgt                                                                                                                                    <  2:545372/150‑1 (MQ=255)
                   aCAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAgttgtt                                                                                                                                   >  1:1258014/1‑150 (MQ=255)
                   aCAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAgttgtt                                                                                                                                   >  1:1391897/1‑150 (MQ=255)
                    cAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAgttgtta                                                                                                                                  >  1:1562957/1‑149 (MQ=255)
                        gTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTc                                                                                                                              <  1:1563869/150‑1 (MQ=255)
                           aTCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCttt                                                                                                                           >  2:716369/1‑150 (MQ=255)
                            tCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCtttt                                                                                                                          <  2:1154284/150‑1 (MQ=255)
                            tCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCtttt                                                                                                                          <  2:1514684/150‑1 (MQ=255)
                            tCGGGCATTATCTGAACATAAAACACTATAAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATTCATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCtttt                                                                                                                          <  1:1445455/150‑1 (MQ=255)
                             cGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTa                                                                                                                         >  2:804875/1‑150 (MQ=255)
                                gCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAgtg                                                                                                                      <  2:1758942/150‑1 (MQ=255)
                                gCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAgtg                                                                                                                      >  1:1273181/1‑150 (MQ=255)
                                gCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGGCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAgtg                                                                                                                      <  1:1385638/150‑1 (MQ=255)
                                   ttATCTGAACATAAAACACTATCAGTAAGTTGGATTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTgg                                                                                                                   <  1:1163397/150‑1 (MQ=255)
                                   ttATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTgg                                                                                                                   <  1:922056/150‑1 (MQ=255)
                                    tATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGt                                                                                                                  <  2:1410337/150‑1 (MQ=255)
                                    tATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGt                                                                                                                  <  1:1623845/150‑1 (MQ=255)
                                         gAACATAACACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAAAAAACTCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTc                                                                                                             <  2:901041/150‑1 (MQ=255)
                                         gAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAAtta                                                                                                             >  1:1744004/1‑149 (MQ=255)
                                           aCATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCTGTTGTTCATTCTTTTAGTGTGGTAATTcac                                                                                                           >  1:1179220/1‑150 (MQ=255)
                                            cATAATACTCTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTcaca                                                                                                          <  1:716682/150‑1 (MQ=255)
                                             aTAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCacac                                                                                                         >  2:176626/1‑150 (MQ=255)
                                              tAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACg                                                                                                        >  1:1693361/1‑150 (MQ=255)
                                                aaaCACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGcc                                                                                                      >  2:1227106/1‑150 (MQ=255)
                                                 aaCACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATACTGCCAAATATCTAAATGGGGCAGTTGTTCATTCTTTTATTGTGGTAATTCACACGCCa                                                                                                     >  1:516801/1‑150 (MQ=255)
                                                     cTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCaa                                                                                                 >  2:1353851/1‑150 (MQ=255)
                                                       aTCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCAAATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACAAGCCAGCaaaa                                                                                               >  2:202828/1‑150 (MQ=255)
                                                           gTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAActc                                                                                           >  1:1044323/1‑150 (MQ=255)
                                                               tttGTAGTCATTACCGACAATGTTTATTTGTTACATTGTGGGTATTGTTCTTATTATCGCCGGTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGcc                                                                                       <  2:583782/149‑1 (MQ=255)
                                                               gTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATTGGGCAGTTTTTCATTATTTTAGTGTGGTAATTCAAACGCCAGCAAAAACTCTGcc                                                                                       >  1:244865/1‑150 (MQ=255)
                                                               gTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGcc                                                                                       <  1:414329/150‑1 (MQ=255)
                                                                   gAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTc                                                                                   <  1:1071430/150‑1 (MQ=255)
                                                                      tCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCGCATATCTACATGGGGCAGTTGTTCATTCTTATAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCtt                                                                                >  2:1664025/1‑150 (MQ=255)
                                                                        aTTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTtca                                                                              >  1:521845/1‑150 (MQ=255)
                                                                          tACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTtcatc                                                                            <  2:1675775/150‑1 (MQ=255)
                                                                          tACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTtcatc                                                                            <  2:1031129/150‑1 (MQ=255)
                                                                           aCCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTtcatca                                                                           >  2:1427292/1‑150 (MQ=255)
                                                                            ccGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATcaa                                                                          <  2:752490/150‑1 (MQ=255)
                                                                            ccGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATcaa                                                                          <  2:1653248/150‑1 (MQ=255)
                                                                              gACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCAAATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCATCAAAAACTCTGCCGTTCCTTCATcaaca                                                                        <  1:395707/150‑1 (MQ=255)
                                                                                ccATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCGCATATCTAGATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCGGCCGTTCCTTCATCAACAAt                                                                      >  1:1430120/1‑150 (MQ=255)
                                                                                 cATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATc                                                                     <  2:1391897/150‑1 (MQ=255)
                                                                                  aTGTTTATTTCATTCATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCa                                                                    <  2:1066734/150‑1 (MQ=255)
                                                                                  aTGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCa                                                                    <  1:1502050/150‑1 (MQ=255)
                                                                                  aTGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCACCATATCTACATGTGGCAGTTGTTCATTCTTTTAGTGTGTTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCa                                                                    <  1:976432/150‑1 (MQ=255)
                                                                                  aTGTTTATTTAATACATTGTGGGTATTGTTCTTATTATCTCAGCTAATCTATAAAATCCTGCCCCATATCTACATTGGGCAGTTGTTCATTCTTTTAGTGTTGTAATTCACACGCCAGCAAAAACTCTGCCGTACCTTCATCAACAATCa                                                                    <  1:291020/150‑1 (MQ=255)
                                                                                         tttCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGATcctt                                                             >  2:155068/1‑148 (MQ=255)
                                                                                          ttCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTg                                                            <  1:855442/150‑1 (MQ=255)
                                                                                          ttCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCACAATATCTAAATAGTGAAGTTTTTAATTCTTTTATTGTGGTAATACACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTg                                                            >  1:294737/1‑150 (MQ=255)
                                                                                             aTACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACa                                                         <  2:1367331/150‑1 (MQ=255)
                                                                                                    ttgGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGTGCAGTTGTTCATTCTTATATTGTGGTAATTCACACGACAGCAAAAACTCTGCCGTTCATTCATCAACAATCAGGTCCGTGACATAtcctc                                                  >  1:152395/2‑150 (MQ=255)
                                                                                                       ggTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCca                                               <  1:876777/150‑1 (MQ=255)
                                                                                                        gTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCcag                                              >  2:1116807/1‑150 (MQ=255)
                                                                                                           ttGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGACCGTGACATATCCTCCcagcag                                           <  1:948466/150‑1 (MQ=255)
                                                                                                             gTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAggg                                         >  2:1239929/1‑150 (MQ=255)
                                                                                                             gTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAggg                                         >  2:1056760/1‑150 (MQ=255)
                                                                                                              ttCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCAAATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCAGCAGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCACAGCAGGGc                                        >  1:1372399/1‑150 (MQ=255)
                                                                                                                cTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTTGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCATGTCCGTGACATATCCTCCCAGCAGGGCAc                                      <  1:1312055/150‑1 (MQ=255)
                                                                                                                cTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGGAATTCACACGCACGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGccc                                      >  1:1151625/1‑148 (MQ=255)
                                                                                                                 ttattaTCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCAcc                                     >  1:708899/1‑150 (MQ=255)
                                                                                                                  tattatCGCCGCTAATCAATAAAATCCTGCCCCATATCTTGATGGGGCAGTTGTTCATTCTTGTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCGTTCATCAACACTAAGGTCCGTGACATATCCACCCAGCAGGGCGccc                                    >  2:567764/1‑150 (MQ=255)
                                                                                                                       tCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCACCGt                               >  1:1738048/1‑150 (MQ=255)
                                                                                                                        cgccgcTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGtt                              >  1:1418469/1‑150 (MQ=255)
                                                                                                                         gccgcTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTg                             <  2:1314006/150‑1 (MQ=255)
                                                                                                                          ccgcTAATCAATAAAATCCTTCCCCATATCTACATGGGGCAGTTGGTCTTTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGc                            <  1:882874/150‑1 (MQ=255)
                                                                                                                          ccgcTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGc                            <  2:1273181/150‑1 (MQ=255)
                                                                                                                             cTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTc                         <  2:1419497/150‑1 (MQ=255)
                                                                                                                               aaTCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCAt                       >  2:568027/1‑150 (MQ=255)
                                                                                                                               aaTCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCAt                       >  1:1733023/1‑150 (MQ=255)
                                                                                                                                  cAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATGGc                    >  1:1346153/1‑150 (MQ=255)
                                                                                                                                    aTAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGCct                  >  1:1821996/1‑150 (MQ=255)
                                                                                                                                     tAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGCctc                 >  1:93969/1‑150 (MQ=255)
                                                                                                                                      aaaaTCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGCctct                >  2:323389/1‑150 (MQ=255)
                                                                                                                                      aaaaTCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGCctct                <  1:411055/150‑1 (MQ=255)
                                                                                                                                       aacaacTGCCCCATGTAGATATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCATGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGCCTCtt               >  1:1667640/6‑150 (MQ=255)
                                                                                                                                          tCCTTCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGCCTCTTTcc            <  1:967693/150‑1 (MQ=255)
                                                                                                                                          tCCTGCCCCATATCTACATGGGGCAGTTGTTCACTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGCCTCTTTcc            <  1:1352342/150‑1 (MQ=255)
                                                                                                                                           ccTGCCCCATATCTCCATGGGGCAGTTGTTCATTCTTTTAGTGTGTTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGCCTCTTTccc           <  2:1116790/150‑1 (MQ=255)
                                                                                                                                            cTGCCGCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACTATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGCCTCTTTcccc          <  2:498514/150‑1 (MQ=255)
                                                                                                                                            cTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGCCTCTTTcccc          <  2:1173049/150‑1 (MQ=255)
                                                                                                                                            cTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGCCTCTTTcccc          <  1:1376582/150‑1 (MQ=255)
                                                                                                                                            cTGCCCCAAATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGCCTCTTTcccc          <  1:276784/150‑1 (MQ=255)
                                                                                                                                                cccATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGCCTCTTTCCccacca      <  1:1036322/150‑1 (MQ=255)
                                                                                                                                                    tatCTACATGGTGCAGTTTTGCATTCTTTTAGTGTGGTATTTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGAAATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGCCTCTTTCCCCACCAGCaa  <  2:1294411/150‑1 (MQ=255)
                                                                                                                                                    tatCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAAGTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGCCTCTTTCCCCACCAGCaa  <  2:1626990/150‑1 (MQ=255)
                                                                                                                                                    tatCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATAAGGTCCGTGACATATCCTCACAGCAGGGCACACAACGTTGCGTCATTGCCTCTTTCCCCACCTGCaa  >  1:893415/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
AAATCGGTGGAGTTGCATGACAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGCCTCTTTCCCCACCAGCAA  >  NC_000913/4519162‑4519459

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: