Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,519,314 T→G 5.4% intergenic (+90/+24) insB1 → / ← yjhU IS1 transposase B;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related/putative DNA‑binding transcriptional regulator; KpLE2 phage‑like element

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,519,3140TG 5.4% 387.5 / 6.5 129intergenic (+90/+24)insB1/yjhUIS1 transposase B;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related/putative DNA‑binding transcriptional regulator; KpLE2 phage‑like element
Reads supporting (aligned to +/- strand):  ref base T (49/73);  new base G (4/3);  total (53/76)
Fisher's exact test for biased strand distribution p-value = 4.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.45e-01

Only 100 of 129 total aligned reads displayed.

CGGTGGAGTTGCATGACAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGCCTCTTTCCCCACCAGCAAGGA  >  NC_000913/4519166‑4519462
                                                                                                                                                    |                                                                                                                                                    
cGGTGGAGTTGCATGACAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTAATTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTa                                                                                                                                                     <  1:56716/150‑1 (MQ=255)
 ggTGGAGTTGCATGACAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACGATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCTATAATATCCTGCCCCATATCTaa                                                                                                                                                    >  1:180250/1‑149 (MQ=255)
   tGGAGTTGCATGACAAGGTCATCGGGCTTTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATTTTTAATTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTTCAt                                                                                                                                                  <  1:1113506/150‑1 (MQ=255)
      aGTTGCATGACAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATggg                                                                                                                                               <  2:519821/150‑1 (MQ=255)
        ttGCATGACAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGc                                                                                                                                             <  1:695944/150‑1 (MQ=255)
        ttGCATGACAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGc                                                                                                                                             <  1:737594/150‑1 (MQ=255)
        ttGCATGACAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCAAATCAATAAAATCCTGCCCCATGTAGATATGGGGc                                                                                                                                             <  2:931045/150‑1 (MQ=37)
         tGCATGACAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCa                                                                                                                                            >  1:1367331/1‑150 (MQ=255)
           cATGACAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATGTAGATATGGGGCAgg                                                                                                                                          <  2:1512350/150‑2 (MQ=255)
           cATGACAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAgt                                                                                                                                          >  1:1675775/1‑150 (MQ=255)
           cATGACAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCAATATCTACATGGGGCAgt                                                                                                                                          >  1:33606/1‑150 (MQ=255)
             tGACAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAgttg                                                                                                                                        >  1:1570020/1‑150 (MQ=255)
             tGACAAGGTCATCGGGCATTATCTGAACATAAAACACTATAAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATGTAGATATGGGGCAggat                                                                                                                                        <  2:528478/150‑4 (MQ=37)
              gACAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAgttgt                                                                                                                                       <  2:545372/150‑1 (MQ=255)
               aCAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAgttgtt                                                                                                                                      >  1:1258014/1‑150 (MQ=255)
               aCAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAgttgtt                                                                                                                                      >  1:1391897/1‑150 (MQ=255)
                cAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAgttgtta                                                                                                                                     >  1:1562957/1‑149 (MQ=255)
                    gTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTc                                                                                                                                 <  1:1563869/150‑1 (MQ=255)
                      cATCGGGCATTATCTGAACATAAAACACTATCAGAAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCtt                                                                                                                               <  1:802582/150‑1 (MQ=255)
                       aTCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCttt                                                                                                                              >  2:716369/1‑150 (MQ=255)
                       aTCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCttt                                                                                                                              <  2:1447082/150‑1 (MQ=255)
                        tCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCtttt                                                                                                                             <  2:1154284/150‑1 (MQ=255)
                        tCGGGCATTATCTGAACATAAAACACTATAAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATTCATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCtttt                                                                                                                             <  1:1445455/150‑1 (MQ=255)
                         cGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTa                                                                                                                            >  2:804875/1‑150 (MQ=255)
                          gggCATTATCTGAACATAAAACCCTATCAGTAAGCTGGAGGCCTTCCCGACCCTGTTTCATTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAg                                                                                                                           <  1:254896/150‑1 (MQ=255)
                          gggCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAg                                                                                                                           <  2:1665333/150‑1 (MQ=255)
                            gCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAgtg                                                                                                                         >  1:1273181/1‑150 (MQ=255)
                            gCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGGCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAgtg                                                                                                                         <  1:1385638/150‑1 (MQ=255)
                               ttATCTGAACATAAAACACTATCAGTAAGTTGGATTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTgg                                                                                                                      <  1:1163397/150‑1 (MQ=255)
                                tATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGt                                                                                                                     <  1:1623845/150‑1 (MQ=255)
                                tATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGt                                                                                                                     <  2:1410337/150‑1 (MQ=255)
                                     gAACATAACACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAAAAAACTCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTc                                                                                                                <  2:901041/150‑1 (MQ=255)
                                     gAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAAtta                                                                                                                >  1:1744004/1‑149 (MQ=255)
                                       aCATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCTGTTGTTCATTCTTTTAGTGTGGTAATTcac                                                                                                              >  1:1179220/1‑150 (MQ=255)
                                        cATAATACTCTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTcaca                                                                                                             <  1:716682/150‑1 (MQ=255)
                                         aTAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCacac                                                                                                            >  2:176626/1‑150 (MQ=255)
                                          tAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACg                                                                                                           >  1:1693361/1‑150 (MQ=255)
                                            aaaCACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGcc                                                                                                         >  2:1227106/1‑150 (MQ=255)
                                             aaCACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATACTGCCAAATATCTAAATGGGGCAGTTGTTCATTCTTTTATTGTGGTAATTCACACGCCa                                                                                                        >  1:516801/1‑150 (MQ=255)
                                                   aTCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCAAATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACAAGCCAGCaaaa                                                                                                  >  2:202828/1‑150 (MQ=255)
                                                       gTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAActc                                                                                              >  2:1235575/1‑150 (MQ=255)
                                                       gTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAActc                                                                                              >  1:1044323/1‑150 (MQ=255)
                                                          aGTTTGAGTCATTACCGAACATGTTTATTACATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGc                                                                                           <  2:1034029/150‑1 (MQ=255)
                                                           tttGTAGTCATTACCGACAATGTTTATTTGTTACATTGTGGGTATTGTTCTTATTATCGCCGGTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGcc                                                                                          <  2:583782/149‑1 (MQ=255)
                                                           gTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATTGGGCAGTTTTTCATTATTTTAGTGTGGTAATTCAAACGCCAGCAAAAACTCTGcc                                                                                          >  1:244865/1‑150 (MQ=255)
                                                           gTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGcc                                                                                          <  1:414329/150‑1 (MQ=255)
                                                               gAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTc                                                                                      <  1:1071430/150‑1 (MQ=255)
                                                               gAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTc                                                                                      <  1:949726/150‑1 (MQ=255)
                                                                  tCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCGCATATCTACATGGGGCAGTTGTTCATTCTTATAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCtt                                                                                   >  2:1664025/1‑150 (MQ=255)
                                                                   cATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTCTCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTtc                                                                                  <  2:1153175/150‑1 (MQ=255)
                                                                      tACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTtcatc                                                                               <  2:1031129/150‑1 (MQ=255)
                                                                      tACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTtcatc                                                                               <  2:1675775/150‑1 (MQ=255)
                                                                       aCCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTtcatca                                                                              >  2:1427292/1‑150 (MQ=255)
                                                                        ccGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATcaa                                                                             <  2:752490/150‑1 (MQ=255)
                                                                            tcATGTTTTTTTCATAAATTGTGGGTTTTGTTCTTATTATAGCGGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTATTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAAt                                                                         <  2:1306227/149‑1 (MQ=255)
                                                                             cATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATc                                                                        <  2:1391897/150‑1 (MQ=255)
                                                                              aTGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCa                                                                       <  1:1502050/150‑1 (MQ=255)
                                                                                     tttCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGATcctt                                                                >  2:155068/1‑148 (MQ=255)
                                                                                      ttCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTg                                                               <  1:855442/150‑1 (MQ=255)
                                                                                      ttCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCACAATATCTAAATAGTGAAGTTTTTAATTCTTTTATTGTGGTAATACACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTg                                                               >  1:294737/1‑150 (MQ=255)
                                                                                        cATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGAc                                                             <  2:1394623/150‑1 (MQ=255)
                                                                                         aTACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACa                                                            <  2:1367331/150‑1 (MQ=255)
                                                                                                gtgGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATAtcctc                                                     <  1:176626/150‑1 (MQ=255)
                                                                                                    gTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCcag                                                 >  2:1116807/1‑150 (MQ=255)
                                                                                                     tATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATATACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCATGTCCGTGACATATCATCCcagc                                                >  1:169063/1‑150 (MQ=255)
                                                                                                       ttGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGACCGTGACATATCCTCCcagcag                                              <  1:948466/150‑1 (MQ=255)
                                                                                                         gTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAggg                                            >  2:1056760/1‑150 (MQ=255)
                                                                                                         gTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAggg                                            >  2:1239929/1‑150 (MQ=255)
                                                                                                          ttCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCAAATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCAGCAGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCACAGCAGGGc                                           >  1:1372399/1‑150 (MQ=255)
                                                                                                           tCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGCGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCa                                          <  2:1592084/150‑1 (MQ=255)
                                                                                                            cTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTTGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCATGTCCGTGACATATCCTCCCAGCAGGGCAc                                         <  1:1312055/150‑1 (MQ=255)
                                                                                                            cTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGGAATTCACACGCACGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGccc                                         >  1:1151625/1‑148 (MQ=255)
                                                                                                              tattatCGCCGCTAATCAATAAAATCCTGCCCCATATCTTGATGGGGCAGTTGTTCATTCTTGTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCGTTCATCAACACTAAGGTCCGTGACATATCCACCCAGCAGGGCGccc                                       >  2:567764/1‑150 (MQ=255)
                                                                                                              tattatCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGGCCGTGACATATCCTCCCAGCAGGGCAccc                                       <  2:277994/150‑1 (MQ=255)
                                                                                                                   tCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCACCGt                                  >  1:1738048/1‑150 (MQ=255)
                                                                                                                    cgccgcTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGtt                                 >  1:1418469/1‑150 (MQ=255)
                                                                                                                     gccgcTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTg                                <  2:1314006/150‑1 (MQ=255)
                                                                                                                      ccgcTAATCAATAAAATCCTTCCCCATATCTACATGGGGCAGTTGGTCTTTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGc                               <  1:882874/150‑1 (MQ=255)
                                                                                                                       cgcTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCg                              <  1:312256/150‑1 (MQ=255)
                                                                                                                        gcTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGt                             <  1:1303589/150‑1 (MQ=255)
                                                                                                                        gcTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGt                             >  1:1467353/1‑150 (MQ=255)
                                                                                                                           aaTCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCAt                          >  2:568027/1‑150 (MQ=255)
                                                                                                                                 tAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGCctc                    >  1:93969/1‑150 (MQ=255)
                                                                                                                                  aaaaTCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGCctct                   >  2:323389/1‑150 (MQ=255)
                                                                                                                                  aaaaTCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGCctct                   <  1:411055/150‑1 (MQ=255)
                                                                                                                                      tCCTTCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGCCTCTTTcc               <  1:967693/150‑1 (MQ=255)
                                                                                                                                      tCCTGCCCCATATCTACATGGGGCAGTTGTTCACTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGCCTCTTTcc               <  1:1352342/150‑1 (MQ=255)
                                                                                                                                       ccTGCCCCATATCTCCATGGGGCAGTTGTTCATTCTTTTAGTGTGTTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGCCTCTTTccc              <  2:1116790/150‑1 (MQ=255)
                                                                                                                                       acTGCCCCATGTAGATATGTGGCAGTTGTTCATTATTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATAAACAATCAGGTACGACACGTATCCTCCCAGCAGGGCACCCAACGTTGCGTCAAAGCCGCTTTccc              >  1:466474/2‑150 (MQ=255)
                                                                                                                                        cTGCCGCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACTATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGCCTCTTTcccc             <  2:498514/150‑1 (MQ=255)
                                                                                                                                        cTGCCCCAAATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGCCTCTTTcccc             <  1:276784/150‑1 (MQ=255)
                                                                                                                                          gCCCCATGTAGATATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGCCATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGCCTCTTTccaaac           >  1:891509/1‑146 (MQ=255)
                                                                                                                                            cccATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGCCTCTTTCCccacca         <  1:1036322/150‑1 (MQ=255)
                                                                                                                                            cccATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGCCTCTTTCCccacca         >  1:590518/1‑150 (MQ=255)
                                                                                                                                             ccATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGCCTCTTTCCCCACCAg        <  1:1400384/150‑1 (MQ=255)
                                                                                                                                                tatCTACATGGTGCAGTTTTGCATTCTTTTAGTGTGGTATTTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGAAATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGCCTCTTTCCCCACCAGCaa     <  2:1294411/150‑1 (MQ=255)
                                                                                                                                                tatCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAAGTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGCCTCTTTCCCCACCAGCaa     <  2:1626990/150‑1 (MQ=255)
                                                                                                                                                tatCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATAAGGTCCGTGACATATCCTCACAGCAGGGCACACAACGTTGCGTCATTGCCTCTTTCCCCACCTGCaa     >  1:893415/1‑150 (MQ=255)
                                                                                                                                                  tCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGCCTCTTTCCCCACCAGCAAgg   <  2:521845/150‑1 (MQ=255)
                                                                                                                                                   cTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACAAGCCAGAAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGGGACATATCCTCCCAGCAGTGCACCCAACGTTGCGTCATAGCCTCTTTCCCCACCAGCAAgta  <  2:1519804/150‑3 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CGGTGGAGTTGCATGACAAGGTCATCGGGCATTATCTGAACATAAAACACTATCAGTAAGTTGGAGTCATTACCGACCATGTTTATTTCATACATTGTGGGTATTGTTCTTATTATCGCCGCTAATCAATAAAATCCTGCCCCATATCTACATGGGGCAGTTGTTCATTCTTTTAGTGTGGTAATTCACACGCCAGCAAAAACTCTGCCGTTCCTTCATCAACAATCAGGTCCGTGACATATCCTCCCAGCAGGGCACCCAACGTTGCGTCATAGCCTCTTTCCCCACCAGCAAGGA  >  NC_000913/4519166‑4519462

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: