BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,523,472	A→C	5.4%	F208C (TTC→TGC)	yjhG ←	putative dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,523,472	0	A	C	5.4%	481.4 / 9.1	158	F208C (TTC→TGC)	yjhG	putative dehydratase
Reads supporting (aligned to +/- strand): ref base A (80/68); new base C (4/6); total (84/74)
Fisher's exact test for biased strand distribution p-value = 5.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 159 total aligned reads displayed.

TGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCATCATGGTGGCCGGAAGCCCCTTATCGCAACTCGCC  >  NC_000913/4523327‑4523618

  aaTGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCAATGGCGaattatc                                                                                                                                              <  1:622683/150‑6 (MQ=255)

    tgtgGGATTGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAATTTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTgcg                                                                                                                                            >  2:610628/1‑150 (MQ=255)

      tgGGATTGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATTATTCGCCATTGGCGAAGCGTGCGcc                                                                                                                                          >  2:1250077/1‑150 (MQ=255)

       gggATTGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCa                                                                                                                                         >  2:1518019/1‑150 (MQ=255)

           ttGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGCACGCTTCGCCAATgg                                                                                                                                     <  2:1651416/150‑1 (MQ=255)

           ttGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGCACGCTTCGCCAATgg                                                                                                                                     <  1:1374730/150‑1 (MQ=255)

           ttGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATgg                                                                                                                                     <  1:439012/150‑1 (MQ=255)

            tGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGCACGCTTCGCCAATggc                                                                                                                                    <  1:1617177/150‑2 (MQ=255)

            tGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGCACGCTTCGCCAATggc                                                                                                                                    <  1:923029/150‑2 (MQ=255)

            tGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGt                                                                                                                                    >  1:5014/1‑150 (MQ=255)

              ccAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGCGATAATTCGCCATTGGCGCAGAGTGCGCCAATGGttt                                                                                                                                  >  2:1371576/1‑150 (MQ=255)

                 gTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGCACGCTTCGCCAATggcgaatt                                                                                                                               <  2:337267/150‑7 (MQ=255)

                  tCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGCAGCGTGCGCCAATGGTTTGCAc                                                                                                                              >  1:1638994/1‑150 (MQ=255)

                  tCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCAc                                                                                                                              <  2:1605936/150‑1 (MQ=255)

                     cAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCtt                                                                                                                           <  1:1548444/150‑1 (MQ=255)

                          cTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGt                                                                                                                      >  2:1810400/1‑150 (MQ=255)

                           ttCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGGCAATGGTTTGCACCTTGCCGtt                                                                                                                     >  2:1391617/1‑150 (MQ=255)

                            tCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTg                                                                                                                    <  2:1733255/150‑1 (MQ=255)

                             cGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGGTTGCACCTTGCCGTTGt                                                                                                                   >  2:690376/1‑150 (MQ=255)

                              ggCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGtc                                                                                                                  <  1:30021/150‑1 (MQ=255)

                              ggCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGtc                                                                                                                  <  1:1813769/150‑1 (MQ=255)

                              ggCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGtc                                                                                                                  >  2:1078788/1‑150 (MQ=255)

                               gCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGtct                                                                                                                 >  2:1134448/1‑150 (MQ=255)

                               gCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGtct                                                                                                                 <  1:1450684/150‑1 (MQ=255)

                               gCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGtct                                                                                                                 >  2:718609/1‑150 (MQ=255)

                                  accaccTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAGGCGTGCGCCAATGGTTTGCACCTTGCCGTTGtcttct                                                                                                              <  1:1286390/150‑1 (MQ=255)

                                   ccaccTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTtctc                                                                                                             <  2:589971/150‑1 (MQ=255)

                                   ccaccTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTtctc                                                                                                             <  2:1461809/150‑1 (MQ=255)

                                   ccaccTGAGATGTCCCGGCAGTGCCCAAAAATCGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTtctc                                                                                                             <  1:299981/150‑1 (MQ=255)

                                      ccTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGCTAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCAt                                                                                                          >  1:1024773/1‑150 (MQ=255)

                                                cccGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCggg                                                                                                <  1:823929/150‑1 (MQ=255)

                                                 ccGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGc                                                                                               <  1:1213306/150‑1 (MQ=255)

                                                  cGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCa                                                                                              <  2:1512232/150‑1 (MQ=255)

                                                         gCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTcgc                                                                                       >  2:893010/1‑150 (MQ=255)

                                                          cccAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTcgcg                                                                                      >  2:843360/1‑150 (MQ=255)

                                                            cAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGcgcc                                                                                    >  1:1454045/1‑150 (MQ=255)

                                                            cAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGcgcc                                                                                    >  2:804051/1‑150 (MQ=255)

                                                            cAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCAGTTGTCTTCTCCATCCTTTGCGGGCCGCGTCGcgcc                                                                                    >  1:875429/1‑150 (MQ=255)

                                                                  ttGACCGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCggg                                                                              <  1:1107281/150‑1 (MQ=255)

                                                                  ttGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCggg                                                                              <  1:283583/150‑1 (MQ=255)

                                                                  ttGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCggg                                                                              >  1:467234/1‑150 (MQ=255)

                                                                  ttGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCATTTGCGGGCAGCGTCGCGCCGCCggg                                                                              >  1:1567104/1‑150 (MQ=255)

                                                                   tGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCgggg                                                                             <  2:1278759/150‑1 (MQ=255)

                                                                     aCAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGAc                                                                           <  1:1035479/150‑1 (MQ=255)

                                                                      cAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGAcc                                                                          <  1:1245493/150‑1 (MQ=255)

                                                                      cAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGAGCCAATGGTTTGCACCTTGCCGTTGTCTTCACCATACTTTGCGGGCAGCGTCGCGCCGCCGGGGAcc                                                                          >  2:1173088/1‑150 (MQ=255)

                                                                        gccgccgccGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAg                                                                        >  1:383060/1‑150 (MQ=255)

                                                                         ccgccgccgGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCTTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGc                                                                       <  1:1432622/150‑1 (MQ=255)

                                                                         ccgccgccgGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGc                                                                       >  2:748542/1‑150 (MQ=255)

                                                                              gccgGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGt                                                                  >  1:1705876/1‑150 (MQ=255)

                                                                              gccgGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGGGCGCCAATGGGTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGt                                                                  <  2:460699/150‑1 (MQ=255)

                                                                                cgGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATACTTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTg                                                                <  2:1381022/150‑1 (MQ=255)

                                                                                cgGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTg                                                                <  2:132560/150‑1 (MQ=255)

                                                                                cgGAAGAGGCACAGGCTTTACAGCCCGCACGGCGGGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTg                                                                <  2:1171621/150‑1 (MQ=255)

                                                                                   aaGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGa                                                             >  1:985920/1‑150 (MQ=255)

                                                                                        gCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGAtgttg                                                        <  1:1134448/150‑1 (MQ=255)

                                                                                         cacaGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCCATGGTTTGCACCTTGCCGTGGTCTTCTCCATCCTTTGAGGGCCGCGTCGCGCCGCCGGGGACCCGCACGGTTGCGAtgttgt                                                       >  1:1341087/1‑150 (MQ=255)

                                                                                         cacaGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGAtgttgt                                                       >  2:1244399/1‑150 (MQ=255)

                                                                                          acaGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTtgtg                                                      >  1:1097024/1‑150 (MQ=255)

                                                                                           caGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGtgc                                                     <  1:1810400/150‑1 (MQ=255)

                                                                                                 ttACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGAGAAGCGTGCGCCAATGGTTTGCAGCTTGCCTGTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGGCGGGGAGCAGCACGGTTGCGATGTTGTGCTGcgcg                                               >  2:159240/1‑150 (MQ=255)

                                                                                                  tACAGGACGCACGCCGTGCGGGCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCgcgg                                              >  1:653260/1‑150 (MQ=255)

                                                                                                  tACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCgcgg                                              >  1:727968/1‑150 (MQ=255)

                                                                                                  tACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCgcgg                                              >  2:240082/1‑150 (MQ=255)

                                                                                                  tACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCgcgg                                              >  2:398965/1‑150 (MQ=255)

                                                                                                    cAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCgcggcg                                            <  2:1821158/150‑1 (MQ=255)

                                                                                                    cAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCgcggcg                                            <  1:1719891/150‑1 (MQ=255)

                                                                                                       cccGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCgcggcg                                            <  2:1435117/147‑1 (MQ=255)

                                                                                                        ccGCACGGCGTGCGTCCTGTAGAGATAAATCGCCATTGGCGAAGCGTGCGCCACTGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTACGCGGCGAGTg                                        <  2:281557/150‑1 (MQ=255)

                                                                                                             cGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTCCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCcatc                                   >  1:312685/1‑150 (MQ=255)

                                                                                                                   gcgtCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCATCAtggtg                             >  2:1380432/1‑150 (MQ=255)

                                                                                                                    cgtCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCATCAtggtgg                            >  1:1520391/1‑150 (MQ=255)

                                                                                                                     gtCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTTCGCGGCGAGTGCCATCATGGTGGc                           >  1:1516650/1‑150 (MQ=255)

                                                                                                                     gtCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCATCATGGTGGc                           >  1:417244/1‑150 (MQ=255)

                                                                                                                       ccTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCATCATGGTGGCCg                         <  1:384822/150‑1 (MQ=255)

                                                                                                                        cTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCATCATGGTGGCCgg                        <  2:1494221/150‑1 (MQ=255)

                                                                                                                        cTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCATCATGGTGGCCgg                        <  1:1391860/150‑1 (MQ=255)

                                                                                                                         tGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCATCATGGTGGCCGGa                       <  2:1032612/150‑1 (MQ=255)

                                                                                                                         tGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCATCATGGTGGCCGGa                       >  2:1744397/1‑150 (MQ=255)

                                                                                                                           tAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCATCATGGTGGCCGGAAg                     >  1:246472/1‑150 (MQ=255)

                                                                                                                           tAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGGTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGACCTCATGGTGGCCGGAAg                     >  1:305528/1‑150 (MQ=255)

                                                                                                                            agagATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGCTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCATCATGGTGGCCGGAAGc                    <  1:54769/150‑1 (MQ=255)

                                                                                                                               gaTAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCATCATGGTGGCCGGAAGcccc                 <  2:1022268/150‑1 (MQ=255)

                                                                                                                                aTAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCATCATGGTGGCCGGAAGCCCCt                <  1:1197927/150‑1 (MQ=255)

                                                                                                                                aTAATGCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCCTCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCATCATGGTGGCCGGAAGCCCCt                <  1:232442/150‑1 (MQ=255)

                                                                                                                                 tAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCATCATGGTGGCCGGAAGCCCCtt               >  1:1381292/1‑150 (MQ=255)

                                                                                                                                 tAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCATCATGGTGGCCGGAAGCCCCtt               <  1:738023/150‑1 (MQ=255)

                                                                                                                                 tAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCATCATGGTGGCCGGAAGCCCCtt               >  1:1154521/1‑150 (MQ=255)

                                                                                                                                         cATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCATCATGGTGGCCGGAAGCCCCTTATCGCAAc       >  2:585459/1‑150 (MQ=255)

                                                                                                                                         cATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTGGTCGTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCATCATGGTGGCCGGAAGCCCCTTATCGCAAc       <  1:724231/150‑1 (MQ=255)

                                                                                                                                          aTTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCATCATGGTGGCCGGAAGCCCCTTATCGCACCt      >  2:363222/1‑150 (MQ=255)

                                                                                                                                          aTTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCATCATGGTGGCCGGAAGCCCCTTATCGCAACt      <  1:1078788/150‑1 (MQ=255)

                                                                                                                                           ttGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCATCATGGTGGCCGGAAGCCCCTTATCGCAACTc     >  2:1253038/1‑150 (MQ=255)

                                                                                                                                            tGGCGAAGCGTGCGCCAATGGTTTGCACCTGGCCGGAGACTTCTCCCTCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCATCATGGTGGCCGGAAGCCCCTTATCGCAACTCg    <  1:355480/150‑1 (MQ=255)

                                                                                                                                             ggCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCCGCGTCGCGCAGCCGGGGACCAGCACGGTTTCGTTGTTGTGCTGCGCGGCGAGTGCCATCATGGTGGCCGGAAGCCCCTTATCCCAACTAGc   >  2:303148/1‑150 (MQ=255)

                                                                                                                                              gCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCATCATGGTGGCCGGAAGCCCCTTATCGCAACTCGcc  <  1:1309025/150‑1 (MQ=255)

                                                                                                                                              gCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCATCATGGTGGCCGGAAGCCCCTTATCGCAACTCGcc  >  2:1232179/1‑150 (MQ=255)

                                                                                                                                              gCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCATCATGGTGGCCGGAAGCCCCTTATCGCAACTCGcc  >  1:1549669/1‑150 (MQ=255)

                                                                                                                                              gCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTGCACCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCATCATGGTGGCCGGAAGCCCCTTATCGCAACTCGcc  <  1:1711414/150‑1 (MQ=255)

                                                                                                                                              gCGAAGCGTGCGCCAATGGGTTGCACCTTGCCGTTGTCATCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCATCATGGTGGCCGGAAGCCCCTTATCGCAACTCGcc  <  1:912117/150‑1 (MQ=255)

TGAATGTGGGATTGCCAGTCCCAATCCTTCGGCCACCACCTGAGATGTCCCGGCAGTGCCCAAAAATTGACAGCCGCCGCCGGAAGAGGCACAGGCTTTACAGCCCGCACGGCGTGCGTCCTGTAGAGATAATTCGCCATTGGCGAAGCGTGCGCCAATGGTTTGCACCTTGCCGTTGTCTTCTCCATCCTTTGCGGGCAGCGTCGCGCCGCCGGGGACCAGCACGGTTGCGATGTTGTGCTGCGCGGCGAGTGCCATCATGGTGGCCGGAAGCCCCTTATCGCAACTCGCC  >  NC_000913/4523327‑4523618

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`