Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,591,499 C→T 8.8% intergenic (‑220/‑158) yjiY ← / → tsr putative transporter/methyl‑accepting chemotaxis protein I, serine sensor receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,591,4990CT 8.8% 373.5 / 15.3 125intergenic (‑220/‑158)yjiY/tsrputative transporter/methyl‑accepting chemotaxis protein I, serine sensor receptor
Reads supporting (aligned to +/- strand):  ref base C (68/45);  new base T (3/8);  total (72/53)
Fisher's exact test for biased strand distribution p-value = 5.33e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 125 total aligned reads displayed.

ATTGACCGCTATCCTGTAGTGATGTTGCCAGAGTTACGCGCGCGTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTGCATCGGTCCACA  >  NC_000913/4591353‑4591643
                                                                                                                                                  |                                                                                                                                                
aTTGACCGCTATCCTGTAGTGATGTTGCCAGAGTTACGCGCGCGTAAAGTAAGGTAAATAACTGTGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTAata                                                                                                                                               >  1:596896/1‑150 (MQ=255)
aTTGACCGCTATCCTGTAGTGATGTTGCCAGAGTTACGCGCGCGTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCata                                                                                                                                               <  1:308252/150‑1 (MQ=255)
aTTGACCGCTATCCTGTAGTGATGTTGCCAGAGTTACGCGCGCGTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCata                                                                                                                                               >  2:826619/1‑150 (MQ=255)
aTTGACCGCTATCCTGTAGTGATGTTGCCAGAGTTACGCGCGCGTAAAGTAAGGTAAATAACTGAGTGGTGATTTTAGGGATGTAAGCGGGCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCata                                                                                                                                               <  1:1141711/150‑1 (MQ=255)
 ttGACCGCTATCCTGTAGTGATGTTGCCAGAGTTACGCGCGCGTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCatat                                                                                                                                              >  2:1223155/1‑150 (MQ=255)
       gCTATCCTGTAGTGATGTTGCCAGAGTTACGCGCGCGTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACata                                                                                                                                        >  2:1778631/1‑150 (MQ=255)
       gCTATCCTGTAGTGATGTTGCCAGAGTTACGCGCGCGTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACata                                                                                                                                        >  2:1516216/1‑150 (MQ=255)
         tctCCTGTAGTGATGTTGCCAGAGTTACGCGCGCGTAAAGTCAGGTAAATAACTGAGGGGTTATTTTAGGGATGTAAGCGTGCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGGTTAAGTGACGATAAATATGTGATATGAATCACATAtt                                                                                                                                      <  1:1148917/148‑1 (MQ=255)
         tATCCTGTAGTGATGTTGCCAGAGTTACGCGCGCGTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATAtt                                                                                                                                      <  1:1664005/150‑1 (MQ=255)
            ccTGTAGTGATGTTGCCAGAGTTACGCGCGCGTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTtat                                                                                                                                   <  1:1428241/150‑1 (MQ=255)
              tGTAGTGATGTTGCCAGAGTTACGCGCGCGTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACTATAAATATGTGATTCATATCACATATTTatat                                                                                                                                 >  1:277070/1‑150 (MQ=255)
              tGTAGTGATGTTGCCAGAGTTACGCGCGCGTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATATGAATCACATATTtatcg                                                                                                                                 <  1:817113/150‑3 (MQ=255)
              tGTAGTGATGTTGCCAGAGTTACGCGCGCGTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATATGAATCACATATTtatcg                                                                                                                                 <  1:1048864/150‑3 (MQ=255)
              tGTAGTGATGTTGCCAGAGTTACGCGCGCGTAAAGTAAGGTAAATAACTGAGTGGCTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATATGAATCACATATTtatcg                                                                                                                                 <  1:752439/150‑3 (MQ=255)
                  gTGATGTTGCCAGATTTACGCGCTCGTAATGTAAGGTAAATAACTGAGTGGTTATTTTACGGATGTAATCGGTGAGTTTTGCGGATGAGCGGGAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATtgtg                                                                                                                             <  2:942903/150‑1 (MQ=255)
                  gTGATGTTGCCAGAGTTACGCGCGCGTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATtgtg                                                                                                                             <  2:601860/150‑1 (MQ=255)
                   tGATGTTGCCAGAGTTACGCGCGCGTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGa                                                                                                                            <  2:1035061/150‑1 (MQ=255)
                   tGATGTTGCCAGAGTTACGCGCGCGTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATATGAATCACATATTtatcgtcact                                                                                                                            <  2:393556/150‑8 (MQ=39)
                         tGCCAGAGTTACGCGCGCGTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGAAGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAAtt                                                                                                                      >  2:739646/1‑150 (MQ=255)
                             agagTTACGCGCGCGTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCTGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATg                                                                                                                  >  2:79340/1‑150 (MQ=255)
                                gTTACGCGCGCGTAAAGTAAGGTATATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATGACATATTTATATTGTGAATAATGTATGTaa                                                                                                               >  1:307010/1‑150 (MQ=255)
                                gTTACGCGCGCGTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTaa                                                                                                               >  1:1024325/1‑150 (MQ=255)
                                    cgcgcgcgTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCTGTTGAGCGCCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATTTAaataa                                                                                                           >  2:1226824/1‑150 (MQ=255)
                                    cgcgcgcgTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAaataa                                                                                                           >  1:44378/1‑150 (MQ=255)
                                      cgcgcgTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATg                                                                                                         >  2:1599814/1‑150 (MQ=255)
                                          cgTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGAGAATAATTTATGTAAATAATGGCtt                                                                                                     >  1:1805076/1‑150 (MQ=255)
                                           gTAAAGTAAGGTAAATAACTGAGTGGTTATTTAAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAAATTAAGTAAATAATGGCttt                                                                                                    >  2:274447/1‑150 (MQ=255)
                                            tAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATAACATATTTATATTGTGAATAATTTATGTAAATAATGGCtttt                                                                                                   >  1:142504/1‑150 (MQ=255)
                                             aaaGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCCCATATTTATATTGTGAATAATTTATGTAAATAATGGCttttt                                                                                                  <  2:794158/150‑1 (MQ=255)
                                               aGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTaa                                                                                                >  1:261974/1‑150 (MQ=255)
                                                ttAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTaaa                                                                                               <  1:1372386/149‑1 (MQ=255)
                                                gTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTaaa                                                                                               >  1:1055978/1‑150 (MQ=255)
                                                gTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGAGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTaaa                                                                                               >  1:1188053/1‑150 (MQ=255)
                                                  aaGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAAtt                                                                                             <  2:395334/150‑1 (MQ=255)
                                                  aaGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAAtt                                                                                             >  2:1507837/1‑150 (MQ=255)
                                                   aGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCTGTCAGTTTTGCGGTTGAGCGGCAAATGCGTCGTTTAAGTTACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTc                                                                                            <  2:1291575/150‑1 (MQ=255)
                                                   aGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAAtta                                                                                            >  1:776820/1‑149 (MQ=255)
                                                    ggTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCa                                                                                           >  2:1346295/1‑150 (MQ=255)
                                                    ggTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCa                                                                                           >  1:1311219/1‑150 (MQ=255)
                                                     gTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTTag                                                                                          <  2:499549/150‑1 (MQ=255)
                                                       aaaTAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCagag                                                                                        >  1:531939/1‑150 (MQ=255)
                                                       aaaTAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCAAATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCagag                                                                                        >  2:39369/1‑150 (MQ=255)
                                                         aTAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAgtg                                                                                      <  1:1746515/150‑1 (MQ=255)
                                                           aaCTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGtgtg                                                                                    <  1:1517520/150‑1 (MQ=255)
                                                             cTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGaa                                                                                  >  1:190614/1‑150 (MQ=255)
                                                             cTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCAAATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGaa                                                                                  >  1:1674289/1‑150 (MQ=255)
                                                             cTGAGTGGTTATTTTAGGGATGTAAGCCGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAACTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGaa                                                                                  <  2:88617/150‑1 (MQ=255)
                                                                   ggTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAAt                                                                            <  2:1796956/150‑1 (MQ=255)
                                                                   ggTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAAt                                                                            >  2:617180/1‑150 (MQ=255)
                                                                   ggTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAAt                                                                            <  1:1484201/150‑1 (MQ=255)
                                                                       aTTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCAc                                                                        <  1:846020/150‑1 (MQ=255)
                                                                         tttAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTc                                                                      >  1:795084/1‑150 (MQ=255)
                                                                           tAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTCAAATTCAGAGTGTGAATAAAATTCACTCgg                                                                    <  1:721247/150‑1 (MQ=255)
                                                                           tAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAACGCGTCGTTTAAGTGACGATAAATATGTGATTCATAACACATATTTAAATTGTGAATAATTTATGTAAATAATTTCTTTTTAAATTCAGATACAGAATAAAAATCACTCgc                                                                    >  2:946695/1‑149 (MQ=255)
                                                                            aGGGATGTAAGCGTTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAAAAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGc                                                                   <  2:717033/150‑1 (MQ=255)
                                                                            aGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATTTTATTCAcactct                                                                   <  2:324035/150‑6 (MQ=255)
                                                                            aGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGc                                                                   <  2:1003654/150‑1 (MQ=255)
                                                                             gggATGTAAGCGGTCAGTTTTGCGGTTTAGCTGCAAAGTCGTCGTTTAAGAGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCg                                                                  <  1:339864/150‑1 (MQ=255)
                                                                                 tGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCTTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAAt                                                              <  2:707915/150‑1 (MQ=255)
                                                                                   tAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAAtct                                                            >  2:627458/1‑150 (MQ=255)
                                                                                        ggTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCcgcg                                                       >  2:1805208/1‑150 (MQ=255)
                                                                                          tCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCggg                                                     >  1:727618/1‑150 (MQ=255)
                                                                                             gTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGata                                                  >  2:1392796/1‑150 (MQ=255)
                                                                                               tttGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATAtt                                                <  1:1769404/150‑1 (MQ=255)
                                                                                                   cGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATa                                            <  1:1778631/150‑1 (MQ=255)
                                                                                                    ggTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATaa                                           >  2:495055/1‑150 (MQ=255)
                                                                                                    ggTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATaa                                           >  2:511151/1‑150 (MQ=255)
                                                                                                        tagaggCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGtt                                       <  1:1544945/146‑1 (MQ=255)
                                                                                                          gCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGtttt                                     <  2:276118/150‑1 (MQ=255)
                                                                                                          gCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGtttt                                     >  2:967575/1‑150 (MQ=255)
                                                                                                          gCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGtttt                                     >  2:1680594/1‑150 (MQ=255)
                                                                                                          gCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGtttt                                     >  2:1181116/1‑150 (MQ=255)
                                                                                                          gCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGtttt                                     <  1:1543234/150‑1 (MQ=255)
                                                                                                          gCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTAACTCGGCGTAATCTCCGCGGGATATTCATAAAGtttt                                     >  1:767059/1‑150 (MQ=255)
                                                                                                            ggCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTtttc                                   >  2:6643/1‑150 (MQ=255)
                                                                                                             gCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTtttcc                                  <  1:1005618/150‑1 (MQ=255)
                                                                                                             gCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTtttcc                                  >  1:466421/1‑150 (MQ=255)
                                                                                                             gCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTtttcc                                  >  2:260745/1‑150 (MQ=255)
                                                                                                                   gCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCa                            <  2:767055/150‑1 (MQ=255)
                                                                                                                    cgtcgtTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAg                           <  1:989579/150‑1 (MQ=255)
                                                                                                                    cgtcgtTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAg                           <  1:957299/150‑1 (MQ=255)
                                                                                                                    cgtcgtTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTACTTTCCAg                           >  2:1316791/1‑150 (MQ=255)
                                                                                                                       cgtTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGcc                        >  1:208776/1‑150 (MQ=255)
                                                                                                                            aaGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGaaaa                   >  1:1143037/1‑150 (MQ=255)
                                                                                                                             aGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAAt                  <  2:399435/150‑1 (MQ=255)
                                                                                                                               tGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCt                >  1:423526/1‑150 (MQ=255)
                                                                                                                               tGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCt                <  1:638957/150‑1 (MQ=255)
                                                                                                                               tGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCt                >  1:530433/1‑150 (MQ=255)
                                                                                                                                gACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTACAGGCCGAAAATCtt               >  1:957318/1‑150 (MQ=255)
                                                                                                                                caataTAAATATGTGATATGAATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCtt               >  1:1283295/5‑150 (MQ=255)
                                                                                                                                  cGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTGc             >  1:780122/1‑150 (MQ=255)
                                                                                                                                  cGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGGGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTGc             <  2:1171536/150‑1 (MQ=255)
                                                                                                                                    aTAAATATGTGATATGAATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTGCAt           >  2:472237/1‑150 (MQ=255)
                                                                                                                                      aaaTATGTGATATGAATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTGCATCg         >  1:911028/1‑150 (MQ=255)
                                                                                                                                       aaTATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCTCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTGCATCgg        >  1:998947/1‑150 (MQ=255)
                                                                                                                                        atatGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTGCATCGGt       >  2:1500824/1‑150 (MQ=255)
                                                                                                                                         tatGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTGCATCGGTc      <  1:1260355/150‑1 (MQ=255)
                                                                                                                                          atggtaatCATATAACATATTTATATTGTGTATAATTTATTTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAATCTTGCATCGGTCca    <  1:1501134/143‑1 (MQ=255)
                                                                                                                                          atGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTGCATCGGTcc     >  2:692605/1‑150 (MQ=255)
                                                                                                                                             tgATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTGCATCGGTCcaca  >  1:1163926/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
ATTGACCGCTATCCTGTAGTGATGTTGCCAGAGTTACGCGCGCGTAAAGTAAGGTAAATAACTGAGTGGTTATTTTAGGGATGTAAGCGGTCAGTTTTGCGGTTGAGCGGCAAAGGCGTCGTTTAAGTGACGATAAATATGTGATTCATATCACATATTTATATTGTGAATAATTTATGTAAATAATGGCTTTTTAAATTCAGAGTGTGAATAAAATTCACTCGGCGTAATCTCCGCGGGATATTCATAAAGTTTTTCCTTTCCAGGCCGAAAATCTTGCATCGGTCCACA  >  NC_000913/4591353‑4591643

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: