Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 556,018 G→A 6.0% intergenic (+22/+14) cysS → / ← ybcI cysteinyl‑tRNA synthetase/DUF457 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913556,0180GA 6.0% 324.1 / 5.8 100intergenic (+22/+14)cysS/ybcIcysteinyl‑tRNA synthetase/DUF457 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (35/59);  new base A (2/4);  total (37/63)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 103 total aligned reads displayed.

TTCAACAGCGTCTGGATGCCCGTAAAGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCAC  >  NC_000913/555869‑556166
                                                                                                                                                     |                                                                                                                                                    
ttCAACAGCGTCTGGATGCCCGTAAAGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATTGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAg                                                                                                                                                      <  1:53926/150‑1 (MQ=255)
ttCAACAGCGTCTGGATGCCCGTAAAGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGGGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAg                                                                                                                                                      <  1:214999/150‑1 (MQ=255)
 tCAACAGCGTCTGGATGCCCGTAAAGCGAAAGACTGGGCGGCGGCGGCTGCGGCGCGTCATCGTCTTAACGAGATGGGGATCGTGCTGGAAGCTGGCCCGCAAGGGACCCCCTGGCGTCGTAAGTAATTGCGCTAGTGCCGGATGCGAGt                                                                                                                                                     >  2:43609/1‑150 (MQ=255)
  cAACAGCGTCTGGCTGCCCGTAAAGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGCGtt                                                                                                                                                    >  1:1131270/1‑150 (MQ=255)
    aCAGAGTCTGGATGCCCGTAAAGCGAAAGACTGGGCGGCGGCGGAGGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATTGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTTTTGCCGGATGCGAGtttt                                                                                                                                                  <  2:1399498/150‑1 (MQ=255)
      cgCGTCTGGATGGCCGGACAGCGACAGTCTGGGCGGCGGGGGATGCGGCGCGGGATCGTCATAACGACATGGGGATCGTGCTGGAAGTTGGCCCGCATGGGTCCACCTGGCGACGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCg                                                                                                                                                <  1:358258/149‑1 (MQ=255)
           cTGGATGCCCGTAAAGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATcc                                                                                                                                           <  1:668173/150‑1 (MQ=255)
             ggATGCCCGTAAAGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAAAACTCGCATCCgg                                                                                                                                         <  2:1487814/150‑1 (MQ=255)
             ggAGGCCCGTACAGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAAAACTCGCATCCgg                                                                                                                                         <  1:1183423/150‑1 (MQ=255)
              gATGCCCGTAAAGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAAAACTCGCATCCggc                                                                                                                                        <  2:1099376/150‑2 (MQ=255)
               aTGCCCGTAAAGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCACGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAAAACTCGCATCCggca                                                                                                                                       <  2:1656329/150‑3 (MQ=255)
                tGCCCGTAAAGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTa                                                                                                                                      <  1:918430/150‑1 (MQ=255)
                       acaGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTg                                                                                                                               <  1:460909/148‑1 (MQ=255)
                       aaaGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTg                                                                                                                               <  2:715898/150‑1 (MQ=255)
                       aaaGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTg                                                                                                                               >  1:1209292/1‑150 (MQ=255)
                          gCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTgcgc                                                                                                                            >  1:1259446/1‑150 (MQ=255)
                            taaaGACTGGGCGGCGGCGGATGCGGCGCGTGCTCGTCTTAACGCGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGGCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGcca                                                                                                                          <  2:1638964/149‑1 (MQ=255)
                            gAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGcca                                                                                                                          >  2:511312/1‑150 (MQ=255)
                            gAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGcca                                                                                                                          >  1:1527770/1‑150 (MQ=255)
                             aaaGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGccac                                                                                                                         <  2:408057/150‑1 (MQ=255)
                             aaaGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGAGAGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGccac                                                                                                                         <  2:262922/150‑1 (MQ=255)
                              aaGACTGGGCGGCGGCGGATGCGGCGCGGGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGccacc                                                                                                                        <  1:790020/150‑1 (MQ=255)
                               aGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGTAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGccacca                                                                                                                       <  2:1067412/150‑1 (MQ=255)
                                gccTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTTATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTTTCGTCTGCGCcaccac                                                                                                                      >  2:299473/3‑150 (MQ=255)
                                gACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCcaccac                                                                                                                      >  2:918144/1‑150 (MQ=255)
                                  cTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCacaa                                                                                                                    <  2:519992/150‑1 (MQ=255)
                                   tGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCacaac                                                                                                                   <  2:416258/150‑1 (MQ=255)
                                     ggcggcggcGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACAt                                                                                                                 <  1:1536634/150‑1 (MQ=255)
                                        ggcggcggAGGAGGCGCGGGCTCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCTAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTGTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTcc                                                                                                              <  1:663978/150‑1 (MQ=255)
                                         gcggcggATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTccc                                                                                                             <  2:898322/150‑1 (MQ=255)
                                            gcggTTGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGTCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATAGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGGTCCTCCACAACATTCCCATc                                                                                                          <  2:270816/150‑1 (MQ=255)
                                            gcggATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATc                                                                                                          <  2:607209/150‑1 (MQ=255)
                                              ggATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAg                                                                                                        <  2:460486/150‑1 (MQ=255)
                                                   cggcgCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGCCCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCCTCAGTAGCa                                                                                                   >  2:1579996/1‑150 (MQ=255)
                                                   cggcgCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCa                                                                                                   >  2:662204/1‑150 (MQ=255)
                                                   cggcgCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCa                                                                                                   >  2:1714352/1‑150 (MQ=255)
                                                    ggcgCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACCTTCCCATCAGTAGCAt                                                                                                  >  1:331002/1‑150 (MQ=255)
                                                         gTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCg                                                                                             <  2:1527770/150‑1 (MQ=255)
                                                         gTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCAGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCg                                                                                             <  2:1184617/150‑1 (MQ=255)
                                                          tGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCgg                                                                                            >  2:154487/1‑150 (MQ=255)
                                                           gATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGc                                                                                           <  1:1267669/150‑1 (MQ=255)
                                                               gTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAAcc                                                                                       >  2:1520588/1‑150 (MQ=255)
                                                                  ttAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCCCAACATTCCCATCAGTAGCATCCCCGGCAACcaca                                                                                    >  2:569558/1‑150 (MQ=255)
                                                                   tAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCacac                                                                                   <  1:1030854/150‑1 (MQ=255)
                                                                   tAAAGAGATGGGGATCGTGCTGGAAGAAGGCCCGCTAGGGACCACCTGGCTTCGTAAGTAATTGCGCTATTGCAGGATGCTAGTTTTAGCATCCGTTTATCGTCTGCGCCACCACAACATTACCATCAGTAGCAACCCCCTCAACCagac                                                                                   >  2:878297/1‑150 (MQ=255)
                                                                    aaCGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACAcc                                                                                  <  1:54083/150‑1 (MQ=255)
                                                                          ctGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATc                                                                            <  2:1813194/149‑1 (MQ=255)
                                                                           tGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACCTTCCCATCAGTAGCATCCCCGGCAACCACCCCCCCATCa                                                                           >  1:1151956/1‑150 (MQ=255)
                                                                           tGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCa                                                                           >  1:432445/1‑150 (MQ=255)
                                                                               gATCGTTCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTc                                                                       <  1:1385044/150‑1 (MQ=255)
                                                                                aTCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCa                                                                      <  1:662204/150‑1 (MQ=255)
                                                                                aTCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCa                                                                      <  2:87002/150‑1 (MQ=255)
                                                                                 tCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAg                                                                     <  1:1665828/150‑1 (MQ=255)
                                                                                 tCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAg                                                                     <  2:1455975/150‑1 (MQ=255)
                                                                                  cGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCTGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACCTCAATTCAGa                                                                    <  1:102375/150‑1 (MQ=255)
                                                                                  cGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCACCCACACCCACATCAATTCAGa                                                                    >  1:1775001/1‑150 (MQ=255)
                                                                                  cGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGa                                                                    <  2:48454/150‑1 (MQ=255)
                                                                                    ggCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGaaa                                                                  <  2:1696499/149‑1 (MQ=255)
                                                                                     gCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGAGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAaat                                                                 <  1:1575030/150‑1 (MQ=255)
                                                                                       tGGAAGAAGGCCCGCAAGGGACCACCTGTCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCAACCACAACATTCCCCTCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAaataa                                                               >  1:352441/1‑150 (MQ=255)
                                                                                        ggAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAaataat                                                              <  2:1193438/150‑1 (MQ=255)
                                                                                        ggAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAaat                                                                 >  2:771510/1‑147 (MQ=255)
                                                                                        ggAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAaat                                                                 <  1:771510/147‑1 (MQ=255)
                                                                                           aGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCAc                                                           >  2:1145499/1‑150 (MQ=255)
                                                                                                gCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGAt                                                      >  2:1812875/1‑150 (MQ=255)
                                                                                                 cccGCAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGc                                                    >  2:618434/1‑150 (MQ=255)
                                                                                                   cGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGcc                                                   <  1:539649/150‑1 (MQ=255)
                                                                                                   cGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGcc                                                   <  2:1322588/150‑1 (MQ=255)
                                                                                                     cAATGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCg                                                 <  1:644776/150‑1 (MQ=255)
                                                                                                      aaGGGACCACCTGGCTTCGTAAGTAATTGCGCTATTGACGTATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACGACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAATTAATCACCTGATTCCCGt                                                <  1:113787/150‑1 (MQ=255)
                                                                                                       aGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTa                                               >  1:1624488/1‑150 (MQ=255)
                                                                                                         ggACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACCACCGTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCCAATAAGAAATAAACACCTGCTGCCCGGACg                                             >  1:1689575/1‑150 (MQ=255)
                                                                                                            ccaccTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCg                                          <  1:972019/150‑1 (MQ=255)
                                                                                                             caccTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCgt                                         <  2:496276/150‑1 (MQ=255)
                                                                                                              accTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCgtg                                        <  2:432445/150‑1 (MQ=255)
                                                                                                               ccTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCCCCTGATGCCCGTCCGGCgtgg                                       >  1:1291334/1‑150 (MQ=255)
                                                                                                                      tcgtAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACg                                <  2:1025008/150‑1 (MQ=255)
                                                                                                                       cgtAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAAGCACACGCACATCAATTCAGAAATACTCACCGGATGCCCGTACGGGGTGGTGTAACga                               >  1:508032/1‑150 (MQ=255)
                                                                                                                         taagtaaTTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACgaga                             >  1:6708/1‑150 (MQ=255)
                                                                                                                          aagtaaTTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGAc                            <  1:1009810/150‑1 (MQ=255)
                                                                                                                           agtaaTTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACa                           <  1:1311362/150‑1 (MQ=255)
                                                                                                                           agtaaTTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACa                           <  1:1364941/150‑1 (MQ=255)
                                                                                                                             taaTTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAAt                         <  1:171803/150‑1 (MQ=255)
                                                                                                                              aaTTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATg                        <  2:1040390/150‑1 (MQ=255)
                                                                                                                                ttGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCa                      <  2:1259446/150‑1 (MQ=255)
                                                                                                                                ttGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCa                      <  1:1486901/150‑1 (MQ=255)
                                                                                                                                  gcgcTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCAAACCCACATCAATTCAGAAATATTCACCTGATGCCCGTACGGAGTGGTGTAACGAGACAATGCaaa                    >  1:1403458/1‑150 (MQ=255)
                                                                                                                                   cgcTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAAc                   <  2:382996/150‑1 (MQ=255)
                                                                                                                                   cgcTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGAATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTTCGGCGTGGTGTTACGAGACAATGCAAAc                   >  1:428041/1‑150 (MQ=255)
                                                                                                                                     cTATTGCCGGATGCGAGTTGTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAACTAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACgg                 <  2:1717224/150‑1 (MQ=255)
                                                                                                                                      tATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTCCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACTCCCACATCAATTCAGAAATAATCACCTGATTCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGgc                >  2:895845/1‑150 (MQ=255)
                                                                                                                                        taaccGGATGCGAAAACTCGCATCCGGTTATCGTCTGCGCCCCCACAACATTCCCATCAGTAGCATCCCCGGCAACCACCCCCACATCCATTCAGAAATAATCCCCTGATGCCCGTACGGCGTGGTGTCACGCGACAATGCAAACGgcgc              >  1:1056391/4‑150 (MQ=255)
                                                                                                                                         tGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGcgcg             <  1:1248638/150‑1 (MQ=255)
                                                                                                                                          gCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCACCCCCACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGa            >  1:762281/1‑150 (MQ=255)
                                                                                                                                          accGGATGCGAAAACTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGa            >  1:1790614/2‑150 (MQ=255)
                                                                                                                                               aTGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACtttt       <  1:1294813/150‑1 (MQ=255)
                                                                                                                                                tGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTa      <  2:10858/150‑1 (MQ=255)
                                                                                                                                                  cGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCCCCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATc    >  1:613822/1‑150 (MQ=255)
                                                                                                                                                  cGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCATGGTGTAACGAGACAATGCAAACGGCGCGACTTTTAta    >  2:689332/1‑149 (MQ=255)
                                                                                                                                                    cgTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCGCCGGCAACCACACCCACATCAATTCAGAAATTATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCAc  <  2:1739426/149‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TTCAACAGCGTCTGGATGCCCGTAAAGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCAC  >  NC_000913/555869‑556166

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: