Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 556,019 T→A 6.0% intergenic (+23/+13) cysS → / ← ybcI cysteinyl‑tRNA synthetase/DUF457 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913556,0190TA 6.0% 285.0 / 4.4 98intergenic (+23/+13)cysS/ybcIcysteinyl‑tRNA synthetase/DUF457 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (33/59);  new base A (2/4);  total (35/63)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.18e-01

Only 100 of 102 total aligned reads displayed.

TCAACAGCGTCTGGATGCCCGTAAAGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACC  >  NC_000913/555870‑556167
                                                                                                                                                     |                                                                                                                                                    
tCAACAGCGTCTGGATGCCCGTAAAGCGAAAGACTGGGCGGCGGCGGCTGCGGCGCGTCATCGTCTTAACGAGATGGGGATCGTGCTGGAAGCTGGCCCGCAAGGGACCCCCTGGCGTCGTAAGTAATTGCGCTAGTGCCGGATGCGAGt                                                                                                                                                      >  2:43609/1‑150 (MQ=255)
 cAACAGCGTCTGGCTGCCCGTAAAGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGCGtt                                                                                                                                                     >  1:1131270/1‑150 (MQ=255)
   aCAGAGTCTGGATGCCCGTAAAGCGAAAGACTGGGCGGCGGCGGAGGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATTGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTTTTGCCGGATGCGAGtttt                                                                                                                                                   <  2:1399498/150‑1 (MQ=255)
     cgCGTCTGGATGGCCGGACAGCGACAGTCTGGGCGGCGGGGGATGCGGCGCGGGATCGTCATAACGACATGGGGATCGTGCTGGAAGTTGGCCCGCATGGGTCCACCTGGCGACGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCg                                                                                                                                                 <  1:358258/149‑1 (MQ=255)
          cTGGATGCCCGTAAAGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATcc                                                                                                                                            <  1:668173/150‑1 (MQ=255)
            ggATGCCCGTAAAGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAAAACTCGCATCCgg                                                                                                                                          <  2:1487814/150‑1 (MQ=255)
            ggAGGCCCGTACAGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAAAACTCGCATCCgg                                                                                                                                          <  1:1183423/150‑1 (MQ=255)
             gATGCCCGTAAAGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAAAACTCGCATCCggc                                                                                                                                         <  2:1099376/150‑2 (MQ=255)
              aTGCCCGTAAAGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCACGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAAAACTCGCATCCggca                                                                                                                                        <  2:1656329/150‑3 (MQ=255)
               tGCCCGTAAAGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTa                                                                                                                                       <  1:918430/150‑1 (MQ=255)
                      acaGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTg                                                                                                                                <  1:460909/148‑1 (MQ=255)
                      aaaGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTg                                                                                                                                >  1:1209292/1‑150 (MQ=255)
                      aaaGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTg                                                                                                                                <  2:715898/150‑1 (MQ=255)
                         gCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTgcgc                                                                                                                             >  1:1259446/1‑150 (MQ=255)
                           taaaGACTGGGCGGCGGCGGATGCGGCGCGTGCTCGTCTTAACGCGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGGCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGcca                                                                                                                           <  2:1638964/149‑1 (MQ=255)
                           gAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGcca                                                                                                                           >  2:511312/1‑150 (MQ=255)
                           gAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGcca                                                                                                                           >  1:1527770/1‑150 (MQ=255)
                            aaaGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGccac                                                                                                                          <  2:408057/150‑1 (MQ=255)
                            aaaGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGAGAGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGccac                                                                                                                          <  2:262922/150‑1 (MQ=255)
                             aaGACTGGGCGGCGGCGGATGCGGCGCGGGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGccacc                                                                                                                         <  1:790020/150‑1 (MQ=255)
                              aGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGTAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGccacca                                                                                                                        <  2:1067412/150‑1 (MQ=255)
                               gccTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTTATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTTTCGTCTGCGCcaccac                                                                                                                       >  2:299473/3‑150 (MQ=255)
                               gACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCcaccac                                                                                                                       >  2:918144/1‑150 (MQ=255)
                                 cTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCacaa                                                                                                                     <  2:519992/150‑1 (MQ=255)
                                  tGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCacaac                                                                                                                    <  2:416258/150‑1 (MQ=255)
                                    ggcggcggcGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACAt                                                                                                                  <  1:1536634/150‑1 (MQ=255)
                                       ggcggcggAGGAGGCGCGGGCTCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCTAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTGTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTcc                                                                                                               <  1:663978/150‑1 (MQ=255)
                                        gcggcggATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTccc                                                                                                              <  2:898322/150‑1 (MQ=255)
                                           gcggTTGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGTCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATAGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGGTCCTCCACAACATTCCCATc                                                                                                           <  2:270816/150‑1 (MQ=255)
                                           gcggATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATc                                                                                                           <  2:607209/150‑1 (MQ=255)
                                             ggATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAg                                                                                                         <  2:460486/150‑1 (MQ=255)
                                                  cggcgCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGCCCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCCTCAGTAGCa                                                                                                    >  2:1579996/1‑150 (MQ=255)
                                                  cggcgCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCa                                                                                                    >  2:1714352/1‑150 (MQ=255)
                                                  cggcgCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCa                                                                                                    >  2:662204/1‑150 (MQ=255)
                                                   ggcgCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACCTTCCCATCAGTAGCAt                                                                                                   >  1:331002/1‑150 (MQ=255)
                                                        gTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCg                                                                                              <  2:1527770/150‑1 (MQ=255)
                                                        gTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCAGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCg                                                                                              <  2:1184617/150‑1 (MQ=255)
                                                         tGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCgg                                                                                             >  2:154487/1‑150 (MQ=255)
                                                          gATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGc                                                                                            <  1:1267669/150‑1 (MQ=255)
                                                              gTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAAcc                                                                                        >  2:1520588/1‑150 (MQ=255)
                                                                 ttAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCCCAACATTCCCATCAGTAGCATCCCCGGCAACcaca                                                                                     >  2:569558/1‑150 (MQ=255)
                                                                  tAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCacac                                                                                    <  1:1030854/150‑1 (MQ=255)
                                                                  tAAAGAGATGGGGATCGTGCTGGAAGAAGGCCCGCTAGGGACCACCTGGCTTCGTAAGTAATTGCGCTATTGCAGGATGCTAGTTTTAGCATCCGTTTATCGTCTGCGCCACCACAACATTACCATCAGTAGCAACCCCCTCAACCagac                                                                                    >  2:878297/1‑150 (MQ=255)
                                                                   aaCGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACAcc                                                                                   <  1:54083/150‑1 (MQ=255)
                                                                         ctGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATc                                                                             <  2:1813194/149‑1 (MQ=255)
                                                                          tGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACCTTCCCATCAGTAGCATCCCCGGCAACCACCCCCCCATCa                                                                            >  1:1151956/1‑150 (MQ=255)
                                                                          tGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCa                                                                            >  1:432445/1‑150 (MQ=255)
                                                                              gATCGTTCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTc                                                                        <  1:1385044/150‑1 (MQ=255)
                                                                               aTCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCa                                                                       <  1:662204/150‑1 (MQ=255)
                                                                               aTCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCa                                                                       <  2:87002/150‑1 (MQ=255)
                                                                                tCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAg                                                                      <  2:1455975/150‑1 (MQ=255)
                                                                                tCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAg                                                                      <  1:1665828/150‑1 (MQ=255)
                                                                                 cGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCTGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACCTCAATTCAGa                                                                     <  1:102375/150‑1 (MQ=255)
                                                                                 cGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCACCCACACCCACATCAATTCAGa                                                                     >  1:1775001/1‑150 (MQ=255)
                                                                                 cGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGa                                                                     <  2:48454/150‑1 (MQ=255)
                                                                                   ggCTGGAAGATGGCCCGCAAGGTTCCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGaaa                                                                   <  2:159718/149‑1 (MQ=255)
                                                                                   ggCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGaaa                                                                   <  2:1696499/149‑1 (MQ=255)
                                                                                    gCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGAGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAaat                                                                  <  1:1575030/150‑1 (MQ=255)
                                                                                      tGGAAGAAGGCCCGCAAGGGACCACCTGTCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCAACCACAACATTCCCCTCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAaataa                                                                >  1:352441/1‑150 (MQ=255)
                                                                                       ggAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAaataat                                                               <  2:1193438/150‑1 (MQ=255)
                                                                                       ggAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAaat                                                                  >  2:771510/1‑147 (MQ=255)
                                                                                       ggAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAaat                                                                  <  1:771510/147‑1 (MQ=255)
                                                                                          aGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCAc                                                            >  2:1145499/1‑150 (MQ=255)
                                                                                               gCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGAt                                                       >  2:1812875/1‑150 (MQ=255)
                                                                                                cccGCAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGc                                                     >  2:618434/1‑150 (MQ=255)
                                                                                                 ccGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGc                                                     <  1:444743/150‑1 (MQ=255)
                                                                                                  cGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGcc                                                    <  1:539649/150‑1 (MQ=255)
                                                                                                  cGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGcc                                                    <  2:1322588/150‑1 (MQ=255)
                                                                                                    cAATGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCg                                                  <  1:644776/150‑1 (MQ=255)
                                                                                                     aaGGGACCACCTGGCTTCGTAAGTAATTGCGCTATTGACGTATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACGACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAATTAATCACCTGATTCCCGt                                                 <  1:113787/150‑1 (MQ=255)
                                                                                                      aGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTa                                                >  1:1624488/1‑150 (MQ=255)
                                                                                                        ggACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACCACCGTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCCAATAAGAAATAAACACCTGCTGCCCGGACg                                              >  1:1689575/1‑150 (MQ=255)
                                                                                                           ccaccTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCg                                           <  1:972019/150‑1 (MQ=255)
                                                                                                            caccTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCgt                                          <  2:496276/150‑1 (MQ=255)
                                                                                                             accTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCgtg                                         <  2:432445/150‑1 (MQ=255)
                                                                                                              ccTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCCCCTGATGCCCGTCCGGCgtgg                                        >  1:1291334/1‑150 (MQ=255)
                                                                                                                      cgtAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAAGCACACGCACATCAATTCAGAAATACTCACCGGATGCCCGTACGGGGTGGTGTAACga                                >  1:508032/1‑150 (MQ=255)
                                                                                                                        taagtaaTTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACgaga                              >  1:6708/1‑150 (MQ=255)
                                                                                                                         aagtaaTTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGAc                             <  1:1009810/150‑1 (MQ=255)
                                                                                                                          agtaaTTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACa                            <  1:1311362/150‑1 (MQ=255)
                                                                                                                          agtaaTTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACa                            <  1:1364941/150‑1 (MQ=255)
                                                                                                                            taaTTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAAt                          <  1:171803/150‑1 (MQ=255)
                                                                                                                             aaTTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATg                         <  2:1040390/150‑1 (MQ=255)
                                                                                                                               ttGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCa                       <  2:1259446/150‑1 (MQ=255)
                                                                                                                               ttGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCa                       <  1:1486901/150‑1 (MQ=255)
                                                                                                                                 gcgcTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCAAACCCACATCAATTCAGAAATATTCACCTGATGCCCGTACGGAGTGGTGTAACGAGACAATGCaaa                     >  1:1403458/1‑150 (MQ=255)
                                                                                                                                  cgcTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAAc                    <  2:382996/150‑1 (MQ=255)
                                                                                                                                  cgcTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGAATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTTCGGCGTGGTGTTACGAGACAATGCAAAc                    >  1:428041/1‑150 (MQ=255)
                                                                                                                                    cTATTGCCGGATGCGAGTTGTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAACTAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACgg                  <  2:1717224/150‑1 (MQ=255)
                                                                                                                                     tATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTCCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACTCCCACATCAATTCAGAAATAATCACCTGATTCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGgc                 >  2:895845/1‑150 (MQ=255)
                                                                                                                                       taaccGGATGCGAAAACTCGCATCCGGTTATCGTCTGCGCCCCCACAACATTCCCATCAGTAGCATCCCCGGCAACCACCCCCACATCCATTCAGAAATAATCCCCTGATGCCCGTACGGCGTGGTGTCACGCGACAATGCAAACGgcgc               >  1:1056391/4‑150 (MQ=255)
                                                                                                                                        tGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGcgcg              <  1:1248638/150‑1 (MQ=255)
                                                                                                                                         gCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCACCCCCACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGa             >  1:762281/1‑150 (MQ=255)
                                                                                                                                         accGGATGCGAAAACTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGa             >  1:1790614/2‑150 (MQ=255)
                                                                                                                                              aTGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACtttt        <  1:1294813/150‑1 (MQ=255)
                                                                                                                                               tGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTa       <  2:10858/150‑1 (MQ=255)
                                                                                                                                                 cGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCCCCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATc     >  1:613822/1‑150 (MQ=255)
                                                                                                                                                 cGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCATGGTGTAACGAGACAATGCAAACGGCGCGACTTTTAta     >  2:689332/1‑149 (MQ=255)
                                                                                                                                                   cgTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCGCCGGCAACCACACCCACATCAATTCAGAAATTATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCAc   <  2:1739426/149‑1 (MQ=255)
                                                                                                                                                    tttttCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCAACCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCAcc  <  2:101763/149‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TCAACAGCGTCTGGATGCCCGTAAAGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACC  >  NC_000913/555870‑556167

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: