Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 556,020 T→A 6.0% intergenic (+24/+12) cysS → / ← ybcI cysteinyl‑tRNA synthetase/DUF457 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913556,0200TA 6.0% 290.0 / 5.3 98intergenic (+24/+12)cysS/ybcIcysteinyl‑tRNA synthetase/DUF457 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (33/58);  new base A (2/4);  total (35/63)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.17e-01

Only 100 of 102 total aligned reads displayed.

CAACAGCGTCTGGATGCCCGTAAAGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTG  >  NC_000913/555871‑556169
                                                                                                                                                     |                                                                                                                                                     
cAACAGCGTCTGGCTGCCCGTAAAGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGCGtt                                                                                                                                                       >  1:1131270/1‑150 (MQ=255)
  aCAGAGTCTGGATGCCCGTAAAGCGAAAGACTGGGCGGCGGCGGAGGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATTGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTTTTGCCGGATGCGAGtttt                                                                                                                                                     <  2:1399498/150‑1 (MQ=255)
    cgCGTCTGGATGGCCGGACAGCGACAGTCTGGGCGGCGGGGGATGCGGCGCGGGATCGTCATAACGACATGGGGATCGTGCTGGAAGTTGGCCCGCATGGGTCCACCTGGCGACGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCg                                                                                                                                                   <  1:358258/149‑1 (MQ=255)
         cTGGATGCCCGTAAAGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATcc                                                                                                                                              <  1:668173/150‑1 (MQ=255)
           ggATGCCCGTAAAGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAAAACTCGCATCCgg                                                                                                                                            <  2:1487814/150‑1 (MQ=255)
           ggAGGCCCGTACAGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAAAACTCGCATCCgg                                                                                                                                            <  1:1183423/150‑1 (MQ=255)
            gATGCCCGTAAAGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAAAACTCGCATCCggc                                                                                                                                           <  2:1099376/150‑2 (MQ=255)
             aTGCCCGTAAAGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCACGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAAAACTCGCATCCggca                                                                                                                                          <  2:1656329/150‑3 (MQ=255)
              tGCCCGTAAAGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTa                                                                                                                                         <  1:918430/150‑1 (MQ=255)
                     acaGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTg                                                                                                                                  <  1:460909/148‑1 (MQ=255)
                     aaaGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTg                                                                                                                                  >  1:1209292/1‑150 (MQ=255)
                     aaaGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTg                                                                                                                                  <  2:715898/150‑1 (MQ=255)
                        gCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTgcgc                                                                                                                               >  1:1259446/1‑150 (MQ=255)
                          taaaGACTGGGCGGCGGCGGATGCGGCGCGTGCTCGTCTTAACGCGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGGCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGcca                                                                                                                             <  2:1638964/149‑1 (MQ=255)
                          gAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGcca                                                                                                                             >  2:511312/1‑150 (MQ=255)
                          gAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGcca                                                                                                                             >  1:1527770/1‑150 (MQ=255)
                           aaaGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGccac                                                                                                                            <  2:408057/150‑1 (MQ=255)
                           aaaGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGAGAGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGccac                                                                                                                            <  2:262922/150‑1 (MQ=255)
                            aaGACTGGGCGGCGGCGGATGCGGCGCGGGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGccacc                                                                                                                           <  1:790020/150‑1 (MQ=255)
                             aGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGTAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGccacca                                                                                                                          <  2:1067412/150‑1 (MQ=255)
                              gccTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTTATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTTTCGTCTGCGCcaccac                                                                                                                         >  2:299473/3‑150 (MQ=255)
                              gACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCcaccac                                                                                                                         >  2:918144/1‑150 (MQ=255)
                                cTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCacaa                                                                                                                       <  2:519992/150‑1 (MQ=255)
                                 tGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCacaac                                                                                                                      <  2:416258/150‑1 (MQ=255)
                                   ggcggcggcGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACAt                                                                                                                    <  1:1536634/150‑1 (MQ=255)
                                      ggcggcggAGGAGGCGCGGGCTCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCTAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTGTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTcc                                                                                                                 <  1:663978/150‑1 (MQ=255)
                                       gcggcggATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTccc                                                                                                                <  2:898322/150‑1 (MQ=255)
                                          gcggTTGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGTCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATAGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGGTCCTCCACAACATTCCCATc                                                                                                             <  2:270816/150‑1 (MQ=255)
                                          gcggATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATc                                                                                                             <  2:607209/150‑1 (MQ=255)
                                            ggATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAg                                                                                                           <  2:460486/150‑1 (MQ=255)
                                                 cggcgCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGCCCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCCTCAGTAGCa                                                                                                      >  2:1579996/1‑150 (MQ=255)
                                                 cggcgCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCa                                                                                                      >  2:1714352/1‑150 (MQ=255)
                                                 cggcgCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCa                                                                                                      >  2:662204/1‑150 (MQ=255)
                                                  ggcgCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACCTTCCCATCAGTAGCAt                                                                                                     >  1:331002/1‑150 (MQ=255)
                                                       gTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCg                                                                                                <  2:1527770/150‑1 (MQ=255)
                                                       gTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCAGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCg                                                                                                <  2:1184617/150‑1 (MQ=255)
                                                        tGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCgg                                                                                               >  2:154487/1‑150 (MQ=255)
                                                         gATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGc                                                                                              <  1:1267669/150‑1 (MQ=255)
                                                             gTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAAcc                                                                                          >  2:1520588/1‑150 (MQ=255)
                                                                ttAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCCCAACATTCCCATCAGTAGCATCCCCGGCAACcaca                                                                                       >  2:569558/1‑150 (MQ=255)
                                                                 tAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCacac                                                                                      <  1:1030854/150‑1 (MQ=255)
                                                                 tAAAGAGATGGGGATCGTGCTGGAAGAAGGCCCGCTAGGGACCACCTGGCTTCGTAAGTAATTGCGCTATTGCAGGATGCTAGTTTTAGCATCCGTTTATCGTCTGCGCCACCACAACATTACCATCAGTAGCAACCCCCTCAACCagac                                                                                      >  2:878297/1‑150 (MQ=255)
                                                                  aaCGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACAcc                                                                                     <  1:54083/150‑1 (MQ=255)
                                                                        ctGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATc                                                                               <  2:1813194/149‑1 (MQ=255)
                                                                         tGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACCTTCCCATCAGTAGCATCCCCGGCAACCACCCCCCCATCa                                                                              >  1:1151956/1‑150 (MQ=255)
                                                                         tGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCa                                                                              >  1:432445/1‑150 (MQ=255)
                                                                             gATCGTTCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTc                                                                          <  1:1385044/150‑1 (MQ=255)
                                                                              aTCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCa                                                                         <  2:87002/150‑1 (MQ=255)
                                                                              aTCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCa                                                                         <  1:662204/150‑1 (MQ=255)
                                                                               tCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAg                                                                        <  1:1665828/150‑1 (MQ=255)
                                                                               tCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAg                                                                        <  2:1455975/150‑1 (MQ=255)
                                                                                cGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCTGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACCTCAATTCAGa                                                                       <  1:102375/150‑1 (MQ=255)
                                                                                cGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCACCCACACCCACATCAATTCAGa                                                                       >  1:1775001/1‑150 (MQ=255)
                                                                                cGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGa                                                                       <  2:48454/150‑1 (MQ=255)
                                                                                  ggCTGGAAGATGGCCCGCAAGGTTCCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGaaa                                                                     <  2:159718/149‑1 (MQ=255)
                                                                                  ggCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGaaa                                                                     <  2:1696499/149‑1 (MQ=255)
                                                                                   gCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGAGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAaat                                                                    <  1:1575030/150‑1 (MQ=255)
                                                                                     tGGAAGAAGGCCCGCAAGGGACCACCTGTCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCAACCACAACATTCCCCTCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAaataa                                                                  >  1:352441/1‑150 (MQ=255)
                                                                                      ggAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAaataat                                                                 <  2:1193438/150‑1 (MQ=255)
                                                                                      ggAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAaat                                                                    <  1:771510/147‑1 (MQ=255)
                                                                                      ggAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAaat                                                                    >  2:771510/1‑147 (MQ=255)
                                                                                         aGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCAc                                                              >  2:1145499/1‑150 (MQ=255)
                                                                                              gCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGAt                                                         >  2:1812875/1‑150 (MQ=255)
                                                                                               cccGCAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGc                                                       >  2:618434/1‑150 (MQ=255)
                                                                                                ccGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGc                                                       <  1:444743/150‑1 (MQ=255)
                                                                                                 cGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGcc                                                      <  2:1322588/150‑1 (MQ=255)
                                                                                                 cGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGcc                                                      <  1:539649/150‑1 (MQ=255)
                                                                                                   cAATGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCg                                                    <  1:644776/150‑1 (MQ=255)
                                                                                                    aaGGGACCACCTGGCTTCGTAAGTAATTGCGCTATTGACGTATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACGACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAATTAATCACCTGATTCCCGt                                                   <  1:113787/150‑1 (MQ=255)
                                                                                                     aGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTa                                                  >  1:1624488/1‑150 (MQ=255)
                                                                                                       ggACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACCACCGTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCCAATAAGAAATAAACACCTGCTGCCCGGACg                                                >  1:1689575/1‑150 (MQ=255)
                                                                                                          ccaccTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCg                                             <  1:972019/150‑1 (MQ=255)
                                                                                                           caccTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCgt                                            <  2:496276/150‑1 (MQ=255)
                                                                                                            accTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCgtg                                           <  2:432445/150‑1 (MQ=255)
                                                                                                             ccTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCCCCTGATGCCCGTCCGGCgtgg                                          >  1:1291334/1‑150 (MQ=255)
                                                                                                                     cgtAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAAGCACACGCACATCAATTCAGAAATACTCACCGGATGCCCGTACGGGGTGGTGTAACga                                  >  1:508032/1‑150 (MQ=255)
                                                                                                                       taagtaaTTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACgaga                                >  1:6708/1‑150 (MQ=255)
                                                                                                                        aagtaaTTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGAc                               <  1:1009810/150‑1 (MQ=255)
                                                                                                                         agtaaTTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACa                              <  1:1364941/150‑1 (MQ=255)
                                                                                                                         agtaaTTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACa                              <  1:1311362/150‑1 (MQ=255)
                                                                                                                           taaTTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAAt                            <  1:171803/150‑1 (MQ=255)
                                                                                                                            aaTTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATg                           <  2:1040390/150‑1 (MQ=255)
                                                                                                                              ttGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCa                         <  1:1486901/150‑1 (MQ=255)
                                                                                                                              ttGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCa                         <  2:1259446/150‑1 (MQ=255)
                                                                                                                                gcgcTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCAAACCCACATCAATTCAGAAATATTCACCTGATGCCCGTACGGAGTGGTGTAACGAGACAATGCaaa                       >  1:1403458/1‑150 (MQ=255)
                                                                                                                                 cgcTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAAc                      <  2:382996/150‑1 (MQ=255)
                                                                                                                                 cgcTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGAATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTTCGGCGTGGTGTTACGAGACAATGCAAAc                      >  1:428041/1‑150 (MQ=255)
                                                                                                                                   cTATTGCCGGATGCGAGTTGTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAACTAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACgg                    <  2:1717224/150‑1 (MQ=255)
                                                                                                                                    tATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTCCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACTCCCACATCAATTCAGAAATAATCACCTGATTCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGgc                   >  2:895845/1‑150 (MQ=255)
                                                                                                                                      taaccGGATGCGAAAACTCGCATCCGGTTATCGTCTGCGCCCCCACAACATTCCCATCAGTAGCATCCCCGGCAACCACCCCCACATCCATTCAGAAATAATCCCCTGATGCCCGTACGGCGTGGTGTCACGCGACAATGCAAACGgcgc                 >  1:1056391/4‑150 (MQ=255)
                                                                                                                                       tGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGcgcg                <  1:1248638/150‑1 (MQ=255)
                                                                                                                                        gCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCACCCCCACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGa               >  1:762281/1‑150 (MQ=255)
                                                                                                                                        accGGATGCGAAAACTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGa               >  1:1790614/2‑150 (MQ=255)
                                                                                                                                             aTGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACtttt          <  1:1294813/150‑1 (MQ=255)
                                                                                                                                              tGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTa         <  2:10858/150‑1 (MQ=255)
                                                                                                                                                cGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCCCCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATc       >  1:613822/1‑150 (MQ=255)
                                                                                                                                                cGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCATGGTGTAACGAGACAATGCAAACGGCGCGACTTTTAta       >  2:689332/1‑149 (MQ=255)
                                                                                                                                                  cgTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCGCCGGCAACCACACCCACATCAATTCAGAAATTATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCAc     <  2:1739426/149‑1 (MQ=255)
                                                                                                                                                   tttttCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCAACCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCAcc    <  2:101763/149‑1 (MQ=255)
                                                                                                                                                     tttCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTg  <  2:1200697/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CAACAGCGTCTGGATGCCCGTAAAGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTG  >  NC_000913/555871‑556169

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: