Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 556,021 T→C 6.1% intergenic (+25/+11) cysS → / ← ybcI cysteinyl‑tRNA synthetase/DUF457 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913556,0210TC 6.1% 293.0 / 7.4 98intergenic (+25/+11)cysS/ybcIcysteinyl‑tRNA synthetase/DUF457 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (33/58);  new base C (2/4);  total (35/63)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.75e-01

Only 100 of 101 total aligned reads displayed.

ACAGCGTCTGGATGCCCGTAAAGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTG  >  NC_000913/555873‑556169
                                                                                                                                                    |                                                                                                                                                    
aCAGAGTCTGGATGCCCGTAAAGCGAAAGACTGGGCGGCGGCGGAGGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATTGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTTTTGCCGGATGCGAGtttt                                                                                                                                                     <  2:1399498/150‑1 (MQ=255)
  cgCGTCTGGATGGCCGGACAGCGACAGTCTGGGCGGCGGGGGATGCGGCGCGGGATCGTCATAACGACATGGGGATCGTGCTGGAAGTTGGCCCGCATGGGTCCACCTGGCGACGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCg                                                                                                                                                   <  1:358258/149‑1 (MQ=255)
       cTGGATGCCCGTAAAGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATcc                                                                                                                                              <  1:668173/150‑1 (MQ=255)
         ggATGCCCGTAAAGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAAAACTCGCATCCgg                                                                                                                                            <  2:1487814/150‑1 (MQ=255)
         ggAGGCCCGTACAGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAAAACTCGCATCCgg                                                                                                                                            <  1:1183423/150‑1 (MQ=255)
          gATGCCCGTAAAGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAAAACTCGCATCCggc                                                                                                                                           <  2:1099376/150‑2 (MQ=255)
           aTGCCCGTAAAGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCACGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAAAACTCGCATCCggca                                                                                                                                          <  2:1656329/150‑3 (MQ=255)
            tGCCCGTAAAGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTa                                                                                                                                         <  1:918430/150‑1 (MQ=255)
                   acaGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTg                                                                                                                                  <  1:460909/148‑1 (MQ=255)
                   aaaGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTg                                                                                                                                  >  1:1209292/1‑150 (MQ=255)
                   aaaGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTg                                                                                                                                  <  2:715898/150‑1 (MQ=255)
                      gCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTgcgc                                                                                                                               >  1:1259446/1‑150 (MQ=255)
                        taaaGACTGGGCGGCGGCGGATGCGGCGCGTGCTCGTCTTAACGCGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGGCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGcca                                                                                                                             <  2:1638964/149‑1 (MQ=255)
                        gAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGcca                                                                                                                             >  1:1527770/1‑150 (MQ=255)
                        gAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGcca                                                                                                                             >  2:511312/1‑150 (MQ=255)
                         aaaGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGccac                                                                                                                            <  2:408057/150‑1 (MQ=255)
                         aaaGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGAGAGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGccac                                                                                                                            <  2:262922/150‑1 (MQ=255)
                          aaGACTGGGCGGCGGCGGATGCGGCGCGGGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGccacc                                                                                                                           <  1:790020/150‑1 (MQ=255)
                           aGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGTAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGccacca                                                                                                                          <  2:1067412/150‑1 (MQ=255)
                            gccTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTTATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTTTCGTCTGCGCcaccac                                                                                                                         >  2:299473/3‑150 (MQ=255)
                            gACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCcaccac                                                                                                                         >  2:918144/1‑150 (MQ=255)
                              cTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCacaa                                                                                                                       <  2:519992/150‑1 (MQ=255)
                               tGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCacaac                                                                                                                      <  2:416258/150‑1 (MQ=255)
                                 ggcggcggcGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACAt                                                                                                                    <  1:1536634/150‑1 (MQ=255)
                                    ggcggcggAGGAGGCGCGGGCTCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCTAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTGTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTcc                                                                                                                 <  1:663978/150‑1 (MQ=255)
                                     gcggcggATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTccc                                                                                                                <  2:898322/150‑1 (MQ=255)
                                        gcggTTGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGTCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATAGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGGTCCTCCACAACATTCCCATc                                                                                                             <  2:270816/150‑1 (MQ=255)
                                        gcggATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATc                                                                                                             <  2:607209/150‑1 (MQ=255)
                                          ggATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAg                                                                                                           <  2:460486/150‑1 (MQ=255)
                                               cggcgCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGCCCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCCTCAGTAGCa                                                                                                      >  2:1579996/1‑150 (MQ=255)
                                               cggcgCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCa                                                                                                      >  2:1714352/1‑150 (MQ=255)
                                               cggcgCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCa                                                                                                      >  2:662204/1‑150 (MQ=255)
                                                ggcgCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACCTTCCCATCAGTAGCAt                                                                                                     >  1:331002/1‑150 (MQ=255)
                                                     gTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCg                                                                                                <  2:1527770/150‑1 (MQ=255)
                                                     gTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCAGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCg                                                                                                <  2:1184617/150‑1 (MQ=255)
                                                      tGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCgg                                                                                               >  2:154487/1‑150 (MQ=255)
                                                       gATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGc                                                                                              <  1:1267669/150‑1 (MQ=255)
                                                           gTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAAcc                                                                                          >  2:1520588/1‑150 (MQ=255)
                                                              ttAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCCCAACATTCCCATCAGTAGCATCCCCGGCAACcaca                                                                                       >  2:569558/1‑150 (MQ=255)
                                                               tAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCacac                                                                                      <  1:1030854/150‑1 (MQ=255)
                                                               tAAAGAGATGGGGATCGTGCTGGAAGAAGGCCCGCTAGGGACCACCTGGCTTCGTAAGTAATTGCGCTATTGCAGGATGCTAGTTTTAGCATCCGTTTATCGTCTGCGCCACCACAACATTACCATCAGTAGCAACCCCCTCAACCagac                                                                                      >  2:878297/1‑150 (MQ=255)
                                                                aaCGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACAcc                                                                                     <  1:54083/150‑1 (MQ=255)
                                                                      ctGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATc                                                                               <  2:1813194/149‑1 (MQ=255)
                                                                       tGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACCTTCCCATCAGTAGCATCCCCGGCAACCACCCCCCCATCa                                                                              >  1:1151956/1‑150 (MQ=255)
                                                                       tGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCa                                                                              >  1:432445/1‑150 (MQ=255)
                                                                           gATCGTTCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTc                                                                          <  1:1385044/150‑1 (MQ=255)
                                                                            aTCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCa                                                                         <  2:87002/150‑1 (MQ=255)
                                                                            aTCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCa                                                                         <  1:662204/150‑1 (MQ=255)
                                                                             tCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAg                                                                        <  1:1665828/150‑1 (MQ=255)
                                                                             tCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAg                                                                        <  2:1455975/150‑1 (MQ=255)
                                                                              cGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCTGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACCTCAATTCAGa                                                                       <  1:102375/150‑1 (MQ=255)
                                                                              cGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCACCCACACCCACATCAATTCAGa                                                                       >  1:1775001/1‑150 (MQ=255)
                                                                              cGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGa                                                                       <  2:48454/150‑1 (MQ=255)
                                                                                ggCTGGAAGATGGCCCGCAAGGTTCCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGaaa                                                                     <  2:159718/149‑1 (MQ=255)
                                                                                ggCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGaaa                                                                     <  2:1696499/149‑1 (MQ=255)
                                                                                 gCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGAGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCTCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAaat                                                                    <  1:1575030/150‑1 (MQ=255)
                                                                                   tGGAAGAAGGCCCGCAAGGGACCACCTGTCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCAACCACAACATTCCCCTCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAaataa                                                                  >  1:352441/1‑150 (MQ=255)
                                                                                    ggAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAaataat                                                                 <  2:1193438/150‑1 (MQ=255)
                                                                                    ggAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAaat                                                                    >  2:771510/1‑147 (MQ=255)
                                                                                    ggAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAaat                                                                    <  1:771510/147‑1 (MQ=255)
                                                                                       aGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCAc                                                              >  2:1145499/1‑150 (MQ=255)
                                                                                            gCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGAt                                                         >  2:1812875/1‑150 (MQ=255)
                                                                                             cccGCAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGc                                                       >  2:618434/1‑150 (MQ=255)
                                                                                              ccGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGc                                                       <  1:444743/150‑1 (MQ=255)
                                                                                               cGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGcc                                                      <  2:1322588/150‑1 (MQ=255)
                                                                                               cGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGcc                                                      <  1:539649/150‑1 (MQ=255)
                                                                                                 cAATGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCg                                                    <  1:644776/150‑1 (MQ=255)
                                                                                                  aaGGGACCACCTGGCTTCGTAAGTAATTGCGCTATTGACGTATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACGACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAATTAATCACCTGATTCCCGt                                                   <  1:113787/150‑1 (MQ=255)
                                                                                                   aGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTa                                                  >  1:1624488/1‑150 (MQ=255)
                                                                                                     ggACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACCACCGTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCCAATAAGAAATAAACACCTGCTGCCCGGACg                                                >  1:1689575/1‑150 (MQ=255)
                                                                                                        ccaccTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCg                                             <  1:972019/150‑1 (MQ=255)
                                                                                                         caccTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCgt                                            <  2:496276/150‑1 (MQ=255)
                                                                                                          accTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCgtg                                           <  2:432445/150‑1 (MQ=255)
                                                                                                           ccTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCCCCTGATGCCCGTCCGGCgtgg                                          >  1:1291334/1‑150 (MQ=255)
                                                                                                                  tcgtAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACg                                   <  2:1025008/150‑1 (MQ=255)
                                                                                                                   cgtAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAAGCACACGCACATCAATTCAGAAATACTCACCGGATGCCCGTACGGGGTGGTGTAACga                                  >  1:508032/1‑150 (MQ=255)
                                                                                                                     taagtaaTTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACgaga                                >  1:6708/1‑150 (MQ=255)
                                                                                                                      aagtaaTTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGAc                               <  1:1009810/150‑1 (MQ=255)
                                                                                                                       agtaaTTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACa                              <  1:1311362/150‑1 (MQ=255)
                                                                                                                       agtaaTTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACa                              <  1:1364941/150‑1 (MQ=255)
                                                                                                                         taaTTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAAt                            <  1:171803/150‑1 (MQ=255)
                                                                                                                          aaTTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATg                           <  2:1040390/150‑1 (MQ=255)
                                                                                                                            ttGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCa                         <  2:1259446/150‑1 (MQ=255)
                                                                                                                            ttGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCa                         <  1:1486901/150‑1 (MQ=255)
                                                                                                                              gcgcTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCAAACCCACATCAATTCAGAAATATTCACCTGATGCCCGTACGGAGTGGTGTAACGAGACAATGCaaa                       >  1:1403458/1‑150 (MQ=255)
                                                                                                                               cgcTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAAc                      <  2:382996/150‑1 (MQ=255)
                                                                                                                               cgcTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGAATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTTCGGCGTGGTGTTACGAGACAATGCAAAc                      >  1:428041/1‑150 (MQ=255)
                                                                                                                                 cTATTGCCGGATGCGAGTTGTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAACTAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACgg                    <  2:1717224/150‑1 (MQ=255)
                                                                                                                                  tATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTCCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACTCCCACATCAATTCAGAAATAATCACCTGATTCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGgc                   >  2:895845/1‑150 (MQ=255)
                                                                                                                                    taaccGGATGCGAAAACTCGCATCCGGTTATCGTCTGCGCCCCCACAACATTCCCATCAGTAGCATCCCCGGCAACCACCCCCACATCCATTCAGAAATAATCCCCTGATGCCCGTACGGCGTGGTGTCACGCGACAATGCAAACGgcgc                 >  1:1056391/4‑150 (MQ=255)
                                                                                                                                     tGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGcgcg                <  1:1248638/150‑1 (MQ=255)
                                                                                                                                      gCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCACCCCCACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGa               >  1:762281/1‑150 (MQ=255)
                                                                                                                                      accGGATGCGAAAACTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGa               >  1:1790614/2‑150 (MQ=255)
                                                                                                                                           aTGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACtttt          <  1:1294813/150‑1 (MQ=255)
                                                                                                                                            tGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTa         <  2:10858/150‑1 (MQ=255)
                                                                                                                                              cGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCCCCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATc       >  1:613822/1‑150 (MQ=255)
                                                                                                                                              cGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCATGGTGTAACGAGACAATGCAAACGGCGCGACTTTTAta       >  2:689332/1‑149 (MQ=255)
                                                                                                                                                cgTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCGCCGGCAACCACACCCACATCAATTCAGAAATTATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCAc     <  2:1739426/149‑1 (MQ=255)
                                                                                                                                                 tttttCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCAACCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCAcc    <  2:101763/149‑1 (MQ=255)
                                                                                                                                                   tttCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTg  <  2:1200697/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
ACAGCGTCTGGATGCCCGTAAAGCGAAAGACTGGGCGGCGGCGGATGCGGCGCGTGATCGTCTTAACGAGATGGGGATCGTGCTGGAAGATGGCCCGCAAGGGACCACCTGGCGTCGTAAGTAATTGCGCTATTGCCGGATGCGAGTTTTCGCATCCGGTTATCGTCTGCGCCACCACAACATTCCCATCAGTAGCATCCCCGGCAACCACACCCACATCAATTCAGAAATAATCACCTGATGCCCGTACGGCGTGGTGTAACGAGACAATGCAAACGGCGCGACTTTTATCACCTG  >  NC_000913/555873‑556169

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: