Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 556,642 A→C 6.0% intergenic (‑89/+19) ybcI ← / ← ybcJ DUF457 family inner membrane protein/ribosome‑associated protein; putative RNA‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913556,6420AC 6.0% 291.1 / 5.5 106intergenic (‑89/+19)ybcI/ybcJDUF457 family inner membrane protein/ribosome‑associated protein; putative RNA‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (46/53);  new base C (5/2);  total (51/55)
Fisher's exact test for biased strand distribution p-value = 2.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 107 total aligned reads displayed.

TCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTGC  >  NC_000913/556493‑556790
                                                                                                                                                     |                                                                                                                                                    
tCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCTTAGAGGGCAAAAGTATGAAAAGAGAACCGGCCTGTTGa                                                                                                                                                      >  2:1689670/1‑150 (MQ=255)
 cGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGAt                                                                                                                                                     >  2:991566/1‑150 (MQ=255)
 cGAACACAACCCTAAACCAATGCATAGGGGAAAGGCGGCTTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATTCTATAAGCATGCATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGGTGAt                                                                                                                                                     >  2:1612763/1‑150 (MQ=255)
   aaaccaacccTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCTATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATAc                                                                                                                                                   <  1:1275518/147‑1 (MQ=255)
     cccaacccTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAg                                                                                                                                                 >  2:531484/1‑150 (MQ=255)
     cccaacccTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAg                                                                                                                                                 >  2:436832/1‑150 (MQ=255)
       caacccTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCAATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGc                                                                                                                                               >  2:33243/1‑150 (MQ=255)
        aacccTACACCCATGCATCGGGGACCGGCGGCGTGCGTAATACCGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGAGGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGcc                                                                                                                                              <  2:704652/150‑1 (MQ=255)
          cccTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCgg                                                                                                                                            >  2:414832/1‑150 (MQ=255)
          cccTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTATCAACAGGCCgg                                                                                                                                            <  1:1658812/150‑1 (MQ=255)
          cccTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGGCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCgg                                                                                                                                            <  1:964629/150‑1 (MQ=255)
          cccTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGGTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGCGGGCAAAAGTCTGCAAAGAGACCCGGCCTGGTGATACAGGCCgg                                                                                                                                            >  1:321725/1‑150 (MQ=255)
          cccTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGGTGGCATGATGGGCGCTTCGGCAAAATGTCGATGCAATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCgg                                                                                                                                            <  1:767205/150‑1 (MQ=255)
           ccTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCggg                                                                                                                                           <  1:446204/150‑1 (MQ=255)
           ccTAAACCAAAGCATAGGGGAACGGCGGCGTGCGTAATAACGGGTGGCAGGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCAGGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCggg                                                                                                                                           <  1:333242/150‑1 (MQ=255)
            cTAATCCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTAGCAGCATGGATGAACGGGGCGGAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGa                                                                                                                                          <  1:773939/150‑1 (MQ=255)
            cTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGa                                                                                                                                          >  1:385299/1‑150 (MQ=255)
                aCCAATGCATAGGGGAACGGCGGCGTGCGTAATATCGGTTGGCATGAGGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGATCCGGCCTGTTGATACAGGCCGGGAAAgg                                                                                                                                      <  2:1345067/150‑1 (MQ=255)
                    aTGCATAGGGGAACGGCGGCGTTCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATc                                                                                                                                  <  1:1736667/150‑1 (MQ=255)
                    aTGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATc                                                                                                                                  <  2:610547/150‑1 (MQ=255)
                     tGCATCGGGGACCGGCGGCGTGCGTAATATCGGTTGGCATGAAGGCCGCTTCGGCAAAATGTCGATCCTATCAGCATGGATGAACGGTGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCa                                                                                                                                 <  2:1577169/150‑1 (MQ=255)
                      tcATAGTGGCCCGGCGGCGTGAGTAATAACGGTTGGCATGATGGTCGCTTCCGCAAAATCTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAACGTCTGCACAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAg                                                                                                                                <  1:163767/149‑1 (MQ=255)
                      gCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAg                                                                                                                                <  2:373436/150‑1 (MQ=255)
                       cATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAgg                                                                                                                               >  2:439086/1‑150 (MQ=255)
                       cATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCGATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAgg                                                                                                                               <  1:970823/150‑1 (MQ=255)
                        aTAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGc                                                                                                                              <  2:1090790/150‑1 (MQ=255)
                         tAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGca                                                                                                                             <  1:1273716/150‑1 (MQ=255)
                         tAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGca                                                                                                                             <  2:1563131/150‑1 (MQ=255)
                         tAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGca                                                                                                                             >  2:1205720/1‑150 (MQ=255)
                          aGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGcaa                                                                                                                            >  2:1748035/1‑150 (MQ=255)
                                    cggcgTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTAc                                                                                                                  >  1:469340/1‑150 (MQ=255)
                                     ggcgTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACg                                                                                                                 <  2:1525419/150‑1 (MQ=255)
                                      gcgtgcgtAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGGATGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGc                                                                                                                <  2:183918/150‑1 (MQ=255)
                                       cgtgcgtAATACCGGATGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCt                                                                                                               <  2:307489/150‑1 (MQ=255)
                                          gcgtAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCtgtg                                                                                                            <  2:712040/150‑1 (MQ=255)
                                          gcgtAATAACGGTCGGCATGATGGGCGCTTCGGCAAAATGTCGATGCTAGCAGCATGGATGAACGGGTCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCtgtg                                                                                                            <  2:928908/150‑1 (MQ=255)
                                             taataaCGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCCGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGAcc                                                                                                         >  2:121667/1‑150 (MQ=255)
                                               ataaCGGTGGGCATGCTGGTCGCTTCGGCAAAATGTCGATGCTATCAGCAGGGATGAACGGCGCGTAGAGGGCAAAAGTCTGAACAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTg                                                                                                       <  2:1439915/150‑1 (MQ=255)
                                                 aaCGTTTGGCATTAGGGTCGCTTCGGCAAAATGTCGCTGCTATCAGCATGGAGGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACCGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCa                                                                                                     <  2:303412/150‑1 (MQ=255)
                                                 aaCGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCa                                                                                                     <  2:856970/150‑1 (MQ=255)
                                                  aCGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCaa                                                                                                    >  2:1253351/1‑150 (MQ=255)
                                                    ggTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTACAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCaaca                                                                                                  >  1:705527/1‑148 (MQ=255)
                                                       tGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACta                                                                                               >  2:969946/1‑149 (MQ=255)
                                                       tGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTc                                                                                               <  1:1193091/150‑1 (MQ=255)
                                                         gCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCaa                                                                                             >  2:1753061/1‑149 (MQ=255)
                                                         gCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCAc                                                                                             >  2:1416842/1‑150 (MQ=255)
                                                         gCATGATGGTCGCTTCGGCAAAAGGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCTGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACACCCTGTACGCTGTGACCTGCAAAACTCAc                                                                                             >  2:245811/1‑150 (MQ=255)
                                                               tgGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCtn                                                                                       <  2:449036/150‑2 (MQ=255)
                                                                    gCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCgg                                                                                  >  1:947718/1‑150 (MQ=255)
                                                                        cGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGcgac                                                                              >  1:1031228/1‑150 (MQ=255)
                                                                            aaaaTGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGAAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGCCCGGCGACGAtt                                                                          >  1:333573/1‑150 (MQ=255)
                                                                                  tCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAg                                                                    <  2:679885/150‑1 (MQ=255)
                                                                                       gCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGCCCTGCAAAACTCACTGTCAGACCGGCGCCGATTTTGCAGCGttt                                                               >  2:1155399/1‑150 (MQ=255)
                                                                                       gCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGttt                                                               >  1:588132/1‑150 (MQ=255)
                                                                                         tATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCGGTACGCTGTGACATGCAAAACGCAATGTCTGACCGGAGACGATTTTGCAGCGttttc                                                             >  2:1090525/1‑148 (MQ=255)
                                                                                         tATCAGCAGTGCGGAACGGGGCGTAGAGGGCAAAAGTCGGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTgc                                                             <  1:1509877/150‑1 (MQ=255)
                                                                                           tCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTgcgc                                                           >  1:682622/1‑150 (MQ=255)
                                                                                              gCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGtt                                                        >  1:1757041/1‑150 (MQ=255)
                                                                                              gCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGtt                                                        >  2:619604/1‑150 (MQ=255)
                                                                                              gCATGGATGAACGGGGAGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGtt                                                        >  2:626891/1‑150 (MQ=255)
                                                                                               cATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGttt                                                       <  2:385299/150‑1 (MQ=255)
                                                                                               cATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGttt                                                       <  1:616206/150‑1 (MQ=255)
                                                                                               cATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGttt                                                       >  2:1251288/1‑150 (MQ=255)
                                                                                                aTGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTc                                                      <  2:1740297/150‑1 (MQ=255)
                                                                                                 tGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGATAACCGCCCTGTTGATACAGTCCGGGAAAGGGATCAGGCAACAACATGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCa                                                     <  2:861258/150‑1 (MQ=255)
                                                                                                  ggATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCaa                                                    >  2:485262/1‑150 (MQ=255)
                                                                                                   gATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAAc                                                   >  2:1594013/1‑150 (MQ=255)
                                                                                                    aTGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAAcc                                                  <  1:1748035/150‑1 (MQ=255)
                                                                                                       aaCGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGATAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAAGTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCa                                               >  2:345158/1‑150 (MQ=255)
                                                                                                           gggCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGt                                           >  2:1005258/1‑150 (MQ=255)
                                                                                                               gTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGAc                                       <  1:1253351/150‑1 (MQ=255)
                                                                                                                 cgagGGCACAAGTCTGACAAGAGCACCGGCCTGTGGATACAGGCCGGGAAAGGGCTCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGGCTGACCGGCGACGATGTTGCAGCGGGTGCGCGTTTCAACCGCACCGTCGACtt                                     <  1:859723/149‑1 (MQ=255)
                                                                                                                  gcgggCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGTGATCCGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACttt                                    <  2:1380097/148‑1 (MQ=255)
                                                                                                                  gagGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACAGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACAGTCGACttt                                    >  2:63406/1‑150 (MQ=255)
                                                                                                                   agGGCAAAAGTCAGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCCCTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCATCCGCACCGGCGACTTTc                                   >  1:311619/1‑150 (MQ=255)
                                                                                                                     ggCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCAc                                 <  1:991566/150‑1 (MQ=255)
                                                                                                                      gCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAACCTCACTGTCGGCCCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTcccc                                >  2:1503777/1‑150 (MQ=255)
                                                                                                                       cAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGCCCGGCGACGATTTTGCTGCGTTTGCGCGGTTCCAGCGCCCCGTCGGCTTTCACCt                               >  1:728749/1‑150 (MQ=255)
                                                                                                                              cTGATAAGAGATCCGGCCTGTTTATACAGGACGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTc                        <  2:1213792/150‑1 (MQ=255)
                                                                                                                              cTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTc                        <  2:1791655/150‑1 (MQ=255)
                                                                                                                               tGAAAAGAGAACCGGGCTGTTGCTACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCg                       <  1:43020/150‑1 (MQ=255)
                                                                                                                               tGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGAGTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCg                       >  2:1547256/1‑150 (MQ=255)
                                                                                                                                gAAAAGAGAACCGGCCTGTTGATTCAGGCCGGGAAAGGGATCAGGCCACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTGGGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCgg                      <  1:273032/150‑1 (MQ=255)
                                                                                                                                gAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCgg                      <  2:82566/150‑1 (MQ=255)
                                                                                                                                gAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCgg                      >  2:1557366/1‑150 (MQ=255)
                                                                                                                                 aaaaGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGc                     <  1:1205720/150‑1 (MQ=255)
                                                                                                                                 aaaaGAGAACCGGCCTGTGGATACAGGCCGGGAAAGGGAGCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGc                     <  2:1741333/150‑1 (MQ=255)
                                                                                                                                  aaaGTGAACCGGCCTGTTGATACAGGCCGGGAAAGTGATCAGGCAACAACGTGTACGCTGTGACCTGCAAAACTCACTGTCTGAGCGGCGACGATTTTGCAGCGTTTGCGCGTTTCATCCGCACCGTCGACTTTCACCTGGCCTTCGGCa                    <  2:128041/150‑1 (MQ=255)
                                                                                                                                    agagAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAACCTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCCCCGTCGACTTTCACCTGGCCTTCGGCact                  >  1:663803/1‑148 (MQ=255)
                                                                                                                                        aaCCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATcgcg              <  2:669702/150‑1 (MQ=255)
                                                                                                                                         cccGGCCTGTATCAACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGa             >  1:566438/2‑150 (MQ=255)
                                                                                                                                         cccGGCCTGTATCAACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGa             >  2:1423441/2‑150 (MQ=255)
                                                                                                                                          ccGGCCTGTATCAACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACAGTCGACTTTCACCTGGCCTTCGGCAATCGCGAt            >  2:26419/1‑150 (MQ=255)
                                                                                                                                              ccTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTc        <  1:1663812/150‑1 (MQ=255)
                                                                                                                                               cTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCg       <  1:414832/150‑1 (MQ=255)
                                                                                                                                               cTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCg       <  1:1155399/150‑1 (MQ=255)
                                                                                                                                                 gTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGcc     <  1:619604/150‑1 (MQ=255)
                                                                                                                                                   tGATACAGGCCGGGAAAGGGATCAGGCAACATCCTGTACGCTGTGACCTTCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTg   <  1:33243/150‑1 (MQ=255)
                                                                                                                                                   tGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGCCGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTg   >  1:241716/1‑150 (MQ=255)
                                                                                                                                                    gATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTgc  >  1:1317679/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TCGAACCCAACCCTAAACCAATGCATAGGGGAACGGCGGCGTGCGTAATAACGGTTGGCATGATGGTCGCTTCGGCAAAATGTCGATGCTATCAGCATGGATGAACGGGGCGTAGAGGGCAAAAGTCTGAAAAGAGAACCGGCCTGTTGATACAGGCCGGGAAAGGGATCAGGCAACAACCTGTACGCTGTGACCTGCAAAACTCACTGTCTGACCGGCGACGATTTTGCAGCGTTTGCGCGTTTCAACCGCACCGTCGACTTTCACCTGGCCTTCGGCAATCGCGATTTTCGCCTGC  >  NC_000913/556493‑556790

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: