Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,774,215 A→G 5.5% intergenic (+21/‑209) mtlA → / → mtlD mannitol‑specific PTS enzyme: IIA, IIB and IIC components/mannitol‑1‑phosphate dehydrogenase, NAD‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,774,2150AG 5.5% 781.1 / 24.4 245intergenic (+21/‑209)mtlA/mtlDmannitol‑specific PTS enzyme: IIA, IIB and IIC components/mannitol‑1‑phosphate dehydrogenase, NAD‑dependent
Reads supporting (aligned to +/- strand):  ref base A (125/96);  new base G (8/5);  total (133/112)
Fisher's exact test for biased strand distribution p-value = 7.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.17e-01

Only 100 of 253 total aligned reads displayed.

GTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTACGCCGCATCCGGCGCTGCCCCTCTCCTCACGGAGAGGGTTTGG  >  NC_000913/3774067‑3774364
                                                                                                                                                    |                                                                                                                                                     
gTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACAt                                                                                                                                                      >  2:3182361/1‑150 (MQ=255)
 tAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATg                                                                                                                                                     <  2:3968873/150‑1 (MQ=255)
 tAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGACACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATg                                                                                                                                                     >  1:13166/1‑150 (MQ=255)
 tAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGACACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATg                                                                                                                                                     >  1:2173169/1‑150 (MQ=255)
  aacaacGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATgg                                                                                                                                                    <  1:764241/150‑1 (MQ=255)
  aacaacGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATgg                                                                                                                                                    <  1:2433078/150‑1 (MQ=255)
  aacaacGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATgg                                                                                                                                                    >  2:4261546/1‑150 (MQ=255)
    caacGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGgag                                                                                                                                                  >  2:2230824/1‑150 (MQ=255)
          gCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTg                                                                                                                                            >  1:1525717/1‑150 (MQ=255)
          gCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTg                                                                                                                                            >  1:1565772/1‑150 (MQ=255)
           cacaTTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCTTCTCCATGTGGAGAGGGTgg                                                                                                                                           <  1:845503/150‑1 (MQ=255)
           cacaTTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCTTCTCCATGTGGAGAGGGTgg                                                                                                                                           <  1:2678677/150‑1 (MQ=255)
           cacaTTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCTTCTCCATGTGGAGAGGGTgg                                                                                                                                           <  1:845474/150‑1 (MQ=255)
           cacaTTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTgg                                                                                                                                           <  1:159483/150‑1 (MQ=255)
            acaGTCAGGTTATCACCAGCCTGACCACTGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACCTGGAGAAGGTggg                                                                                                                                          <  2:2973108/150‑1 (MQ=255)
              aTTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGt                                                                                                                                        >  2:3142918/1‑150 (MQ=255)
               ttCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGtt                                                                                                                                       >  1:3676484/1‑150 (MQ=255)
               ttCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGtt                                                                                                                                       >  1:1393985/1‑150 (MQ=255)
                tCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACCCCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTCAGTAATCCAATCCCACCCTCTCCACAGGGAGAAGGGGGGGTTa                                                                                                                                      >  1:2270360/1‑150 (MQ=255)
                  aGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAAt                                                                                                                                    >  1:526964/1‑150 (MQ=255)
                   ggTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAAtt                                                                                                                                   <  2:2052075/150‑1 (MQ=255)
                    gTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTg                                                                                                                                  >  2:3617210/1‑150 (MQ=255)
                    gTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGGGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTg                                                                                                                                  >  1:1293135/1‑150 (MQ=255)
                     ttATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGc                                                                                                                                 <  1:1750222/150‑1 (MQ=255)
                           ccAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGATGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATg                                                                                                                           >  2:551211/1‑150 (MQ=255)
                           ccAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACCTGGAGAAGGTGGGGTTAATTGCCTGATg                                                                                                                           <  2:735289/150‑1 (MQ=255)
                           ccAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATg                                                                                                                           >  2:620752/1‑150 (MQ=255)
                           ccAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATg                                                                                                                           <  1:2360345/150‑1 (MQ=255)
                           ccAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATg                                                                                                                           >  2:2551077/1‑150 (MQ=255)
                              gCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATgcgc                                                                                                                        >  2:1918406/1‑150 (MQ=255)
                              gCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATgcgc                                                                                                                        >  2:2628310/1‑150 (MQ=255)
                              gCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATgcgc                                                                                                                        <  1:1185735/150‑1 (MQ=255)
                                     cAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCtt                                                                                                                 >  1:4098024/1‑150 (MQ=255)
                                          cACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAg                                                                                                            >  1:2856299/1‑150 (MQ=255)
                                          cACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAg                                                                                                            >  2:4065373/1‑150 (MQ=255)
                                          cACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAg                                                                                                            >  1:188865/1‑150 (MQ=255)
                                            cTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGc                                                                                                          <  1:1675405/150‑1 (MQ=255)
                                            cTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGc                                                                                                          <  1:1668514/150‑1 (MQ=255)
                                              ggATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCt                                                                                                        >  1:3871802/1‑150 (MQ=255)
                                                 tgatgaGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACa                                                                                                     >  1:1157311/1‑150 (MQ=255)
                                                 tgatgaGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTACTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACa                                                                                                     >  2:1512020/1‑150 (MQ=255)
                                                       gTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGc                                                                                               <  2:3975296/150‑1 (MQ=255)
                                                          cGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCCTc                                                                                            >  1:3078056/1‑150 (MQ=255)
                                                          cGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATc                                                                                            <  1:2560211/150‑1 (MQ=255)
                                                          cGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATc                                                                                            >  2:3755014/1‑150 (MQ=255)
                                                           gTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGAGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATca                                                                                           >  2:761632/1‑150 (MQ=255)
                                                               tCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCATATCAGGCCTACAGGATGCATCACAAt                                                                                       >  2:689037/1‑150 (MQ=255)
                                                                cGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAAtt                                                                                      <  1:3067924/150‑1 (MQ=255)
                                                                 gAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAAttt                                                                                     <  2:4095748/150‑1 (MQ=255)
                                                                   gCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATttgt                                                                                   <  1:2878335/150‑1 (MQ=255)
                                                                    cGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTACTCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATttgtt                                                                                  <  2:2803770/150‑1 (MQ=255)
                                                                    cGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATttgtt                                                                                  <  2:2940190/150‑1 (MQ=255)
                                                                       cTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGaa                                                                               <  1:2001300/150‑1 (MQ=255)
                                                                       cTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGaa                                                                               <  2:1877860/150‑1 (MQ=255)
                                                                        tGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAAt                                                                              >  1:3991928/1‑150 (MQ=255)
                                                                          gCACACACCACCAGCGTGGATGTAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAAttt                                                                            <  1:1783316/150‑1 (MQ=255)
                                                                          gCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAAttt                                                                            >  1:3287301/1‑150 (MQ=255)
                                                                          gCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAAttt                                                                            >  1:2459801/1‑150 (MQ=255)
                                                                          gCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAActt                                                                            >  1:452844/1‑150 (MQ=255)
                                                                           cacacaCCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGc                                                                                                          >  1:2583994/1‑119 (MQ=255)
                                                                                accaccAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTACTCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACACTTTGTTGTATTTGCACGt                                                                      >  2:875097/1‑150 (MQ=255)
                                                                                accaccAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGt                                                                      >  1:2910812/1‑150 (MQ=255)
                                                                                 ccaccaGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGtt                                                                     >  2:1030397/1‑150 (MQ=255)
                                                                                        cGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAg                                                              <  1:4091562/150‑1 (MQ=255)
                                                                                           ggATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACCTGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGcc                                                           <  2:2575324/150‑1 (MQ=255)
                                                                                            gATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCg                                                          <  2:3793172/150‑1 (MQ=255)
                                                                                            gATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCg                                                          <  2:1162933/150‑1 (MQ=255)
                                                                                             aTGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCgg                                                         >  2:3501629/1‑150 (MQ=255)
                                                                                                  gTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATacg                                                    >  2:3314150/1‑148 (MQ=255)
                                                                                                   tGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAgg                                                   <  2:411712/150‑1 (MQ=255)
                                                                                                     cTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGgcg                                                 >  2:1449367/1‑150 (MQ=255)
                                                                                                     cTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGgcg                                                 >  1:20691/1‑150 (MQ=255)
                                                                                                      tGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACCTGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGgcgc                                                <  2:2843338/150‑1 (MQ=255)
                                                                                                       ggAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCt                                               >  1:2407030/1‑150 (MQ=255)
                                                                                                          aCTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTAc                                            <  1:2011326/150‑1 (MQ=255)
                                                                                                             gctgGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTAcgcc                                         <  1:64887/150‑1 (MQ=255)
                                                                                                             gctgGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTAcgcc                                         <  1:2769965/150‑1 (MQ=255)
                                                                                                              ctgGCAGGTAGTAATTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGACTGAAGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTGGTAGGCCGGATAAGGCGCTTAcgccg                                        >  2:2366706/1‑150 (MQ=38)
                                                                                                               tgGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTAcgccgc                                       <  2:2040409/150‑1 (MQ=255)
                                                                                                                     gTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTACGCCGCATCCgg                                 <  2:1293135/150‑1 (MQ=255)
                                                                                                                       cGTAAGTAATCCAATCCCACCCTCACCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTACGCCGCATCCGgcg                               <  1:950624/150‑1 (MQ=255)
                                                                                                                         taagtaaTCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTACGCCGCATCCGGCGCt                             >  2:3862322/1‑150 (MQ=255)
                                                                                                                             taaTCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTACGCCGCATCCGGCGCTGccc                         <  2:2154733/150‑1 (MQ=255)
                                                                                                                             taaTCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTACGCCGCATCCGGCGCTGccc                         <  2:4072948/150‑1 (MQ=255)
                                                                                                                               aattaaccccACCTTCTCCATGTGGAGAGGGTGGGATTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTACGCCGCATCCGGCGCTGCCCct                       >  2:2189617/8‑150 (MQ=25)
                                                                                                                                tccaatccCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTCGGCCGGATAAGGCGCTTACGCCGCATCCGGCGCTGCCCctc                      >  1:1842248/1‑150 (MQ=255)
                                                                                                                                tccaatccCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTAcgc                                          <  2:1784908/130‑1 (MQ=255)
                                                                                                                                tccaatccCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTACGCCGCATCCGGCGCTGCCCctc                      >  1:3748118/1‑150 (MQ=255)
                                                                                                                                tccaatccCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTACGCCGCATCCGGCGCTGCCCctc                      >  1:3646315/1‑150 (MQ=255)
                                                                                                                                        cACCTTCTCCATGTGGAGAGGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTGGAATTTGCACGTGCTTGTAGGCCGGATAAGGCGCTTACGCCGCATCCGGCGCTGCCCCTCTCCTCACg              >  1:3138167/1‑150 (MQ=25)
                                                                                                                                          cccTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTACGCCGCATCCGGCGCTGCCCCTCTCCTCACGga            <  2:1621101/150‑1 (MQ=255)
                                                                                                                                            ctctCCACATGGATAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGAATCACAATTTGTTGAATTTGCACGTTCTTGTTGGCCGGATAAGGCGCTTACGCCGCATCCGGCGCTGCCCCTCTCCTCACGgaga          <  1:1015890/150‑1 (MQ=255)
                                                                                                                                            ctctCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGTATAAGGCGCTTACGCCGCATCCGGCGCTGCCCCTCTCCTCACGgaga          <  1:3781689/150‑1 (MQ=255)
                                                                                                                                             tctcCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTACGCCGCATCCGGCGCTGCCCCTCTCCTCACGGagag         >  1:1948036/1‑150 (MQ=255)
                                                                                                                                             tctcCACATGGAGAAGGTGGGGTTAATTGCCTGACGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTACGCCGCATCCGGCGCTGCCCCTCTCCTCACGGagag         <  2:2590832/150‑1 (MQ=255)
                                                                                                                                                ccACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTACGCCGCATCCGGCGCTGCCCCTCTCCTCACGGAGAGGGt      >  1:4220946/1‑150 (MQ=255)
                                                                                                                                                ccACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTACGCCGCATCCGGCGCTGCCCCTCTCCGTGAGGAGAgggg      <  2:2410963/150‑2 (MQ=255)
                                                                                                                                                 cacaTGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTACGCCGCATCCGGCGCTGCCCCTCTCCTCACGGAGAGGGtt     <  2:3287301/150‑1 (MQ=255)
                                                                                                                                                  acaTGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTACGCCGCATCCGGCGCTGCCCCTCTCCTCACGGAGAGGGttt    >  1:1049010/1‑150 (MQ=255)
                                                                                                                                                    aTGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTACGCCGCATCCGGCGCTGCCCCTCTCCTCACGGAGAGGGTTTgg  >  1:3513282/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTAATCCAATCCCACCCTCTCCACATGGAGAAGGTGGGGTTAATTGCCTGATGCGCTACGCTTATCAGGCCTACAGGATGCATCACAATTTGTTGAATTTGCACGTTCTTGTAGGCCGGATAAGGCGCTTACGCCGCATCCGGCGCTGCCCCTCTCCTCACGGAGAGGGTTTGG  >  NC_000913/3774067‑3774364

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: