Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,959,457 T→G 12.3% intergenic (+12/+75) ilvC → / ← ppiC ketol‑acid reductoisomerase, NAD(P)‑binding/peptidyl‑prolyl cis‑trans isomerase C (rotamase C)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,959,4570TG12.3% 577.4 / 19.9 217intergenic (+12/+75)ilvC/ppiCketol‑acid reductoisomerase, NAD(P)‑binding/peptidyl‑prolyl cis‑trans isomerase C (rotamase C)
Reads supporting (aligned to +/- strand):  ref base T (49/136);  new base G (29/2);  total (79/138)
Fisher's exact test for biased strand distribution p-value = 6.90e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 218 total aligned reads displayed.

GCTATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCG  >  NC_000913/3959308‑3959605
                                                                                                                                                     |                                                                                                                                                     
gCTATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGAt                                                                                                                                                       <  1:4134801/150‑1 (MQ=255)
 cTATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATg                                                                                                                                                      <  2:2710142/150‑1 (MQ=255)
  tATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGc                                                                                                                                                    <  2:2235871/150‑1 (MQ=255)
  tATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGc                                                                                                                                                    <  1:2364366/150‑1 (MQ=255)
  tATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGc                                                                                                                                                    >  1:2820554/1‑150 (MQ=255)
   aTTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGcc                                                                                                                                                   >  2:382353/1‑150 (MQ=255)
    ttCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGccc                                                                                                                                                  <  1:3794666/150‑1 (MQ=255)
             gcgcGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGa                                                                                                                                         <  2:1879385/150‑1 (MQ=255)
              cgcgGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGAc                                                                                                                                        <  2:1654910/150‑1 (MQ=255)
                cgGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTGAGTGCGCGCTGATGCCCTCACCCCGAccc                                                                                                                                      >  1:2108854/1‑150 (MQ=255)
                cgGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCACACCCCGAccc                                                                                                                                      >  1:2443846/1‑150 (MQ=255)
                 ggTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCct                                                                                                                                     >  1:1404286/1‑150 (MQ=255)
                  gTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGAGGGGCTCAcccccgcccac                                                                                                                                    >  2:59845/1‑143 (MQ=255)
                   tAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCGCACCCCCAcccaca                                                                                                                                   >  1:2188993/1‑147 (MQ=255)
                    aGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCtctc                                                                                                                                  <  1:958655/150‑1 (MQ=255)
                     gATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTcc                                                                                                                                 <  2:1205140/150‑1 (MQ=255)
                        aaCGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCcac                                                                                                                              <  1:2142737/150‑1 (MQ=255)
                         aCGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCcaca                                                                                                                             <  2:3800518/150‑1 (MQ=255)
                          cGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACag                                                                                                                            <  1:977366/150‑1 (MQ=255)
                           gggCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGTGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGTTATGAAAGGTATTGCTGTTGCGGGTTAAGTGGGCGCTGATGGCCTCACCCCGTGCCTCTACCACagg                                                                                                                           >  2:3330811/1‑150 (MQ=255)
                           gggCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTccctgtgg                                                                                                                           <  1:827564/150‑6 (MQ=255)
                               aaCTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGGCCGCACCCCCACCCACTCCCACaggggga                                                                                                                       >  2:2900339/1‑150 (MQ=255)
                               aaCTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACagggaga                                                                                                                       <  1:106506/150‑1 (MQ=255)
                                 cTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCTGtgggagagg                                                                                                                     <  1:3568263/150‑1 (MQ=255)
                                  tGCGTGATGTGAACGAAGCGATTCGCGGCCATGCGTTTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTATGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACagggagaggg                                                                                                                    <  1:2852571/150‑1 (MQ=255)
                                   gCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACagggagaggga                                                                                                                   <  1:351252/150‑1 (MQ=255)
                                     gTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAgggagagggaga                                                                                                                 <  2:1440685/150‑1 (MQ=255)
                                     gTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCACAACCCGCCCCTCTCCCACAgggagagggaga                                                                                                                 >  2:2976073/1‑150 (MQ=255)
                                       gATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCTGtgggagagggtcggg                                                                                                               <  2:322787/150‑6 (MQ=255)
                                        ctgtgAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGaaaa                                                                                                              <  1:1319314/149‑1 (MQ=255)
                                        aTGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGaaaa                                                                                                              <  1:4109824/150‑1 (MQ=255)
                                        aTGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGaaaa                                                                                                              <  1:3445946/150‑1 (MQ=255)
                                          gtgAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGATAGGGAGAAAaca                                                                                                            <  1:777406/150‑1 (MQ=255)
                                          gtgAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGAGGCCCGCACCCCCACCCCCAACCACACGGGGGGGGGGGAAaca                                                                                                            >  1:1456639/1‑150 (MQ=255)
                                           tgAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCACTCCCACAGGGAGAGGGAGAAAacac                                                                                                           >  1:2830254/1‑150 (MQ=255)
                                             aaCGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTc                                                                                                         <  2:3107776/150‑1 (MQ=255)
                                             aaCGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGAGGCCCGCACCCCCCCCCGCGCCCCCCCGGCGCGGGCGAAAacacgc                                                                                                         >  2:3035947/1‑148 (MQ=255)
                                               cGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCaa                                                                                                       <  1:186117/150‑1 (MQ=255)
                                                gAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAg                                                                                                      <  1:3131332/150‑1 (MQ=255)
                                                gAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCCACCCACACCCACAGGGAGAGGGAGTAAAAACACAAg                                                                                                      >  1:2989321/1‑150 (MQ=39)
                                                gAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGAGGGCCACACCCCCACCCACACCCACACGGAGAGGGAGGAAAAACTaaat                                                                                                      >  1:3254865/1‑149 (MQ=255)
                                                 ccgCGATGCGCAGCCATGCGAGTGAGCAGGTAGGTAAGAACCTGCGCGGCTATCTGACAGATATGACACGTATTGCTGTTGCGGGTTAAGTGCGCGCGGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAgg                                                                                                     <  2:831455/148‑1 (MQ=38)
                                                  agagATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGc                                                                                                    <  2:3737658/147‑1 (MQ=255)
                                                   gCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCACACCCCCACCCTAACCCCCACCGAGATGGCGAAAAAACACAAGGcc                                                                                                   >  2:449925/1‑150 (MQ=255)
                                                         cGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTcc                                                                                             <  1:4034832/150‑1 (MQ=255)
                                                          gCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCt                                                                                            <  2:3269664/150‑1 (MQ=255)
                                                          gCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCCACCCGCACCCACACGGAGAGGGAGAAAACACACAATGCCGTCTccg                                                                                            >  1:3942068/1‑149 (MQ=38)
                                                           cAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCGCACCCCGACCCGCTCCCACAGGGCGCGGGAGAAAACACACAAGGCCTTCTCCTg                                                                                           >  2:2603997/1‑150 (MQ=255)
                                                             gCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGga                                                                                         <  2:414383/150‑1 (MQ=255)
                                                             gCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGga                                                                                         <  1:2014949/150‑1 (MQ=255)
                                                             gCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGga                                                                                         <  2:2793555/150‑1 (MQ=255)
                                                                aTGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGaa                                                                                      <  1:1136879/150‑1 (MQ=255)
                                                                aTGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGaa                                                                                      <  1:2690549/150‑1 (MQ=255)
                                                                  gCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAgg                                                                                    <  1:2636121/150‑1 (MQ=255)
                                                                  gCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCACTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCACCTGGAGAAgg                                                                                    >  1:1711905/1‑150 (MQ=255)
                                                                   cGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGc                                                                                   <  2:2141224/150‑1 (MQ=255)
                                                                    gATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCAGGAGAAGGcc                                                                                  <  2:206472/150‑1 (MQ=255)
                                                                    gATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGAGGCCCGCACCCCCCCCCACACCCACCCGGAGAGGGCGGAAAAAAACAAGGCCCTCGACGGGAGAAgggc                                                                                  >  1:40265/1‑148 (MQ=255)
                                                                     aTTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCt                                                                                 <  2:1309591/150‑1 (MQ=255)
                                                                     aTTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCGCACCCACAGGGAGAGGGAGAAAACACACAAGGCCATCTCCGGGAGCAGGccg                                                                                 >  1:2767794/1‑149 (MQ=38)
                                                                      ttGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCAGGAGAAGGCCtt                                                                                <  2:490661/150‑1 (MQ=255)
                                                                        gAGCAGGTAGGTAAGAAACTGCGCGGATATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGTTGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCAGGAGAAGGCCTTga                                                                              <  1:1377605/150‑2 (MQ=255)
                                                                           cAGGTAGGTAAGATACTGCGCGGCTATATGACAGATATGAATCGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCGGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCAGGAGAAGGCCTTgagtg                                                                           <  2:1263100/150‑5 (MQ=38)
                                                                            aggtaggtAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCGCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTAtt                                                                          <  1:3224725/150‑1 (MQ=255)
                                                                              gtaggtaAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGAGGCCCGCACCCCGCCCCACGCCCACAGGGCGTGGGGGACAACACACAAGGCCATCTCCGGGCGAGGGCCGTgggtttag                                                                        >  1:1631897/1‑143 (MQ=255)
                                                                                   taAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTAATAGTAGCg                                                                   >  2:2750980/1‑150 (MQ=255)
                                                                                    aaGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCgg                                                                  <  1:4147874/150‑1 (MQ=255)
                                                                                    aaGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGAGGCCCCAAACCCCCACCCAAACCCCCCGGAGGGGGAGAAAACAAAAAACGCCCTAGACAGGAGAAGGCCGTGGCAATAGTTTCgg                                                                  >  2:2870632/1‑150 (MQ=255)
                                                                                     aGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCGCACCCCCACCCACACCCACAGGGAGTGGGCGAAAAAACACAAGGCCAACTCCAGGAGAAGGCCCTGCTAATAGTTGCGGt                                                                 >  2:3361235/1‑150 (MQ=255)
                                                                                      gAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTa                                                                <  1:3716915/150‑1 (MQ=255)
                                                                                       aaaCTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCACACCCCCACCCGCTCCCACACGGGGAGGGAGAAAAAACACAAAGCCATCACCAGGAGAAAGCCGTGCGATTAGTTTCGGttc                                                               >  2:67035/1‑148 (MQ=255)
                                                                                       aaaCTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGAGGCCCTCACCCCGACCCGCACCCCCAGGGAGGGGGAGGAAAAAATAAAGGCCTTCAACGGGTGAATGCCGTGGGATTATTTGCGGTAc                                                               >  1:491141/1‑150 (MQ=255)
                                                                                        aaCTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACa                                                              <  1:59845/150‑1 (MQ=255)
                                                                                        aaCTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACa                                                              <  1:206829/150‑1 (MQ=255)
                                                                                          cTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGc                                                            <  1:245778/150‑1 (MQ=255)
                                                                                           tGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCa                                                           <  2:4121575/150‑1 (MQ=255)
                                                                                              gcgGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGGCCGCACCCCCACCCACGACCACAGGGAGGGGGGGAAAAAACAAAAAGCCGTCTACAGGAGAAGGGCGGTCGATTAGTTGCGGGTCAGCAcca                                                        >  1:3335039/1‑149 (MQ=255)
                                                                                              gcgGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCGGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCt                                                        >  2:4250424/1‑150 (MQ=255)
                                                                                                ggCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGAGGCCCTCACCCCGACCCACAACCACACGGAGAGGGAGAAAACACACAAGGCCGTCACCTGGAGGAGGCCGTGGGCGTAGTTGGGGGACAGCACCATa                                                      >  1:2602866/1‑150 (MQ=255)
                                                                                                  cTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACCCTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAat                                                    <  2:294250/150‑1 (MQ=255)
                                                                                                  cTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAATGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAat                                                    <  2:2040744/150‑1 (MQ=255)
                                                                                                  cTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAat                                                    <  2:3963135/150‑1 (MQ=255)
                                                                                                    atatGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGAGGCCCGCACCCCCACCCACTCCCACAGGGAGAGGGGGTAAACACACAAGGCCATAAACTGGGGTAGGCCGGGCGAATTGTTTGGGTAAAGCAccgaaaaga                                                  >  1:1788868/1‑143 (MQ=255)
                                                                                                       tGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGAtgt                                               <  1:3560881/150‑1 (MQ=255)
                                                                                                         aCAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGa                                             <  2:1498556/150‑1 (MQ=255)
                                                                                                           aGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTAACCCCGACCCTCTCCCACAGGGCGAGGGAGAAAACACTCAAGGCCATCACCAGGAGAAGGCCGGTCTAATAGTTGACGTACAGCACCATAAAGATGTGaaa                                           >  2:4015301/1‑148 (MQ=255)
                                                                                                           aGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCGAACCCCCACCCAATACCACAGGGAGTGGGGGAAAACAATAAAATCCGTCGGCTGGAGGAGGGCGTGCTAATATTTGGGGTTCAACAACATAATTATGTGata                                           >  2:1581223/1‑150 (MQ=255)
                                                                                                           aGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCACACCCCGAACCACAACCACCGGGAGAGGGTGAAAAAACTCAATGCCATATACGTGAGTAGGCCGTTCTATTAGTTTCGGTACAGCAACATAAAGATGTGata                                           >  2:178932/1‑150 (MQ=255)
                                                                                                                  aaaCGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCAACCCGACCCACAACCACACGGAGAGGGTGAAAAAACACAAGGCCTTCTCCAGGAGAAAGCCTTGCTAATAGTTTCGGGTCACCACCGTAATGATTTGATATACCAAc                                    >  1:3063827/1‑150 (MQ=255)
                                                                                                                  aaaCGTATTGCTGTTGCGGGTTAAGTGCGCGCTGAGGCCCTCACCCCCACCCACTCCCACACGGAGTGGGAGAAAACACTCAAGGCCTTCTCCGGGCGAAGGCCGTGCGATTAGTTGCCGTGCAGCACCATAATGAGGTGATAACCgccc                                    >  2:2351662/1‑147 (MQ=255)
                                                                                                                  aaaCGTATTGCTGTTGCGGGTGAAGTGCGCGCTGATGCCCTCACCCCCACCCACACCCACACGGAGACGGCTAAAAAACTCAAAGCCCTCTACTGGGCAAGGCCTTGCTATTAGTTGCGGGAAAGCCCCATAAGGAAGGGATATACGAAc                                    >  2:179578/1‑150 (MQ=255)
                                                                                                                     cGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTgg                                 <  1:1900687/150‑1 (MQ=255)
                                                                                                                       tATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGgt                               <  1:2884173/150‑1 (MQ=255)
                                                                                                                           gCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGc                           <  1:2281149/150‑1 (MQ=255)
                                                                                                                               ttGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCCCAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTCGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCg                       <  2:2265235/150‑1 (MQ=255)
                                                                                                                                tGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCgg                      <  2:1060450/150‑1 (MQ=255)
                                                                                                                                  cGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGc                    <  2:3479707/150‑1 (MQ=255)
                                                                                                                                            gcgcgcTGAGGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTc          <  1:2933358/150‑1 (MQ=255)
                                                                                                                                             cgcgcTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTGCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTcc         <  2:2435448/150‑1 (MQ=255)
                                                                                                                                                  tGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTAc    <  2:1677645/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCTATTCCGGAAGGCGCGGTAGATAACGGGCAACTGCGTGATGTGAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCG  >  NC_000913/3959308‑3959605

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: