BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	781,254	A→T	5.3%	intergenic (+32/‑115)	lysY → / → lysZ	tRNA‑Lys/tRNA‑Lys

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	781,254	0	A	T	5.3%	681.2 / 15.8	219	intergenic (+32/‑115)	lysY/lysZ	tRNA‑Lys/tRNA‑Lys
Reads supporting (aligned to +/- strand): ref base A (110/94); new base T (4/9); total (115/104)
Fisher's exact test for biased strand distribution p-value = 1.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 224 total aligned reads displayed.

GGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTT  >  NC_000913/781106‑781403

ggtgggggTCGGGGGTTCGATCCCGTCAACACCCACCCCTCGGGTCGTGAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGaa                                                                                                                                                      <  1:3916921/147‑1 (MQ=14)

   gggggTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCCGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCAGCACGACCCACCAGTTTTAACATCAAACGCAGATGTTAAGCGGGACGGa                                                                                                                                                   >  1:3739548/1‑150 (MQ=14)

        tCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACg                                                                                                                                              <  1:3699279/150‑1 (MQ=255)

           gcggTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCCGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGTTAACGTTg                                                                                                                                           >  1:1887567/1‑150 (MQ=255)

           gcggTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCAGCACGACCCACCAGTTTTAACATCAAAAACAGATGTTAAGCGTGAAGGATAAAGTTg                                                                                                                                           >  1:4016686/1‑150 (MQ=255)

            cggTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTgc                                                                                                                                          <  2:2350029/150‑1 (MQ=255)

            cggTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTgc                                                                                                                                          <  2:2850414/150‑1 (MQ=255)

             ggTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTgcg                                                                                                                                         >  2:3048241/1‑150 (MQ=255)

              gTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTgcgc                                                                                                                                        <  2:1254181/150‑1 (MQ=255)

               ttCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGcc                                                                                                                                       >  1:934820/1‑150 (MQ=255)

                tCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCCAACGCAGATGTTAACCGTGACCGCAAACGATGCGCCa                                                                                                                                      >  1:3506735/1‑150 (MQ=14)

                tCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAACCGTGAAGGATAACGTTGCGCCa                                                                                                                                      >  1:618566/1‑150 (MQ=255)

                  gATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGAAAACGTTGCGCCAGc                                                                                                                                    >  1:2787757/1‑150 (MQ=255)

                   aTCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAAAATCAAACTCAGATGTTAATCGTGAAGGATAACGTTGCGCCAGCa                                                                                                                                   >  1:385191/1‑150 (MQ=255)

                      ccGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACg                                                                                                                                <  2:1722002/150‑1 (MQ=255)

                      ccGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACg                                                                                                                                <  2:3398781/150‑1 (MQ=255)

                            tcaCCCACCACTCGGGTCGTTAGCTCAGGTGGTAGAGCAGTTGACTGTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGGTTTAACATCAAACTCAGAAGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCga                                                                                                                          >  1:4188504/1‑149 (MQ=255)

                             cacccaccACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTa                                                                                                                         <  2:3069763/150‑1 (MQ=255)

                              acccaccACTCGGGTCATTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGAAGATAAGCGTGAAGGAAACCGTTCCGACAGCAACGGCCCGGAg                                                                                                                        >  2:3750375/1‑150 (MQ=14)

                               cccaccACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAAACAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCTAACTCAGATGGTAAGCGTGAAGGATAACGTGGCGCCAGCACCGGCCCGTAgg                                                                                                                       >  2:1163363/1‑150 (MQ=14)

                               cccaccAC‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACACAGATGTTAAGCGTGAAGGATAACGTTGCGACAGCAACGGCCCGTAggg                                                                                                                      >  2:4164280/1‑150 (MQ=2)

                                ccaccaCTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTAAACATCAAAAAAAGTAGTTAAGCGTGAAGGAAAAAGATCCGACAGCACCGGCCCGttgcg                                                                                                                      >  2:3221391/1‑146 (MQ=11)

                                ccaccaC‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCa                                                                                                                                      <  1:887806/133‑1 (MQ=255)

                                  accacTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGgcg                                                                                                                    <  2:1053881/150‑1 (MQ=255)

                                    cacTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGgcgag                                                                                                                  <  2:2467356/150‑1 (MQ=255)

                                        cGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGagcgaa                                                                                                              <  2:836661/150‑1 (MQ=255)

                                         gggTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGagcgaag                                                                                                             <  2:3882736/150‑1 (MQ=255)

                                         gggTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGagcgaag                                                                                                             <  2:2985349/150‑1 (MQ=255)

                                         gggTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTAAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGCCCCGTAGGGCGagcgaag                                                                                                             >  2:2385878/1‑150 (MQ=255)

                                              gTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGt                                                                                                        <  2:917084/150‑1 (MQ=255)

                                                  gCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATc                                                                                                    <  2:3590854/150‑1 (MQ=255)

                                                       gTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGGTGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCa                                                                                               >  2:818594/1‑150 (MQ=255)

                                                        ttGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCAc                                                                                              >  2:3377730/1‑150 (MQ=255)

                                                              gagCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACcca                                                                                        <  2:3144308/150‑1 (MQ=255)

                                                               agCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACccac                                                                                       <  2:2252618/150‑1 (MQ=255)

                                                                gCAGTTGACTTTTAATAAATTTGTCGCAGGTTCGAATCCTGCACGACACACCATTTTTATCATAAAACTCAGATGTTAATCGTGATGGTTAAGGTTGAGCCAGCAACGGCCCGTAGGGCTTTAGAAGAGAGTCATACTGCACGaaacaac                                                                                      >  1:2117713/1‑144 (MQ=14)

                                                                    ttGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATc                                                                                  <  2:1646752/150‑1 (MQ=255)

                                                                        gaggcactCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCATACTCAGATGTTAAGCGTGAAGTATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTaa                                                                              <  2:3929792/143‑1 (MQ=255)

                                                                         ttttAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAAGTCAGATGTTAAGCGTGTAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTaaa                                                                             <  2:2174799/150‑1 (MQ=255)

                                                                          tttAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAg                                                                            >  2:76948/1‑150 (MQ=255)

                                                                              aTCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCATAAAGATTg                                                                        >  1:3121419/1‑150 (MQ=255)

                                                                              aTCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATACTGCACGACCCACAAATCTTAAAGATTg                                                                        >  2:3984430/1‑150 (MQ=255)

                                                                               tCATTTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATAAAACTCTTATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTgg                                                                       <  1:2261094/150‑1 (MQ=255)

                                                                                 aaTTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGcc                                                                     >  1:1497717/1‑150 (MQ=255)

                                                                                  aTTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTTAAGATTggtgg                                                                    <  1:1346138/150‑4 (MQ=255)

                                                                                   ttGGTCGCAGGTTCGTATCCTGCACGACCCACCATTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGcccc                                                                   <  1:2952702/150‑1 (MQ=255)

                                                                                   ttGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGcccc                                                                   <  2:3082069/150‑1 (MQ=255)

                                                                                   ttGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGcccc                                                                   >  1:220675/1‑150 (MQ=255)

                                                                                     ggTCGCAGGTTCGATTCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATTTTAAGCGTTAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGa                                                                 <  1:1619694/150‑1 (MQ=255)

                                                                                     ggTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGa                                                                 <  2:3783629/150‑1 (MQ=255)

                                                                                     ggTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGa                                                                 <  1:2378174/150‑1 (MQ=255)

                                                                                       tCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGt                                                               >  2:2636937/1‑150 (MQ=255)

                                                                                       tCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGt                                                               >  2:418558/1‑150 (MQ=255)

                                                                                       tCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGt                                                               >  1:2680303/1‑150 (MQ=255)

                                                                                           aGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTaaaa                                                           >  1:337202/1‑150 (MQ=11)

                                                                                           aGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTaaaa                                                           <  1:76948/150‑1 (MQ=255)

                                                                                             gTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCa                                                                                                                                      >  2:1260756/1‑73 (MQ=255)

                                                                                             gTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCa                                                                                                                                      <  1:1260756/73‑1 (MQ=255)

                                                                                             gTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAAt                                                         >  1:980403/1‑150 (MQ=255)

                                                                                              ttCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGACGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCCATCTTAAGGTTTGGCCCCGAGTaaaaagc                                                        >  1:1857356/1‑148 (MQ=255)

                                                                                              ttCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATc                                                        >  1:3527154/1‑150 (MQ=255)

                                                                                               tCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCt                                                       <  2:3525038/150‑1 (MQ=255)

                                                                                                 gcaTCCGGCACGACCCACCATTTTTAACATCTAAGTCTGATGTTTAGCGTTTTGGTTTTGTTTGGTGGTGATACGGGTGGTATTGCGATCGAAGGGTGTAATCCTGCACGACCCACCATTCTTAAAGATTGGCCCCGAGTAAAAATCttt                                                     <  1:3439463/148‑1 (MQ=255)

                                                                                                 gAATCCTGCACGACCCACCAGTTTTAACATCTAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCttt                                                     <  2:2134328/150‑1 (MQ=255)

                                                                                                 gAATCCTGCACGACCCACCAGTTTTAACATCAAACACAGAGGGGAAGCGTGAATGAAAACGTTCCTCCAGCAACGGCCCGGAGGGCGCGCGCAGCGAGTCATCCTGCACCACCCACCCATCTTAAAGAGTGGCCCCGCGTAAAAATCtat                                                     >  2:2745521/1‑148 (MQ=17)

                                                                                                  aaTCCTGCACGACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTATAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTc                                                    >  2:603481/1‑150 (MQ=14)

                                                                                                   aTCCTGCACGACCCACCAGTTTTAACATCTAAGTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCa                                                   <  2:156835/150‑1 (MQ=255)

                                                                                                   aTCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCa                                                   <  2:1135108/150‑1 (MQ=255)

                                                                                                   aTCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCa                                                   <  1:1139051/150‑1 (MQ=255)

                                                                                                   aTCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCa                                                   <  1:3639202/150‑1 (MQ=255)

                                                                                                   aTCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCa                                                   <  2:1511262/150‑1 (MQ=255)

                                                                                                     ccTGCACGACCCACCAGTTTTCACATCATACTAAGTTGTTTTGCGTTTTTGATAACGTTGCGGCTGCAACGTCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAgg                                                 <  1:1035515/150‑1 (MQ=18)

                                                                                                     ccTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAgg                                                 >  2:533662/1‑150 (MQ=255)

                                                                                                     ccTGCACGACCCACCAGTTTTAACATAAAACTAAGATGTTAAGCGTGTTGTTTTTGGTTGAGTCTTCAACTGCCCGTAGGGCTAGCGAATCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAgg                                                 <  1:388804/150‑1 (MQ=18)

                                                                                                      cTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGt                                                >  1:519690/1‑150 (MQ=255)

                                                                                                           cGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAacac                                           <  1:1816585/150‑1 (MQ=255)

                                                                                                                caccaGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTa                                      <  2:4196952/150‑1 (MQ=255)

                                                                                                                   caGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATgg                                   <  1:2314279/150‑1 (MQ=255)

                                                                                                                      ttttAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTc                                >  1:2922683/1‑150 (MQ=255)

                                                                                                                      ttttAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAAACTTTCAGGTAACACCCGTATGGGTc                                >  1:472991/1‑150 (MQ=255)

                                                                                                                         tAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGtt                             >  1:1871949/1‑150 (MQ=255)

                                                                                                                                    tCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTgg                  >  2:1587459/1‑150 (MQ=255)

                                                                                                                                    tCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTgg                  <  2:2031833/150‑1 (MQ=255)

                                                                                                                                    tCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAAACTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTgg                  >  1:3319553/1‑150 (MQ=255)

                                                                                                                                      aGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTa                >  1:3260971/1‑150 (MQ=255)

                                                                                                                                      aGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTa                <  1:1353673/150‑1 (MQ=255)

                                                                                                                                      aGATGATAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTa                <  2:2270968/150‑1 (MQ=255)

                                                                                                                                        ttGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTaga              <  2:3918927/149‑1 (MQ=255)

                                                                                                                                        aTGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGGTTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTaga              <  2:880822/150‑1 (MQ=255)

                                                                                                                                         tGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGccc                                                                    >  2:3351687/1‑95 (MQ=255)

                                                                                                                                             aaGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTCACACCCGTATGGGTCGTTAGCTCAGGTGGTAGAGCAGt         >  2:4104668/1‑150 (MQ=255)

                                                                                                                                                cttGAAGCATAACGTTGCGCAATCAACGGCCCGTAGGGCGAGCGAAGCGATTCATACTGCACGACCAACCAATCATAAAGATTGGCCCCTAGTAAAAATCTTTCATGTAACAACCGTATGGGTCGTTAGCTCAGTTTGTAGAGCAGTTGa      <  1:1166246/148‑1 (MQ=37)

                                                                                                                                                cGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGa      <  1:3143990/150‑1 (MQ=255)

                                                                                                                                                 gTGGAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGAc     <  1:1068853/150‑1 (MQ=255)

                                                                                                                                                 gTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGAc     <  1:3209876/150‑1 (MQ=255)

                                                                                                                                                  tGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGTTTGGCCCCGTGTAATAATCTTTCAGGTAACTGCCGTCTGGGTGGTTAGCTGAGTTGGTAGAGGAGTTGagt    >  2:252384/1‑148 (MQ=25)

                                                                                                                                                  tGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCGCGAGTAAAATTCTTTCAGGTAACTCCCGTATTGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACt    >  2:702357/1‑150 (MQ=255)

                                                                                                                                                  tGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACt    >  1:2891557/1‑150 (MQ=255)

                                                                                                                                                   gAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACtt   >  1:2570886/1‑150 (MQ=255)

                                                                                                                                                    aaGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAATGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACtgt  >  1:2137311/1‑148 (MQ=255)

GGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTT  >  NC_000913/781106‑781403

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`