Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 950,313 C→T 5.6% intergenic (+55/+27) ycaK → / ← pflA putative NAD(P)H‑dependent oxidoreductase/pyruvate formate‑lyase 1‑activating enzyme; [formate‑C‑acetyltransferase 1]‑activating enzyme; PFL activase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913950,3130CT 5.6% 794.0 / 22.7 232intergenic (+55/+27)ycaK/pflAputative NAD(P)H‑dependent oxidoreductase/pyruvate formate‑lyase 1‑activating enzyme; [formate‑C‑acetyltransferase 1]‑activating enzyme; PFL activase
Reads supporting (aligned to +/- strand):  ref base C (125/94);  new base T (4/9);  total (129/103)
Fisher's exact test for biased strand distribution p-value = 8.49e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 234 total aligned reads displayed.

GTGTAATACAGTGGTATTCGATGGGGAAGAACTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCATTTGTGTTT  >  NC_000913/950165‑950462
                                                                                                                                                    |                                                                                                                                                     
gtgtAATACAGTGGTATTCGATGGGGAAGAACTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCa                                                                                                                                                      >  2:682805/1‑150 (MQ=255)
gtgtAATACAGTGGTATTCGATGGGGAAGAACTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCa                                                                                                                                                      >  2:3618008/1‑150 (MQ=255)
 tgtAATACAGTGGTATTCGATGGGGAAGAACTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCaa                                                                                                                                                     >  1:3541582/1‑150 (MQ=255)
  ttactACTGTGTTATTCGATGGGGAAGAACTTCACGCGAGCTTTTATCAGTCGTTATTATCTCTGGTACGGGATTTGTTAGATGCACTATATGTTGTTTAAAAAACGCTTTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAAt                                                                                                                                                    <  1:4004521/146‑1 (MQ=255)
   tAATACAGTGGTATTCGATGGGGAAGAACTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATa                                                                                                                                                   >  1:102654/1‑150 (MQ=255)
        ctgTGGTATTCGTTGGGTAATAACTTCACGCGAGATATTATCAGTCTTTATTATCTCTGGTACGGTATTTGTTAGTTGAACTATATGTTGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGTAATAAAAAAGCGGCAACTCAATAAAGtt                                                                                                                                              <  1:4011122/148‑1 (MQ=255)
           tGGTATTCGATGGGGAAGAACTTCACGCGAGCTATTATCAGTCGTTATTATATAACGTAATGGATATGGTATATGAACTATAAGATGTTTTAAAAACCCAGTATCAGAATGAAAACCCGAAAAAAAAAGAGGCAACACACTAAAgattcc                                                                                                                                           >  1:3914397/1‑145 (MQ=255)
           tGGTATTCGATGGGGAAGAACTTCACGCGAGATATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGcc                                                                                                                                           <  1:2382348/150‑1 (MQ=255)
            ggTATTGGTGGGGGAAGAACTTCACGCGCGCTATTATCAGTCGTTATTATCTCCGGTACGGGATATGGTAGATGCACTATAAGATTTGTTAAAAACGCTTTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCg                                                                                                                                          <  1:3687593/150‑1 (MQ=255)
            ggTATTCGATGGGGAAGAACTTCTCGCTAGCTTTTATCAGTCGTTTTTATCTCTGGTACGGTTTTTGGTAGATGCACTATATTATGTGTTAAAAACGCTTTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTTTATTGAGTTGCCg                                                                                                                                          <  1:2672859/150‑1 (MQ=255)
             gTATTCGATGGGGAAGAACTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTTTATTGAGTTGCCGc                                                                                                                                         <  2:3256681/150‑1 (MQ=255)
             gTATTCGATGGGGAAGAACTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTTTATTGAGTTGCCGc                                                                                                                                         <  2:627385/150‑1 (MQ=255)
              tATTCGATGGGGAAGAACTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATTTGTTAAAAAAGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTTCCGCt                                                                                                                                        >  1:748661/1‑150 (MQ=255)
              tATTCGATGGGGAAGAACTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCt                                                                                                                                        >  2:2724274/1‑150 (MQ=255)
                ttCGATGGGGAAGAACTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGTTGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAATTGCGGCAACTCAATAAAGTTTCCGCttt                                                                                                                                      >  1:674507/1‑150 (MQ=255)
                ttCGATGGGGAAGAACTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTTTATTGAGTTGCCGCttt                                                                                                                                      <  2:2219988/150‑1 (MQ=255)
                  cGATGGGGAAGAACTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTAc                                                                                                                                    >  2:888132/1‑150 (MQ=255)
                  cGATGGGGAAGAACTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCGGTAGCAGAATGAAGCGCGGAATAAAAAATCGGCAACTCAATAAAGTTGCCGCTTTAc                                                                                                                                    >  1:1440785/1‑150 (MQ=255)
                         gaagaaCTTCACGCGCGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCCCGATAACATGTGTTAAAAACGCGGTAGCAGAATGAAGCGCGGCATAAAAAAGCGGCAACGCAAAAAAGTTGCCGCGTTACGGGGaaa                                                                                                                             >  1:2630700/1‑150 (MQ=255)
                          aagaaCTTCACGCGAGCTATTATCAGTCGTGATTATCTCAGGTACGGGCTATGGTAGATGCACAATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAAt                                                                                                                            >  1:3592136/1‑150 (MQ=255)
                            gaaCTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTa                                                                                                                          >  2:3402762/1‑150 (MQ=255)
                            gaaCTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGCGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCAGTAGCAGAATGAAGAGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTa                                                                                                                          >  2:77065/1‑150 (MQ=255)
                               cTTCACTCTAGCTATTATCTGTCGTTATTATCTCAGGTACGGGATATGGTAGATGAACTATAAGATGTGTTATAAACGCTGTATCTTATTGAAGCGCGGAATAATAAAGAGGCTACTCAATAAAGTTGCCGCTTTACGGGTAAATTTGaa                                                                                                                       <  2:3598737/150‑1 (MQ=255)
                               cTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGaa                                                                                                                       <  2:2297361/150‑1 (MQ=255)
                                ttCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAAc                                                                                                                      >  2:4229991/1‑150 (MQ=255)
                                ttCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAAc                                                                                                                      >  2:3955385/1‑150 (MQ=255)
                                ttCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGAACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAAc                                                                                                                      >  1:3456230/1‑150 (MQ=255)
                                ttCACGCGAGCTATTATCAGTCGTTATTATCTAAGGTACCGGATATGGTAGATGCACGATAAGATTTTTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACACAAAAAAGTTTCCGCATTACGGGGAAAATAGAAc                                                                                                                      >  1:1038944/1‑150 (MQ=255)
                                ttCACGCGAGCTATTATCAGTCGTTATTATATCAGGTACGGGATATTGTAGATGCACAATAAGATGTTTTTAAAAAGCTGTAGCAGAATTAAGCGCGGAATAAAAAATCGGCAACTCAAAAAAGTTTCCGCTTTAAGGGGAAATTAGaaa                                                                                                                      >  1:374842/1‑149 (MQ=255)
                                   aCGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGGTATGGTAGGTGCACAATAATATGTGTTAAAAAAGCTGTAGCAGAAAGAAGCGCGGAATAAAAAATCGGCAACACAATAAAGTTGCCGCTTTAAGGGGAAAATATaaaaat                                                                                                                   >  1:841934/1‑146 (MQ=255)
                                   aCGCGAGCTATTATCAGTCGTGATTATCTCAGGGACGGGATATGGGAGATGCACAATAAGATTTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCCACACAATAAACTTTCCGCTTTAAGGGGAACTTAGAACaat                                                                                                                   >  1:1253654/1‑148 (MQ=255)
                                      cgAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTTGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTAcc                                                                                                                <  1:1429282/150‑1 (MQ=255)
                                          cTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTAt                                                                                                            <  1:3834207/150‑1 (MQ=255)
                                           tattatCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTAtt                                                                                                           >  2:3970739/1‑149 (MQ=255)
                                                tCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGt                                                                                                      >  2:92639/1‑150 (MQ=255)
                                                    tCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTg                                                                                                  <  2:3496893/150‑1 (MQ=255)
                                                       ttattaTCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTc                                                                                               >  1:3054610/1‑150 (MQ=255)
                                                       ttattaTCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTc                                                                                               >  1:552947/1‑150 (MQ=255)
                                                          ttatCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAg                                                                                            <  1:3208139/150‑1 (MQ=255)
                                                          ttatCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAg                                                                                            >  2:3861317/1‑150 (MQ=255)
                                                               tcAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGACATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGc                                                                                       >  2:4009612/1‑150 (MQ=255)
                                                               tcAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGc                                                                                       >  2:3016387/1‑150 (MQ=255)
                                                               tcAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGc                                                                                       >  1:2872010/1‑150 (MQ=255)
                                                               tcAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGc                                                                                       <  1:706698/150‑1 (MQ=255)
                                                               tcAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGc                                                                                       >  1:212195/1‑150 (MQ=255)
                                                               tcAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGc                                                                                       >  2:3295420/1‑150 (MQ=255)
                                                               tcAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGc                                                                                       >  1:2242450/1‑150 (MQ=255)
                                                                 aGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCt                                                                                     <  2:3541582/150‑1 (MQ=255)
                                                                    tACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAATCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTc                                                                                  >  1:2313247/1‑150 (MQ=255)
                                                                     aCGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCa                                                                                 >  2:2182540/1‑150 (MQ=255)
                                                                              ggTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCa                                                                        <  1:2845101/150‑1 (MQ=255)
                                                                               gTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCAt                                                                       >  1:3341128/1‑150 (MQ=255)
                                                                                      cACTATAAGATGTGTTAAAAACGCTGTAGAAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTctct                                                                <  2:726883/150‑1 (MQ=255)
                                                                                      cACTATAAGATGTGATAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCATTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTctct                                                                <  1:130481/150‑1 (MQ=255)
                                                                                             aGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCACCTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCg                                                         >  2:1519044/1‑150 (MQ=255)
                                                                                             aGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCg                                                         <  2:3914369/150‑1 (MQ=255)
                                                                                             aGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCg                                                         >  2:532687/1‑150 (MQ=255)
                                                                                              gATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCgg                                                        >  2:298840/1‑150 (MQ=255)
                                                                                               aTGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCggt                                                       >  1:1734115/1‑150 (MQ=255)
                                                                                               aTGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCggt                                                       >  1:975428/1‑150 (MQ=255)
                                                                                               aTGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCggt                                                       <  2:3773648/150‑1 (MQ=255)
                                                                                               aTGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCggt                                                       <  1:2470134/150‑1 (MQ=255)
                                                                                                 gtgtTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATTCCTTTCACGCGTTCCATGGTCTCTTTCTTCggtgg                                                     <  2:3458336/150‑1 (MQ=255)
                                                                                                  tgtTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTACTTCggtggt                                                    >  2:3213174/1‑150 (MQ=255)
                                                                                                   gtTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGtt                                                   >  1:1925680/1‑150 (MQ=255)
                                                                                                   gtTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACAGTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGtt                                                   <  1:4167430/150‑1 (MQ=255)
                                                                                                    ttAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGttt                                                  >  2:1181073/1‑150 (MQ=255)
                                                                                                    ttAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGttt                                                  >  1:2006738/1‑150 (MQ=255)
                                                                                                    ttAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGttt                                                  >  1:917384/1‑150 (MQ=255)
                                                                                                    ttAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGttt                                                  >  2:2344974/1‑150 (MQ=255)
                                                                                                      aataaCGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGATCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTaa                                                <  1:2532585/147‑1 (MQ=255)
                                                                                                             cTGTAGCAGAATGAAGCTCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTc                                         <  1:1674674/150‑1 (MQ=255)
                                                                                                                  gCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAACTTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGtt                                    >  2:1331277/1‑150 (MQ=255)
                                                                                                                  gCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGtt                                    >  1:3008504/1‑150 (MQ=255)
                                                                                                                   cAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGttt                                   >  2:2025719/1‑150 (MQ=255)
                                                                                                                     gAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAATTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGt                                 <  2:952872/150‑1 (MQ=255)
                                                                                                                          aaGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTActct                            >  2:2806432/1‑150 (MQ=255)
                                                                                                                            gcgcGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTc                          <  1:3869448/150‑1 (MQ=255)
                                                                                                                             cgcgGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCa                         <  2:3194310/150‑1 (MQ=255)
                                                                                                                              gcgGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCAc                        <  2:3052685/150‑1 (MQ=255)
                                                                                                                                   aTAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCAtt                   <  1:1198158/150‑1 (MQ=255)
                                                                                                                                   aTAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCAtt                   <  1:2236902/150‑1 (MQ=255)
                                                                                                                                    ccgtaaaGCGGCAACTTTATTGAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTg                  >  2:12451/5‑150 (MQ=255)
                                                                                                                                     aaaaaaGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACAGTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGc                 <  2:2768644/150‑1 (MQ=255)
                                                                                                                                     aaaaaaGCCGCAACACAATAAAGTTGCCGCATTACGGCGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGc                 <  2:4129277/150‑1 (MQ=255)
                                                                                                                                         aaGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCAcc             <  1:1569833/150‑1 (MQ=255)
                                                                                                                                          aGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCAccc            >  2:3202296/1‑150 (MQ=255)
                                                                                                                                           gCGGCAACTTTATTGAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCa           >  2:430576/1‑150 (MQ=255)
                                                                                                                                              gCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCAttt        >  1:117045/1‑150 (MQ=255)
                                                                                                                                              gCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCAGTGCCACCCAttt        <  2:2130649/150‑1 (MQ=255)
                                                                                                                                               cAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCATTtg       >  2:2427955/1‑150 (MQ=255)
                                                                                                                                                aaCTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCATgtgt      >  2:1535511/1‑150 (MQ=255)
                                                                                                                                                aaCTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCATTtgt      >  2:1653355/1‑150 (MQ=255)
                                                                                                                                                aaCTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCATTtgt      >  2:1964996/1‑150 (MQ=255)
                                                                                                                                                aaCTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCATTtgt      >  1:2771211/1‑150 (MQ=255)
                                                                                                                                                 aCTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCATTtgtg     >  1:2352387/1‑150 (MQ=255)
                                                                                                                                                  cTCAATAAAGTTGCCGCTTGACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCATTTgtgt    <  1:4043700/150‑1 (MQ=255)
                                                                                                                                                   tCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCATTTGTGtt   >  2:3420747/1‑150 (MQ=255)
                                                                                                                                                   tCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCATTTGTGtt   <  1:1217146/150‑1 (MQ=255)
                                                                                                                                                    cAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCATTTGTGttt  <  2:2242450/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GTGTAATACAGTGGTATTCGATGGGGAAGAACTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCATTTGTGTTT  >  NC_000913/950165‑950462

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: