Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 950,314 A→T 5.7% intergenic (+56/+26) ycaK → / ← pflA putative NAD(P)H‑dependent oxidoreductase/pyruvate formate‑lyase 1‑activating enzyme; [formate‑C‑acetyltransferase 1]‑activating enzyme; PFL activase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913950,3140AT 5.7% 816.5 / 27.0 230intergenic (+56/+26)ycaK/pflAputative NAD(P)H‑dependent oxidoreductase/pyruvate formate‑lyase 1‑activating enzyme; [formate‑C‑acetyltransferase 1]‑activating enzyme; PFL activase
Reads supporting (aligned to +/- strand):  ref base A (121/96);  new base T (4/9);  total (125/105)
Fisher's exact test for biased strand distribution p-value = 9.18e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.22e-01

Only 100 of 236 total aligned reads displayed.

GTGTAATACAGTGGTATTCGATGGGGAAGAACTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCATTTGTGTTTG  >  NC_000913/950165‑950463
                                                                                                                                                     |                                                                                                                                                     
gtgtAATACAGTGGTATTCGATGGGGAAGAACTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCa                                                                                                                                                       >  2:682805/1‑150 (MQ=255)
 tgtAATACAGTGGTATTCGATGGGGAAGAACTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCaa                                                                                                                                                      >  1:3541582/1‑150 (MQ=255)
  ttactACTGTGTTATTCGATGGGGAAGAACTTCACGCGAGCTTTTATCAGTCGTTATTATCTCTGGTACGGGATTTGTTAGATGCACTATATGTTGTTTAAAAAACGCTTTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAAt                                                                                                                                                     <  1:4004521/146‑1 (MQ=255)
   tAATACAGTGGTATTCGATGGGGAAGAACTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATa                                                                                                                                                    >  1:102654/1‑150 (MQ=255)
        ctgTGGTATTGGTTGGGTAAGAACTTCACGCTAGCTATTATCAGTCGTTATTATCTCAGGTACGGTATATGGTAGATGCACTATAAGTTGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGtt                                                                                                                                               <  1:2613056/148‑1 (MQ=255)
        ctgTGGTATTCGTTGGGTAATAACTTCACGCGAGATATTATCAGTCTTTATTATCTCTGGTACGGTATTTGTTAGTTGAACTATATGTTGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGTAATAAAAAAGCGGCAACTCAATAAAGtt                                                                                                                                               <  1:4011122/148‑1 (MQ=255)
           tGGTATTCGATGGGGAAGAACTTCACGCGAGCTATTATCAGTCGTTATTATATAACGTAATGGATATGGTATATGAACTATAAGATGTTTTAAAAACCCAGTATCAGAATGAAAACCCGAAAAAAAAAGAGGCAACACACTAAAgattcc                                                                                                                                            >  1:3914397/1‑145 (MQ=255)
            ggTATTGGTGGGGGAAGAACTTCACGCGCGCTATTATCAGTCGTTATTATCTCCGGTACGGGATATGGTAGATGCACTATAAGATTTGTTAAAAACGCTTTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCg                                                                                                                                           <  1:3687593/150‑1 (MQ=255)
            ggTATTCGATGGGGAAGAACTTCTCGCTAGCTTTTATCAGTCGTTTTTATCTCTGGTACGGTTTTTGGTAGATGCACTATATTATGTGTTAAAAACGCTTTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTTTATTGAGTTGCCg                                                                                                                                           <  1:2672859/150‑1 (MQ=255)
             gTATTCGATGGGGAAGAACTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTTTATTGAGTTGCCGc                                                                                                                                          <  2:3256681/150‑1 (MQ=255)
             gTATTCGATGGGGAAGAACTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTTTATTGAGTTGCCGc                                                                                                                                          <  2:627385/150‑1 (MQ=255)
              tATTCGATGGGGAAGAACTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATTTGTTAAAAAAGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTTCCGCt                                                                                                                                         >  1:748661/1‑150 (MQ=255)
              tATTCGATGGGGAAGAACTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCt                                                                                                                                         >  2:2724274/1‑150 (MQ=255)
                ttCGATGGGGAAGAACTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGTTGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAATTGCGGCAACTCAATAAAGTTTCCGCttt                                                                                                                                       >  1:674507/1‑150 (MQ=255)
                ttCGATGGGGAAGAACTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTTTATTGAGTTGCCGCttt                                                                                                                                       <  2:2219988/150‑1 (MQ=255)
                  cGATGGGGAAGAACTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTAc                                                                                                                                     >  1:2375778/1‑150 (MQ=255)
                  cGATGGGGAAGAACTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTAc                                                                                                                                     >  2:888132/1‑150 (MQ=255)
                  cGATGGGGAAGAACTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCGGTAGCAGAATGAAGCGCGGAATAAAAAATCGGCAACTCAATAAAGTTGCCGCTTTAc                                                                                                                                     >  1:1440785/1‑150 (MQ=255)
                            gaaCTTCACGCGAGCTATTATCAGTCTTTATTATCTCAGGTACGGTATTTGGTAGATGCAATATATGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTa                                                                                                                           <  1:1717146/150‑1 (MQ=255)
                            gaaCTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTa                                                                                                                           <  1:3831160/150‑1 (MQ=255)
                            gaaCTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGCGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCAGTAGCAGAATGAAGAGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTa                                                                                                                           >  2:77065/1‑150 (MQ=255)
                              aCTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGa                                                                                                                         <  2:4158861/150‑1 (MQ=255)
                              aCTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGa                                                                                                                         >  2:3869448/1‑150 (MQ=255)
                               cTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGaa                                                                                                                        <  2:2297361/150‑1 (MQ=255)
                                ttCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAAc                                                                                                                       >  2:3955385/1‑150 (MQ=255)
                                ttCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAAc                                                                                                                       >  2:4230016/1‑150 (MQ=255)
                                ttCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGAACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAAc                                                                                                                       >  1:3456230/1‑150 (MQ=255)
                                ttCACGCGAGCTATTATCAGTCGTTATTATCTAAGGTACCGGATATGGTAGATGCACGATAAGATTTTTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACACAAAAAAGTTTCCGCATTACGGGGAAAATAGAAc                                                                                                                       >  1:1038944/1‑150 (MQ=255)
                                ttCACGCGAGCTATTATCAGTCGTTATTATATCAGGTACGGGATATTGTAGATGCACAATAAGATGTTTTTAAAAAGCTGTAGCAGAATTAAGCGCGGAATAAAAAATCGGCAACTCAAAAAAGTTTCCGCTTTAAGGGGAAATTAGaaa                                                                                                                       >  1:374842/1‑149 (MQ=255)
                                   tcgcgAGCTATTATAAGTCGTTATTATCTCAGGTACGGTATATGGTAGATTCACTATAAGATTTGTTAAAAACGATGTAGCAGAATGAAGCGCGGATTAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACAtt                                                                                                                    <  1:2239773/149‑1 (MQ=255)
                                   aCGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGGTATGGTAGGTGCACAATAATATGTGTTAAAAAAGCTGTAGCAGAAAGAAGCGCGGAATAAAAAATCGGCAACACAATAAAGTTGCCGCTTTAAGGGGAAAATATaaaaat                                                                                                                    >  1:841934/1‑146 (MQ=255)
                                   aCGCGAGCTATTATCAGTCGTGATTATCTCAGGGACGGGATATGGGAGATGCACAATAAGATTTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCCACACAATAAACTTTCCGCTTTAAGGGGAACTTAGAACaat                                                                                                                    >  1:1253654/1‑148 (MQ=255)
                                      cgAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTTGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTAcc                                                                                                                 <  1:1429282/150‑1 (MQ=255)
                                           tattatCAGTCGTTATTATCTCAGGTACGGGATATTGTAGATGCACTATAAGAAGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTAACGGGGAAATTAGAACAATACCTTATg                                                                                                            >  2:3250462/1‑150 (MQ=255)
                                            attatCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGa                                                                                                           <  1:1932471/150‑1 (MQ=255)
                                                tCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGt                                                                                                       >  2:92639/1‑150 (MQ=255)
                                                    tCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTg                                                                                                   <  2:3496862/150‑1 (MQ=255)
                                                       ttattaTCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTc                                                                                                <  1:2333402/150‑1 (MQ=255)
                                                       ttattaTCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTc                                                                                                >  1:3054610/1‑150 (MQ=255)
                                                       ttattaTCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTc                                                                                                >  1:552947/1‑150 (MQ=255)
                                                        tattatCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAACCGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCa                                                                                               <  1:2706784/150‑1 (MQ=255)
                                                         attatCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCaa                                                                                              >  2:3982869/1‑150 (MQ=255)
                                                          ttatCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAg                                                                                             <  1:3208139/150‑1 (MQ=255)
                                                              ctcAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATg                                                                                         <  1:187704/150‑1 (MQ=255)
                                                               tcAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGc                                                                                        >  2:3016387/1‑150 (MQ=255)
                                                               tcAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGc                                                                                        >  2:4009635/1‑150 (MQ=255)
                                                               tcAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGc                                                                                        >  1:212195/1‑150 (MQ=255)
                                                               tcAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGc                                                                                        >  1:2872010/1‑150 (MQ=255)
                                                               tcAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGc                                                                                        <  1:706698/150‑1 (MQ=255)
                                                                 aGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCt                                                                                      <  2:3541582/150‑1 (MQ=255)
                                                                 aGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCt                                                                                      >  2:3418528/1‑150 (MQ=255)
                                                                    tACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAATCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTc                                                                                   >  1:2313247/1‑150 (MQ=255)
                                                                     aCGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCa                                                                                  >  2:2182540/1‑150 (MQ=255)
                                                                              ggTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCa                                                                         <  1:3311143/150‑1 (MQ=255)
                                                                              ggTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCa                                                                         <  1:2845101/150‑1 (MQ=255)
                                                                               gTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCAt                                                                        >  1:2227493/1‑150 (MQ=255)
                                                                                     gCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGtctc                                                                  >  1:3590863/1‑150 (MQ=255)
                                                                                      cACTATAAGATGTGTTAAAAACGCTGTAGAAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTctct                                                                 <  2:726883/150‑1 (MQ=255)
                                                                                      cACTATAAGATGTGATAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCATTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTctct                                                                 <  1:130481/150‑1 (MQ=255)
                                                                                             aGATGTGTTAAAAACGCTTTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCg                                                          <  2:3914397/150‑1 (MQ=255)
                                                                                             aGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCACCTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCg                                                          >  2:1519044/1‑150 (MQ=255)
                                                                                             aGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCg                                                          >  2:532687/1‑150 (MQ=255)
                                                                                              gATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCgg                                                         >  2:298840/1‑150 (MQ=255)
                                                                                               aTGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCggt                                                        <  2:3773648/150‑1 (MQ=255)
                                                                                               aTGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCggt                                                        <  1:2470134/150‑1 (MQ=255)
                                                                                               aTGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCggt                                                        >  1:975428/1‑150 (MQ=255)
                                                                                                   gtTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACAGTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGtt                                                    <  1:4167430/150‑1 (MQ=255)
                                                                                                    ttAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGttt                                                   >  2:1181073/1‑150 (MQ=255)
                                                                                                    ttAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGttt                                                   >  1:917384/1‑150 (MQ=255)
                                                                                                    ttAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGttt                                                   >  2:2344974/1‑150 (MQ=255)
                                                                                                    ttAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTACTTCGGTGGttt                                                   >  2:3308964/1‑150 (MQ=255)
                                                                                                     tAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTa                                                  <  2:248834/150‑1 (MQ=255)
                                                                                                      aaaaaCGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTaa                                                 <  1:2532430/150‑1 (MQ=255)
                                                                                                                  gCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAACTTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGtt                                     >  2:1331277/1‑150 (MQ=255)
                                                                                                                  gCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGtt                                     >  1:3008424/1‑150 (MQ=255)
                                                                                                                  gCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGtt                                     <  2:2827607/150‑1 (MQ=255)
                                                                                                                   cAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGttt                                    >  2:2025719/1‑150 (MQ=255)
                                                                                                                     gAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAATTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGt                                  <  2:952872/150‑1 (MQ=255)
                                                                                                                           aGCGCGGAATAAAAAAGCGGCAACTCAATACAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCtt                            <  2:464832/150‑1 (MQ=255)
                                                                                                                           aGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCtt                            <  2:3501172/150‑1 (MQ=255)
                                                                                                                            gcgcGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTc                           <  1:3869448/150‑1 (MQ=255)
                                                                                                                             cgcgGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCa                          <  2:3194310/150‑1 (MQ=255)
                                                                                                                                   aTAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCAtt                    <  1:1198158/150‑1 (MQ=255)
                                                                                                                                    ccgtaaaGCGGCAACTTTATTGAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTg                   >  2:363819/5‑150 (MQ=255)
                                                                                                                                    ccgtaaaGCGGCAACTTTATTGAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTg                   >  2:12451/5‑150 (MQ=255)
                                                                                                                                     aaaaaaGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACAGTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGc                  <  2:2768644/150‑1 (MQ=255)
                                                                                                                                         aaGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCAcc              <  1:1569833/150‑1 (MQ=255)
                                                                                                                                          aGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCAccc             >  2:3608294/1‑150 (MQ=255)
                                                                                                                                          aGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCAccc             >  2:3202296/1‑150 (MQ=255)
                                                                                                                                           gCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTGTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGACACCCa            >  2:3135267/1‑150 (MQ=255)
                                                                                                                                              gCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCAttt         >  1:117045/1‑150 (MQ=255)
                                                                                                                                              gCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCAGTGCCACCCAttt         <  2:2130649/150‑1 (MQ=255)
                                                                                                                                                aaCTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCATgtgt       >  2:1535511/1‑150 (MQ=255)
                                                                                                                                                aaCTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCATTtgt       >  2:1653355/1‑150 (MQ=255)
                                                                                                                                                aaCTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCATTtgt       >  2:1964996/1‑150 (MQ=255)
                                                                                                                                                  cTCAATAAAGTTGCCGCTTGACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCATTTgtgt     <  1:4043700/150‑1 (MQ=255)
                                                                                                                                                   tCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCATTTGTGtt    >  2:3420747/1‑150 (MQ=255)
                                                                                                                                                   tCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCATTTGTGtt    <  1:1217146/150‑1 (MQ=255)
                                                                                                                                                    cAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCATTTGTGttt   <  2:2242450/150‑1 (MQ=255)
                                                                                                                                                     aaTAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCATTTGTGTTTg  >  1:166049/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GTGTAATACAGTGGTATTCGATGGGGAAGAACTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCATTTGTGTTTG  >  NC_000913/950165‑950463

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: