Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 950,318 A→G 5.7% intergenic (+60/+22) ycaK → / ← pflA putative NAD(P)H‑dependent oxidoreductase/pyruvate formate‑lyase 1‑activating enzyme; [formate‑C‑acetyltransferase 1]‑activating enzyme; PFL activase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913950,3180AG 5.7% 764.0 / 24.1 229intergenic (+60/+22)ycaK/pflAputative NAD(P)H‑dependent oxidoreductase/pyruvate formate‑lyase 1‑activating enzyme; [formate‑C‑acetyltransferase 1]‑activating enzyme; PFL activase
Reads supporting (aligned to +/- strand):  ref base A (123/92);  new base G (4/9);  total (127/102)
Fisher's exact test for biased strand distribution p-value = 8.44e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.61e-01

Only 100 of 234 total aligned reads displayed.

ATACAGTGGTATTCGATGGGGAAGAACTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCATTTGTGTTTGCCCA  >  NC_000913/950170‑950467
                                                                                                                                                    |                                                                                                                                                      
aTACAGTGGTATTCGATGGGGAAGAACTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATaaa                                                                                                                                                       <  2:1350566/150‑1 (MQ=255)
 tACAGTGGTATTCGATGGGGAAGAACTTCACGCGAGCTATTATCAGTAGTTATTTTATCAGGTACGGGATATTTTAGATGCACTATAACATGTGTTAAAAAAGCTGTAGCAGAATGAATCCCGGAATAAAAAATCGGCAACACAAAAAAg                                                                                                                                                      >  1:1202966/1‑150 (MQ=255)
   ctgTGGTATTGGTTGGGTAAGAACTTCACGCTAGCTATTATCAGTCGTTATTATCTCAGGTACGGTATATGGTAGATGCACTATAAGTTGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGtt                                                                                                                                                    <  1:2613056/148‑1 (MQ=255)
   ctgTGGTATTCGTTGGGTAATAACTTCACGCGAGATATTATCAGTCTTTATTATCTCTGGTACGGTATTTGTTAGTTGAACTATATGTTGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGTAATAAAAAAGCGGCAACTCAATAAAGtt                                                                                                                                                    <  1:4011122/148‑1 (MQ=255)
      tGGTATTCGATGGGGAAGAACTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGcc                                                                                                                                                 >  1:3914369/1‑150 (MQ=255)
        gTATTCGATGGGGAAGAACTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTTTATTGAGTTGCCGc                                                                                                                                               <  1:131317/150‑1 (MQ=255)
        gTATTCGATGGGGAAGAACTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTTTATTGAGTTGCCGc                                                                                                                                               <  2:3256681/150‑1 (MQ=255)
        gTATTCGATGGGGAAGAACTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTTTATTGAGTTGCCGc                                                                                                                                               <  2:627385/150‑1 (MQ=255)
         tATTCGATGGGGAAGAACTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCt                                                                                                                                              >  2:2724274/1‑150 (MQ=255)
         tATTCGATGGGGAAGAACTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCGGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCt                                                                                                                                              >  2:1279351/1‑150 (MQ=255)
           ttCGATGGGGAAGAACTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTTTATTGAGTTGCCGCttt                                                                                                                                            <  2:2219988/150‑1 (MQ=255)
             cGATGGGGAAGAACTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTAc                                                                                                                                          >  2:888132/1‑150 (MQ=255)
             cGATGGGGAAGAACTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTAc                                                                                                                                          >  1:2375778/1‑150 (MQ=255)
                     aagaaCTTCACGCGAGCTATTATCAGTCGTGATTATCTCAGGTACGGGCTATGGTAGATGCACAATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAAt                                                                                                                                 >  1:3592136/1‑150 (MQ=255)
                       gaaCTTCACGCGAGCTATTATCAGTCTTTATTATCTCAGGTACGGTATTTGGTAGATGCAATATATGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTa                                                                                                                               <  1:1717146/150‑1 (MQ=255)
                       gaaCTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTa                                                                                                                               <  1:3831160/150‑1 (MQ=255)
                       gaaCTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTa                                                                                                                               >  2:3402762/1‑150 (MQ=255)
                       gaaCTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGCGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCAGTAGCAGAATGAAGAGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTa                                                                                                                               >  2:77065/1‑150 (MQ=255)
                         aCTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGa                                                                                                                             >  2:3869448/1‑150 (MQ=255)
                         aCTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGa                                                                                                                             <  2:4158861/150‑1 (MQ=255)
                          cTTCACTCTAGCTATTATCTGTCGTTATTATCTCAGGTACGGGATATGGTAGATGAACTATAAGATGTGTTATAAACGCTGTATCTTATTGAAGCGCGGAATAATAAAGAGGCTACTCAATAAAGTTGCCGCTTTACGGGTAAATTTGaa                                                                                                                            <  2:3598737/150‑1 (MQ=255)
                          cTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGaa                                                                                                                            <  2:2297361/150‑1 (MQ=255)
                           ttCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTTCGGGATATGGTAGATGCACGATAATATTTTTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAACGGCAACACAATAAAGTTGACGCTATACTGGGGAATTAGAAc                                                                                                                           >  1:680428/1‑150 (MQ=255)
                           ttCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGACGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAAc                                                                                                                           >  2:3955397/1‑150 (MQ=255)
                           ttCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAAc                                                                                                                           >  2:48471/1‑150 (MQ=255)
                           ttCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAAc                                                                                                                           >  1:2787197/1‑150 (MQ=255)
                           ttCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAAc                                                                                                                           >  2:4230016/1‑150 (MQ=255)
                           ttCACGCGAGCTATTATCAGTCGTTATTATATCAGGTACGGGATATGGTAGATGCACAATAAGATGTGTTAAAAAAGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGaaa                                                                                                                           >  1:3037522/1‑149 (MQ=255)
                              tcgcgAGCTATTATAAGTCGTTATTATCTCAGGTACGGTATATGGTAGATTCACTATAAGATTTGTTAAAAACGATGTAGCAGAATGAAGCGCGGATTAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACAtt                                                                                                                        <  1:2239773/149‑1 (MQ=255)
                              aCGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATTTTTTAAAAACCCGGTAGCAGAAAGAAGCGCGGAATAAAAAATCGGCAACACAATAAAGTTTCCGCTTTAAGGGGAAAATAGAACaat                                                                                                                        >  1:1253647/1‑148 (MQ=255)
                              aCGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACAtt                                                                                                                        <  1:80582/150‑1 (MQ=255)
                                     cTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTAt                                                                                                                 <  2:1210284/150‑1 (MQ=255)
                                       attatCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGa                                                                                                               <  1:1932471/150‑1 (MQ=255)
                                           tCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGt                                                                                                           >  2:92639/1‑150 (MQ=255)
                                               tCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTg                                                                                                       <  2:3496893/150‑1 (MQ=255)
                                               tCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTg                                                                                                       <  1:2454540/150‑1 (MQ=255)
                                                  ttattaTCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTc                                                                                                    <  1:2333402/150‑1 (MQ=255)
                                                   tattatCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAACCGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCa                                                                                                   <  1:2706784/150‑1 (MQ=255)
                                                    attatCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCaa                                                                                                  >  2:3982869/1‑150 (MQ=255)
                                                         ctcAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATg                                                                                             <  1:1500498/150‑1 (MQ=255)
                                                         ctcAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATg                                                                                             <  1:187704/150‑1 (MQ=255)
                                                          tcAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCTAGAATGc                                                                                            >  1:957124/1‑150 (MQ=255)
                                                          tcAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGc                                                                                            >  2:3016387/1‑150 (MQ=255)
                                                          tcAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGc                                                                                            >  2:3295420/1‑150 (MQ=255)
                                                          tcAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGc                                                                                            >  2:4009635/1‑150 (MQ=255)
                                                            aGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCt                                                                                          <  2:3541582/150‑1 (MQ=255)
                                                               tACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTc                                                                                       >  1:2642962/1‑150 (MQ=255)
                                                                aCGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCa                                                                                      >  2:2182537/1‑150 (MQ=255)
                                                                      tatGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGtt                                                                                <  1:3955397/150‑1 (MQ=255)
                                                                         ggTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCa                                                                             <  1:3311143/150‑1 (MQ=255)
                                                                          gTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCAt                                                                            >  1:747176/1‑150 (MQ=255)
                                                                          gTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCAt                                                                            >  1:2227493/1‑150 (MQ=255)
                                                                                 cACTATAAGATGTGTTAAAAACGCTGTAGAAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTctct                                                                     <  2:726883/150‑1 (MQ=255)
                                                                                   cTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCtcttt                                                                   <  2:4060941/150‑1 (MQ=255)
                                                                                   cTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCtcttt                                                                   >  1:2845590/1‑150 (MQ=255)
                                                                                     ataAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGGTCCATGGTCtctttct                                                                 >  1:1255891/1‑150 (MQ=255)
                                                                                        aGATGTGTTAAAAACGCTTTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCg                                                              <  2:3914397/150‑1 (MQ=255)
                                                                                        aGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCACCTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCg                                                              >  2:1519044/1‑150 (MQ=255)
                                                                                        aGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCg                                                              >  2:532687/1‑150 (MQ=255)
                                                                                         gATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCgg                                                             >  2:298840/1‑150 (MQ=255)
                                                                                          aTGTGTTAAAACCGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCggt                                                            <  1:3711076/150‑1 (MQ=255)
                                                                                            gtgtTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATTCCTTTCACGCGTTCCATGGTCTCTTTCTTCggtgg                                                          <  2:3458336/150‑1 (MQ=255)
                                                                                             tgtTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTACTTCggtggt                                                         >  2:3213174/1‑150 (MQ=255)
                                                                                              gtTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAATGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGtt                                                        >  1:143737/1‑150 (MQ=255)
                                                                                               ttAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGttt                                                       >  1:1098475/1‑150 (MQ=255)
                                                                                               ttAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGttt                                                       >  2:2344974/1‑150 (MQ=255)
                                                                                               ttAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGttt                                                       >  1:345057/1‑150 (MQ=255)
                                                                                               ttAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGttt                                                       >  1:844753/1‑150 (MQ=255)
                                                                                                 aaaaaCGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTaa                                                     <  1:2532430/150‑1 (MQ=255)
                                                                                                  aaaaCGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAac                                                    >  1:597248/1‑150 (MQ=255)
                                                                                                        cTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTc                                              <  2:3831727/150‑1 (MQ=255)
                                                                                                             gCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGtt                                         >  1:3008424/1‑150 (MQ=255)
                                                                                                              cAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGttt                                        >  2:2025719/1‑150 (MQ=255)
                                                                                                                gAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAATTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGt                                      <  2:952872/150‑1 (MQ=255)
                                                                                                                     aaGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTActct                                 >  2:2806432/1‑150 (MQ=255)
                                                                                                                      aGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTTCTCtt                                >  1:1177226/1‑150 (MQ=255)
                                                                                                                        cgcgGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCa                              <  2:3194310/150‑1 (MQ=255)
                                                                                                                         gcgGAATTAAAAACCGGCAACTCAATAAAGTTGGCGCTTTTCGGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCa                              <  1:2254822/150‑1 (MQ=255)
                                                                                                                         gcgGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCAc                             <  2:3052685/150‑1 (MQ=255)
                                                                                                                          cgGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCAcc                            >  1:4212922/1‑150 (MQ=255)
                                                                                                                               ccgtaaaGCGGCAACTTTATTGAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTg                       >  2:363819/5‑150 (MQ=255)
                                                                                                                                aaaaaaGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACAGTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGc                      <  2:2768644/150‑1 (MQ=255)
                                                                                                                                  aaaaGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCa                    <  1:1027690/150‑1 (MQ=255)
                                                                                                                                  aaaaGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCa                    >  1:2066297/1‑150 (MQ=255)
                                                                                                                                    aaGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCAcc                  <  1:1569833/150‑1 (MQ=255)
                                                                                                                                     aGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCAccc                 >  2:3202296/1‑150 (MQ=255)
                                                                                                                                        ggCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCAtt              <  1:3861317/150‑1 (MQ=255)
                                                                                                                                          cAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCATTtg            >  2:2427955/1‑150 (MQ=255)
                                                                                                                                           aaCTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCATgtgt           >  2:1535511/1‑150 (MQ=255)
                                                                                                                                           aaCTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCATTtgt           >  2:1653355/1‑150 (MQ=255)
                                                                                                                                           aaCTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCATTtgt           >  2:1964996/1‑150 (MQ=255)
                                                                                                                                             cTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCATTTgtgt         <  1:3456236/150‑1 (MQ=255)
                                                                                                                                             cTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCATTTgtgt         >  1:3093577/1‑150 (MQ=255)
                                                                                                                                             cTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCATTTgtgt         <  2:1098475/150‑1 (MQ=255)
                                                                                                                                             cTCAATAAAGTTGCCGCTTGACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCATTTgtgt         <  1:4043700/150‑1 (MQ=255)
                                                                                                                                              tCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCATTTGTGtt        >  2:3420747/1‑150 (MQ=255)
                                                                                                                                               cAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCATTTGTGttt       <  1:3605444/150‑1 (MQ=255)
                                                                                                                                               cAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCATTTGTGttt       <  2:2242450/150‑1 (MQ=255)
                                                                                                                                                aaTAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCATTTGTGTTTg      >  1:166049/1‑150 (MQ=255)
                                                                                                                                                 aTAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCATTTGTGTTTGc     >  2:2081946/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                      
ATACAGTGGTATTCGATGGGGAAGAACTTCACGCGAGCTATTATCAGTCGTTATTATCTCAGGTACGGGATATGGTAGATGCACTATAAGATGTGTTAAAAACGCTGTAGCAGAATGAAGCGCGGAATAAAAAAGCGGCAACTCAATAAAGTTGCCGCTTTACGGGGAAATTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCCTTTCACGCGTTCCATGGTCTCTTTCTTCGGTGGTTTAACACCGTCGAGTTTGTACTCTTCACCCATTGCCACCCATTTGTGTTTGCCCA  >  NC_000913/950170‑950467

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: