BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,389,007	A→C	8.2%	E26D (GAA→GAC)	ycjG →	L‑Ala‑D/L‑Glu epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,389,007	0	A	C	8.2%	580.6 / 8.9	205	E26D (GAA→GAC)	ycjG	L‑Ala‑D/L‑Glu epimerase
Reads supporting (aligned to +/- strand): ref base A (74/110); new base C (19/2); total (93/112)
Fisher's exact test for biased strand distribution p-value = 9.20e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 210 total aligned reads displayed.

GCTAACTGTCAATAACACAGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAGGGTATTAAAGGCACCGGCGAATGCACGCCGTATCCGCGTTATGGGGAAAGTGATGCCTCGGTAATGGCGCAAATTATGAGCGTCGTGCCGCAACTAGAGAAAGGGCTGACACGGGA  >  NC_000913/1388858‑1389156

gCTAACTGTCAATAACACAGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGaa                                                                                                                                                       <  1:3609845/150‑1 (MQ=255)

gCTAACTGTCAATAACACAGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGaa                                                                                                                                                       <  1:1987049/150‑1 (MQ=255)

  tAACTGTCAATAACACAGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGACgc                                                                                                                                                     >  2:1738763/1‑150 (MQ=255)

      tGTCAATAACACAGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGACGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCCGTGACgcgcgc                                                                                                                                                 >  2:2289570/1‑150 (MQ=255)

      tGTCAATAACACAGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAgcgcgc                                                                                                                                                 >  1:439062/1‑150 (MQ=255)

      tGTCAATAACACAGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGGGAAgcgcgc                                                                                                                                                 >  2:1715793/1‑150 (MQ=255)

          aaTAACACAGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAGCGCGCgtgg                                                                                                                                             <  1:1677392/150‑1 (MQ=255)

          aaTAACACAGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAGCGCGCgtgg                                                                                                                                             >  1:3936695/1‑150 (MQ=255)

            tAACACAGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAGCGCGCgtggtg                                                                                                                                           <  1:2395701/150‑1 (MQ=255)

              acacAGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAGCGCGCgtggtggt                                                                                                                                         <  1:1235523/150‑1 (MQ=255)

               cacaGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAGCGCGCgtggtggtg                                                                                                                                        >  1:3057440/1‑150 (MQ=255)

               cacaGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAGCGCGCgtggtggtg                                                                                                                                        <  2:3386876/150‑1 (MQ=255)

                acaGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGACGCGCGCGtggtggtgg                                                                                                                                       >  2:1725777/1‑150 (MQ=255)

                acaGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAGCGCGCGtggtggtgg                                                                                                                                       <  2:3947567/150‑1 (MQ=255)

                 caGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAACGAGTAAAGATGAGAACCGATAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAGCGCGCGTggtggtggt                                                                                                                                      <  2:1930416/150‑1 (MQ=255)

                   gCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAGCGCGCGTGGTGGTGGTTg                                                                                                                                    >  2:2937218/1‑150 (MQ=255)

                        cGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAGCGCGCGTGGTGGTGGTTGAACTg                                                                                                                               <  1:2940309/150‑1 (MQ=255)

                         gCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGACGTCGCAGTGAAGCGCGCGTGGTGGTGGTTGAACTgg                                                                                                                              >  1:3589291/1‑150 (MQ=255)

                          cTATTTGGGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGTAGTCGCAGTGAAGCGCGCGTGGTGGTGGTTGAACTGga                                                                                                                             <  1:2295770/150‑1 (MQ=255)

                          cTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAGCGCGCGTGGTGGTGGTTGAACTGga                                                                                                                             <  1:3775782/150‑1 (MQ=255)

                          cTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGAAGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGTGAAGTCGCAGTGAAGCGCGCGTGGTGGTGGTTGAACTGga                                                                                                                             <  1:1712632/150‑1 (MQ=255)

                           tATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAGCGCGCGTGGTGGTGGTTGAACTGgaa                                                                                                                            >  1:3249985/1‑150 (MQ=255)

                                  gcTTACTCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAgg                                                                                                                     <  2:3587310/150‑1 (MQ=255)

                                  gcTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAgg                                                                                                                     <  1:1966569/150‑1 (MQ=255)

                                  gcTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAgg                                                                                                                     <  2:3543220/150‑1 (MQ=255)

                                  gcTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAgg                                                                                                                     <  1:1342415/150‑1 (MQ=255)

                                  gcTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAgg                                                                                                                     <  2:4192178/150‑1 (MQ=255)

                                   cTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGACGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAggg                                                                                                                    >  2:2607961/1‑150 (MQ=255)

                                   cTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAggg                                                                                                                    >  2:3498685/1‑150 (MQ=255)

                                    ttAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGACGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAGGGt                                                                                                                   >  1:2519165/1‑150 (MQ=255)

                                    ttAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTGTGTGATTGCCCGGGGCAGTCGCAGTGCCGCGCGCGTGGTGGTGGTTGAACTGGACGAAGAGGGt                                                                                                                   >  1:720036/1‑150 (MQ=255)

                                     tAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAGGGTa                                                                                                                  <  1:2757996/150‑1 (MQ=255)

                                        tCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAGGGTATTa                                                                                                               >  2:692192/1‑150 (MQ=255)

                                          cGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGACGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAGGGTATTaaa                                                                                                             >  1:1730092/1‑150 (MQ=255)

                                          cGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAGGGTATTaaa                                                                                                             <  1:1639345/150‑1 (MQ=255)

                                                 caccGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAGGGTATTAAAGGCACCg                                                                                                      <  2:2896769/150‑1 (MQ=255)

                                                  accGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAGGGTATTAAAGGCACCgg                                                                                                     >  2:1045791/1‑150 (MQ=255)

                                                   ccGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAGGGTATTAAAGGCACCGGc                                                                                                    >  1:3209230/1‑150 (MQ=255)

                                                           acaaGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAGGGTATTAAAGGCACCGGCGAATGCAc                                                                                            <  2:165133/150‑1 (MQ=255)

                                                           acaaGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAGGGTATTAAAGGCACCGGCGAATGCAc                                                                                            <  2:2311965/150‑1 (MQ=255)

                                                             aaGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGCAGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAGGGTATTAAAGGCACCGGCGAATGCACGc                                                                                          >  2:2163301/1‑150 (MQ=255)

                                                              atgAGTAAATATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCACTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCTCAGTGAAGCGCGCGTGGTTGTGGTTTAACTGGAAGAAGATGGTATTAAAGGCACCGGCGAATGCACGcc                                                                                         <  1:3287560/148‑1 (MQ=255)

                                                              aGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAGGGTATTAAAGGCACCGGCGAATGCACGcc                                                                                         <  1:788871/150‑1 (MQ=255)

                                                                 aGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAGGGTATTAAAGGCACCGGCGAATGCACGCCGTa                                                                                      >  1:303168/1‑150 (MQ=255)

                                                                   tAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCACGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAGGGTATTAAAGGCACCGGCGAATGCACGCCGTATc                                                                                    >  2:1549038/1‑150 (MQ=255)

                                                                      aGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAGGGTATTAAAGGCACCGGCGAATGCACGCCGTATCcgc                                                                                 <  1:3317842/150‑1 (MQ=255)

                                                                      aGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAGGGTATTAAAGGCACCGGCGAATGCACGCCGTATCcgc                                                                                 <  1:3317880/150‑1 (MQ=255)

                                                                          gagaACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGACGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAGGGTATTAAAGGCACCGGCGAATGCACGCCGTATCCGCGTTa                                                                             >  2:4183027/1‑150 (MQ=255)

                                                                          gagaACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAGGGTATTAAAGGCACCGGCGAATGCACGCCGTATCCGCGTTa                                                                             <  1:2352673/150‑1 (MQ=255)

                                                                             aaCCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAGGGTATTAAAGGCACCGGCGAATGCACGCCGTATCCGCGTTATgg                                                                          >  2:2786116/1‑150 (MQ=255)

                                                                                  ttAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCTGTGAAGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAGGGTATTAATGGCACCGGCGATTGCACGCCGTATCCGCGTTATGGGGaaa                                                                     >  1:214086/1‑150 (MQ=255)

                                                                                  ttAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAGGGTATTAAAGGCACCGGCGAATGCACGCCGTATCCGCGTTATGGGGaaa                                                                     <  1:1875091/150‑1 (MQ=255)

                                                                                    aaGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAGGGTATTAAAGGCACCGGCGAATGCACGCCGTATCCGCGTTATGGGGAAAGt                                                                   <  2:1638062/150‑1 (MQ=255)

                                                                                     aGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAGGGTATTAAAGGCACCGGCGAATGCACGCCGTATCCGCGTTATGGGGAAAGTg                                                                  <  2:3397019/150‑1 (MQ=255)

                                                                                        tATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAGGGTATTAAAGGCACCGGCGAATGCACGCCGTATCCGCGTTATGGGGAAAGTGATg                                                               <  2:3877246/150‑1 (MQ=255)

                                                                                         aTTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAGGGTATTAAAGGCACCGGCGACTGCACGCCGTATCCGCGTTATGGGGACAGTGATGc                                                              >  2:2850739/1‑150 (MQ=255)

                                                                                         aTTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAGGGTATTAAAGGCACCGGCGAATGCACGCCGTATCCGCGTTATGGGGAAAGTGATGc                                                              >  2:4032573/1‑150 (MQ=255)

                                                                                         aTTCGAGGAAGCCTGGCCCTTACATACCCCGATTGTGATTGCCCGGGGAAGTCGCAGTGAAGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAGGCTATTAAAGGCACCGGCGAATGCACGCCGTATCCGCGTTATGGGGAAAGTGATGc                                                              <  1:2902167/150‑1 (MQ=255)

                                                                                              aGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGACGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAGGGTATTAAAGGCACCGGCGAATGCACGCCGTATCCGCGTTATGGGGAAAGTGATGCCTCgg                                                         >  2:3534789/1‑150 (MQ=255)

                                                                                                gAAGCCTGGCCCTTACATACCCCGTTTGTGGTTGCCCGGGGAAGTCGCAGTGAAGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAGGGTATTAAAGGCACCGGCGAATGCACGCCGTATCCGCGTTATGGGGAAAGTGATGCCTCGGTa                                                       >  1:202134/1‑150 (MQ=255)

                                                                                                gAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAGGGTATTAAATGCACCGGCGAATGCACGCCGTATCCGCGTTATGGGGAAAGTGATGCCTCGGTa                                                       >  1:957448/1‑150 (MQ=255)

                                                                                                gAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAGGGTATTAAAGGCACCGGCGAATGCACGCCGTATCCGCGTTATGGGGAAAGTGATGCCTCGGTa                                                       >  1:3684678/1‑150 (MQ=255)

                                                                                                  aGCCTGGCCCTTACAGACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAGGGTATTAAAGGCACCGGCGAATGCACGCCGTATCCGCGTTATGGGGAAAGTGATGCCTCGGTAAt                                                     <  1:637428/150‑1 (MQ=255)

                                                                                                   gCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAGGGTATTAAAGGCACCGGCGAATGCACGCCGTATCCGCGTTATGGGGAAAGTGATGCCTCGGTAATg                                                    <  1:538199/150‑1 (MQ=255)

                                                                                                   gCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAGGGTATTAAAGGCACCGGCGAATGCACGCCGTATCCGCGTTATGGGGAAAGTGATGCCTCGGTAATg                                                    <  1:1210769/150‑1 (MQ=255)

                                                                                                     cTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAGGGTATTAAAGGCACCGGCGAATGCACGCCGTATCCGCGTTATGGGGAAAGTGATGCCTCGGTAATGgc                                                  <  2:556194/150‑1 (MQ=255)

                                                                                                          ccTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAGGGTATTAAAGGCACCGGCGAATGCACGCCGTATCCGCGTTATGGGGAAAGTGATGCCTCGGTAATGGCGCaaa                                             >  1:2691937/1‑150 (MQ=255)

                                                                                                           cTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAGGGTATTAAAGGCACCGGCGAATGCACGCCGTATCCGCGTTATGGGGAAAGTGATGCCTCGGTAATGGCGCAAAt                                            >  1:1783405/1‑150 (MQ=255)

                                                                                                           cTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAGGGTATTAAAGGCACCGGCGAATGCACGCCGTATCCGCGTTATGGGGAAAGTGATGCCTCGGTAATGGCGCAAAt                                            >  1:3458108/1‑150 (MQ=255)

                                                                                                             tACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAGGGTATTAAAGGCACCGGCGAATGCACGCCGTATCCGCGTTATGGGGAAAGTGATGCCTCGGTAATGGCGCAAATTa                                          >  1:1128309/1‑150 (MQ=255)

                                                                                                              aCATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGACGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAGGGTATTAAAGGCACCGGCGAATGCACGCCGTATCCGCGTTATGGGGAAAGTGATGCCTCGGTAATGGCGCAAATTAt                                         >  1:1382036/1‑150 (MQ=255)

                                                                                                               cATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAGGGTATTAAAGGCACCGGCGAATGCACGCCGTATCCGCGTTATGGGGAAAGTGATGCCTCGGTAATGGCGCAAATTATg                                        >  2:719419/1‑150 (MQ=255)

                                                                                                                aTACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGACGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAGGGTATTAAAGGCACCGGCGAATGCACGCCGTATCCGCGTTATGGGGAAAGTGATGCCTCGGTAATGGCGCAAATTATGa                                       >  1:1153363/1‑150 (MQ=255)

                                                                                                                aTACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAGGGTATTAAAGGCACCGGCGAATGCACGCCGTATCCGCGTTATGGGGAAAGTGATGCCTCGGTAATGGCGCAAATTATGa                                       <  1:1095147/150‑1 (MQ=255)

                                                                                                                aTACCCCGTTGGTGATTGCCCGGGGAAGTCGCAGTGAAGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAGGGTATTAAAGGCACCGGCGAATGCACGCCGTATCCGCGTTATGGGGAAAGTGATGCCTCGGTAATGGCGCAAATTATGa                                       <  2:538804/150‑1 (MQ=255)

                                                                                                                  aCCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAGGGTATTAAAGGCACCGGCGAATGCACGCCGTATCCGCGTTATGGGGAAAGTGATGCCTCGGTAATGGCGCAAATTATGAGc                                     >  1:1841918/1‑150 (MQ=255)

                                                                                                                   ccccGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAGGGTATTAAAGGCACCGGCGAATGCACGCCGTATCCGCGTTATGGGGAAAGTGATGCCTCGGTAATGGCGCAAATTATGAGcg                                    <  2:2883987/150‑1 (MQ=255)

                                                                                                                    cccGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAGGGTATTAAAGGCACCGGCGAATGCACGCCGTATCCGCGTTATGGGGAAAGTGATGCCTCGGTAATGGCGCAAATTATGAGcgt                                   >  1:2172315/1‑150 (MQ=255)

                                                                                                                          tgtgATTGCCCGGGGAAGTCGCAGTGACGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAGGGTATTAAAGGCACCGGCGAATGCACGCCGTATCCGCGTTATGGGGAAAGTGATGCCTCGGTAATGGCGCAAATTATGAGCGTCGTGcc                             >  2:1601022/1‑150 (MQ=255)

                                                                                                                          tgtgATTGCCCGGGGAAGTCGCAGTGAAGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAGGGTATTAAAGGCACCGGCGAATGCACGCCGTATCCGCGTTATGGGGAAAGTGATGCCTCGGTAATGGCGCAAATTATGAGCGTCGTGcc                             <  1:3125458/150‑1 (MQ=255)

                                                                                                                           gtgATTGCCCGGGGAAGTCGCAGTGAAGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAGGGTATTAAAGGCACCGGCGAATGCACGCCGTATCCGCGTTATGGGGAAAGTGATGCCTCGGTAATGGCGCAAATTATGAGCGTCGTGCCg                            >  1:503533/1‑150 (MQ=255)

                                                                                                                            tgATTGCCCGGGGAAGTCGCAGTGAAGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAGGGTATTAAAGGCACCGGCGAATGCACGCCGTATCCGCGTTATGGGGAAAGTGATGCCTCGGTAATGGCGCAAATTATGAGCGTCGTGCCGc                           <  1:2644730/150‑1 (MQ=255)

                                                                                                                            tgATTGCCCGGGGAAGTCGCAGTGAAGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAGGGTATTAAAGGCACCGGCGAATGCACGCCGTATCCGCGTTATGGGGAAAGTGATGCCTCGGTAATGGCGCAAATTATGAGCGTCGTGCCGc                           <  1:2054409/150‑1 (MQ=255)

                                                                                                                            tgATTGCCCGGGGAAGTCGCAGTGAAGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAGGGTATTAAAGGCACCGGCGAATGCACGCCGTATCCGCGTTATGGGGAAAGTGATGCCTCGGTAATGGCGCAAATTATGAGCGTCGTGCCGc                           <  1:2054506/150‑1 (MQ=255)

                                                                                                                              aTTGCCCGGGGAAGTCGCAGTGAAGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAGGGTATTAAAGGCACCGGCGAATGCACGCCGTATCCGCGTTATGGGGAAAGTGATGCCTCGGTAATGGCGCAAATTATGAGCGTCGTGCCGCaa                         >  1:2387021/1‑150 (MQ=255)

                                                                                                                                   ccGGGGAAGTCGCAGTGAAGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAGGGTATTAAAGGCACCGGCGAATGCACGCCGTATCCGCGTTATGGGGAAAGTGATGCCTCGGTAATGGCGCAAATTATGAGCGTCGTGCCGCAACTaga                    <  2:1427336/150‑1 (MQ=255)

                                                                                                                                   ccGGGGAAGTCGCAGTGAAGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAGGGTATTAAAGGCACCGGCGAATGCACGCCGTATCCGCGTTATGGGGAAAGTGATGCCTCGGTAATGGCGCAAATTATGAGCGTCGTGCCGCAACTaga                    <  1:1115721/150‑1 (MQ=255)

                                                                                                                                    cGGGGAAGTCGCAGTGACGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAGGGTATTAAAGGCACCGGCGAATGCACGCCGTATCCGCGGTATGGGGAAAGTGATGCCTCGGTAATGGCGCAAATTATGAGCGTCGTGCCGCAACTagag                   >  2:3806778/1‑150 (MQ=255)

                                                                                                                                    cGGGGAAGTCGCAGTGAAGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAGGGTATTAAAGGCACCGGCGAATGCACGCCGTATCCGCGTTATGGGGAAAGTGATGCCTCGGTAATGGCGCAAATTATGAGCGTCGTGCCGCACCTagag                   >  1:2870945/1‑150 (MQ=255)

                                                                                                                                     ggggAAGTCGCAGTGCAGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAGGGTATTAAAGGCACCGGCGAATGCACGCCGTATCCGCGTTATGGGGAAAGTGATGCCTCGGTAATGGCGCAAATTATGAGCGTCGTGCCGCAACTAgaga                  >  2:1219151/1‑150 (MQ=255)

                                                                                                                                      gggAAGTCGCAGTGAAGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAGGGTATTAAAGGCACCGGCGAATGCACGCCGTATCCGCGTTATGGGGAAAGTGATGCCTCGGTAATGGCGCAAATTATGAGCGTCGTGCCGCAACTAGAGaa                 >  2:1463559/1‑150 (MQ=255)

                                                                                                                                        gAAGTCGCAGTGAAGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAGGGTATTAAAGGCACCGGCGAATGCACGCCGTATCCGCGTTATGGGGAAAGTGATGCCTCGGTAATGGCGCAAATTATGAGCGTCGTGCCGCAACTAGAGAAAg               <  2:3052987/150‑1 (MQ=255)

                                                                                                                                         aaGTCGCAGTGAAGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAGGGTATTAAAGGCACCGGCGAATGCACGCCGTATCCGCGTTATGGGGAAAGTGATGCCTCGGTAATGGCGCAAATTATGAGCGTCGTGCCGCAACTAGAGAAAgg              <  1:992613/150‑1 (MQ=255)

                                                                                                                                         aaGTCGCAGTGAAGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAGGGTATTAAAGGCACCGGCGAATGCACGCCGTATCCGCGTTATGGGGAAAGTGATGCCTCGGTAATGGCGCAAATTATGAGCGTCGTGCCGCAACTAGAGAAAgg              <  1:1275546/150‑1 (MQ=255)

                                                                                                                                          aGTCGCAGTGAAGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAGGGTATTAAAGGCACCGGCGAATGCACGCCGTATCCGCGTTATGGGGAAAGTGATGCCTCGGTAATGGCGCAAATTATGAGCGTCGTGCCGCAACTAGAGAAAggg             <  1:871704/150‑1 (MQ=255)

                                                                                                                                            tCGCAGTGGAGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAGGGTATTAAAGGCACCGGCGAATGCACGCCGTATCCGCGTTATGGGGAAAGTGATGCCTCGGTAATGGCGCAAATTATGAGCGTCGTGCCGCAACTAGAGAAAGGGCt           >  1:2975560/1‑150 (MQ=255)

                                                                                                                                            tCGCAGTGAAGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAGGGTATTAAAGGCACTGGCGAATGCACGCCGTATCCGCGTTATGGGGAAAGTGATGCCTCGGTAATGGCGCAAATTATGAGCGTCGTGCCGCAACTAGAGAAAGGGCt           >  2:108892/1‑150 (MQ=255)

                                                                                                                                            tCGCAGTGAAGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAGGGTATTAAAGGCACCGGCGAATGCACGCCGTATCCGCGTTATGGGGAAAGTGATGCCTCGGTAATGGCGCAAATTATGAGCGTCGTGCCGCAACTAGAGAAAGGGCt           >  1:1902351/1‑150 (MQ=255)

                                                                                                                                               ccgTGACGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAGGGTATTAAAGGCACCGGCGAATGCACGCCGTATCCGCGGTATGGGGAAAGTGATGCCTCGGTCATGGCGCAAATTATGAGCGTCGTGCCGCACCTAGAGAAAGGGCTGac        >  2:3315840/3‑150 (MQ=255)

                                                                                                                                               cAGTGAAGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAGGGTATTAAAGGCACCGGCGAATGCACGCCGTATCCGCGTTATGGGGAAAGTGATGCCTCGGTAATGGCGCAAATTATGAGCGTCGTGCCGCAACTAGAGAAAGGGCTGac        >  2:520317/1‑150 (MQ=255)

GCTAACTGTCAATAACACAGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAGCGCGCGTGGTGGTGGTTGAACTGGAAGAAGAGGGTATTAAAGGCACCGGCGAATGCACGCCGTATCCGCGTTATGGGGAAAGTGATGCCTCGGTAATGGCGCAAATTATGAGCGTCGTGCCGCAACTAGAGAAAGGGCTGACACGGGA  >  NC_000913/1388858‑1389156

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`