Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 107,554 G→T 11.9% intergenic (+80/‑151) lpxC → / → secM UDP‑3‑O‑acyl N‑acetylglucosamine deacetylase/regulator of secA translation

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913107,5540GT11.9% 596.9 / 9.3 187intergenic (+80/‑151)lpxC/secMUDP‑3‑O‑acyl N‑acetylglucosamine deacetylase/regulator of secA translation
Reads supporting (aligned to +/- strand):  ref base G (17/140);  new base T (26/3);  total (44/143)
Fisher's exact test for biased strand distribution p-value = 2.76e-17
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 192 total aligned reads displayed.

TGTGACCTTCCAGGACGACGCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGT‑GCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGAATATGATCGGGATGGCAATAA  >  NC_000913/107408‑107703
                                                                                                                                                    |                                                                                                                                                      
tgtgACCTTCCAGGACGACGCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGaaa                                                                                                                                                     >  2:4245037/1‑149 (MQ=255)
  tgACCTTCCAGGACGACGCAGAACTGCCGTTGGCCTTCATAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAAttt                                                                                                                                                   <  2:3225179/150‑1 (MQ=255)
   gACCTTCCCGGACGACGCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAAtttt                                                                                                                                                  <  2:3758311/150‑1 (MQ=255)
   gACCTTCCAGGACGACGCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAAtttt                                                                                                                                                  <  1:2547202/150‑1 (MQ=255)
    aCCTTCCAGGACGACGCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGACCCAGTCG‑T‑GTTGTTTTgggtgttt                                                                                                                                                 >  2:751362/1‑143 (MQ=255)
      cTTCCAGGACGACGCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTtata                                                                                                                                               <  1:1947267/150‑1 (MQ=255)
          cAGGACGACGCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTT‑TTTTTTTTGAATTTTATAaga                                                                                                                                            <  2:2589303/150‑1 (MQ=255)
          cAGGACGACGCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAagag                                                                                                                                           <  2:1562309/150‑1 (MQ=255)
          cAGGACGACGCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAagag                                                                                                                                           <  1:3063010/150‑1 (MQ=255)
              acgacgCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCtat                                                                                                                                       <  2:899365/150‑1 (MQ=255)
                gacgCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑GTTTTTTTGTATTTTTTTATAGCTATaa                                                                                                                                     >  1:2330045/1‑150 (MQ=255)
                 acgCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATaaa                                                                                                                                    <  1:3819501/150‑1 (MQ=255)
                 acgCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑GTTTTTTTTTTTTTTATAAGAGCTATaaa                                                                                                                                    >  2:197346/1‑150 (MQ=255)
                      gAACTGCCGTTGGCCTTCAAAGCGCCTTCTGCTGTACTGGAATAACGACATTTATACTGTCTTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCT‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACg                                                                                                                               <  1:2405954/150‑1 (MQ=255)
                               ttGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑GTTTTTTTTTATTTTATTATAGCGATAAAAAAAGGTGGCGAAc                                                                                                                      >  1:690811/1‑150 (MQ=255)
                                 ggCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGT‑GCGAACGCt                                                                                                                   <  1:2338337/150‑1 (MQ=255)
                                 ggCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGT‑GCGAACGCt                                                                                                                   <  1:329352/150‑1 (MQ=255)
                                 ggCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGT‑GCGAACGCt                                                                                                                   <  1:181443/150‑1 (MQ=255)
                                    cTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTTTATTTTTTAATTTATATAAAAAAAGGT‑GAGAAAGCGGtt                                                                                                                >  2:4174939/1‑150 (MQ=255)
                                    cTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGT‑GCGAACGCTGtt                                                                                                                <  1:3561829/150‑1 (MQ=255)
                                     ttCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑GTTTTTTTTTATTTTTTAATTTGTAAAAAAAAACGT‑GCGGACGcgggtt                                                                                                               >  1:2938696/1‑145 (MQ=255)
                                      tCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGT‑GCGAACGCTGtttt                                                                                                              <  2:1725969/150‑1 (MQ=255)
                                        aaaGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGT‑GCGAACGCTGTTTTCt                                                                                                            <  1:2103732/150‑1 (MQ=255)
                                          cgcgcCTTCAGCTGTACTGGCATAACTAAATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCTGTTTAACCTTTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGT‑GCGAACGCTGTTTTCTTa                                                                                                          <  2:2651717/149‑1 (MQ=255)
                                          aGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGT‑GCGAACGCTGTTTTCTTa                                                                                                          <  2:3683750/150‑1 (MQ=255)
                                           gcgcCTTCAGCTGTACTGGCATAACGACATTTATACTGTCTTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGT‑GCGAACGCTGTTTTCTTaa                                                                                                         <  2:257116/150‑1 (MQ=255)
                                           gcgcCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGT‑GCGAACGCTGTTTTCTTaa                                                                                                         <  1:967449/150‑1 (MQ=255)
                                           gcgcCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGT‑GCGAACGCTGTTTTCTTaa                                                                                                         <  1:2450738/150‑1 (MQ=255)
                                           gcgcCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTACAAAATTCGACTGGAAAATCTGGCACTCTCTCCGGCCAGGTGAACCCGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGT‑GCGAACGCTGTTTTCTTaa                                                                                                         <  2:3181075/150‑1 (MQ=255)
                                            cgcCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTT‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGT‑GCGAACGCTGTTTTCTTaa                                                                                                         <  2:4026541/150‑1 (MQ=255)
                                              ccTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑GTTTTTTTTTTTTTTTTAAAAACGATAAAAAAAAAT‑TAGAACGCGGTtttttttataa                                                                                                      >  1:2614100/1‑145 (MQ=255)
                                                    gCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGT‑GCGAACGCTGTTTTCTTAAGCACTTTTc                                                                                                <  2:1136082/150‑1 (MQ=255)
                                                      tGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGT‑GCGAACGCTGTTTTCTTAAGCACTTTTCCg                                                                                              <  1:946540/150‑1 (MQ=255)
                                                      tGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGT‑GCGAACGCTGTTTTCTTAAGCACTTTTCCg                                                                                              <  1:43690/150‑1 (MQ=255)
                                                      tGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGT‑GCGAACGCTGTTTTCTTAAGCACTTTTCCg                                                                                              <  1:2104832/150‑1 (MQ=255)
                                                           tGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGT‑GCGAACGCTGTTTTCTTAAGCACTTTTCCGCACaa                                                                                         <  1:2792501/150‑1 (MQ=255)
                                                            ggCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑GTTTTTTTTTTTTTTATAAAATCGATAAAAAAAAGG‑GCGCACGCGGGTTTGTTAAGCACATTTgcgccaaac                                                                                        >  2:4084945/1‑145 (MQ=255)
                                                            ggCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑GTTTTTTTTTATTTTATAAAAGCGATAAAAAAACGT‑GCGAACCCGGGTTTGTTGAGCACGTTTACGCCCAAc                                                                                        >  2:3069387/1‑150 (MQ=255)
                                                            ggCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑GTTTTTTTGTATTTTTGAAGGGCAATAAAAAACCGG‑GCGCACCCGGGTGTGGTAAGCACATTTACGccccac                                                                                        >  2:1405681/1‑145 (MQ=255)
                                                                 aaCGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGT‑GCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATc                                                                                   <  1:1035549/150‑1 (MQ=255)
                                                                 aaCGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGT‑GCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATc                                                                                   <  1:1035360/150‑1 (MQ=255)
                                                                   cGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTTAATTTTATAAGAGCTATAAAAAAAGGT‑GCGAACGCGTTTTTGTTAATCACTGTTACGCACAACATATAtt                                                                                 >  2:696359/1‑150 (MQ=255)
                                                                     aCATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGT‑GCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCa                                                                               <  2:3922827/150‑1 (MQ=255)
                                                                           ataCTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGT‑GCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTg                                                                         <  1:4017099/150‑1 (MQ=255)
                                                                           ataCTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGT‑GCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTg                                                                         <  1:3511199/150‑1 (MQ=255)
                                                                            taCTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑GTTTTTTTTTTTTTTATAATAGCTATAAAAAAAGGT‑GCGGGCGCGGTTTTGTTTATCACATTTACGCACAAATTATGGTAATTCGTgg                                                                        >  2:565631/1‑149 (MQ=255)
                                                                                gTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGT‑GCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCtgtg                                                                    <  1:3385199/150‑1 (MQ=255)
                                                                                     ataAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGT‑GCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTg                                                               <  2:3399050/150‑1 (MQ=255)
                                                                                       aaacTTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGT‑GCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCa                                                             <  2:2678148/150‑1 (MQ=255)
                                                                                       aaaaTTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGT‑GCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCa                                                             <  2:578054/150‑1 (MQ=255)
                                                                                       aaaaTTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGT‑GCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCa                                                             <  2:817191/150‑1 (MQ=255)
                                                                                         aaTTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑A‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGT‑GCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAgg                                                           <  2:734661/150‑1 (MQ=255)
                                                                                              gACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTTTATTTTTTAAGAGCTATAAAAAACGGT‑GCGCACGCGGTTTTGTTAAGCACTTTTACGCACAACATATATTAAATAGTGCTGGGGGCTGCAGGCTTTa                                                      >  2:2809323/1‑150 (MQ=255)
                                                                                               aCTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGT‑GCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTaa                                                     <  2:115134/150‑1 (MQ=255)
                                                                                               aCTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGT‑GCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTaa                                                     <  1:3904686/150‑1 (MQ=255)
                                                                                               aCTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGT‑GCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTaa                                                     <  1:2047569/150‑1 (MQ=255)
                                                                                                    cAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGT‑GCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATa                                                <  2:1869841/150‑1 (MQ=255)
                                                                                                     aaaTCTGGCACTCTCTCCGGCCAGGTGAAACAGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGT‑GCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATaa                                               <  2:1822839/150‑1 (MQ=255)
                                                                                                        tCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑GTTTTTTTTAATTTTTAAATATGTATAAAAAAAAGT‑GGGAACACGGTTTTATTTAGCACATTTACGCACAACAAATATTAAATCTGGGTGTGGTCAGGAGGCGTTAATTATAAGaa                                            >  2:3598932/1‑149 (MQ=255)
                                                                                                          tGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGT‑GCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGAttt                                          <  2:1276918/150‑1 (MQ=255)
                                                                                                            gCACTCTCTCCGGCCAGGTGAACCTGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGT‑GCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTtgt                                        <  1:836611/150‑1 (MQ=255)
                                                                                                            gCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGT‑GCGAACGCTGTTTTCTTAAGCTCTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTtgt                                        <  2:3272436/150‑1 (MQ=255)
                                                                                                            gCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGT‑GCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTtgt                                        <  2:641649/150‑1 (MQ=255)
                                                                                                            gCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGT‑GCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTtgt                                        <  1:1919122/150‑1 (MQ=255)
                                                                                                              aCTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAATATCTAAAAAAAAAGGT‑GGGAAAGCTGGTTTAGTAAGCACATTTACGCACAACATATAATAATTCGTGGGGTGGAAATCAGGCTTTAATTATAATATTTTTgc                                      >  2:2505275/1‑150 (MQ=255)
                                                                                                                tctctcCGGCCAGGTGAACCAGTCGTT‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGT‑GCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTgcg                                     <  2:3502676/150‑1 (MQ=255)
                                                                                                                tctctcCGGCCAGGTGAACCAGTCGTT‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGT‑GCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTgcg                                     <  2:2919651/150‑1 (MQ=255)
                                                                                                                tctctcCGGCCAGGTGAACCAGTCG‑TGTTTTTTTTTAATTTT‑TAATATCGATAAAAAACCGT‑GGGGACGCGGTTTTGTTAAGCACATTTACGCACCACATAAATTAAATAGTTCTGTGGGCGGCCGGCTGTAATTTTAAGATTTTTgggc                                    >  2:3784973/1‑150 (MQ=255)
                                                                                                                tctctcCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGT‑GCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTgcgc                                    <  2:2197669/150‑1 (MQ=255)
                                                                                                                   ctcCGGCCAGGTGAACCAGTCG‑T‑GTTTTTTTTTAATTTATAAAATATAAAAAAAAAGGT‑GGGCAACCGGTTTTGTTTAGCACTGTTGGGCACAACATATCTTCATTCTTGGTGGGGGGTGCAGGCGTTAATGATAATAATtgtgggggga                                 >  2:2243478/1‑144 (MQ=255)
                                                                                                                    tcCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGT‑GCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTaaa                                <  1:41217/150‑1 (MQ=255)
                                                                                                                        gCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGT‑GCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACg                            <  2:2977513/150‑1 (MQ=255)
                                                                                                                        gCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGT‑GCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACg                            <  2:774218/150‑1 (MQ=255)
                                                                                                                        gCCAGGTGAACCAGTCG‑T‑TTTTTTTT‑AATTTTATAATAGCTAAAAAAAAAGGT‑TCGAACGCTGTTTTGTTAATCACATTTAAGCACAACATATCGTCATTAGTGCTGGGGTCGGCAGGCTTTAATGATAATAATTTTGCGCAAAATAAGt                           >  2:1761296/1‑150 (MQ=255)
                                                                                                                          cAGGTGAACCCGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGT‑GCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGtt                          <  1:751362/150‑1 (MQ=255)
                                                                                                                          cAGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGT‑GCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGtt                          <  2:2998198/150‑1 (MQ=255)
                                                                                                                          cAGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGT‑GCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGtt                          <  1:2626854/150‑1 (MQ=255)
                                                                                                                           aGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGT‑GCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGttt                         <  2:2725645/150‑1 (MQ=255)
                                                                                                                              tGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGT‑GCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGaa                      <  1:2960042/150‑1 (MQ=255)
                                                                                                                              tGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGT‑GCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGaa                      <  2:1104680/150‑1 (MQ=255)
                                                                                                                                  ccAGTCT‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGT‑GCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGAATATg                  <  1:2702882/150‑1 (MQ=255)
                                                                                                                                     gTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGT‑GCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGAATATGATc               <  1:3150789/150‑1 (MQ=255)
                                                                                                                                     gTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGT‑GCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGAATATGATc               <  1:2163172/150‑1 (MQ=255)
                                                                                                                                      tCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGT‑GCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGAATATGATCg              >  2:3422599/1‑150 (MQ=255)
                                                                                                                                        g‑T‑TTTTTTTTTAATTTTATAAGAGCTATAAAAAACGGT‑GCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGAATATGATCggg            >  1:1150916/1‑150 (MQ=255)
                                                                                                                                        g‑T‑TTTTTTTTTAATTTTATAAGAGCTATAAAAAACGGT‑GCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTATAATGATAAGATTTGTGCGCTAAATACGTTTGAATATGATCggg            >  1:3956224/1‑150 (MQ=255)
                                                                                                                                        g‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGT‑GCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGGGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGAATATGATAggg            >  1:1176257/1‑150 (MQ=255)
                                                                                                                                          t‑ttttttttGAATTTTATAAGAGCTATAAAAAACGGT‑GCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGAATATGATCGGGa           <  1:4084945/150‑1 (MQ=255)
                                                                                                                                          t‑ttttttttGAATTTTATAAGAGCTATAAAAAACGGT‑GCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGAATATGATCGGGa           <  1:3069387/150‑1 (MQ=255)
                                                                                                                                          t‑ttttttttGAATTTTATAAGAGCTATAAAAAACGGT‑GCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGAATATGATCGGGa           <  1:1405681/150‑1 (MQ=255)
                                                                                                                                           attttttttGAATTTTATAAGAGCTATAAAAAACGGT‑GCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGAATATGATCGGGa           <  2:3840129/149‑1 (MQ=255)
                                                                                                                                             tttttttGAATTTTATAAGAGCTATAAAAAACGGT‑GCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGAATATGATCGGGATg         >  2:2146846/1‑150 (MQ=255)
                                                                                                                                             tttttttGAATTTTATAAGAGCTATAAAAAACGGT‑GCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGAATATGATCGGGATg         <  1:3077772/150‑1 (MQ=255)
                                                                                                                                              ttttttGAATTTTATAAGAGCTATAAAAAACGGT‑GCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGAATATGATCGGGATgg        <  1:3703941/150‑1 (MQ=255)
                                                                                                                                               tttttGAATTTTATAAGAGCTATAAAAAACGGT‑GCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGAATATGATCGGGATGGc       <  1:469755/150‑1 (MQ=255)
                                                                                                                                               tttttGAATTTTATAAGAGCTATAAAAAACGGT‑GCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGAATATGATCGGGATGGc       <  1:2809323/150‑1 (MQ=255)
                                                                                                                                               tttttGAATTTTATAAGAGCTATAAAAAACGGT‑GCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTACCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGAATATGATCGGGATGGc       >  2:1326248/1‑150 (MQ=255)
                                                                                                                                                ttgtGAATTTTATAAGAGCTATAAAAAACGGT‑GCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTCTCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAGGATTTGTGCGCTAAATACGTGTGAAGAGGATCGGGATGGCa      >  2:2024626/4‑150 (MQ=255)
                                                                                                                                                    gAATTTTATAAGAGCTATAAAAAACGGT‑GCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGAATATGATCGGGATGGCAATaa  >  2:4050807/1‑150 (MQ=255)
                                                                                                                                                    gAATTTTATAAGAGCTATAAAAAACGGT‑GCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGAATATGATCGGGATGGCAATaa  <  2:1032890/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                      
TGTGACCTTCCAGGACGACGCAGAACTGCCGTTGGCCTTCAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCG‑T‑TTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGT‑GCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGACTGCAGGCTTTAATGATAAGATTTGTGCGCTAAATACGTTTGAATATGATCGGGATGGCAATAA  >  NC_000913/107408‑107703

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑