Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,543,230 C→G 7.5% M277I (ATG→ATC) ‡ narU ← nitrate/nitrite transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,543,2300CG 7.5% 849.1 / 48.9 238M277I (ATG→ATC) ‡narUnitrate/nitrite transporter
Reads supporting (aligned to +/- strand):  ref base C (111/109);  new base G (10/8);  total (121/117)
Fisher's exact test for biased strand distribution p-value = 8.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

Only 100 of 238 total aligned reads displayed.

CCATAAAAATAAAGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATCCCTGACCATG  >  NC_000913/1543082‑1543378
                                                                                                                                                    |                                                                                                                                                     
ccATAAAAATAAAGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCa                                                                                                                                                      <  1:992697/150‑1 (MQ=255)
  aTAAAAATAAAGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGACCTGGGTTTTTGCCAGCATg                                                                                                                                                    >  2:561822/1‑150 (MQ=255)
   tAAAAATAAAGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATgg                                                                                                                                                   <  2:1918564/150‑1 (MQ=255)
   tAAAAATAAAGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATgg                                                                                                                                                   >  2:2200868/1‑150 (MQ=255)
   tAAAAATAAAGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATgg                                                                                                                                                   >  2:2311593/1‑150 (MQ=255)
   tAAAAATAAAGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATgg                                                                                                                                                   <  2:680907/150‑1 (MQ=255)
        aTAAAGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑aaaa                                                                                                                                             >  2:1479349/1‑150 (MQ=255)
          aaaGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGG‑TTTTGCCATGCTGGC‑AAAAacc                                                                                                                                          <  2:1403309/150‑1 (MQ=255)
            agctGATCAACGTCACCCGCACGCCGCCGAACGTCTCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACCTCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCg                                                                                                                                         <  2:1162909/147‑1 (MQ=255)
              ttGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCa                                                                                                                                       <  2:3890877/150‑1 (MQ=255)
                 aTCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGaa                                                                                                                                    >  2:421276/1‑150 (MQ=255)
                     aCGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAAc                                                                                                                                <  1:3553376/150‑1 (MQ=255)
                     aCGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAAc                                                                                                                                >  2:2100312/1‑150 (MQ=255)
                        tCACCCGCCCGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAACGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGa                                                                                                                             <  1:1822757/150‑1 (MQ=255)
                            ccGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCCGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATaaa                                                                                                                         >  1:511413/1‑150 (MQ=255)
                            ccGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATaaa                                                                                                                         >  1:758196/1‑150 (MQ=255)
                            ccGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATaaa                                                                                                                         >  1:511236/1‑150 (MQ=255)
                            ccGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATaaa                                                                                                                         <  1:3315324/150‑1 (MQ=255)
                             cGCACGCCGCCGAACTTATCGGAAATAGCACCACCAAACGAACGCGCGATTGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑ACAACCCGAAGAACAAACGATAAAc                                                                                                                        >  2:2709308/1‑150 (MQ=255)
                               cACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGTCGCATATTATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGa                                                                                                                      <  1:203086/150‑1 (MQ=255)
                               cACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGa                                                                                                                      <  1:2408463/150‑1 (MQ=255)
                               cACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGa                                                                                                                      <  1:2319685/150‑1 (MQ=255)
                                aCGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGaa                                                                                                                     <  1:3711216/150‑1 (MQ=255)
                                      ccgAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAg                                                                                                               >  2:3381978/1‑150 (MQ=255)
                                      ccgAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAg                                                                                                               >  2:1328519/1‑150 (MQ=255)
                                       cgAACTTATCGTAAATAGCACCACCAACCGACCGCGCGTTGGCACCTTTAATTGGGCCAAATAACGCCTGGCGCAGAATATTCACATCGGGTAATTGGGTTTTTGCCAGCATGGC‑AAAACCCGAAGAAAAACCTATAAACGAACCGAAgg                                                                                                              <  1:2950413/150‑1 (MQ=255)
                                        gAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGt                                                                                                             >  2:2112807/1‑150 (MQ=255)
                                        gAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGt                                                                                                             >  2:3799008/1‑150 (MQ=255)
                                          aCTTATCGGAAATAGCACCACCACCCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGTAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTgg                                                                                                           <  1:4146134/150‑1 (MQ=255)
                                            ttATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCa                                                                                                         >  1:1867134/1‑150 (MQ=255)
                                             tATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCaa                                                                                                        <  2:2980942/150‑1 (MQ=255)
                                             tATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCaa                                                                                                        <  2:843340/150‑1 (MQ=255)
                                                 ggAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTa                                                                                                    >  1:2305048/1‑150 (MQ=255)
                                                 ggAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTa                                                                                                    >  1:2624475/1‑150 (MQ=255)
                                                  gAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTaa                                                                                                   >  1:4238236/1‑150 (MQ=255)
                                                  gAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTaa                                                                                                   >  1:254524/1‑150 (MQ=255)
                                                   caagaGCACCACCCACCGACCGCGCGAGGCCACCGCTAACTGGGCCACCGCACGCCAGGCGCAGAATCTTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAACCCGATAAACGAACCGAAGGTGGCAAGGTaaa                                                                                                  <  2:2804834/146‑1 (MQ=255)
                                                   aaaTAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTaaa                                                                                                  <  1:2425303/150‑1 (MQ=255)
                                                   aaaTAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTaaa                                                                                                  >  1:609687/1‑150 (MQ=255)
                                                   aaaTAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTaaa                                                                                                  <  1:3059628/150‑1 (MQ=255)
                                                      tAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGACCTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGGGGCACGGTAAAGcc                                                                                               >  1:1402496/1‑149 (MQ=255)
                                                           ccaccaACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTc                                                                                          <  2:3519486/150‑1 (MQ=255)
                                                            caccaACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTca                                                                                         >  2:1278593/1‑150 (MQ=255)
                                                               caACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTcagca                                                                                      <  1:2816232/150‑1 (MQ=255)
                                                                 accgaccgCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCagcagc                                                                                    <  2:1659300/150‑1 (MQ=255)
                                                                       cgcgcgATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAgaga                                                                              >  2:4162362/1‑150 (MQ=255)
                                                                       cgcgcgATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAgaga                                                                              >  1:3600159/1‑150 (MQ=255)
                                                                         cgcgATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATg                                                                            <  2:1945890/150‑1 (MQ=255)
                                                                            gATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAg                                                                         <  1:4166078/150‑1 (MQ=255)
                                                                               ggCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCg                                                                      <  1:3206360/150‑1 (MQ=255)
                                                                                gCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGt                                                                     <  2:3351150/150‑1 (MQ=255)
                                                                                gCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGt                                                                     <  1:1021348/150‑1 (MQ=255)
                                                                                 cACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGtt                                                                    <  2:3270585/150‑1 (MQ=255)
                                                                                  aCCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTg                                                                   >  2:571673/1‑150 (MQ=255)
                                                                                       tAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGa                                                              <  2:746215/150‑1 (MQ=255)
                                                                                       tAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGa                                                              <  1:3791398/150‑1 (MQ=255)
                                                                                         aaTGGGCCAAAGTACGCCAGGCGCAGACTCTTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACa                                                            <  2:2376352/150‑1 (MQ=255)
                                                                                         aaTGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACa                                                            >  1:1422198/1‑150 (MQ=255)
                                                                                           tGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAgg                                                          <  1:1768188/150‑1 (MQ=255)
                                                                                           tGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAgg                                                          <  1:1189973/150‑1 (MQ=255)
                                                                                           tGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGATGTAAGACAgg                                                          >  1:2547476/1‑150 (MQ=255)
                                                                                             ggCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGa                                                        >  2:1232179/1‑150 (MQ=255)
                                                                                             ggCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGa                                                        >  2:1951109/1‑150 (MQ=255)
                                                                                             ggCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGa                                                        >  2:2115421/1‑150 (MQ=255)
                                                                                              gCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAg                                                       >  1:1129611/1‑150 (MQ=255)
                                                                                               ccAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCCGCAATGC‑AAAACCAGCAGAAAAACCGATAAACGAACCGAAGGTGGCAACGTAAAGCAGTCTCAGCATCCAGAGATGCAGGCGTTGTAAGACAGGGAGc                                                      >  1:2588971/1‑150 (MQ=255)
                                                                                                cAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCt                                                     >  1:2838612/1‑150 (MQ=255)
                                                                                                cAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGCC‑AAAACCCGCCGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAGGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGcg                                                     >  2:2609946/1‑149 (MQ=255)
                                                                                                  aaGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTgg                                                   >  1:2498126/1‑150 (MQ=255)
                                                                                                         ccAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAGCCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCaa                                            >  2:974860/1‑150 (MQ=255)
                                                                                                           aGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAAtt                                          <  1:3116015/150‑1 (MQ=255)
                                                                                                             gcgcAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGa                                        >  2:1457894/1‑150 (MQ=255)
                                                                                                              cgcAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAg                                       <  1:4200168/150‑1 (MQ=255)
                                                                                                                cAGAATATTCACATCCGGGAACTGGGTTATTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGgc                                     <  2:442667/150‑1 (MQ=255)
                                                                                                                    atatTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGt                                 <  1:1328519/150‑1 (MQ=255)
                                                                                                                       ttCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGaa                              <  2:1552424/150‑1 (MQ=255)
                                                                                                                        tCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAAc                             >  1:2720390/1‑150 (MQ=255)
                                                                                                                        tCACATCCCGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAAc                             <  1:4116491/150‑1 (MQ=255)
                                                                                                                         cacaTCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACt                            <  1:901806/150‑1 (MQ=255)
                                                                                                                           caTCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTgg                          <  1:733652/150‑1 (MQ=255)
                                                                                                                            aTCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGc                         >  2:1414225/1‑150 (MQ=255)
                                                                                                                            aTCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGc                         <  1:2113995/150‑1 (MQ=255)
                                                                                                                            aTCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGc                         <  1:406243/150‑1 (MQ=255)
                                                                                                                              ccGGGTACTGGTTTTTTGCCAGCATGTC‑AAAACCCGCAGAAAAACCGATATACGAACCGAATGTTGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGa                       <  1:1593287/150‑1 (MQ=255)
                                                                                                                              ccGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGa                       >  2:3974290/1‑150 (MQ=255)
                                                                                                                                gggAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCCGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGata                     >  1:1719543/1‑150 (MQ=255)
                                                                                                                                 ggAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGatat                    <  2:3219531/150‑1 (MQ=255)
                                                                                                                                  gAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCATGAACTGGCGATAtc                   <  1:217419/150‑1 (MQ=255)
                                                                                                                                   aaCTGGGTTTTTGCCAGCATGGC‑AAATCCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCATGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATAtca                  <  1:3364943/150‑1 (MQ=255)
                                                                                                                                     tgcgggttttGCCATGCTGGCAAAAACCCGCAGAAAAACCGAGAAACGAACCGAAGGTGGCAAGGTCAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTCCGACCGGGAGCTGGTCGGCCCTTGAGGCGCGTGAACTGGCGATAtcat                 >  2:943749/7‑150 (MQ=255)
                                                                                                                                      tGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAGGGTGGCAAGGTAAAGCAGGCTCAGCTGCCAGAGATGCAGGCGTTGTTGGACGGGGAGCTGGTCGGCACTTGTGGCGCGTGAGCTGGCGATAtccttc               >  2:509978/1‑150 (MQ=255)
                                                                                                                                      tGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGAGCGTGAACTGGCGATAtcattc               >  2:2557655/1‑150 (MQ=255)
                                                                                                                                      tGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGAGAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAAGTGAGGCGCGTGAACTGGCGATAtcattc               >  2:4132646/1‑150 (MQ=255)
                                                                                                                                       cgggttttGCCATGCTGGCAAAAAACCGCAGAAAAACCGATAAACGAACCCAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGAAAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATAtcattc               >  1:11766/5‑150 (MQ=255)
                                                                                                                                       cgggttttGCCATGCTGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATAtcattca              >  2:1798739/5‑150 (MQ=255)
                                                                                                                                        gggttttGCCATGCTGGC‑AAAAACCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATAtcattcat             >  1:1934566/4‑150 (MQ=255)
                                                                                                                                           ttttGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATccc          >  1:1840101/1‑150 (MQ=255)
                                                                                                                                           ttttGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATccc          >  1:4099163/1‑150 (MQ=255)
                                                                                                                                            tttGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATCCCt         <  1:773383/150‑1 (MQ=255)
                                                                                                                                              tGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAAGGAACCGCAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGGGTGAACTGGCGATATCATTCATCCCTGa       <  1:3956442/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CCATAAAAATAAAGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGC‑AAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATCCCTGACCATG  >  NC_000913/1543082‑1543378

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑