Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,543,231 A→C 7.5% M277R (ATG→AGG) ‡ narU ← nitrate/nitrite transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,543,2310AC 7.5% 792.3 / 38.5 237M277R (ATG→AGG) ‡narUnitrate/nitrite transporter
Reads supporting (aligned to +/- strand):  ref base A (111/107);  new base C (10/8);  total (121/116)
Fisher's exact test for biased strand distribution p-value = 8.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 239 total aligned reads displayed.

CCATAAAAATAAAGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATCCCTGACCATGCG  >  NC_000913/1543082‑1543380
                                                                                                                                                     |                                                                                                                                                      
ccATAAAAATAAAGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCa                                                                                                                                                        <  1:992697/150‑1 (MQ=255)
  aTAAAAATAAAGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGACCTGGGTTTTTGCCAGCATg                                                                                                                                                      >  2:561822/1‑150 (MQ=255)
   tAAAAATAAAGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATgg                                                                                                                                                     >  2:2200868/1‑150 (MQ=255)
   tAAAAATAAAGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATgg                                                                                                                                                     <  2:680907/150‑1 (MQ=255)
   tAAAAATAAAGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATgg                                                                                                                                                     >  2:2311593/1‑150 (MQ=255)
        aTAAAGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCaaaa                                                                                                                                               >  2:1479349/1‑150 (MQ=255)
          aaaGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTGCCATGCTGGCAAAAacc                                                                                                                                            <  2:1403309/150‑1 (MQ=255)
            agctGATCAACGTCACCCGCACGCCGCCGAACGTCTCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACCTCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCg                                                                                                                                           <  2:1162909/147‑1 (MQ=255)
              ttGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCa                                                                                                                                         <  2:3890877/150‑1 (MQ=255)
                 aTCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGaa                                                                                                                                      >  2:421276/1‑150 (MQ=255)
                     aCGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAAc                                                                                                                                  <  1:3553376/150‑1 (MQ=255)
                     aCGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAAc                                                                                                                                  <  1:1954752/150‑1 (MQ=255)
                        tCACCCGCCCGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAACGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGa                                                                                                                               <  1:1822757/150‑1 (MQ=255)
                          aCCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATa                                                                                                                             >  2:1185743/1‑150 (MQ=255)
                            ccGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATaaa                                                                                                                           <  1:3315324/150‑1 (MQ=255)
                            ccGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATaaa                                                                                                                           >  1:3985508/1‑150 (MQ=255)
                            ccGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATaaa                                                                                                                           >  1:511236/1‑150 (MQ=255)
                            ccGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATaaa                                                                                                                           >  1:511410/1‑150 (MQ=255)
                             cGCACGCCGCCGAACTTATCGGAAATAGCACCACCAAACGAACGCGCGATTGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCACAACCCGAAGAACAAACGATAAAc                                                                                                                          >  2:2709308/1‑150 (MQ=255)
                               cACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGTCGCATATTATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGa                                                                                                                        <  1:203086/150‑1 (MQ=255)
                               cACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGa                                                                                                                        <  1:2408463/150‑1 (MQ=255)
                                aCGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGaa                                                                                                                       <  1:883374/150‑1 (MQ=255)
                                aCGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGaa                                                                                                                       <  1:3711216/150‑1 (MQ=255)
                                      ccgAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAg                                                                                                                 >  2:3381978/1‑150 (MQ=255)
                                       cgAACTTATCGTAAATAGCACCACCAACCGACCGCGCGTTGGCACCTTTAATTGGGCCAAATAACGCCTGGCGCAGAATATTCACATCGGGTAATTGGGTTTTTGCCAGCATGGCAAAACCCGAAGAAAAACCTATAAACGAACCGAAgg                                                                                                                <  1:2950413/150‑1 (MQ=255)
                                        gAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGt                                                                                                               >  2:2112807/1‑150 (MQ=255)
                                        gAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGt                                                                                                               >  2:3799008/1‑150 (MQ=255)
                                            ttATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCa                                                                                                           >  1:1867134/1‑150 (MQ=255)
                                             tATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCaa                                                                                                          <  2:843340/150‑1 (MQ=255)
                                                 ggAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTa                                                                                                      >  1:2624475/1‑150 (MQ=255)
                                                 ggAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTa                                                                                                      >  1:2305048/1‑150 (MQ=255)
                                                  gAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTaa                                                                                                     >  2:2974770/1‑150 (MQ=255)
                                                  gAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTaa                                                                                                     >  1:254524/1‑150 (MQ=255)
                                                  gAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTaa                                                                                                     <  2:13214/150‑1 (MQ=255)
                                                   caagaGCACCACCCACCGACCGCGCGAGGCCACCGCTAACTGGGCCACCGCACGCCAGGCGCAGAATCTTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAACCCGATAAACGAACCGAAGGTGGCAAGGTaaa                                                                                                    <  2:2804834/146‑1 (MQ=255)
                                                   aaaTAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTaaa                                                                                                    <  1:3059628/150‑1 (MQ=255)
                                                   aaaTAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTaaa                                                                                                    >  1:609687/1‑150 (MQ=255)
                                                   aaaTAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTaaa                                                                                                    <  1:2425303/150‑1 (MQ=255)
                                                      tAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGACCTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGGGGCACGGTAAAGcc                                                                                                 >  1:1402496/1‑149 (MQ=255)
                                                           ccaccaACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTc                                                                                            <  2:3519486/150‑1 (MQ=255)
                                                            caccaACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTca                                                                                           >  2:1278593/1‑150 (MQ=255)
                                                            caccaACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTca                                                                                           >  1:4141518/1‑150 (MQ=255)
                                                               caACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTcagca                                                                                        <  1:2816232/150‑1 (MQ=255)
                                                                 accgaccgCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAATACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCagcagc                                                                                      <  2:1659166/150‑1 (MQ=255)
                                                                     accgCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCaga                                                                                  <  1:3381978/150‑1 (MQ=255)
                                                                       cgcgcgATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAgaga                                                                                >  2:4162362/1‑150 (MQ=255)
                                                                       cgcgcgATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAgaga                                                                                >  1:3600357/1‑150 (MQ=255)
                                                                        gcgcgATGGCCCCGATACATGGGCCAAAGAACGCCCGGCGCAGAAAATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCCAAACCCGCAGAAAAACCGATCAACGAACCGAAGGTGGCACGGTACAGCAGGCTCAGCAGCCCGAGAt                                                                               >  2:3956442/1‑150 (MQ=255)
                                                                         cgcgATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATg                                                                              <  2:1945890/150‑1 (MQ=255)
                                                                            gATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAg                                                                           <  1:4166078/150‑1 (MQ=255)
                                                                            gATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAg                                                                           <  2:2346426/150‑1 (MQ=255)
                                                                               ggCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCg                                                                        <  1:3206360/150‑1 (MQ=255)
                                                                                gCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGt                                                                       <  1:1021348/150‑1 (MQ=255)
                                                                                 cACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGtt                                                                      <  2:3270585/150‑1 (MQ=255)
                                                                                  aCCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTg                                                                     >  2:571673/1‑150 (MQ=255)
                                                                                       tAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGa                                                                <  1:3791398/150‑1 (MQ=255)
                                                                                       tAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGa                                                                <  2:746215/150‑1 (MQ=255)
                                                                                         aaTGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACa                                                              <  1:4212064/150‑1 (MQ=255)
                                                                                         aaTGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACa                                                              <  2:1854910/150‑1 (MQ=255)
                                                                                         aaTGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACa                                                              >  1:1422198/1‑150 (MQ=255)
                                                                                           tGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAgg                                                            <  1:2320785/150‑1 (MQ=255)
                                                                                           tGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAgg                                                            <  1:1768188/150‑1 (MQ=255)
                                                                                           tGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAgg                                                            <  1:1189973/150‑1 (MQ=255)
                                                                                           tGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGATGTAAGACAgg                                                            >  1:2547476/1‑150 (MQ=255)
                                                                                             ggCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGa                                                          >  2:1951109/1‑150 (MQ=255)
                                                                                             ggCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGa                                                          >  2:2115421/1‑150 (MQ=255)
                                                                                              gCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAg                                                         >  1:1129611/1‑150 (MQ=255)
                                                                                               ccAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCCGCAATGCAAAACCAGCAGAAAAACCGATAAACGAACCGAAGGTGGCAACGTAAAGCAGTCTCAGCATCCAGAGATGCAGGCGTTGTAAGACAGGGAGc                                                        >  1:2588971/1‑150 (MQ=255)
                                                                                               ccAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGc                                                        >  1:643037/1‑150 (MQ=255)
                                                                                                cAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCt                                                       >  1:2838612/1‑150 (MQ=255)
                                                                                                cAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGCCAAAACCCGCCGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAGGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGcg                                                       >  2:2609946/1‑149 (MQ=255)
                                                                                                         ccAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAGCCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCaa                                              >  2:974860/1‑150 (MQ=255)
                                                                                                             gcgcAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGa                                          <  1:3139795/150‑1 (MQ=255)
                                                                                                             gcgcAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGa                                          <  1:251311/150‑1 (MQ=255)
                                                                                                              cgcAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAg                                         <  1:4199979/150‑1 (MQ=255)
                                                                                                                cAGAATATTCACATCCGGGAACTGGGTTATTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGgc                                       <  2:442667/150‑1 (MQ=255)
                                                                                                                    atatTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGt                                   <  1:1328519/150‑1 (MQ=255)
                                                                                                                       ttCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGaa                                <  2:1552424/150‑1 (MQ=255)
                                                                                                                       ttCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGaa                                >  2:1452918/1‑150 (MQ=255)
                                                                                                                        tCACATCCCGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAAc                               <  1:4116491/150‑1 (MQ=255)
                                                                                                                            aTCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGc                           <  1:2113995/150‑1 (MQ=255)
                                                                                                                            aTCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGc                           <  1:406279/150‑1 (MQ=255)
                                                                                                                            aTCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGc                           >  2:1414225/1‑150 (MQ=255)
                                                                                                                              ccGGGTACTGGTTTTTTGCCAGCATGTCAAAACCCGCAGAAAAACCGATATACGAACCGAATGTTGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGa                         <  1:1593287/150‑1 (MQ=255)
                                                                                                                                gggAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCCGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGata                       >  1:1719543/1‑150 (MQ=255)
                                                                                                                                 ggAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGatat                      <  2:3219531/150‑1 (MQ=255)
                                                                                                                                  gAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCATGAACTGGCGATAtc                     <  1:217419/150‑1 (MQ=255)
                                                                                                                                     tgcgggttttGCCATGCTGGCAAAAACCCGCAGAAAAACCGAGAAACGAACCGAAGGTGGCAAGGTCAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTCCGACCGGGAGCTGGTCGGCCCTTGAGGCGCGTGAACTGGCGATAtcat                   >  2:943749/7‑150 (MQ=255)
                                                                                                                                      tGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAGGGTGGCAAGGTAAAGCAGGCTCAGCTGCCAGAGATGCAGGCGTTGTTGGACGGGGAGCTGGTCGGCACTTGTGGCGCGTGAGCTGGCGATAtccttc                 >  2:509978/1‑150 (MQ=255)
                                                                                                                                      tGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATAtcattc                 >  1:748384/1‑150 (MQ=255)
                                                                                                                                      tGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGAGCGTGAACTGGCGATAtcattc                 >  2:2557655/1‑150 (MQ=255)
                                                                                                                                      tGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGAGAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAAGTGAGGCGCGTGAACTGGCGATAtcattc                 >  2:4132646/1‑150 (MQ=255)
                                                                                                                                       cgggttttGCCATGCTGGCAAAAAACCGCAGAAAAACCGATAAACGAACCCAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGAAAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATAtcattc                 >  1:11766/5‑150 (MQ=255)
                                                                                                                                       cgggttttGCCATGCTGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATAtcattca                >  2:1798739/5‑150 (MQ=255)
                                                                                                                                        ggTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATAtcattcat               >  1:2784591/1‑150 (MQ=255)
                                                                                                                                         gTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGACCTGGCGATATCATTCATc              >  2:203313/1‑150 (MQ=255)
                                                                                                                                         gTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGAGATATCATTCATc              <  2:3337958/150‑1 (MQ=255)
                                                                                                                                           ttttGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATccc            >  1:1840101/1‑150 (MQ=255)
                                                                                                                                            tttGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATCCCt           <  1:773383/150‑1 (MQ=255)
                                                                                                                                              tGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAAGGAACCGCAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGGGTGAACTGGCGATATCATTCATCCCTGa         <  1:3956442/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
CCATAAAAATAAAGTTGATCAACGTCACCCGCACGCCGCCGAACTTATCGGAAATAGCACCACCAACCGACCGCGCGATGGCACCGATAAATGGGCCAAAGAACGCCAGGCGCAGAATATTCACATCCGGGAACTGGGTTTTTGCCAGCATGGCAAAACCCGCAGAAAAACCGATAAACGAACCGAAGGTGGCAAGGTAAAGCAGGCTCAGCAGCCAGAGATGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATCCCTGACCATGCG  >  NC_000913/1543082‑1543380

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: