Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,642,373 C→G 5.5% intergenic (+306/+116) cspF → / ← quuQ Qin prophage; cold shock protein/Qin prophage; putative antitermination protein Q

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,642,3730CG 5.5% 827.6 / 27.9 229intergenic (+306/+116)cspF/quuQQin prophage; cold shock protein/Qin prophage; putative antitermination protein Q
Reads supporting (aligned to +/- strand):  ref base C (131/83);  new base G (11/2);  total (144/85)
Fisher's exact test for biased strand distribution p-value = 1.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 240 total aligned reads displayed.

TTGTGTTTAGGGACATTGTACTGGAAGAAAACATTTTAAACATCAGGCAAATAACCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCATCTTCAGCAAAAATCATTTATCTCGTCACCTTCCTCAATTGCGCTTCCG  >  NC_000913/1642224‑1642520
                                                                                                                                                     |                                                                                                                                                   
  gtgtTTAGGGACATTGTACTGGAAGAAAACATTTTAAACATCAGGCAAATAACCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAg                                                                                                                                                   >  2:4054225/1‑150 (MQ=255)
   tgtTTAGGGACATTGTACTGGAAGAAAACATTTTAAACATCAGGCAAATAACCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGc                                                                                                                                                  <  1:540999/150‑1 (MQ=255)
       tAGGGACATTGTACTGGAAGAAAACATTTTAAACATCAGGCAAATAACCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGt                                                                                                                                              >  1:1481111/1‑150 (MQ=255)
       tAGGGACATTGTACTGGAAGAAAACATTTTAAACATCAGGCAAATAACCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGt                                                                                                                                              >  1:1713819/1‑150 (MQ=255)
       tAGGGACATTGTACTGGAAGAAAACATTTTAAACATCAGGCAAATAACCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGt                                                                                                                                              >  1:2018813/1‑150 (MQ=255)
        aGGGACATTGTACTGGAAGAAAACATTTTAAACATCAGGCAAATAACCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGtt                                                                                                                                             >  1:687885/1‑150 (MQ=255)
          ggACATTGTACTGGAAGAAAACATTTTAAACATCAGGCAAATAACCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGtttt                                                                                                                                           >  2:1466960/1‑150 (MQ=255)
            accttGTACTGGAAGAAAACATTTTAAACATCCGGCAAATACCCAAGTCACCAGCTCAATAATCCGGTACCAGACATGAGTCCCGGGATGAGAGTCAACATTACCAGTGCCCCATTTAAAGCACCACACCCGCTGATGAGCGGGttttgt                                                                                                                                         <  2:895223/147‑3 (MQ=255)
             cATTGTACTGGAAGAAAACATTTTAAACATCAGGCAAATAACCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGGGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTa                                                                                                                                        <  1:624499/150‑1 (MQ=255)
             cATTGTACTGGAAGAAAACATTTTAAACATCAGGCAAATAACCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTa                                                                                                                                        >  2:3806549/1‑150 (MQ=255)
             cATTGTACTGGAAGAAAACATTTTAAACATCAGGCAAATAACCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTa                                                                                                                                        <  1:2337705/150‑1 (MQ=255)
             cATTGTAATTGAAGAAAACATTTTAAACATCAGGCAAATAACAAATTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCTGGATGAGATTAAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCATCTTGTTTTCTa                                                                                                                                        <  2:2185709/150‑1 (MQ=255)
               ttGTACTGGAAGAAAACATTTTAAACATCAGGCAAATAACCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACt                                                                                                                                      >  1:591375/1‑150 (MQ=255)
                  tACTGGAAGAAAACATTTTAAACATCAGGCAAATAACCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACtttt                                                                                                                                   <  1:3680878/150‑1 (MQ=255)
                      ggAAGAAAACATTTTAAACATCAGGCAAATAACCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCtt                                                                                                                               >  2:944735/1‑150 (MQ=255)
                       gaagaaAACATTTTAAACATCAGGCAAATAACCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTa                                                                                                                              >  1:727560/1‑150 (MQ=255)
                        aagaaAACATTTTAAACATCAGGCAAATAACCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTaa                                                                                                                             <  2:2491749/150‑1 (MQ=255)
                         agaaAACATTTTAAACATCAGGCAAATAACCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAAc                                                                                                                            >  2:2504550/1‑150 (MQ=255)
                          gaaAACATTTTAAACATCAGGCAAATAACCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGGCCCATTTAAAGCACAAAACCCGCTCATAAGCGGGTTTTCTACTTTTTCTTAACg                                                                                                                           >  2:1225761/1‑150 (MQ=255)
                            aaaCATTTTAAACATCAGGCAAATAACCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTc                                                                                                                         >  1:2639742/1‑150 (MQ=255)
                            aaaCATTTTAAACATCAGGCAAATAACCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTc                                                                                                                         >  2:259936/1‑150 (MQ=255)
                             aaCATTTTAAACATCAGGCAAATAACCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCg                                                                                                                        >  2:454740/1‑150 (MQ=255)
                             aaCATTTTAAACATCAGGCAAATAACCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCg                                                                                                                        <  1:2404711/150‑1 (MQ=255)
                              aCATTTTAAACATCAGGCAAATAACCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTACCGTCgg                                                                                                                       >  1:1888262/1‑150 (MQ=255)
                              aCATTTTAAACATCAGGCAAATAACCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCgg                                                                                                                       <  2:4011168/150‑1 (MQ=255)
                              aCATTTTAAACATCAGGCAAATAACCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCgg                                                                                                                       <  1:3749525/150‑1 (MQ=255)
                                aTTTTAAACATCAGGCAAATAACCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGt                                                                                                                     >  1:3541514/1‑150 (MQ=255)
                                 ttttAAACATCAGGCAAATAACCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGta                                                                                                                    >  2:3833152/1‑150 (MQ=255)
                                        cATCAGGCAAATAACCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAg                                                                                                             <  1:1648899/150‑1 (MQ=255)
                                         aTCAGGCAAATAACCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGc                                                                                                            >  1:3358439/1‑150 (MQ=255)
                                               cAAATAACCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCg                                                                                                      <  2:618067/150‑1 (MQ=255)
                                                 aaTAACCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGtt                                                                                                    >  1:2223064/1‑150 (MQ=255)
                                                     aCCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGaaa                                                                                                >  1:1554279/1‑150 (MQ=255)
                                                       cAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGaaaaa                                                                                              <  2:3195499/150‑1 (MQ=255)
                                                        aaGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGaaaaaa                                                                                             <  1:3801203/150‑1 (MQ=255)
                                                        aaGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGaaaaaa                                                                                             >  1:4114353/1‑150 (MQ=255)
                                                        aaGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGAGGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGaaaaaa                                                                                             <  1:1916215/150‑1 (MQ=255)
                                                           tCACCAGCTAAATAATCAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATGTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAAttt                                                                                          <  2:2184108/150‑1 (MQ=255)
                                                             aCCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTa                                                                                        <  2:3779298/150‑1 (MQ=255)
                                                              ccAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTAt                                                                                       <  2:1143305/150‑1 (MQ=255)
                                                                aGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATcc                                                                                     >  2:201681/1‑150 (MQ=255)
                                                                aGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATcc                                                                                     >  2:696504/1‑150 (MQ=255)
                                                                   tAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCata                                                                                  >  1:457615/1‑150 (MQ=255)
                                                                     aataatAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATAtt                                                                                >  2:54/1‑150 (MQ=255)
                                                                     aataatAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATAtt                                                                                >  2:73/1‑150 (MQ=255)
                                                                      ataataAGTTAACAGAAATGAGTCCCGGGATGATAAACAACATTACCATTGACACATTTAAAGCACAAAACCCGCTAATCATAGTTAATTCTAATTTTACTTAACTTCTGTTATACAAAGCAAATCCATGAAAAAAATTTATCAatatat                                                                               >  2:1007535/1‑148 (MQ=255)
                                                                        aataaGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATAttttt                                                                             <  2:4127448/150‑1 (MQ=255)
                                                                        aataaGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCAATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCTGGTATAAAAAGCCCATCGTTGAAAAAATTTTATCCATAttttt                                                                             >  1:3947494/1‑150 (MQ=255)
                                                                             gTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACACGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGaaa                                                                        >  1:1055685/1‑150 (MQ=255)
                                                                                aaCAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAAt                                                                     <  1:2574941/150‑1 (MQ=255)
                                                                                aaCAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAAt                                                                     >  2:3445797/1‑150 (MQ=255)
                                                                                aaCAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAAt                                                                     >  2:162654/1‑150 (MQ=255)
                                                                                aaCAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAAt                                                                     >  1:3186367/1‑150 (MQ=255)
                                                                                aaCAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCAGCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAACAAATTTTATCCATATTTTTTGAAAAAt                                                                     >  2:1704434/1‑150 (MQ=255)
                                                                                 aCAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATg                                                                    >  1:2936085/1‑150 (MQ=255)
                                                                                    gACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCaa                                                                 <  2:2668018/150‑1 (MQ=255)
                                                                                      cATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAAc                                                               >  1:3032528/1‑150 (MQ=255)
                                                                                       aTGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTCTACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAAca                                                              >  2:105946/1‑150 (MQ=255)
                                                                                       aTGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAAca                                                              >  2:1241702/1‑150 (MQ=255)
                                                                                        tGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAAcat                                                             >  2:1165062/1‑150 (MQ=255)
                                                                                        tGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAAcat                                                             >  1:3086034/1‑150 (MQ=255)
                                                                                            tCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATg                                                         <  2:4213998/150‑1 (MQ=255)
                                                                                            tCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATg                                                         >  2:1439173/1‑150 (MQ=255)
                                                                                            gcccGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATg                                                         <  1:2512421/149‑1 (MQ=255)
                                                                                                  gATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAATTGCAAACATCATGTCGCCa                                                   <  1:3785885/150‑1 (MQ=255)
                                                                                                  gATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCa                                                   >  2:1267229/1‑150 (MQ=255)
                                                                                                  gATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCa                                                   >  2:2812952/1‑150 (MQ=255)
                                                                                                   aTGAGATTCAACATTACCATTGCCCCATTTAAAGCACAACACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCAt                                                  <  2:3580199/150‑1 (MQ=255)
                                                                                                   aTGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCAt                                                  <  2:2920240/150‑1 (MQ=255)
                                                                                                   aTGAGATTCAACATTACCATTGCCCCAATTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCAt                                                  <  2:3580362/150‑1 (MQ=255)
                                                                                                       gaTTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCATCTTc                                              >  1:2774175/1‑150 (MQ=255)
                                                                                                          tCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCATCTTCAGc                                           >  1:888527/1‑150 (MQ=255)
                                                                                                                ttACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCATCTTCAGCAAAAAt                                     >  1:1151248/1‑150 (MQ=255)
                                                                                                                tgaCCACTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCATCTTCAGCAAAAAt                                     <  1:2810910/148‑1 (MQ=255)
                                                                                                                    cATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCTTTGAAAAAATTATATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCATCTTCTGCAAAAATCAtt                                 >  2:3263592/1‑150 (MQ=255)
                                                                                                                    cATGGCCCCATCTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCATCTTCAGCAAAAATCAtt                                 <  1:3367718/150‑1 (MQ=255)
                                                                                                                     aTTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAAAGTCGGGTATACAAATCCCATAGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGAAAACATCATGTCGCCATCTTCAGCAAAAATAAttt                                >  1:3151206/1‑150 (MQ=255)
                                                                                                                         ccccATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCATCTTCAGCAAAAATCATTTAtct                            >  2:1642338/1‑150 (MQ=255)
                                                                                                                          ttaagaaaaaGTAGAAAACCCGCTGATGAGCGGGTTTTGTGCTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCATCTTCAGCAAAAATCATTTAtctc                           >  2:268577/8‑150 (MQ=38)
                                                                                                                          ttaagaaaaaGTAGAAAACCCGCTGATGAGCGGGTTTTGTGCTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCATCTTCAGCAAAAATCATTTAtctc                           >  2:3644052/8‑150 (MQ=38)
                                                                                                                          ttaagaaaaaGTAGAAAACCCGCTGATGAGCGGGTTTTGTGCTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCATCTTCAGCAAAAATCATTTAtctc                           >  2:3107450/8‑150 (MQ=38)
                                                                                                                          cccATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCATCTTCAGCAAAAATCATTTAtctc                           <  2:2272379/150‑1 (MQ=255)
                                                                                                                           ccATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCATCTTCAGCAAAAATCATTTATCTCg                          >  1:2048969/1‑150 (MQ=255)
                                                                                                                                  aaGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCATCTTCAGCAAAAATCATTTATCTCGTCACCtt                   >  1:1604946/1‑150 (MQ=255)
                                                                                                                                   aGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCATCTTCAGCAAAAATCATTTATCTCGTCACCTTc                  <  1:1165062/150‑1 (MQ=255)
                                                                                                                                   aGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCATCTTCAGCAAAAATCATTTATCTCGTCACCTTc                  >  2:1788028/1‑150 (MQ=255)
                                                                                                                                    gCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCTGGTATACAAAGCCCATCGTTGAAATAATTTTATCCATATTTTTTGAAAAATTCAAACATCATGTCGCCATCTTCAGCAAAAATCTTTTATGTCGTCACAttag                 >  2:3859090/1‑148 (MQ=255)
                                                                                                                                     tagaaaaCCCGCTGATGAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTCTCCATATTTTTTGAAAAATGCAAACATCATGTCGCCCTCTTCAGCAACAATCATTTATCTCGTCAGCTGCCt                >  2:2875397/4‑150 (MQ=255)
                                                                                                                                     cacaAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCATCTTCAGCAAAACTCATTTATCTCGTCACCTTccg                >  1:2677449/1‑149 (MQ=255)
                                                                                                                                      acaAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCATCTTCAGCAAAAATCATTTATCTCGTCACCTTCCTc               <  1:3063393/150‑1 (MQ=255)
                                                                                                                                      acaAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCATCTTCAGCAAAAATCATTTATCTCGTCACCTTCCTc               >  1:3617325/1‑150 (MQ=255)
                                                                                                                                       caAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCATCTTCAGCAAAAATCATTTATCTCGTCACCTTCCTCa              >  1:38754/1‑150 (MQ=255)
                                                                                                                                          aaCCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAATAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCATCTTCAGCAAAAATCATTTATCTCGTCACCTTCCTCAAtt           <  2:803099/150‑1 (MQ=255)
                                                                                                                                          aaCCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCATCTTCAGCAAAAATCATTTATCTCGTAACCTTCCTCAAtt           >  2:1041442/1‑150 (MQ=255)
                                                                                                                                              cGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCATCTTCAGCAAAAATCATTTATCTCGTCACCTTCCTCAATTgcgc       <  1:935015/150‑1 (MQ=255)
                                                                                                                                               gCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCATCTTCAGCAAAAATCATTTATCTCGTCACCTTCCTCAATTGCGCt      >  1:306001/1‑150 (MQ=255)
                                                                                                                                               gCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCATCTTCAGCAAAAATCATTTATCTCGTCACCTTCCTCAATTGCGCt      >  1:4138439/1‑150 (MQ=255)
                                                                                                                                                 tcatcaGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCATCTTCAGCAAAAATCATTTATCTCGTCACCTTCCTCAATTGCGCTTc    <  1:2502045/150‑1 (MQ=255)
                                                                                                                                                 tcatcaGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCATCTTCAGCAAAAATCATATATCTCGTCACCTGCCTCAATTGCGCTTc    >  2:2725831/1‑150 (MQ=255)
                                                                                                                                                  catcaGCGGGTTTTCTACTTTTTCTTAACGTCGGGTAGACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCATCTTCAGCAAAAATCATTTATCTCGTCACCTTCCTCAATTGCGCTTcc   <  1:2601225/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
TTGTGTTTAGGGACATTGTACTGGAAGAAAACATTTTAAACATCAGGCAAATAACCAAGTCACCAGCTAAATAATAAGTTAACAGACATGAGTCCCGGGATGAGATTCAACATTACCATTGCCCCATTTAAAGCACAAAACCCGCTCATCAGCGGGTTTTCTACTTTTTCTTAACGTCGGGTATACAAAGCCCATCGTTGAAAAAATTTTATCCATATTTTTTGAAAAATGCAAACATCATGTCGCCATCTTCAGCAAAAATCATTTATCTCGTCACCTTCCTCAATTGCGCTTCCG  >  NC_000913/1642224‑1642520

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: