Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,985,112 C→G 5.3% intergenic (‑43/+27) araG ← / ← araF L‑arabinose ABC transporter ATPase/L‑arabinose ABC transporter periplasmic binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,985,1120CG 5.3% 823.0 / 29.4 226intergenic (‑43/+27)araG/araFL‑arabinose ABC transporter ATPase/L‑arabinose ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base C (109/105);  new base G (5/7);  total (114/112)
Fisher's exact test for biased strand distribution p-value = 5.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

Only 100 of 239 total aligned reads displayed.

GAACCTGACCGGCATAGCAGTCAAAACTAATATCCGTCAGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCAACGTCTTTTGCTACCCAGTTGTAAAG  >  NC_000913/1984964‑1985261
                                                                                                                                                    |                                                                                                                                                     
gAACCTGACCGGCATAGCAGTCAAAACTAATATCCGTCAGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGcatca                                                                                                                                                      >  2:1692999/1‑150 (MQ=255)
  aCCTGACCGGCATAGCAGTCAAAACTAATATCCGTCAGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATg                                                                                                                                                    >  2:1271482/1‑150 (MQ=255)
   ccTGACCGGCATAGCAGTCAAAACTAATATCCGTCAGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGc                                                                                                                                                   >  1:385779/1‑150 (MQ=255)
      gACCGGCATAGCAGTCAAAACTAATATCCGTCAGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATGATGCaga                                                                                                                                                <  1:603721/150‑1 (MQ=255)
       aCCGGCATAGCAGTCAAAACTAATATCCGTCAGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCagag                                                                                                                                               <  2:2164113/150‑1 (MQ=255)
         cGGCATAGCAGTCAAAACTAATATCCGTCAGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAggg                                                                                                                                             <  1:3492320/150‑1 (MQ=255)
         cGGCATAGCAGTCAAAACTAATATCCGTCAGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAggg                                                                                                                                             <  1:1777857/150‑1 (MQ=255)
         cGGCATAGCAGTCAAAACTAATATCCGTCAGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCAGGCAGAggg                                                                                                                                             >  2:3318524/1‑150 (MQ=255)
          ggCATAGCAGTCAAAACTAATATCCGTCAGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATGATGCAGAgggg                                                                                                                                            <  2:511957/150‑1 (MQ=255)
          ggCATAGCAGTCAAAACTAATATCCGTCAGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATGATGCAGAgggg                                                                                                                                            <  2:3493665/150‑1 (MQ=255)
           gCATAGCAGTCAAAACTAATATCCGTCAGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGa                                                                                                                                           >  2:241694/1‑150 (MQ=255)
                  aGTCAAAACTAATATCCGTCAGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTc                                                                                                                                    <  1:3880527/150‑1 (MQ=255)
                  aGTCAAAACTAATATCCGTCAGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTc                                                                                                                                    <  1:4217488/150‑1 (MQ=255)
                   gTCAAAACTAATATCCGTCAGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTcc                                                                                                                                   <  1:4003681/150‑1 (MQ=255)
                    tCAAAACTAATATCCGTCAGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCg                                                                                                                                  <  1:4086918/150‑1 (MQ=255)
                    tCAAAACTAATATCCGTCAGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCg                                                                                                                                  <  2:2043974/150‑1 (MQ=255)
                      aaaaCTAATATCCGTCAGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGc                                                                                                                                >  2:1036774/1‑150 (MQ=255)
                                tCCGTCAGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAAttactta                                                                                                                      <  1:3359783/150‑1 (MQ=255)
                                   gTCAGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCg                                                                                                                   <  1:812878/150‑1 (MQ=255)
                                    tCAGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGc                                                                                                                  <  2:1213029/150‑1 (MQ=255)
                                    tCAGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGc                                                                                                                  <  2:1367966/150‑1 (MQ=255)
                                      aGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCt                                                                                                                >  2:301574/1‑150 (MQ=255)
                                        cgcCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTaa                                                                                                              >  2:4093985/1‑150 (MQ=255)
                                               aCGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCtttt                                                                                                       <  1:2386537/150‑1 (MQ=255)
                                               aCGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCtttt                                                                                                       <  2:317651/150‑1 (MQ=255)
                                               aCGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCtttt                                                                                                       <  1:3503941/150‑1 (MQ=255)
                                                cGCCGTGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGTGTTAGACTGTTGCATAACGTCTACGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTAACGAATAAACCttttt                                                                                                      >  2:1571492/1‑150 (MQ=255)
                                                 gCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCtttttt                                                                                                     >  2:132805/1‑150 (MQ=255)
                                                 gCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCtttttt                                                                                                     >  1:1656279/1‑150 (MQ=255)
                                                  ccGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTtc                                                                                                    <  1:258350/150‑1 (MQ=255)
                                                  ccGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTtc                                                                                                    <  1:3933526/150‑1 (MQ=255)
                                                    gggAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTtctc                                                                                                  >  2:3714829/1‑150 (MQ=255)
                                                    gggAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTtctc                                                                                                  >  2:2127695/1‑150 (MQ=255)
                                                       aaaCGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAg                                                                                               >  2:2858624/1‑150 (MQ=255)
                                                          cGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGACTTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGttc                                                                                            >  1:3333264/1‑150 (MQ=255)
                                                             tttACCGATGCCGCGAACTGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGttcttc                                                                                         <  2:455878/150‑1 (MQ=255)
                                                             tttACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGttcttc                                                                                         <  1:3427626/150‑1 (MQ=255)
                                                             tttACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGttcttc                                                                                         <  2:455944/150‑1 (MQ=255)
                                                             ttgaCCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGttcttc                                                                                         <  2:427935/147‑1 (MQ=255)
                                                                 ccGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTa                                                                                     >  2:730745/1‑150 (MQ=255)
                                                                   gATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTaaa                                                                                   <  2:1611283/150‑1 (MQ=255)
                                                                   gATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTaaa                                                                                   <  1:1902269/150‑1 (MQ=255)
                                                                   gATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTaaa                                                                                   <  2:708292/150‑1 (MQ=255)
                                                                   gATGCCGCGAAAAGACATATACGGTGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGAAACATGCAGAGGGTAAATTTTCCGGAAAAATACTTACCCCCTAAACCTATTTTCTCCAGTTCTTCATTaaa                                                                                   <  2:891675/150‑1 (MQ=255)
                                                                    aTGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAg                                                                                  >  2:2715302/1‑150 (MQ=255)
                                                                    aTGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAg                                                                                  >  1:1554238/1‑150 (MQ=255)
                                                                     tGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGt                                                                                 <  2:1886832/150‑1 (MQ=255)
                                                                      gCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGtt                                                                                <  2:1081605/150‑1 (MQ=255)
                                                                       ccGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTg                                                                               >  1:3735963/1‑150 (MQ=255)
                                                                        cgcgAAATGAGAGATACGGGGTCGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGGTCTTCTTTAAAGTTGt                                                                              >  2:2576232/1‑150 (MQ=255)
                                                                        cgcgAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGtttt                                                                              >  2:3906897/1‑148 (MQ=255)
                                                                        cgcgAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGt                                                                              >  2:2069174/1‑150 (MQ=255)
                                                                        cgcgAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGt                                                                              >  1:3204739/1‑150 (MQ=255)
                                                                         gcgAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGACTTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTc                                                                             >  1:2904272/1‑150 (MQ=255)
                                                                         gcgAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTc                                                                             >  1:1007838/1‑150 (MQ=255)
                                                                         gcgAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTc                                                                             <  1:2873483/150‑1 (MQ=255)
                                                                         gcgAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTc                                                                             >  1:1966159/1‑150 (MQ=255)
                                                                             aaTGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCAcgc                                                                         <  2:3928361/150‑1 (MQ=255)
                                                                                 agagaTACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGa                                                                     <  2:2473802/150‑1 (MQ=255)
                                                                                        cGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTAATTACCTCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTAc                                                              <  2:242359/150‑1 (MQ=255)
                                                                                          gggTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCa                                                            >  2:2933827/1‑150 (MQ=255)
                                                                                           ggTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCAc                                                           <  1:1117566/150‑1 (MQ=255)
                                                                                            gTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACg                                                          >  2:2384510/1‑150 (MQ=255)
                                                                                             tAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGt                                                         <  2:1961230/150‑1 (MQ=255)
                                                                                               gACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCg                                                       >  2:3089987/1‑150 (MQ=255)
                                                                                                  tGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGACTTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTATCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTa                                                    >  2:4183052/1‑150 (MQ=255)
                                                                                                  tGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTa                                                    >  1:2706013/1‑150 (MQ=255)
                                                                                                    ttGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAAc                                                  >  1:1605533/1‑150 (MQ=255)
                                                                                                    ttGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAAGTACTTACCGCCTAAACATTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGAACCACGTCGGTCAc                                                  >  2:188254/1‑150 (MQ=255)
                                                                                                      gCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATCACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACtt                                                <  1:2123752/150‑1 (MQ=255)
                                                                                                       cATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTc                                               >  2:2820042/1‑150 (MQ=255)
                                                                                                       cATAACGTCACCGTGAAACACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGCGGAATTTATCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTACCTTc                                               >  2:3237049/1‑150 (MQ=255)
                                                                                                          aaCGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTGTGCAGTTGTTGTTTAAAGTTGTCACGGGTGATCAGTACCACGTCGGTAAATTCGGt                                            >  1:861075/1‑150 (MQ=255)
                                                                                                           aCGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTa                                           >  2:1618666/1‑150 (MQ=255)
                                                                                                              tctcCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCACATTACTTACCGCCGAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGGAAAt                                        >  1:1463311/1‑150 (MQ=255)
                                                                                                                    tGAATCACTGGTCGTTCACACCCCCTCAGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAAATTCGGTAAATTTTGGc                                  >  1:1321015/1‑150 (MQ=255)
                                                                                                                     gAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCg                                 <  2:2607585/150‑1 (MQ=255)
                                                                                                                     gAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCg                                 <  2:663625/150‑1 (MQ=255)
                                                                                                                     gAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCg                                 <  1:137379/150‑1 (MQ=255)
                                                                                                                     gAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGGAAATTTTGGCg                                 >  1:1283791/1‑150 (MQ=255)
                                                                                                                       aTCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGt                               >  2:14415/1‑150 (MQ=255)
                                                                                                                         cACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTc                             <  2:1845727/150‑1 (MQ=255)
                                                                                                                            tGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCAAc                          <  1:3090153/150‑1 (MQ=255)
                                                                                                                                 gTTCACACCCCCTCGGCATCAGGCAGAGGGGAATTTTTCCGGCAAATTACTTCCCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCAACGTCtt                     <  2:2730759/150‑1 (MQ=255)
                                                                                                                                    cacaCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCAACGTCTTTTg                  >  1:3632160/1‑150 (MQ=255)
                                                                                                                                    cacaCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCAACGTCTTTTg                  >  1:2769891/1‑150 (MQ=255)
                                                                                                                                    cacaCCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCAACGTCTTTTg                  >  1:451229/1‑150 (MQ=255)
                                                                                                                                      cacCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGTCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTATTTAAAGTTGTCACGCGTGATCAGTACCACTTCGGTAACTTCTGTAAATTTTGGCTGTTCAACTTCTTTTGCt                >  2:824267/1‑150 (MQ=255)
                                                                                                                                      cacCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCAACGTCTTTTGCt                >  2:1475547/1‑150 (MQ=255)
                                                                                                                                       ttccccTCTGCATGATGCAGAGGGGGATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCAACGTCTTTTGCTa               >  2:1650897/3‑150 (MQ=255)
                                                                                                                                         ccccTCTGCATGATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTAACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCAACGTCTTTTGCTAcc             >  1:205521/1‑150 (MQ=255)
                                                                                                                                          cccTCTGCATGATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCAACGTCTTTTGCTAccc            >  1:2999067/1‑150 (MQ=255)
                                                                                                                                          cccTCTGCATGATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCAACGTCTTTTGCTAccc            >  1:2337294/1‑150 (MQ=255)
                                                                                                                                          cccTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAACCCTTTTTTCTCCAGGTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTCAGTTCGGTAAATTTTGGCGGTTCAACGTCTTTTGCTAccc            >  2:2536886/1‑150 (MQ=255)
                                                                                                                                           ccTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCAACGTCTTTTGCTACCCa           >  1:187238/1‑150 (MQ=255)
                                                                                                                                             tctGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCAACGTCTTTTGCTACCCAGt         >  2:489774/1‑150 (MQ=255)
                                                                                                                                                gCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCAACGTCTTTTGCTACCCAGTTGt      <  1:4093985/150‑1 (MQ=255)
                                                                                                                                                 catcatGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCAACGTCTTTTGCTACCCAGTTGTa     <  2:1007838/150‑1 (MQ=255)
                                                                                                                                                 catcatGAAGAGGGGAATTTTTCCCGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCAACGTCTTTTGCTACCCAGTTGTa     >  2:3933912/1‑150 (MQ=255)
                                                                                                                                                   tcatGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCAACGTCtt                     <  1:1105266/132‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GAACCTGACCGGCATAGCAGTCAAAACTAATATCCGTCAGCGCCTTAACGCCGGGAAACGTTTTACCGATGCCGCGAAATGAGAGATACGGGGTAGACTGTTGCATAACGTCTCCGTGAATCACTGGTCGTTCACACCCCCTCTGCATCATGCAGAGGGGAATTTTTCCGGCAAATTACTTACCGCCTAAACCTTTTTTCTCCAGTTCTTCTTTAAAGTTGTCACGCGTGATCAGTACCACGTCGGTAACTTCGGTAAATTTTGGCGGTTCAACGTCTTTTGCTACCCAGTTGTAAAG  >  NC_000913/1984964‑1985261

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: