Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,177,234 C→A 19.3% intergenic (‑30/+278) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,177,2340CA19.3% 420.8 / 21.1 199intergenic (‑30/+278)gatY/fbaBD‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
Reads supporting (aligned to +/- strand):  ref base C (65/82);  new base A (29/15);  total (102/97)
Fisher's exact test for biased strand distribution p-value = 1.57e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 203 total aligned reads displayed.

GTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGG  >  NC_000913/2177085‑2177383
                                                                                                                                                     |                                                                                                                                                      
gTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTTTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTc                                                                                                                                                        >  1:303349/1‑150 (MQ=255)
 gtGGCAGCGGTTTCTACCACCACGTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCa                                                                                                                                                       <  1:1293936/149‑1 (MQ=255)
    gCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCaaaa                                                                                                                                                    >  2:3808935/1‑150 (MQ=255)
     cAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGACTATTGAATGCCGGAACCGCATAACCGCCGCGCTGGGCGTTGTTCAGCATCTGCGTTGTCGATACAACGTACATTTTCATATCCTGTCGTTTGGTTTCGATTTGCAAat                                                                                                                                                   >  1:2288047/1‑150 (MQ=255)
     cAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAat                                                                                                                                                   <  2:3746504/150‑1 (MQ=255)
     cAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGAGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGATTTCAAAat                                                                                                                                                   >  1:2260312/1‑150 (MQ=255)
      aGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAata                                                                                                                                                  >  2:206991/1‑150 (MQ=255)
      aGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCAGACGTTTGTTTTCGATTTAAAAata                                                                                                                                                  >  1:2362152/1‑150 (MQ=255)
          gTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAAt                                                                                                                                              <  2:2524676/150‑1 (MQ=255)
            ttCTACCACCACTAGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGa                                                                                                                                            <  1:4029170/150‑1 (MQ=255)
             tCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAATGaa                                                                                                                                           >  1:1054402/1‑150 (MQ=255)
              cTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGaaa                                                                                                                                          <  1:3619632/150‑1 (MQ=255)
              cTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAATTaaa                                                                                                                                          >  1:1351212/1‑150 (MQ=255)
               tACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGATTTCAAAATATAATTaaaa                                                                                                                                         >  2:879069/1‑149 (MQ=255)
               tACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGAATTAAAAATATAATTAAat                                                                                                                                         >  1:4093482/1‑150 (MQ=255)
               tACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAat                                                                                                                                         <  1:3175491/150‑1 (MQ=255)
               tACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAat                                                                                                                                         >  2:3881312/1‑150 (MQ=255)
                accaccACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAatt                                                                                                                                        >  1:2846397/1‑150 (MQ=255)
                accaccACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAatt                                                                                                                                        >  1:2506652/1‑150 (MQ=255)
                 ccaccaCTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAatta                                                                                                                                       >  2:1075874/1‑150 (MQ=255)
                 ccaccaCTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAATTAAaata                                                                                                                                       >  1:1211996/1‑150 (MQ=255)
                  caccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTCAAAATATAATGAAattat                                                                                                                                      >  2:3708562/1‑150 (MQ=255)
                   accacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATTAAattatt                                                                                                                                     >  2:3640140/1‑150 (MQ=255)
                   accacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAattatt                                                                                                                                     <  1:341305/150‑1 (MQ=255)
                     cacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATTAAATTAtttt                                                                                                                                   >  1:2690383/1‑149 (MQ=255)
                     cacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAATTAAATTATTTg                                                                                                                                   >  1:2690307/1‑150 (MQ=255)
                      acTTGCATCGTTTCGAGATTGTGAAAATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTCGAATTCAAAATATAATGAAATTAttttt                                                                                                                                  >  2:2936402/1‑148 (MQ=255)
                        ttGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGttt                                                                                                                                <  2:1419732/150‑1 (MQ=255)
                           cATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTa                                                                                                                             <  2:760438/150‑1 (MQ=255)
                            aTCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTaa                                                                                                                            <  2:1187457/150‑1 (MQ=255)
                             tCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATTACCGCCGCGCTGTGCGTTGTTCAGCATCTGGTTTGTCGATACCACGTACTTTTTCATATGCTGTCGTTTTTTTTTGATTTGAAAATATATTTTAAttttttttttttttt                                                                                                                           >  2:1585222/1‑147 (MQ=255)
                                tttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAatat                                                                                                                        <  1:2247912/150‑1 (MQ=255)
                                  tCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCg                                                                                                                      <  1:1827302/150‑1 (MQ=255)
                                  tCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCg                                                                                                                      <  2:2177982/150‑1 (MQ=255)
                                  tCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCg                                                                                                                      <  1:3198989/150‑1 (MQ=255)
                                  tCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCg                                                                                                                      <  2:556109/150‑1 (MQ=255)
                                   cGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATAga                                                                                                                     >  2:1235657/1‑150 (MQ=255)
                                   cGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATAACCTGTCGTTTGTTTTCGAATTCAAAATAAAAATAAAAAAATTGTTTTTAAAAATCga                                                                                                                     >  2:399846/1‑150 (MQ=255)
                                    gagaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCgag                                                                                                                    <  1:2823407/150‑1 (MQ=255)
                                      gaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATTAAATTATTTGTTTTTAAATATCGAGAt                                                                                                                  >  1:715766/1‑150 (MQ=255)
                                      gaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATTAAATAATTTTTTTTTAAATAACgaggt                                                                                                                  >  1:484515/1‑148 (MQ=255)
                                      gaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGGTTTTAAATATCGAGAt                                                                                                                  >  2:2834437/1‑150 (MQ=255)
                                           tgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGa                                                                                                             <  1:799768/150‑1 (MQ=255)
                                           tgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGa                                                                                                             <  1:3696344/150‑1 (MQ=255)
                                            gAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGAt                                                                                                            <  2:240355/150‑1 (MQ=255)
                                               tatTGAATGCCGGAACCGCATAACCGCCCCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAAAGGAATTAGTTGTTTTTAAATATCGAGATAACGATcac                                                                                                         >  1:2465828/1‑150 (MQ=255)
                                                 ttGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACaa                                                                                                       >  1:3144226/1‑150 (MQ=255)
                                                  tGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCATCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCTTTTGTTTTCGATTTCAAAATATAATGTAATTATTTGTTTTTAAATATCGAGATAACGATCACaaa                                                                                                      <  1:2178961/150‑1 (MQ=255)
                                                   gAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTCAAAAAATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACaaaa                                                                                                     >  2:3345630/1‑150 (MQ=255)
                                                    aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACaaaaa                                                                                                    <  1:1786568/150‑1 (MQ=255)
                                                         cGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAATATAATGTAAATATTTGTTTTTAAATATAGAGATAACGATCACCGAAAAGACa                                                                                              >  1:3064950/1‑150 (MQ=255)
                                                           gAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATAGAGATAACGATCACAAAAACGACAat                                                                                            >  1:3476624/1‑150 (MQ=255)
                                                               cGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGa                                                                                        <  2:64621/150‑1 (MQ=255)
                                                               cGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCGGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAATGAAAGTATTTGTTGTTAAATATCGAGATACCGATCACCAAAACCACAAGATGa                                                                                        >  1:2670760/1‑150 (MQ=255)
                                                                gCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCATAAAATAAATAAAATAATTTTTTTTAAATATAGAGATAAAGATAACAAAAACGACAAAAAGaa                                                                                       >  2:1928121/1‑150 (MQ=255)
                                                                gCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAATTAAAATAATTTTTTTTAAATATAGAGATAAAGATAACAAAAAAGAAAATATTaa                                                                                       >  1:4142921/1‑150 (MQ=255)
                                                                gCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTAAAAATATAATTAAAATATTTTTTTTTAAATATCGAGATAACGAAAAAAAAAAAGACAAAATGaa                                                                                       >  1:2282739/1‑150 (MQ=255)
                                                                        gccgcGCTGTGCGTTGTTCCGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTc                                                                               <  2:1456461/150‑1 (MQ=255)
                                                                         ccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAAATAAAGAAAATATTTTTTTTTAAATATCGAGATAAAGATCACAAAAACGACAAAATGAAAAATATTCg                                                                              >  2:1318882/1‑150 (MQ=255)
                                                                           gcgcTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCgag                                                                            <  2:503670/150‑1 (MQ=255)
                                                                           gcgcTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCgag                                                                            <  2:676855/150‑1 (MQ=255)
                                                                            cgcTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTAGATTTTAAAATATAATTTAAATATTTTTTTTTTAATATATATTTAAAGATAAAAAAAAAGAAAATATTAAAaatattatttg                                                                           >  1:1718297/1‑145 (MQ=255)
                                                                            cgcTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGTTTTCAAAAAATAATTAAATTATTTTTTTTTAAATATAGAGATAAAGATCACAAAAAAGACAATATGTAAATTATTAgagg                                                                           >  1:595826/1‑150 (MQ=255)
                                                                             gcTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCgagga                                                                          <  2:1051390/150‑1 (MQ=255)
                                                                                    gtttttCAGAATCTGCTTTGTAGATACCCCGTACATTTTCATATCCTGTTTTTTGTTTTCTATTTCAAAATATATTGAAATTATTTTTTTTTAAATATCGAGATATAGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGaatg                                                                   <  2:4105902/150‑3 (MQ=255)
                                                                                    gttgttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTCTATTAAAAAATATAATCAAATTATTTGTTTTTAAATATCGAGATAACTATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAg                                                                   <  1:2341982/150‑1 (MQ=255)
                                                                                     ttgttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTGGATTTAAAAATAAAAATAAAATATTTTTTTTTAAATATAGAGATAACCATCACAAAAAACACAAAAAGAAAAATATTCGAGGAGTGaaatg                                                                  >  1:231497/1‑148 (MQ=255)
                                                                                     ttgttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAATAAAATTTAAATATTTTTTTTTAAATAAAGATAAAAAGATGAAAAAAAAGACAAAAATAAAAATAATAGTGGAGTGtaatg                                                                  >  1:1318983/1‑148 (MQ=255)
                                                                                         tcagattCTGCTTTTTCGATCCCACTAACATTTTCATATCCTTTGGTTTTTTTTCTATTAAAAAAAATATTTAATTTTTTTTTTTTTAATTATCTATATAACTATCAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaaa                                                              <  1:3881312/144‑1 (MQ=255)
                                                                                          cAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTTGATTTTAAAAAAAAATTAAAATAATTTTTTTTTAAAATAGAGTTAAAGGAAGCAAAAAAAACAATAATAAAAATATTTAATGAGTTGAAGGGaaaa                                                             >  1:1210509/1‑150 (MQ=255)
                                                                                          cAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTGGATTTGAAAAAATAAATAAAAAATTTTTTTTTTAAAATATAGATAAACATCACAAAAAACACAAAAATGAAAATATTAGAGGAGTGTAAAGCacaa                                                             >  1:1953565/1‑150 (MQ=255)
                                                                                           aGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAAAAAAATTAAAATATATTTTTTTAAATATAGAGATAACGATAAAAAAAAACAAAAAAAGAAAAATAATTGTGGTGTGGAAGGGcaaaa                                                            >  1:2385951/1‑150 (MQ=255)
                                                                                             aaTCTGCTTTGTCTATACCACGTACTTTTCCATCTCCTGTCTTTTTTTTTCTATTTAAAAATATAATTAAATTATTTGTTTTTAAATTTCGATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaaaaaaa                                                          <  1:3153786/149‑1 (MQ=255)
                                                                                                 tGCTTTGTCTATACCACTTACATTTTCATATCAGGTCTTTTTTTTTATATTTAAAATTATATTTAAATTATTTGTTTTTAAATATCTATATAACTATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGc                                                      <  2:3002135/150‑1 (MQ=255)
                                                                                                 tGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTAGAGGAGTGAAAGGCAAAAAAAAGGc                                                      >  2:2336802/1‑150 (MQ=255)
                                                                                                      tGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTccc                                                 <  2:1351212/150‑1 (MQ=255)
                                                                                                          gATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATa                                             <  2:1054402/150‑1 (MQ=255)
                                                                                                           aTACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAATGAAATTATTTGTTTTTAAATATCGATATAAAGATCAAAAAAACGACAATATGAAAATTATTAGAGGAGTGAAATGCAAAAAAACGGCCTCCCGATAg                                            >  2:3435969/1‑150 (MQ=255)
                                                                                                           aTACCACGTACAGTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAAGGCCTCCCCATAg                                            >  2:3085509/1‑150 (MQ=255)
                                                                                                             acaacttACTTTTGCATTTCCTTTCTTTTTTTTTATTTTAAAAAATATAATTAATTTTTTTTTTTTTAAATATCTATATAACGAACAAAAAAACGAAAATATTAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAggg                                          <  1:405155/144‑1 (MQ=255)
                                                                                                             aCCACGTACATTTTCATATCCTGTCTTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAggg                                          <  2:1707939/150‑1 (MQ=255)
                                                                                                             aCCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAggg                                          <  2:3544288/150‑1 (MQ=255)
                                                                                                                      aTTTTCATATCCTGTCGTTTTTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTTAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTGGAGGGGGGAAAGGCAAAAAAACGGCCTCCCGATAGGGACGCCGTAg                                 >  2:2891753/1‑150 (MQ=255)
                                                                                                                        tttCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGCTTGGGAAGCCGTAGCa                               >  2:3493875/1‑150 (MQ=255)
                                                                                                                          tCATATCCTGTGGTTTGTTTTCGATTTCAAAATATACTGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAACCGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCaaa                             <  2:3322964/150‑1 (MQ=255)
                                                                                                                             tatCCTGTCGTTTGTTTTCGATTTCAAAATATAAAGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAAAGACAATATGAAAATTATTAGAGGAGTGAAAGGCAAAAAAAAGGCCACCCGATAGGGAAGCCGTAGCAAAGTg                          >  2:225545/1‑150 (MQ=255)
                                                                                                                             tatCCTGTCGTTTGTTTTCGATTTAAAAATATAAATAAATTATTTTTTTTTAAATAACGAGATAAAGATAACAAAAAAGACAAAAAGAAAATTATTCGGGGAGTGAAAGGCAAAAAAAAGGCCTCCCGATCGGGAAGCCGTAGCAAAGTg                          >  2:1905923/1‑150 (MQ=255)
                                                                                                                               tCCTTTCTTTTTTTTTCTATTAAAAAATATAATTAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTgcg                        <  1:4248940/150‑1 (MQ=255)
                                                                                                                                ccTGGCTTTTGTTTTCGATTTCAAAATATAATCAAATTTTTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTgcgc                       <  1:1307086/150‑1 (MQ=255)
                                                                                                                                  tGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCgt                     >  2:1267368/1‑150 (MQ=255)
                                                                                                                                       ttttttttCTATTTCAAAATATAATTAAATTTTTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGtttt                <  1:900084/150‑1 (MQ=255)
                                                                                                                                       ttttttttATATTTAAAAATATAATTAAATTATTTTTTTTTAAATATCGAGATAACGAACACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGtttt                <  1:3345630/150‑1 (MQ=255)
                                                                                                                                           ttttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATg            <  2:3476624/150‑1 (MQ=255)
                                                                                                                                            tttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGc           >  2:4245487/1‑150 (MQ=255)
                                                                                                                                            tttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGc           <  2:2017154/150‑1 (MQ=255)
                                                                                                                                            tttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGc           <  2:741288/150‑1 (MQ=255)
                                                                                                                                             ttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGcc          >  2:3822869/1‑150 (MQ=255)
                                                                                                                                              tCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCg         >  2:96209/1‑150 (MQ=255)
                                                                                                                                                gATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGa       >  1:1564761/1‑150 (MQ=255)
                                                                                                                                                    acAAAATATAATTAAATTATTGGTTTTTAAATATCGAGATACCGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCg   <  1:3593958/149‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
GTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGG  >  NC_000913/2177085‑2177383

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: