Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,177,245 G→T 19.5% intergenic (‑41/+267) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,177,2450GT19.5% 450.7 / 23.1 209intergenic (‑41/+267)gatY/fbaBD‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
Reads supporting (aligned to +/- strand):  ref base G (77/77);  new base T (30/18);  total (107/102)
Fisher's exact test for biased strand distribution p-value = 1.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 211 total aligned reads displayed.

TTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATAC  >  NC_000913/2177097‑2177394
                                                                                                                                                    |                                                                                                                                                      
ttCTACCACCACTAGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGa                                                                                                                                                       <  1:4029170/150‑1 (MQ=255)
 tCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGaa                                                                                                                                                      <  1:3460350/150‑1 (MQ=255)
 tCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAATGaa                                                                                                                                                      >  1:1054402/1‑150 (MQ=255)
  cTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGaaa                                                                                                                                                     <  1:3619632/150‑1 (MQ=255)
  cTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAATTaaa                                                                                                                                                     >  1:1351212/1‑150 (MQ=255)
   tACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGAATTAAAAATATAATTAAat                                                                                                                                                    >  1:4093482/1‑150 (MQ=255)
   tACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATTAAat                                                                                                                                                    >  1:1525830/1‑150 (MQ=255)
   tACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAat                                                                                                                                                    >  2:3881312/1‑150 (MQ=255)
   tACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAATGAAat                                                                                                                                                    >  2:2201239/1‑150 (MQ=255)
    accaccACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAatt                                                                                                                                                   >  1:2846397/1‑150 (MQ=255)
    accaccACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAatt                                                                                                                                                   >  1:2506652/1‑150 (MQ=255)
     ccaccaCTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAatta                                                                                                                                                  >  2:1075874/1‑150 (MQ=255)
     ccaccaCTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAATTAAaata                                                                                                                                                  >  1:1211996/1‑150 (MQ=255)
      caccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTCAAAATATAATGAAattat                                                                                                                                                 >  2:128637/1‑150 (MQ=255)
      caccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTCAAAATATAATGAAattat                                                                                                                                                 >  2:3708562/1‑150 (MQ=255)
      caccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTAAAAAAAAAATTAAattat                                                                                                                                                 >  1:462016/1‑150 (MQ=255)
       accacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTTCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAattatt                                                                                                                                                <  1:3402520/150‑1 (MQ=255)
       accacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATTAAattatt                                                                                                                                                >  2:3640140/1‑150 (MQ=255)
         cacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATTAAATTAtttt                                                                                                                                              >  1:2690383/1‑149 (MQ=255)
         cacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAATTAAATTATTTg                                                                                                                                              >  1:2690307/1‑150 (MQ=255)
            ttGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGttt                                                                                                                                           >  1:2422006/1‑150 (MQ=255)
            ttGCATCGTTTCGAGATTGTGAAAATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAATGAAAATATtttttt                                                                                                                                           >  2:1198634/1‑150 (MQ=255)
                 tCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATTACCGCCGCGCTGTGCGTTGTTCAGCATCTGGTTTGTCGATACCACGTACTTTTTCATATGCTGTCGTTTTTTTTTGATTTGAAAATATATTTTAAttttttttttttttt                                                                                                                                      >  2:1585222/1‑147 (MQ=255)
                   gTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAata                                                                                                                                    >  2:1431445/1‑150 (MQ=255)
                     ttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATc                                                                                                                                  <  2:3426602/150‑1 (MQ=255)
                     ttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAATATAATTAAATTATTTTTTTTTAAatata                                                                                                                                  >  1:3036538/1‑149 (MQ=255)
                      tCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCg                                                                                                                                 <  2:553535/150‑1 (MQ=255)
                      tCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCg                                                                                                                                 <  2:1412347/150‑1 (MQ=255)
                      tCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCg                                                                                                                                 <  1:1827302/150‑1 (MQ=255)
                      tCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCg                                                                                                                                 <  2:556299/150‑1 (MQ=255)
                      tCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCg                                                                                                                                 <  2:1951170/150‑1 (MQ=255)
                       cGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATAACCTGTCGTTTGTTTTCGAATTCAAAATAAAAATAAAAAAATTGTTTTTAAAAATCga                                                                                                                                >  2:399846/1‑150 (MQ=255)
                        gagaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCgag                                                                                                                               <  1:2823407/150‑1 (MQ=255)
                          gaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATTAAATTATTTGTTTTTAAATATCGAGAt                                                                                                                             >  1:715766/1‑150 (MQ=255)
                          gaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGGTTTTAAATATCGAGAt                                                                                                                             >  2:2834437/1‑150 (MQ=255)
                               tgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGa                                                                                                                        <  1:799768/150‑1 (MQ=255)
                               tgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGa                                                                                                                        <  1:3696344/150‑1 (MQ=255)
                                       gAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAAAGATCACaaaa                                                                                                                >  2:3345490/1‑150 (MQ=255)
                                       gAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACaaaa                                                                                                                >  1:3163446/1‑150 (MQ=255)
                                               gAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATAGAGATAACGATCACAAAAACGACAat                                                                                                        >  1:3476624/1‑150 (MQ=255)
                                                aaCCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAAAGAAATTATTTGTTTTTAAATATAGAGATAACGATAAAAAAAACGACaaaa                                                                                                       >  2:3798971/1‑148 (MQ=255)
                                                   cGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCGGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAATGAAAGTATTTGTTGTTAAATATCGAGATACCGATCACCAAAACCACAAGATGa                                                                                                   >  1:2670760/1‑150 (MQ=255)
                                                    gCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTAAAAATATAATTAAAATATTTTTTTTTAAATATCGAGATAACGAAAAAAAAAAAGACAAAATGaa                                                                                                  >  1:2282739/1‑150 (MQ=255)
                                                              cgctgtgtgCTTTGTTCATCATCTGCTTTGTCTATACCACGTACATTTTCATATCCTGTCGTTTTTTTTCTTTTTAAATATATAATCAATTTTTTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCga                                                                                        <  1:2250643/145‑1 (MQ=255)
                                                               gcgcTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACTTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCgag                                                                                       <  2:676780/150‑1 (MQ=255)
                                                                cgcTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGTTTTCAAAAAATAATTAAATTATTTTTTTTTAAATATAGAGATAAAGATCACAAAAAAGACAATATGTAAATTATTAgagg                                                                                      >  1:595826/1‑150 (MQ=255)
                                                                 gcTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCgagga                                                                                     <  2:4131494/150‑1 (MQ=255)
                                                                 gcTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCgagga                                                                                     <  2:1051390/150‑1 (MQ=255)
                                                                  cTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGATTTAAAAATATAATTAAAATATTTTTTTTTAAAAAAAGAGATAAAGATCACAAAAAAGACAAAATTAAAAATATTAgaggag                                                                                    >  2:749641/1‑150 (MQ=255)
                                                                         ttgttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAATAAAATTTAAATATTTTTTTTTAAATAAAGATAAAAAGATGAAAAAAAAGACAAAAATAAAAATAATAGTGGAGTGtaatg                                                                             >  1:1318983/1‑148 (MQ=255)
                                                                            ttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAAAGACAATATGAAAATTATTCGAGGAGTGGAAGGCaa                                                                          >  2:3455765/1‑150 (MQ=255)
                                                                             tcagattCTGCTTTTTCGATCCCACTAACATTTTCATATCCTTTGGTTTTTTTTCTATTAAAAAAAATATTTAATTTTTTTTTTTTTAATTATCTATATAACTATCAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaaa                                                                         <  1:3881312/144‑1 (MQ=255)
                                                                             tCAGCATCTGCTTTGTCGATACCCCTTACTTTTTCATATCCTGTCTTTTTTTTTGTTTTTAAAAATATAATTAATTTTTTTTTTTTTAAATATCTAGATAACGAACACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaaa                                                                         <  1:1951978/150‑1 (MQ=255)
                                                                             tCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTGAAAAAATAATGAAAATAATTTTTTTTTAATATAGTGATAACGATCACAAAAAACACAATAATGAAAATAATCGAGGGGTGGAAgtgaaa                                                                         >  1:3145508/1‑145 (MQ=255)
                                                                              cAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTTGATTTTAAAAAAAAATTAAAATAATTTTTTTTTAAAATAGAGTTAAAGGAAGCAAAAAAAACAATAATAAAAATATTTAATGAGTTGAAGGGaaaa                                                                        >  1:1210509/1‑150 (MQ=255)
                                                                               aGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTAAAAAAATAAAGAAATTATTTTTTTTTAAAAAACGAGATAACGATCAAAAAAAAAAAAAAAATAAAATTATTATAGGGGTTTAAGTCaaaaa                                                                       >  1:3179087/1‑150 (MQ=255)
                                                                                   tCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACg                                                                   <  2:1327393/150‑1 (MQ=255)
                                                                                     tGCTTTGTCTATACCACTTACATTTTCATATCAGGTCTTTTTTTTTATATTTAAAATTATATTTAAATTATTTGTTTTTAAATATCTATATAACTATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGc                                                                 <  2:3002135/150‑1 (MQ=255)
                                                                                            tCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTGGTTTTGAAAATATAATTTAATTATTTGTTTTTTAATATCGTGTTAACGTTCACAAAAACGTCAATATGTTAATTTTTGGGGGTGTGTAAGGGAAAAAATAGGGCTCCCgt                                                          >  2:234912/1‑149 (MQ=255)
                                                                                            tCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCAAAAAAACGACAATATTAAAATTATTCGAGGAGTGAAAGGCAAAAAAAAGGCCTcccca                                                          >  2:2050497/1‑148 (MQ=255)
                                                                                              gaaaCCACTTACATTTTCATATCGTTTCGTTTGTTTTCTATTACAAAATATAATTAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGGAAGGCAAAAAAACGGCCTCCCGATa                                                        <  1:2358728/147‑1 (MQ=255)
                                                                                              gATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATa                                                        <  2:1054402/150‑1 (MQ=255)
                                                                                              gATACCACGTAATTTTTATTATCCTGTCGTTTTTTTTCTATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGTGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATa                                                        <  2:462016/150‑1 (MQ=255)
                                                                                               aTACCACGTACAGTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAAGGCCTCCCCATAg                                                       >  2:3085509/1‑150 (MQ=255)
                                                                                                 acaacttACTTTTGCATTTCCTTTCTTTTTTTTTATTTTAAAAAATATAATTAATTTTTTTTTTTTTAAATATCTATATAACGAACAAAAAAACGAAAATATTAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAggg                                                     <  1:405155/144‑1 (MQ=255)
                                                                                                 aCCACGTACATTTTCATATCCTGTCTTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAggg                                                     <  2:1707939/150‑1 (MQ=255)
                                                                                                 aCCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAggg                                                     <  2:3544288/150‑1 (MQ=255)
                                                                                                   cACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGaa                                                   <  2:1916850/150‑1 (MQ=255)
                                                                                                   cACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGaa                                                   <  2:330509/150‑1 (MQ=255)
                                                                                                         ctttttCATAGCCTGTCGTTTGTTTTCTTTTTAAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACTATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCTTCCCTATCGGGAGGCCGtt                                             <  1:2201239/148‑2 (MQ=255)
                                                                                                          aTTTTCATATCCTGTCGTTTTTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTTAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTGGAGGGGGGAAAGGCAAAAAAACGGCCTCCCGATAGGGACGCCGTAg                                            >  2:2891753/1‑150 (MQ=255)
                                                                                                          aTTTTCATATCCTGTCGTTTGTTTTCGTTTTGAAAATATAATGAAATTTTTTGTTTTTTTATTTGGAGTTTAAGTTGACAAAATCGAGAATTTGTTAATTTTTGGTGGTGTTTAATGCATAAATTGGGCCTGCCGATTGGGTAGGCGTAg                                            >  2:707128/1‑150 (MQ=255)
                                                                                                          aTTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAg                                            >  2:2473788/1‑150 (MQ=255)
                                                                                                            tttCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGCTTGGGAAGCCGTAGCa                                          >  2:3493875/1‑150 (MQ=255)
                                                                                                                    ccTGGCTTTTGTTTTCGATTTCAAAATATAATCAAATTTTTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTgcgc                                  <  1:1307086/150‑1 (MQ=255)
                                                                                                                          gtttgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCACCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGttt                            >  1:1647082/1‑150 (MQ=255)
                                                                                                                           tttgtttTCGATTTCAAAATATAATTAATTTTTTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGtttt                           <  1:3345490/150‑1 (MQ=255)
                                                                                                                           tttgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGtttt                           >  1:2320810/1‑150 (MQ=255)
                                                                                                                           tttgtttACTATTAAAAAAAAAAATCAAATTAATTTTTTAAAAATATCCATATAACCAAAAAAAAAACGACAATATTAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGtttt                           <  1:3774752/150‑1 (MQ=255)
                                                                                                                            ttgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGttttt                          <  2:1252834/150‑1 (MQ=255)
                                                                                                                             tgtttTAGTTTTAATAATTTTTTGAAATTTTTTGTTTTTTAATTTGTTTATATCTATCACAACAACTAGAATATGAAAATTATTCGAGGATTGAAAGGCAAAAAAACGGCCTCCAGATTGGGTAGCCGTAGCAAAGTGCGCGTGTTTTTa                         <  1:2302650/150‑1 (MQ=255)
                                                                                                                              gaataccaTTTAAAAATAAAATCAAATTATTTGATTAAAAATATCGAGAAAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTAt                        <  2:2323036/143‑1 (MQ=255)
                                                                                                                                tttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGc                      >  2:2142454/1‑150 (MQ=255)
                                                                                                                                 ttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGcc                     >  2:3822869/1‑150 (MQ=255)
                                                                                                                                 ttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGcc                     >  1:4217149/1‑150 (MQ=255)
                                                                                                                                  tCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCg                    >  2:96209/1‑150 (MQ=255)
                                                                                                                                       ttCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGc               >  1:2133854/1‑150 (MQ=255)
                                                                                                                                        tCAAAATATAATGAAATTTTTTGTTTTTAAATATCGAGATAACTATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCg              <  2:817134/150‑1 (MQ=255)
                                                                                                                                         cAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCGCCCGGTAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCgg             >  1:1750473/1‑150 (MQ=255)
                                                                                                                                          aaaaTATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgt            >  2:3578976/1‑150 (MQ=255)
                                                                                                                                          aaaaTATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgt            >  1:2494304/1‑150 (MQ=255)
                                                                                                                                          aaaaTATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgt            >  1:1576680/1‑150 (MQ=255)
                                                                                                                                           aaaTATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgtg           >  2:1503887/1‑150 (MQ=255)
                                                                                                                                             atatAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGACGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTa         >  1:907085/1‑150 (MQ=255)
                                                                                                                                             atatAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTa         >  2:2981371/1‑150 (MQ=255)
                                                                                                                                             atatAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTa         <  1:811347/150‑1 (MQ=255)
                                                                                                                                              tataATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTaa        <  2:1902931/150‑1 (MQ=255)
                                                                                                                                                taATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAAc      >  1:3581019/1‑150 (MQ=255)
                                                                                                                                                taATCAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAAc      <  1:3965561/150‑1 (MQ=255)
                                                                                                                                                    gAAATTATTTGTATTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATAc  <  1:320606/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                      
TTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATAC  >  NC_000913/2177097‑2177394

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: