Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,972,653 A→T 6.2% intergenic (+16/+16) tas → / ← lplT putative NADP(H)‑dependent aldo‑keto reductase/lysophospholipid transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,972,6530AT 6.2% 983.1 / 37.8 275intergenic (+16/+16)tas/lplTputative NADP(H)‑dependent aldo‑keto reductase/lysophospholipid transporter
Reads supporting (aligned to +/- strand):  ref base A (142/116);  new base T (5/12);  total (147/128)
Fisher's exact test for biased strand distribution p-value = 4.67e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01

Only 100 of 291 total aligned reads displayed.

CCAGCACTCTGCTGGGCGCAACCACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCATCAACAACATGG  >  NC_000913/2972504‑2972802
                                                                                                                                                     |                                                                                                                                                     
 cAGCACTCTGCTGGGCGCAACCACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTaaa                                                                                                                                                      >  1:4100861/1‑150 (MQ=255)
  aGCACTCTGCTGGGCGCAACCACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTaaaa                                                                                                                                                     <  2:4221946/150‑1 (MQ=255)
   gCACTCTGCTGGGCGCAACCACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAAc                                                                                                                                                    <  2:2197432/150‑1 (MQ=255)
    cACTCTGCTGGGCGCAACCACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAAcc                                                                                                                                                   >  1:1999416/1‑150 (MQ=255)
    cACTCTGCTGGGCGCAACCACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAAcc                                                                                                                                                   >  1:2176936/1‑150 (MQ=255)
     aCTCTGCTGGGCGCAACCACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCg                                                                                                                                                  >  2:3646214/1‑150 (MQ=255)
      ctctGCTGGGCGCAACCACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTTTAACCgg                                                                                                                                                 <  1:2754246/150‑1 (MQ=255)
       tctGCTGGGCGCAACCACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGc                                                                                                                                                >  1:82668/1‑150 (MQ=255)
        ctgctgGGCGCAACCACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCg                                                                                                                                               <  2:4002259/150‑1 (MQ=255)
         tggtgGGCGCAACCACGATGGATCAGCTGAAAACTAACATCGAATGTTTGCATCTGGTGTTAAGCGAAGACTTTTTAGCTTAAATTGATGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTTTAACCGGCGt                                                                                                                                              <  1:3386389/147‑1 (MQ=255)
         tgctgGGCGCAACCACGATGGATCAGCTTAAAACTAACATCGAATGTTTGCATCTGGAGTTAAGCGAAGACTTATTAGCTTAAATTGAAGCGGTGCATCAGTTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTTTAACCGGCGt                                                                                                                                              <  1:1587995/150‑1 (MQ=255)
         tgctgGGCGCAACCACGATGGATCAGCTGAAAACTAACATCGAAATTTTGCATCTGTAGTTAAGCTAATACTTATTAGCTTAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGAACCATAAGATTAACGCCGGTTTTAACCGGCGt                                                                                                                                              <  1:3100304/150‑1 (MQ=255)
         tgctgGGCGCAACCACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTTTAACCGGCGt                                                                                                                                              <  2:212320/150‑1 (MQ=255)
          gctgGGCGCAACCACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGtt                                                                                                                                             >  1:3874785/1‑150 (MQ=255)
           ctgGGCGCAACCACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTTTAACCGGCGTTa                                                                                                                                            <  2:2710806/150‑1 (MQ=255)
           ctgGGCGCAACCACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTTTAACCGGCGTTa                                                                                                                                            <  2:1523981/150‑1 (MQ=255)
            tgGGCGCAACCACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTaa                                                                                                                                           >  2:938798/1‑150 (MQ=255)
            tgGGCGCAACAACTAGTGAACAGCTTAAAAATAACACCGAAATTTTGAATCTGGATTTAAGCGAAGACTTATTAGCTTAAATTGAAGCGGTGCATCTGTTTTATACTTATCCGGCACAATAAGATTAACGCCGGTTAAAACCGGCGTTaa                                                                                                                                           <  1:1685543/150‑1 (MQ=255)
             gggCGCAACCACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTaaa                                                                                                                                          <  2:393371/150‑1 (MQ=255)
             gggCGCAACCACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTaaa                                                                                                                                          <  2:2004865/150‑1 (MQ=255)
                 gcAACCACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATAtt                                                                                                                                      >  1:100068/1‑150 (MQ=255)
                  cAACCACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTa                                                                                                                                     >  2:3340694/1‑150 (MQ=255)
                   aaCCACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTaa                                                                                                                                    >  2:808111/1‑150 (MQ=255)
                   aaCCACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTaa                                                                                                                                    >  1:295343/1‑150 (MQ=255)
                   aaCCACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTaa                                                                                                                                    >  1:2340143/1‑150 (MQ=255)
                    aCCACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGTTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAAt                                                                                                                                   <  1:3335595/150‑1 (MQ=255)
                        cGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACg                                                                                                                               <  2:1186132/150‑1 (MQ=255)
                         gctGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAACACGTATTAGCTTAAATGGAAGCGGTGCATAAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACgg                                                                                                                              <  1:3668644/148‑1 (MQ=255)
                         gATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACgg                                                                                                                              >  2:288968/1‑150 (MQ=255)
                                 aGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCa                                                                                                                      <  2:2442257/150‑1 (MQ=255)
                                     gAAAACTAACCTCGAACGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATc                                                                                                                  <  1:3007769/150‑1 (MQ=255)
                                     gAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATc                                                                                                                  <  2:4192667/150‑1 (MQ=255)
                                      aaaaCTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATcc                                                                                                                 >  1:3233743/1‑150 (MQ=255)
                                      aaaaCTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATcc                                                                                                                 >  1:3467983/1‑150 (MQ=255)
                                       aaaCTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCca                                                                                                                <  2:1633739/150‑1 (MQ=255)
                                        aaCTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCcac                                                                                                               >  1:63653/1‑150 (MQ=255)
                                          cTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAg                                                                                                             <  2:11486/150‑1 (MQ=255)
                                           tAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAgc                                                                                                            >  1:533799/1‑150 (MQ=255)
                                            aaCATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAgcg                                                                                                           <  1:1476553/150‑1 (MQ=255)
                                              cATCGCAAGTGTGCATCTGGAGATAAGCGAAGCCGTATTAGCTGAAAGTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATACGATTAACGCCGGTTAAAACCTGCGTTAAATATTAATGACGGCGCGGCCAGATCCACAGCGcc                                                                                                         <  2:1201367/150‑1 (MQ=255)
                                              cATCGAAAGTTTGCATCTGGAGTTGAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGcc                                                                                                         >  2:3170144/1‑150 (MQ=255)
                                              cATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGcc                                                                                                         >  2:3170265/1‑150 (MQ=255)
                                               aTCGAACGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCg                                                                                                        <  1:488418/150‑1 (MQ=255)
                                                tCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGt                                                                                                       >  2:1497636/1‑150 (MQ=255)
                                                    aaGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTAtt                                                                                                   <  1:391896/150‑1 (MQ=255)
                                                    aaGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTAtt                                                                                                   <  2:2992389/150‑1 (MQ=255)
                                                    aaGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTAtt                                                                                                   <  2:2810047/150‑1 (MQ=255)
                                                      gTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGc                                                                                                 <  1:1161228/150‑1 (MQ=255)
                                                      gTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGc                                                                                                 <  2:752635/150‑1 (MQ=255)
                                                          gCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAgc                                                                                             >  1:1232356/1‑150 (MQ=255)
                                                            aTCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAgcgc                                                                                           <  1:4153758/150‑1 (MQ=255)
                                                             tCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCa                                                                                          >  1:3063645/1‑150 (MQ=255)
                                                                    ttAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAgcg                                                                                   <  1:1260384/150‑1 (MQ=255)
                                                                      aaGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCa                                                                                 <  1:2050101/150‑1 (MQ=255)
                                                                      aaGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCa                                                                                 <  1:3210815/150‑1 (MQ=255)
                                                                       aGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCAc                                                                                >  1:3825842/1‑150 (MQ=255)
                                                                       aGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCAc                                                                                >  1:1312841/1‑150 (MQ=255)
                                                                        gCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATTACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCAcc                                                                               >  2:3099973/1‑150 (MQ=255)
                                                                        gCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCAcc                                                                               >  2:1797393/1‑150 (MQ=255)
                                                                         cGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCg                                                                              >  2:472871/1‑150 (MQ=255)
                                                                           aaGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGaa                                                                            <  1:951652/150‑1 (MQ=255)
                                                                           aaGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGaa                                                                            <  2:2327166/150‑1 (MQ=255)
                                                                             gACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAgc                                                                          <  2:3799621/150‑1 (MQ=255)
                                                                                gTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAgccaa                                                                       <  1:3375892/150‑1 (MQ=255)
                                                                                 tATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTACCGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAgccaat                                                                      <  2:1717907/150‑1 (MQ=255)
                                                                                       ccgAAATGGCAGCGGTGCATCAGGTATATACTTAGCCGGCACCATACGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAgccaatgccaat                                                                <  2:592704/148‑1 (MQ=255)
                                                                                       cTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAgccaatgccaat                                                                <  2:3874237/150‑1 (MQ=255)
                                                                                       cTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAgccaatgccaat                                                                <  2:166754/150‑1 (MQ=255)
                                                                                       cTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAgccaatgccaat                                                                >  1:3522538/1‑150 (MQ=255)
                                                                                        tGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGccaatgccaatg                                                               >  1:402494/1‑150 (MQ=255)
                                                                                        tGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGccaatgccaatg                                                               >  2:2635791/1‑150 (MQ=255)
                                                                                        tGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGccaatgccaatg                                                               >  1:2526437/1‑150 (MQ=255)
                                                                                        tGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGccaatgccaatg                                                               >  2:1656521/1‑150 (MQ=255)
                                                                                                     gTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGa                                                  >  2:1447623/1‑150 (MQ=255)
                                                                                                      tGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGAt                                                 >  2:2505666/1‑150 (MQ=255)
                                                                                                            aGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTAt                                           >  2:3557961/1‑150 (MQ=255)
                                                                                                             ggTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATc                                          <  2:1161354/150‑1 (MQ=255)
                                                                                                                 tataCTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTa                                      >  2:1855085/1‑150 (MQ=255)
                                                                                                                  ataCTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTAc                                     >  1:2230323/1‑150 (MQ=255)
                                                                                                                   taattATCCGCCACCATAACATTAACGCCGGATAAAACCGGCGTAAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACACCGCACCGAAGCCAATGCCAATGGGCACGACCGCGATGCCTATCATTAcc                                    <  2:2176936/147‑1 (MQ=255)
                                                                                                                   taCTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGAGCCACAGCGCCGTTATAGACAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTAcc                                    >  2:3028088/1‑150 (MQ=255)
                                                                                                                    aCTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCg                                   <  2:1985223/150‑1 (MQ=255)
                                                                                                                       tATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCa                                >  1:261689/1‑150 (MQ=255)
                                                                                                                           cGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGa                            >  2:3223694/1‑150 (MQ=255)
                                                                                                                             gCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGTACGACCGGGATGCCTATCATTACCGCCAGCGAGt                          <  2:3839959/150‑1 (MQ=255)
                                                                                                                             gCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATGAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCCCCGAAGCCAATGCCAATGGGCCCGCCCGGGATGCCTATCATTACCGCCCGCGAGt                          <  2:971284/150‑1 (MQ=255)
                                                                                                                               aCCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTATCGCCAGCGAGTaa                        <  2:1355226/150‑1 (MQ=255)
                                                                                                                                 ttaatatttAACGCCGGTTTTAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAAt                      >  2:3412806/8‑150 (MQ=255)
                                                                                                                                 cATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAAt                      <  2:2748432/150‑1 (MQ=255)
                                                                                                                                 cATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAAt                      <  2:1944277/150‑1 (MQ=255)
                                                                                                                                   tAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGc                    <  2:1753213/150‑1 (MQ=255)
                                                                                                                                       aTTACCGCCGGTTAAACCCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCCCCGCGCCGTTATTGCCAGCGCAAACCGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAg                <  1:4242504/150‑1 (MQ=255)
                                                                                                                                       aTTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAg                >  1:282223/1‑150 (MQ=255)
                                                                                                                                             gCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGTCAATGCCAATGGGCCCGACCGGGCTGCCTCTCATTACCGCCAGCGAGTCAATGCCCAGCCTcaa          >  2:3915996/1‑150 (MQ=255)
                                                                                                                                              ccGGTTAAAACCGGCGTTAAATATTAATGATGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCATcaac         >  2:3461370/1‑150 (MQ=255)
                                                                                                                                              ccGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCATcaac         >  2:3743939/1‑150 (MQ=255)
                                                                                                                                                 gTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCATCaacaac      <  1:2413587/150‑1 (MQ=255)
                                                                                                                                                    aaaaCCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCATCAACAACATg   <  2:2595584/150‑1 (MQ=255)
                                                                                                                                                    aaaaCCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCATCAACAACATg   <  1:1129955/150‑1 (MQ=255)
                                                                                                                                                     aaaCCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCATCAACAACATgg  >  2:1245467/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CCAGCACTCTGCTGGGCGCAACCACGATGGATCAGCTGAAAACTAACATCGAAAGTTTGCATCTGGAGTTAAGCGAAGACGTATTAGCTGAAATTGAAGCGGTGCATCAGGTTTATACTTATCCGGCACCATAAGATTAACGCCGGTTAAAACCGGCGTTAAATATTAATGACGGCGCTGCCAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCATCAACAACATGG  >  NC_000913/2972504‑2972802

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: