Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,046,050 T→G 7.9% intergenic (‑149/+118) ygfF ← / ← gcvP putative NAD(P)‑dependent oxidoreductase/glycine decarboxylase, PLP‑dependent, subunit P of glycine cleavage complex

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,046,0500TG 7.9% 495.8 / 10.2 213intergenic (‑149/+118)ygfF/gcvPputative NAD(P)‑dependent oxidoreductase/glycine decarboxylase, PLP‑dependent, subunit P of glycine cleavage complex
Reads supporting (aligned to +/- strand):  ref base T (73/117);  new base G (16/5);  total (89/124)
Fisher's exact test for biased strand distribution p-value = 1.78e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 219 total aligned reads displayed.

TTTGTTTTTCCGAGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGC  >  NC_000913/3045901‑3046199
                                                                                                                                                     |                                                                                                                                                     
tttGTTTTTCCGAGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGt                                                                                                                                                       >  1:311716/1‑150 (MQ=255)
    tttttCCGAGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGGTccc                                                                                                                                                   >  2:732776/1‑150 (MQ=255)
      tttCCGAGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCt                                                                                                                                                 <  2:912274/150‑1 (MQ=255)
      tttCCGAGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGGTccccg                                                                                                                                                 >  1:3674294/1‑149 (MQ=255)
      tttCCGAGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGGTCCCCt                                                                                                                                                 >  1:2012002/1‑150 (MQ=255)
          cGAGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGTCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCAcc                                                                                                                                             <  2:4157342/150‑1 (MQ=255)
          cGAGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGTCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCAcc                                                                                                                                             <  2:2987785/150‑1 (MQ=255)
           gagaAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGGTCCCCTCAccc                                                                                                                                            >  1:3604883/1‑150 (MQ=255)
            agaAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCt                                                                                                                                           >  1:1321278/1‑150 (MQ=255)
            agaAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTATTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTCAATATTCGCGCCAGTGCCGGCCTGGTCCCCGCAcccg                                                                                                                                           >  1:2916661/1‑149 (MQ=255)
                  attaattCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAAcccg                                                                                                                                     >  2:1714879/1‑149 (MQ=255)
                      attCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCACACCCAAACCCACTcc                                                                                                                                 >  2:969701/1‑150 (MQ=255)
                        tCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTcccc                                                                                                                               <  1:3157565/150‑1 (MQ=255)
                        tCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCGAACCCACTcccc                                                                                                                               >  1:2501962/1‑150 (MQ=255)
                         cGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCa                                                                                                                              <  2:2791326/150‑1 (MQ=255)
                         cGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCa                                                                                                                              <  2:2791463/150‑1 (MQ=255)
                          gTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCAATACCCaa                                                                                                                             >  1:1534855/1‑150 (MQ=255)
                          gTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCACACCCAAACCCACACCCCaa                                                                                                                             >  1:3981343/1‑150 (MQ=255)
                           ttGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCaaa                                                                                                                            <  2:2617629/150‑1 (MQ=255)
                                   tCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAg                                                                                                                    <  1:2267743/150‑1 (MQ=255)
                                   tCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAg                                                                                                                    <  1:4245395/150‑1 (MQ=255)
                                    cTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGTGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCGCCCCTTTGGGGAGAgg                                                                                                                   <  1:1122701/150‑1 (MQ=37)
                                    cTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCGCCCCTTTGGGGAGAgg                                                                                                                   <  1:3152753/150‑1 (MQ=37)
                                     tttGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCGCCCCTTTGGGGAGAggg                                                                                                                  <  2:3132913/150‑1 (MQ=37)
                                     tttGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCACTCCCCAAAGGGGCGAggg                                                                                                                  >  2:1793186/1‑150 (MQ=255)
                                     tttGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCACACCCCAAAGGGGCGAggg                                                                                                                  >  1:3542100/1‑150 (MQ=255)
                                     tttGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCGCCCCCAAACCCACACCCCCAAGGGGCGAggg                                                                                                                  >  1:1916922/1‑150 (MQ=255)
                                     tttGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGGTCCCCTCACCCTAACCCTCGCCCCTTTGGGGAGAggg                                                                                                                  <  2:3286918/150‑1 (MQ=255)
                                       tGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTCAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCCCCCAAACCCACTCCCCAAAGggggggggggg                                                                                                                >  2:1996476/1‑149 (MQ=39)
                                        gagaTTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAAACCTCTCCCCAAAGGGGCGAGGGGAc                                                                                                               >  2:311943/1‑150 (MQ=255)
                                           aTTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGt                                                                                                            <  1:60717/150‑1 (MQ=255)
                                            ttGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTACCCTAAACCAAAAAAAAAACACAAATGGGCGAGGGGACTGTc                                                                                                           >  2:949033/1‑150 (MQ=255)
                                             tGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCt                                                                                                          <  2:4074341/150‑1 (MQ=255)
                                              gCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTg                                                                                                         <  1:3762745/150‑1 (MQ=255)
                                               cTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGa                                                                                                        <  2:1371855/150‑1 (MQ=255)
                                                tAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTGCCCTCACCCGAAACCACTCCCCAAAGGGGCGAGGGGAGtgtgtggg                                                                                                       >  1:553008/1‑148 (MQ=37)
                                                tAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGGTCCCCGCACCCTAACCCTCTCCCCCAAGGGGCGGGGGGGCTGTCTGAg                                                                                                       >  2:1171026/1‑150 (MQ=255)
                                                 aaCAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCACACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGc                                                                                                      >  2:3542339/1‑150 (MQ=255)
                                                     aaCTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCACACCCAAACCCACACCCCCAAGGGGCGAGGGGACGGTCTGAGCACtt                                                                                                  >  1:325555/1‑150 (MQ=255)
                                                      aCTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCAAACCCACACCCCCAAGCGGCGGGGGGACAGTCTGAGCACtgg                                                                                                 >  2:895828/1‑148 (MQ=255)
                                                       cTGGCTCAATCTCCAATGTTGGCACGCTGTGCCCCCTCGCCCTTTCCGGGAGAGGGCCGGGGTGAGGGTAAAGATTCGCGCCAGTGCCGGCCTGGTCCCCTCACCCTACCCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACtttt                                                                                                <  2:3171296/150‑1 (MQ=25)
                                                              actCTTCAATGTTGGCACGCTGTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACttt                                                                                         <  2:485190/148‑1 (MQ=255)
                                                               aTCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCACTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTGTTGTACTTTg                                                                                        >  2:3898072/1‑150 (MQ=255)
                                                                 cTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTGAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTc                                                                                      <  2:1610063/150‑1 (MQ=255)
                                                                 cTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATCTTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTc                                                                                      <  2:1760052/150‑1 (MQ=255)
                                                                   tCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGTACTGTCTGAGCACTGTTGTACTTTGTCATc                                                                                   >  2:1581782/1‑150 (MQ=255)
                                                                   tCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCGCACCCAAACCCACACCCCAAAGGGGCGAGGGGCCGGTCTGAGCACTTTTGTACTTTGTCaa                                                                                    >  2:4097991/1‑149 (MQ=255)
                                                                    cAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTACATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCACTCCCCAAAGGGGCGGGGGGCCTGTCGGAGCACGGTTGTACGTTGTCATc                                                                                   >  1:1944965/1‑150 (MQ=25)
                                                                    cAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATc                                                                                   <  1:943909/150‑1 (MQ=255)
                                                                       tGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGTGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGTCTGTCTGAGCACTTTTGTACTTTGTCATCTGa                                                                                <  2:1257010/150‑1 (MQ=255)
                                                                       tGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGa                                                                                <  1:2457598/150‑1 (MQ=255)
                                                                        gTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGGTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGAc                                                                               <  1:246791/150‑1 (MQ=255)
                                                                           ggCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACGGTCTGAGCACTTTTGTACTTTGTCATCTGACtta                                                                            >  2:2933699/1‑148 (MQ=255)
                                                                            gCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGTCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACGGTCTGAGCACTTTTGTACTTTGTCATCTGACTaaa                                                                           >  2:2116430/1‑150 (MQ=255)
                                                                            gCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTaaa                                                                           <  2:1223655/150‑1 (MQ=255)
                                                                              aCGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTaaaaa                                                                         <  2:3669886/150‑1 (MQ=255)
                                                                              aCGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTaaaaa                                                                         <  2:2289631/150‑1 (MQ=255)
                                                                              aCGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTaaaaa                                                                         >  2:2961036/1‑150 (MQ=255)
                                                                              aCGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCGCACCCAAAACCACAACCCAAAGGGGCGCGGGGCCTGTCTGAGCACGTTTTTAGTGTGTCATATGGCTaaaaa                                                                         >  1:3442865/1‑150 (MQ=255)
                                                                              aCGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGGTGCCCACAACCAAACCCACAACCCCAAAGGGCGGGGGGCCGGTCGGGGCACATTTGTTATTTTTGATAAGACaaaaaa                                                                         >  1:18303/1‑150 (MQ=255)
                                                                               cGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAg                                                                        <  1:1665320/150‑1 (MQ=255)
                                                                               cGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAg                                                                        <  1:3082466/150‑1 (MQ=255)
                                                                                 cTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGgc                                                                      <  1:3040535/150‑1 (MQ=255)
                                                                                  ttttCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGgcg                                                                     <  2:170408/150‑1 (MQ=255)
                                                                                  ttttCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGgcg                                                                     <  2:170279/150‑1 (MQ=255)
                                                                                          cTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCGCACCCTAACCCACTACCCAAAGGGGCGAGGGGCCAGTCAGAGCACATTTGTACAGTGTCATCTGACTAAAAAGGCGCCGAAgcg                                                             >  2:2321882/1‑150 (MQ=255)
                                                                                           tCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTATCCCTCGCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAgcgc                                                            <  1:368106/150‑1 (MQ=255)
                                                                                           tCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCGCACCCAAACCCACTCCCCAAAGGGGCGCGGGGCAGGTAGGAGCACATTTGTTCTTTGTCAAATGACTAAAAAAGCGCCGAAacgc                                                            >  1:2385427/1‑150 (MQ=255)
                                                                                           tCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTACCCACACCCTAACCCCCAACCCAAAGGGGCGAGGGGCCTGTCTGAGCACATTTTTAATTTTTAATCTTAAGAAAAAGGCGCCCAAgccc                                                            >  2:819140/1‑148 (MQ=255)
                                                                                           tCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGGTCCCCTCACCCTAACCCACTCCCCCAAGGGGCGAGGGGACAGTCTGAGCACTTTTGTACTTTGTCATATGACTAAAAAGGCGCCGAAgcgc                                                            >  2:3767486/1‑150 (MQ=255)
                                                                                           tCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGGAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTACCCCCAACCCCAAAGGGCCGCGGGGCCTGCCGGGCCCGGTTTGGACGTTGCCAACTGACAAAAAAGCCCCCGaccccc                                                            >  2:2458441/1‑145 (MQ=12)
                                                                                             gCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTACCCGCACCCAAACCCACACCCCCAAGGGGCGAGGGGACCGTCTGAGCACATTTGTACTTTGTCATCTGACTAAAAATGCGCCCAAGCGccg                                                          >  2:1520636/1‑149 (MQ=255)
                                                                                               ccTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCTTCGGCGCCttt                                                        <  1:1734972/150‑1 (MQ=255)
                                                                                               ccTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCTTCGGCGCCttt                                                        <  1:2654666/150‑1 (MQ=255)
                                                                                               ccTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGCTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCTTCGGCGCCttt                                                        <  1:2544908/150‑1 (MQ=37)
                                                                                                cTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTa                                                       <  2:2369750/150‑1 (MQ=255)
                                                                                                cTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTa                                                       <  2:2693472/150‑1 (MQ=255)
                                                                                                  ttCAGGGAGAGGGCCGGGGTGAGGGTAAATCTTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGa                                                     <  2:252559/150‑1 (MQ=255)
                                                                                                   tccgggAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGaa                                                    <  2:314952/147‑1 (MQ=255)
                                                                                                    cAGGGAGAGGGCCGGGGTGAGGGAAAATATACGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGaaa                                                   <  2:1562640/150‑1 (MQ=255)
                                                                                                       ggAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATa                                                <  1:969701/150‑1 (MQ=255)
                                                                                                       ggAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTCAGAAAATa                                                <  2:3736513/150‑1 (MQ=255)
                                                                                                       ggAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGGTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATa                                                <  1:2702931/150‑1 (MQ=255)
                                                                                                         agagGGCCGGGGTGGGGGTAAATATTCGCGCCAGTGCCGGCCTGTTGCCCTAACCCTAAACCTCTACCCAAAGGGGGGGGGGGGGTGTGTGAGGACGTTTTTTATTTGTGATATGTATTAAAATGCGCCGAAGCGCCGTTATAAAATatt                                              >  1:3412290/1‑148 (MQ=255)
                                                                                                                cGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCa                                       <  1:213602/150‑1 (MQ=255)
                                                                                                                cGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCa                                       <  1:802933/150‑1 (MQ=255)
                                                                                                                cGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCa                                       <  1:4080449/150‑1 (MQ=255)
                                                                                                                cGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCGCACCCAAACCCTCTCCCCAAAGGGGCGAGGGGACAGTCTGAGCACTTTTTTAATTTTTCATCTGAAAAAAAAGGCGCCCAAGCCCCGTTAGAAAAAAGTCGaaaaa                                       >  2:4012037/1‑147 (MQ=25)
                                                                                                                  gggTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGt                                     <  2:2660781/150‑1 (MQ=255)
                                                                                                                     tGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGaa                                  <  2:1321278/150‑1 (MQ=255)
                                                                                                                          gTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCAAACCCTCTACCCAAAGGGGCGAGGGGACTGTCTGAGCACATTTTTACTTTGTCATCTTAATAAAAAGGCGCCGAAGCGCCTTTATAAAATAGTAGAATCATTGAATTACt                             >  2:94177/1‑150 (MQ=255)
                                                                                                                            accgcttCGCGCCAGTGCCGGCCGGTGCCCCTCCCCCGACCCCTCGCCCCCCCGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGGCATCTGACTAAAACGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTgg                           <  2:3381340/145‑1 (MQ=255)
                                                                                                                              atatTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTa                         <  2:325555/150‑1 (MQ=255)
                                                                                                                                  tCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCg                     <  2:148899/150‑1 (MQ=255)
                                                                                                                                   cgcgCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGc                    <  1:2974673/150‑1 (MQ=255)
                                                                                                                                       ccAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAAt                <  2:4134153/150‑1 (MQ=255)
                                                                                                                                        cAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATc               <  1:2348347/150‑1 (MQ=255)
                                                                                                                                        cAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATc               <  1:1083138/150‑1 (MQ=255)
                                                                                                                                              cGGCCTGTTACCCTCACCCTAACCCTCAACCCAAAGGGGCGAGGGGACAGTCTGAGCACTTTTGTACTTTGTCATATGACTAAAAAGGCGCCCAAGCGCCTTTAGAAAAAAGTAGAATAAGTGAAATAATGGTATTAGCTAATCGGTACg         >  2:2466567/1‑150 (MQ=255)
                                                                                                                                                     ttCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGGGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGc  >  2:3089768/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TTTGTTTTTCCGAGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAGC  >  NC_000913/3045901‑3046199

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: