Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 238,507 G→T 8.0% S77* (TCA→TAA)  ykfM ← lethality reduction protein, putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913238,5070GT 8.0% 524.6 / 5.0 170S77* (TCA→TAA) ykfMlethality reduction protein, putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (81/70);  new base T (6/13);  total (87/83)
Fisher's exact test for biased strand distribution p-value = 8.91e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 171 total aligned reads displayed.

GCAGGGGTGATAATTAGTATTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAA  >  NC_000913/238359‑238656
                                                                                                                                                    |                                                                                                                                                     
  aGGGGTGATAATTAGTATTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGAtt                                                                                                                                                    <  2:142704/150‑1 (MQ=255)
  aGGGGTGATAATTAGTATTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGAtt                                                                                                                                                    <  1:881742/150‑1 (MQ=255)
  aGGGGTGATAATTAGTATTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGAtt                                                                                                                                                    <  2:2041757/150‑1 (MQ=255)
  aGGGGTGATAATTAGTATTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGAtt                                                                                                                                                    <  2:142543/150‑1 (MQ=255)
     ggTTATAATTAGTATTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTtta                                                                                                                                                 <  2:2613978/150‑1 (MQ=255)
     ggTGATAATTAGTATTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTtta                                                                                                                                                 >  1:3959259/1‑150 (MQ=255)
     ggTGATAATTAGTATTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTtta                                                                                                                                                 >  1:2909938/1‑150 (MQ=255)
        gATAATTAGTATTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTttatta                                                                                                                                              >  2:16591/1‑150 (MQ=255)
        gATAATTAGTATTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTttatta                                                                                                                                              >  2:3166630/1‑150 (MQ=255)
         aTAATTAGTATTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTtattat                                                                                                                                             >  1:2407129/1‑150 (MQ=255)
         aTAATTAGTATTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTtattat                                                                                                                                             >  1:253717/1‑150 (MQ=255)
          tAATTAGTATTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATc                                                                                                                                            <  2:3863177/150‑1 (MQ=255)
          tAATTAGTATTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATc                                                                                                                                            <  2:3808474/150‑1 (MQ=255)
          tAATTAGTATTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATc                                                                                                                                            <  2:734405/150‑1 (MQ=255)
              tAGTATTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCgtaa                                                                                                                                        <  2:1974467/150‑1 (MQ=255)
              tAGTATTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCgtaa                                                                                                                                        <  1:2805140/150‑1 (MQ=255)
              tAGTATTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCgtaa                                                                                                                                        <  1:3976213/150‑1 (MQ=255)
              tAGTATTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCgtaa                                                                                                                                        <  1:2891491/150‑1 (MQ=255)
                 tATTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCgtaagta                                                                                                                                     >  2:1336489/1‑150 (MQ=255)
                 tATTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCgtaagta                                                                                                                                     >  1:2004348/1‑150 (MQ=255)
                 tATTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCgtaagta                                                                                                                                     <  2:3146630/150‑1 (MQ=255)
                   ttGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTaaa                                                                                                                                   <  2:2064089/150‑1 (MQ=255)
                   ttGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTaaa                                                                                                                                   <  2:1991020/150‑1 (MQ=255)
                    tGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTaaaa                                                                                                                                  <  2:2478347/150‑1 (MQ=255)
                     gACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAAt                                                                                                                                 <  1:3290202/150‑1 (MQ=255)
                     gACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAAt                                                                                                                                 <  1:595143/150‑1 (MQ=255)
                      aCATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAAtt                                                                                                                                >  2:4223647/1‑150 (MQ=255)
                         tatCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACa                                                                                                                             <  2:1038476/150‑1 (MQ=255)
                          atCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACAt                                                                                                                            >  1:3521414/1‑150 (MQ=255)
                           tCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGGTAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATg                                                                                                                           <  1:1971388/150‑1 (MQ=255)
                           tCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATg                                                                                                                           >  1:73411/1‑150 (MQ=255)
                            ccTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGa                                                                                                                          >  1:1293262/1‑150 (MQ=255)
                             cTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGaa                                                                                                                         <  2:752386/150‑1 (MQ=255)
                             cTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGaa                                                                                                                         >  1:4184270/1‑150 (MQ=255)
                              tgtgACACAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAat                                                                                                                        <  2:2150254/150‑1 (MQ=255)
                              tgtgACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAat                                                                                                                        >  1:1739121/1‑150 (MQ=255)
                              tgtgACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAat                                                                                                                        >  1:569402/1‑150 (MQ=255)
                              tgtgACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAat                                                                                                                        >  1:2491256/1‑150 (MQ=255)
                              tgtgACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAat                                                                                                                        >  2:1064935/1‑150 (MQ=255)
                              tgtgACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAat                                                                                                                        >  2:4074657/1‑150 (MQ=255)
                              tgtgACAAAAGGAGCTATTAAAGGAGCTATTACGATAGCTATTAGAAAAAATATAAGAGTTAGCTGTATTGATATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAat                                                                                                                        <  2:1779581/150‑1 (MQ=255)
                                    aaaaGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTaa                                                                                                                  <  2:1885873/150‑1 (MQ=255)
                                    aaaaGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTaa                                                                                                                  <  2:3536924/150‑1 (MQ=255)
                                    aaaaGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTaa                                                                                                                  >  1:2113583/1‑150 (MQ=255)
                                    aaaaGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTaa                                                                                                                  >  1:1310674/1‑150 (MQ=255)
                                     aaaGGAGCTAATACAGGAGCTATTCCGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTaaa                                                                                                                 <  1:2220337/150‑1 (MQ=255)
                                       aGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTaaaaa                                                                                                               <  1:2576479/150‑1 (MQ=255)
                                       aGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGGATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTaaaaa                                                                                                               <  2:10444/150‑1 (MQ=255)
                                          aGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGa                                                                                                            <  2:2783540/150‑1 (MQ=255)
                                          aGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGa                                                                                                            <  2:2783559/150‑1 (MQ=255)
                                                          aTTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCg                                                                                            >  1:3157637/1‑150 (MQ=255)
                                                          aTTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCg                                                                                            >  1:1023377/1‑150 (MQ=255)
                                                           ttACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGa                                                                                           >  1:2805201/1‑150 (MQ=255)
                                                               gATAGCTATTATTAAAAATATAAGAGTTAGCTTTATTGTTATGTCTGTTGCGAAATTGAATACCTTCGATTTATTTATAAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGca                                                                                       <  2:2899958/150‑1 (MQ=255)
                                                                aTAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGcac                                                                                      >  1:2269976/1‑150 (MQ=255)
                                                                aTAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAAAGACATGAAACCGAAGcac                                                                                      >  1:1706717/1‑150 (MQ=255)
                                                                   gCTATTAGTAAAAATATAAGAGTTAGCTGTATTTTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTTATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGa                                                                                   <  2:2094722/150‑1 (MQ=255)
                                                                      attattAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATc                                                                                <  2:3105158/150‑1 (MQ=255)
                                                                         tttAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTTACTACCTTCGTTTTTTTTATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAAc                                                                             <  2:3861232/148‑1 (MQ=255)
                                                                           tAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATAGTAAGTAAAAATAAAAGAATATTTAAAAATGAAAAAGACAAGAAACCGAAGCACAGAAacaacaa                                                                           >  1:1120568/1‑149 (MQ=255)
                                                                               aaTATATGCTTTCGCTTTTTTGTTATGTCTGTGGCTAAATTTACTACCTTCTTTTTTTTGATTAAGATTTTTTTTATTATCTTAATTAAAATTAAATCAATATTAAAAATGGAAAACGACATGAAACCGAAGCACAGAATCAACATtctc                                                                       <  1:3993645/150‑1 (MQ=255)
                                                                               aaTATACGTGTTAGCTTTTTTTTTTTGTCTGTGGCTAATTTGACTACCTTCTTTTTTTTTATTAAGAATTATTTTATTATCTTAATTAAAATTAATTTAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATtctc                                                                       <  1:304007/150‑1 (MQ=255)
                                                                               aaTATAAGTTTTAGTTTTTTTTTTTTGTCTGTGGCTAATTTGACTACCTTCTTTTTTTTGATTAATATTTATTTTATTTTCGTAATTAATATTACATTATTTTTTAAAATGTAAAACGACATGAAACCGAAGCACAGAATCAACATtctc                                                                       <  1:3592037/150‑1 (MQ=255)
                                                                                    aaGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACAATCTCCAATc                                                                  >  2:1492593/1‑150 (MQ=255)
                                                                                      gagTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATTAAACCGAAGCACAGAATCAACATTCTCCAATCAt                                                                >  2:1162358/1‑150 (MQ=255)
                                                                                        gTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTAAATGAATATATAAAAAGGGAAAAGACATGAAAACGAAGCACAGAAACAAAATTCTCCCAtaaaaa                                                              >  1:3597443/1‑145 (MQ=255)
                                                                                              tGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATAtt                                                        >  2:894164/1‑150 (MQ=255)
                                                                                                tATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGTTTTAGAATTATTTTATTATCGGAAGTAAAAATAAATTTATATTTAAAAAGGAAAAAGAAAAGAAAACGAAGCACAGAATCAACAATATCCAAACAAAAAAAAATTc                                                      >  1:1454/1‑150 (MQ=255)
                                                                                                  ttGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCg                                                    >  2:2675596/1‑150 (MQ=255)
                                                                                                   tGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTTTTTTATAAATATTTTATTATAGTAAGTAAAATTAAATGTATATTTTAAAAGGAAAAACACATTAAAACGAATCAAAGAATCAAAAATATACAAAAAAAAAATAtttaagt                                                   >  1:28537/1‑146 (MQ=255)
                                                                                                     ttATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTTATTAATAATGATTTTATTATAGTAAGTAAAAATAAAATAATATTTAAAAAGGAAAACGACAAGAAACCGAAGCAAAGAAAAAACAATCTCCAATAATAAAATATTTACGTgg                                                 >  1:1775892/1‑150 (MQ=255)
                                                                                                      tttGTCTGTGTCTAATTTGACTACCTTCGTTTTTTTGATTAAGAATTTTTTTATTATCGTAAGTAAAATTAAATTAATATTAAAAATGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGa                                                <  1:1499853/148‑1 (MQ=255)
                                                                                                       aTGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAg                                               >  2:3755653/1‑150 (MQ=255)
                                                                                                       aTGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAg                                               >  2:592211/1‑150 (MQ=255)
                                                                                                       aTGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATAGTTAGTAAAAATAAAATAATATTTAAAAAGGAAAAAGACATGAAAACGAAACACAGAATCAAAAATCTACAAAAATAAAATATTTACGTGGAg                                               >  1:3740950/1‑150 (MQ=255)
                                                                                                        tGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAAAGATTTTATTAACCGAAGTAAAAATACAAGAAAATTTAAAAAAGAAAACCAAAAGAAACCCAAGCACACAAACAACAAAAACCAAACAAAAAATAATTCCGTGGAGc                                              >  1:2843525/1‑150 (MQ=255)
                                                                                                         gTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCa                                             >  2:387489/1‑150 (MQ=255)
                                                                                                                gctaaaTTGACTACCTTCTTTTTTTTGATTAAGAATGATTTTATTATCTTAAGTAAATTTACATGAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTttatt                                      <  2:1870563/147‑1 (MQ=255)
                                                                                                                gCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTttatt                                      <  2:1293262/150‑1 (MQ=255)
                                                                                                                gCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTttatt                                      >  2:497450/1‑150 (MQ=255)
                                                                                                                gCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTttatt                                      >  2:260590/1‑150 (MQ=255)
                                                                                                                gCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTttatt                                      >  2:1144709/1‑150 (MQ=255)
                                                                                                                gCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTttatt                                      >  2:2352393/1‑150 (MQ=255)
                                                                                                                  gAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGACGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCAGTGtattat                                    >  2:3385457/1‑150 (MQ=255)
                                                                                                                   aaaTTTACTACCTTCGTTTTTTTGATTAATAATTATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTattatt                                   <  2:4078208/150‑1 (MQ=255)
                                                                                                                   aaaTTGACTACCTCCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTattatt                                   <  1:2801569/150‑1 (MQ=255)
                                                                                                                      tttaCTACCTTCTTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGaa                                <  1:2787134/147‑1 (MQ=255)
                                                                                                                      ttGACTACCTTCGTTTTTTTGATTAATAATTATTTTATTATCGTAAGTAAAATTAAATGAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGaa                                <  2:602765/150‑1 (MQ=255)
                                                                                                                             ccTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATagag                         <  2:619815/150‑1 (MQ=255)
                                                                                                                                   tttttttGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTaa                   <  1:3322359/150‑1 (MQ=255)
                                                                                                                                        ttGATTAAGAATTATTTTATTATCGTAATTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCg              <  1:2163193/150‑1 (MQ=255)
                                                                                                                                        ttGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCg              >  2:657445/1‑150 (MQ=255)
                                                                                                                                           aTTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTa           >  1:3063108/1‑150 (MQ=255)
                                                                                                                                            ttAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaa          >  1:1890194/1‑150 (MQ=255)
                                                                                                                                              aatattGATTTTATTATCTTAATTAAAATTAATTTAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaaaa        <  1:1476322/145‑1 (MQ=255)
                                                                                                                                              aataaTTATTTTATTATCTTAATTAAAATTACATGAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaaaa        <  1:1571670/147‑1 (MQ=255)
                                                                                                                                                taaTTATTTTATTATCGTAATTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaaaaaa      <  2:359673/149‑1 (MQ=255)
                                                                                                                                                taaTTATTTTATTATCGTAATTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaaaaaa      <  2:2177079/149‑1 (MQ=255)
                                                                                                                                                taaTTATTTTATTATCGTAAGTAAATTTAAATGAATATTTAAAATGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaaaaaa      <  1:1328247/149‑1 (MQ=255)
                                                                                                                                                 aaTGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAc     >  2:2076099/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GCAGGGGTGATAATTAGTATTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAA  >  NC_000913/238359‑238656

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: