Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,270,143 C→T 7.8% intergenic (+541/+73) yhaC → / ← rnpB pentapetide repeats‑related protein/RNase P, M1 RNA component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,270,1430CT 7.8% 512.3 / 23.7 194intergenic (+541/+73)yhaC/rnpBpentapetide repeats‑related protein/RNase P, M1 RNA component
Reads supporting (aligned to +/- strand):  ref base C (98/77);  new base T (3/12);  total (103/91)
Fisher's exact test for biased strand distribution p-value = 1.28e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 199 total aligned reads displayed.

CTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAG  >  NC_000913/3269998‑3270292
                                                                                                                                                 |                                                                                                                                                     
   aCCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAAATGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTgg                                                                                                                                                >  2:3443539/1‑150 (MQ=37)
    ccGATACGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAACAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGa                                                                                                                                               <  2:2674181/150‑1 (MQ=255)
    ccGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGa                                                                                                                                               <  2:4019883/150‑1 (MQ=37)
    ccGATAAGCCGCATTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGa                                                                                                                                               <  1:1331939/150‑1 (MQ=37)
      gATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACa                                                                                                                                             <  2:1240962/150‑1 (MQ=37)
      gATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACa                                                                                                                                             <  2:1523340/150‑1 (MQ=37)
             cGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGtcattc                                                                                                                                      <  1:1308446/150‑1 (MQ=37)
               ctttctttTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAACGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGtcattcat                                                                                                                                    <  1:4138692/150‑1 (MQ=37)
                tttctttTGGGTATAGTGCCGTGGACAGTCATTCATCTTTCTGCCCCTCCACCAGCAAAAACCCGCCGAAGCGGGTTTCTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATc                                                                                                                                   <  1:4260236/150‑1 (MQ=255)
                 ttctttTGGGTATAGTGTCGTGGACAGTCATTAATATTTATGCCCCACCAAAAGCAAAAAACCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTTACCGATAAGCCGCTGTCTTTTGGGTATAGCGTCGTGGGCAGgcagtcatat                                                                                                                                  >  1:1452899/1‑148 (MQ=11)
                   ctttTGGGTATAGTGTCGTGGACAGTCTTTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCttt                                                                                                                                <  1:2523823/150‑1 (MQ=37)
                      ttGGGTATAGTGTCGTGGTCAGTCATTCATCTTTCTGCCCCTCCAAATGCAAAAACCCGCCGAAGCGGGTTTTTACGTAATCCAGGTGAAACTGAGCGTTAAGCCGCTTTGTTTTGGGTATTGCGTGGTGGAGAGTCATTGATGTTtgtg                                                                                                                             >  2:988120/1‑150 (MQ=11)
                      ttGGGTATAGTGTCGTGGACAGTCATTCATCTTTCAGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGACGTGGCCAGTCCATCAACTTTATg                                                                                                                             >  2:3750769/1‑150 (MQ=11)
                         ggTATAGTGTCGTGGACAGTCATTCATATTTATTCCCCACCAAAAGCAAAAACCCGCCCAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCGTTCTTTTGGGTATTGCGTCGTGGTCAGTAAATAATCTTTCTTccc                                                                                                                          >  1:2885007/1‑150 (MQ=11)
                               gtgtCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCaa                                                                                                                    <  2:4219032/150‑1 (MQ=37)
                                 gtCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCaaaa                                                                                                                  <  1:1818636/150‑1 (MQ=37)
                                  tCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCAGTCATATTTCAGCCCCTCCAAAAg                                                                                                                 >  1:937479/1‑150 (MQ=255)
                                     tGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTGTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCaa                                                                                                              >  1:3016545/1‑150 (MQ=37)
                                        aCAGTCATTCAACCTTCTGCCCCTCCAAAACAACAATCCCGCCGTATCTGTTTTTTACGTAAACCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCaaaaa                                                                                                           <  2:2725503/150‑1 (MQ=11)
                                            tcattcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACccg                                                                                                       >  1:976111/1‑150 (MQ=37)
                                               ttcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCAGTCATTCATCGGTCTGCCCCTCCAAAAGCAAAAACccgccg                                                                                                    >  2:3288336/1‑150 (MQ=255)
                                               ttcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACccgccg                                                                                                    >  1:2455983/1‑150 (MQ=37)
                                                 catCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGaa                                                                                                  >  2:95646/1‑150 (MQ=37)
                                                 catCTGTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAACCTGACCGATAAGCCGCTTTCTTTTGGGTCTAGCGACGAGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCCAAAACCCGCCGaa                                                                                                  >  2:246174/1‑150 (MQ=11)
                                                  atCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTAGAGCGACGTGGACAGTCATTCATAATTCAGCCCCTCCAAAAGCAAAAACCCGCCGAAg                                                                                                 >  2:3894475/1‑150 (MQ=11)
                                                   tCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGc                                                                                                <  1:3443539/150‑1 (MQ=37)
                                                   tCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTGTGGGGTATAGCGTCGTGGACAGGCAGTCATCGTTCTGCCCCTCCAAAAGCAAAAACCCGCCGCAGc                                                                                                >  2:3890088/1‑150 (MQ=11)
                                                    cTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCg                                                                                               <  2:2785288/150‑1 (MQ=37)
                                                     tttCTGCCACTCAAAAAGCAAAAACCCGCCGTAGCGGTTTTTTACGTAAACCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCgg                                                                                              <  1:3164099/150‑1 (MQ=11)
                                                         tGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTACACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCTTCGGCGGGttt                                                                                          <  2:1220890/150‑1 (MQ=11)
                                                         tGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTATGCCCCTCCAAAAGCAAAAACCCGCCGCAGCGGGttt                                                                                          >  2:4020339/1‑150 (MQ=255)
                                                          ggcccTCCACAAGGTGAAACCCGCCGGAGCGGGTTTTTACGTACATCAGGTGAAACTGACCGATACGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAACAGCAAAAACCCGCCGAAGCGGGtttt                                                                                         <  1:2506254/148‑1 (MQ=11)
                                                          gCCCCTCCAAAAGCACAAACCCGCCGCAGCGGTTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGtttt                                                                                         <  2:658847/150‑1 (MQ=255)
                                                          cccccTCCAAAAGCAAAAACCCGCCGAACCGGGTTTTTACGTAAACCACCTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGACATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGtttt                                                                                         <  2:563062/149‑1 (MQ=255)
                                                           ccccTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCAATCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGttttt                                                                                        >  1:2400413/1‑150 (MQ=37)
                                                             ccTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCGCTCCCAAAGCAAAAACCCGCCGCAGCGGGTTTTTAc                                                                                      >  2:2337941/1‑150 (MQ=255)
                                                              cTCCAAAAGCAAAAACCCGCCGAAGCTGGTTTTTACGTAAACCAGGTGTAACTTACCGATAAGCCGCTTTATTTTGGGGCTAGCGTCGTGGCCCCTACTTCAACGGTCTGGCCCGACCAAAGCAAAAACCCGCCGAATCGGGTGTTTaag                                                                                     >  2:3443922/1‑148 (MQ=12)
                                                               tCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTGTTTACGt                                                                                    >  1:3575107/1‑150 (MQ=37)
                                                                 cAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCAGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTaa                                                                                  >  2:255556/1‑150 (MQ=37)
                                                                  aaaaGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGCAGCGGGTTTTTACGTaaa                                                                                 >  2:893030/1‑150 (MQ=37)
                                                                   aaaGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGGTTGTACGTAAAt                                                                                >  2:1680974/1‑150 (MQ=255)
                                                                   aaaGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTATGCCCCTCCAAAAGCAAAAACCCCCCGAAGCGGGTTTTTACGTaaaa                                                                                >  1:1990221/1‑149 (MQ=255)
                                                                       cAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAgg                                                                            >  2:3594897/1‑150 (MQ=38)
                                                                       cAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTGTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAgg                                                                            >  1:443442/1‑150 (MQ=38)
                                                                        aaaaaCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGt                                                                           <  2:1391148/150‑1 (MQ=37)
                                                                        aaaaaCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGAAAGTCAATCATATTTCTGCCCCGCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGt                                                                           >  2:366356/1‑150 (MQ=255)
                                                                         aaaaCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTAAGTAAATCAGGTg                                                                          >  1:3244188/1‑150 (MQ=37)
                                                                          aaaCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATAAGGTGa                                                                         >  1:1100813/1‑150 (MQ=37)
                                                                            aCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGaaa                                                                       <  1:2934686/150‑1 (MQ=37)
                                                                            aCCCGCCGAACCCGGATTTTACGTACAACAGGTTAAACTGACCGATAAGCCGCTATCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTATTACGTAAATCAGGTGaaa                                                                       <  2:3224922/150‑1 (MQ=11)
                                                                              ccgccgAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCGCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACt                                                                     >  2:832364/1‑150 (MQ=37)
                                                                              ccgccgAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTATGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACt                                                                     >  1:1626964/1‑150 (MQ=37)
                                                                               cgccgAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTg                                                                    >  2:4058593/1‑150 (MQ=17)
                                                                                gccgAAGCGGGTTTTTACGTACACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGa                                                                   <  2:3499835/150‑1 (MQ=37)
                                                                                gccgAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTAAGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGa                                                                   >  2:1139766/1‑150 (MQ=255)
                                                                                  cgtatcGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCACCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGAcc                                                                 <  1:887819/145‑1 (MQ=255)
                                                                                   gAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCg                                                                >  1:2759444/1‑150 (MQ=37)
                                                                                     aGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGAt                                                              <  1:597830/150‑1 (MQ=37)
                                                                                       cGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATaa                                                            <  2:2145021/150‑1 (MQ=37)
                                                                                           ttattACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTAGAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCCGGTGAAACTGACCGATAAGCCg                                                        <  1:3827968/147‑1 (MQ=255)
                                                                                             tttACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCggg                                                      >  2:215302/1‑150 (MQ=38)
                                                                                                aCGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTc                                                   >  1:2641610/1‑150 (MQ=40)
                                                                                                    acatcAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTc                                               <  2:1922476/146‑1 (MQ=255)
                                                                                                    acatcAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTc                                               <  1:3966364/146‑1 (MQ=255)
                                                                                                    acatcAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTc                                               <  1:1053923/146‑1 (MQ=255)
                                                                                                    acaCCCGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTc                                               <  1:4109211/148‑1 (MQ=255)
                                                                                                      aCCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGt                                             >  2:2013024/1‑150 (MQ=255)
                                                                                                       ccAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTg                                            <  1:654992/150‑1 (MQ=255)
                                                                                                       ccACGTGAATCTGACCGATACGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTg                                            <  2:3075601/150‑1 (MQ=255)
                                                                                                         aGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACCCTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGa                                          >  2:1444520/1‑150 (MQ=255)
                                                                                                         aGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGa                                          <  2:2584954/150‑1 (MQ=255)
                                                                                                          ggTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCGCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGGGGaa                                         >  2:1558695/1‑149 (MQ=11)
                                                                                                           gtcaaaCTGACCGATAAGCCGCGTTCTTTTGGGTAGAGTGTCGTGGACAGTCATTCATCTGTCTGCCCCTCCACCCGCAAAAACCCGCCGAAGCGGGTTTTTACGTAACTCCGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACa                                        <  1:1951847/147‑1 (MQ=14)
                                                                                                           gTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATAATTATGCCCCAACAAAAACAAAAAACCGCCGAAGCCGGGTTTTAAGTAAATCAGGTGACACTGACCGATAAGCCGGGTGCTGTGGTGGaaa                                        >  2:1463191/1‑148 (MQ=11)
                                                                                                           gTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACCGTCATTCATCTTTCTGCCCCACCAAAAGCAAAAAACCGCCGCAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGCTAAGCCGGGGTCTGTCGTGGACa                                        >  1:3245325/1‑150 (MQ=14)
                                                                                                           gTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACa                                        <  1:2232762/150‑1 (MQ=255)
                                                                                                            tGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAg                                       >  2:299672/1‑150 (MQ=255)
                                                                                                              aaaCTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGtc                                     <  1:3891260/150‑1 (MQ=255)
                                                                                                               aaCTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGtca                                    >  2:3390598/1‑150 (MQ=255)
                                                                                                                 cTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCGTTATGCCCCGCCAAAAGCAAAAAACCCCCCAAGCGGGTTTTTTCGTAAAAAAGGGGAAACAGACCGATAAGCCGGGGTCTGGGGTGGGCAGtcatt                                  >  1:3653444/1‑150 (MQ=14)
                                                                                                                  tGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGGCAGTCATTAAAAATTATGCCCCGCCCAAAGCAAAAACCCCCCCAAGCGGGTTTTTTAGTAAATAAGGTGTAAAATACCGATAAGCCGGGTTCTGTGGGGGGCAGtcatta                                 >  1:1910131/1‑149 (MQ=9)
                                                                                                                  tGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGtcattc                                 >  2:696270/1‑150 (MQ=255)
                                                                                                                  tGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGtcattc                                 >  2:2208454/1‑150 (MQ=255)
                                                                                                                  tGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCAATAATATTTATGCCCCACCAAAAGCAAAAAACCCCCGAAGCGGGTTTTTAAGTAAAAAAGGTGAAACTGACCGATAAGCCGGGTTCTGTAGTGGACAGtcatta                                 >  1:3373189/1‑149 (MQ=14)
                                                                                                                     ccGATCCGCCGCGTTCTGTGGGGTATCGCGTCGTGGCCTGTCATTCATCTTTCTGCCCCTCCAACCGCAACCTGCCGCCGAAGCGGGTTTTTTCGTAAATCCGGTGAAACGGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATc                              <  1:3447407/150‑1 (MQ=17)
                                                                                                                     ccGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAATCCGGGTTCTGTCGTGGACAGTCATTCATc                              <  2:3712143/150‑1 (MQ=255)
                                                                                                                        atcaGCCGCTTTCTTTTGGGTATAGAGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAg                           <  1:2167845/147‑1 (MQ=255)
                                                                                                                            gCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCa                       <  2:3194867/150‑1 (MQ=255)
                                                                                                                            gCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCa                       <  2:3016545/150‑1 (MQ=255)
                                                                                                                            gCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCa                       <  1:4058593/150‑1 (MQ=255)
                                                                                                                            gCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCa                       <  1:177034/150‑1 (MQ=255)
                                                                                                                            gCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAAACCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCa                       >  2:1313421/1‑150 (MQ=255)
                                                                                                                             ccGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATAAGGTGAAACTGACCGATAATCCGGGTTCAGTCGTGGACAGTCAATCATCTAGGCCAg                      >  2:1929721/1‑150 (MQ=255)
                                                                                                                                   tctttTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCg                <  1:152922/150‑1 (MQ=255)
                                                                                                                                     ttttGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCACAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCt              <  2:2922880/150‑1 (MQ=255)
                                                                                                                                     ttttGGGTATAGCGTCGTGGACACTCATTCATCATTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCCATAAGCCCGGGTCTGTCGTGGACAGTCAATCAACTAGGCCAGCCAACGcg              >  1:1413326/1‑149 (MQ=17)
                                                                                                                                      tttGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCCATCGCTc             >  2:4192314/1‑150 (MQ=255)
                                                                                                                                      tttGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTc             <  1:792441/150‑1 (MQ=255)
                                                                                                                                                gCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCaa   <  1:3531088/150‑1 (MQ=255)
                                                                                                                                                gCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTAATCTAGGCCAGCAATCGCTCACTGGCTCaa   >  2:452097/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                                     
CTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAG  >  NC_000913/3269998‑3270292

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: