Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,608,695 C→G 14.1% intergenic (+46/+56) zntA → / ← tusA zinc, cobalt and lead efflux system/mnm(5)‑s(2)U34‑tRNA 2‑thiolation sulfurtransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,608,6950CG14.1% 660.4 / 33.5 224intergenic (+46/+56)zntA/tusAzinc, cobalt and lead efflux system/mnm(5)‑s(2)U34‑tRNA 2‑thiolation sulfurtransferase
Reads supporting (aligned to +/- strand):  ref base C (115/73);  new base G (5/28);  total (121/103)
Fisher's exact test for biased strand distribution p-value = 8.05e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 230 total aligned reads displayed.

CACGCTGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGAATA  >  NC_000913/3608547‑3608844
                                                                                                                                                    |                                                                                                                                                     
   gCTGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTggg                                                                                                                                                   <  2:2590319/150‑1 (MQ=255)
   gCTGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTggg                                                                                                                                                   <  1:1376805/150‑1 (MQ=255)
      gTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGgag                                                                                                                                                <  2:3971596/150‑1 (MQ=255)
       ttAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGgaga                                                                                                                                               <  1:2761924/150‑1 (MQ=255)
        gaGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAACCCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGagag                                                                                                                                              <  2:2648945/149‑1 (MQ=255)
         aGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCTCCCCAGAGGGGCGAgg                                                                                                                                             <  2:3360845/150‑1 (MQ=255)
          gggATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAggg                                                                                                                                            <  1:1929035/150‑1 (MQ=255)
          gggATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAggg                                                                                                                                            >  1:2293583/1‑150 (MQ=255)
           ggATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGt                                                                                                                                           >  2:2568526/1‑150 (MQ=255)
           ggATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGt                                                                                                                                           >  1:493057/1‑150 (MQ=255)
           ggATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGt                                                                                                                                           >  1:1118307/1‑150 (MQ=255)
             aTGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTa                                                                                                                                         <  1:666829/150‑1 (MQ=255)
             aTGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTa                                                                                                                                         <  1:594726/150‑1 (MQ=255)
               gACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAgg                                                                                                                                       <  2:1283315/150‑1 (MQ=255)
                 ccGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGTGGGTTAgggg                                                                                                                                     >  1:3447568/1‑149 (MQ=255)
                  cGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTg                                                                                                                                    >  2:26116/1‑150 (MQ=255)
                      ttGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAggg                                                                                                                                <  2:1672149/150‑1 (MQ=255)
                      ttGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGAAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAggg                                                                                                                                <  1:1241752/150‑1 (MQ=255)
                           gctggcAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAgg                                                                                                                                 <  2:894782/144‑1 (MQ=255)
                           gctggcAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGaaaa                                                                                                                           >  1:2674286/1‑150 (MQ=255)
                               gcAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAggcg                                                                                                                       >  2:3042836/1‑150 (MQ=255)
                               gcAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGGTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAggcg                                                                                                                       <  2:3757320/150‑1 (MQ=255)
                                cAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTCCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAggcgg                                                                                                                      >  1:592372/1‑150 (MQ=255)
                                   tGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCAt                                                                                                                   >  2:2682902/1‑150 (MQ=255)
                                      tGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACAGTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGa                                                                                                                >  1:273369/1‑150 (MQ=255)
                                       ggCAGATAGGGGGGCGAGGGTGCTGGTGACAGCGAATGCTTTAAGATGGTGGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATGGGTCCCCTCGCCCCTCTGGGTTTTGGGTTTGGGTGTGGGGAAAAGGCGGCATCGaa                                                                                                               <  2:3098829/150‑1 (MQ=255)
                                       ggCAGATAGGGGGGCGACGGTGCTGGTGACTGGGATTGCTTTAAGATTGTTGCGCTGGTGATATGGAAAACCGATCGAAAATTTGAGCGCTATGGGTCCCCGCGCCCCTCTGGGTTGTGGTTTTGGGTGAGGGGAAAAGGCGGCATCGaa                                                                                                               <  2:3317985/150‑1 (MQ=255)
                                        gCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAg                                                                                                              >  2:3332991/1‑150 (MQ=255)
                                            aTACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCaa                                                                                                          >  2:3237078/1‑150 (MQ=255)
                                            aTACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCaa                                                                                                          >  2:1972215/1‑150 (MQ=255)
                                              cggggggCGCGGGTGGGGGTGACCGCGAATGCTTTAATTTTTTGGCGCAGGAGATATGCAAAACCGAGCGAAAAATTGAGCGCGCTCGGCCCCCTCGCCCCTGTGGGTAGTGGTTTAGGGTGTGGGGAAAAGGCGGCATCGAAGCCAATc                                                                                                        <  2:1867860/148‑1 (MQ=255)
                                              cggggggCGACGGTGCTGGTGACAGCTATTGCTTTATGATTGTTGCGCAGTAGATAAGGCAAACCGATCGAAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGTGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATc                                                                                                        <  2:1782144/148‑1 (MQ=38)
                                                ggggggGACGGTGCGGGTGACAGCTATTGGGTTATGTTGGTGGCTCTGGATAAATGCCAACCCGTGCGCAACTTTGTGCGCTATGGTGCCCCTCGCCCCTCTGGGGTGTGGGTTTGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAg                                                                                                      <  2:3771891/150‑1 (MQ=255)
                                                   ggCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGccc                                                                                                   >  1:3479207/1‑150 (MQ=255)
                                                   ggCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGccc                                                                                                   >  1:212044/1‑150 (MQ=255)
                                                         ggTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGCGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCa                                                                                             >  2:2693056/1‑150 (MQ=255)
                                                         ggTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCa                                                                                             >  1:2522134/1‑150 (MQ=255)
                                                         ggTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCa                                                                                             >  2:3438103/1‑150 (MQ=255)
                                                         ggTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCa                                                                                             >  1:4176436/1‑150 (MQ=255)
                                                            gCTGGTGACTGCTATTGCGTTACGATTGTGGCGCTGGTGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGTGTGGTTTTGGGTGCGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAAcc                                                                                          <  2:1006169/150‑1 (MQ=255)
                                                             cTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCg                                                                                         >  1:3019421/1‑150 (MQ=255)
                                                             cTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCg                                                                                         >  1:2903029/1‑150 (MQ=255)
                                                              tGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGc                                                                                        >  1:628230/1‑150 (MQ=255)
                                                               ggTGACAGCGAATGCTTTAAGATTGTTGCGCTGGAGATACGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGcc                                                                                       <  2:2419883/150‑1 (MQ=255)
                                                                gTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCt                                                                                      >  1:3819758/1‑150 (MQ=255)
                                                                 tGACAGCTAATGCTTTAATATTGTTGCGCAGGTTATAAGGAAACCCGATCGCAACTTTGCGCGCGATGGGTCCCCTCGCCCCTGGGGGGTGTGGTTTTGGGTGCGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCtt                                                                                     <  2:3659961/150‑1 (MQ=255)
                                                                 tGACAGCGATTGCGTTACGATGGTGGCGCAGGATAGACGGCAAACCGCGCGCAACATTGCGCGCGCTCGGTCCCCGCGCCCCTCTGGGGAGCGGGTTAGGGTGTGGGTAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCtt                                                                                     <  2:112929/150‑1 (MQ=255)
                                                                   aCAGCTAATGCTTTATGTTTGTGGCGCTGGATATATGGCAAACCGATCGCAACATTGCGCGCGATGGGTCCCCTCGCCCCTCTGGGGTGTGGGTTTGGGTGAGGGTAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTa                                                                                   <  2:1609723/150‑1 (MQ=255)
                                                                    cAGCGATGGCTTTACGTTTGTGGCGCAGGATATCAGGCAACCCGACCGAAACATTGCGCGCGATCGGCCCCCCCGCCCCGCTGGGGCGTGGTTTTGGGGGCGGGCACAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTAc                                                                                  <  1:2642075/150‑1 (MQ=255)
                                                                    cAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTAc                                                                                  >  2:2966624/1‑150 (MQ=255)
                                                                            gCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGTGAGGGTTCGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCaaa                                                                          <  2:2840464/150‑1 (MQ=255)
                                                                              gTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATa                                                                        >  1:848662/1‑150 (MQ=255)
                                                                              gTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTGAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTGTACGAATCAAATa                                                                        >  2:3564293/1‑150 (MQ=255)
                                                                               ttAAGTTTGTGGCGCTGTATATAAGCAAAACCTATCTAAACTTTGCGCGCGATCGGTCCCCCCGCCCCTGTGGGGTGTGGTTTGGGGTGCGGGTAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATaa                                                                       <  1:315643/150‑1 (MQ=255)
                                                                                   gATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGAt                                                                   >  1:2882903/1‑150 (MQ=255)
                                                                                       gttgCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTGTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAgg                                                               <  2:2726872/150‑1 (MQ=255)
                                                                                           cgcAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGt                                                           >  2:3624533/1‑150 (MQ=255)
                                                                                             cAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTcc                                                         >  1:2821101/1‑150 (MQ=255)
                                                                                             cAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTcc                                                         >  2:3323900/1‑150 (MQ=255)
                                                                                             cAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCGGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAAACAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTcc                                                         >  2:765837/1‑150 (MQ=255)
                                                                                              aGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCa                                                        >  2:404245/1‑150 (MQ=255)
                                                                                                       ggCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTctct                                               >  2:1962808/1‑150 (MQ=255)
                                                                                                        gCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCtt                                              >  1:3681300/1‑150 (MQ=255)
                                                                                                        gCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCtt                                              >  1:204301/1‑150 (MQ=255)
                                                                                                         cAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCttt                                             >  1:4060630/1‑150 (MQ=255)
                                                                                                          aaaCCGATCGCAACTTTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGTGAGGGTTAGGGTGTGGGTAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTa                                            <  2:4243644/150‑1 (MQ=255)
                                                                                                          aaaCCGATCGCAACATTGAGCGCGATGGGTCCCCTCGCCCCTCTGGGGTGAGGGTTCGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTa                                            <  2:2397363/150‑1 (MQ=255)
                                                                                                                    cAACTTTGCGCGGTATGGGTCCCCTCGCCCTTGTGGGTAGTTGTTTTGGGTGGGGGCAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGtt                                  <  1:3650008/150‑1 (MQ=255)
                                                                                                                     aaCTTTGCGCGCTATGGGACCCCTCGCCCCTGTGGGTTGTGGTTTTGGGTGGGGGTACAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTc                                 <  2:1271840/150‑1 (MQ=255)
                                                                                                                     aaCATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTc                                 >  2:1919862/1‑150 (MQ=255)
                                                                                                                       ctttGAGCGCGATCGGTCCCCTCGCCCCTCTGGGTTGTGTTTTTGGGTGGGGGGACAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCgt                               <  2:1285562/148‑1 (MQ=255)
                                                                                                                         ttGAGCGCGATCGGCCCCCTCCCCCCTCTGGGGAGTGGGTTCGGGTGCGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCgtgt                             <  2:822336/150‑1 (MQ=255)
                                                                                                                          tgggcgcGTGCGGTCCCCCCGCCCCGGTGGGGTGTGGGTTTGGGTGTGGGCACAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGtt                            <  1:3930887/147‑1 (MQ=255)
                                                                                                                           gAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTc                           >  1:1547638/1‑150 (MQ=255)
                                                                                                                            tgcgcGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAATGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTcg                          >  1:1021137/2‑149 (MQ=255)
                                                                                                                            cgcgcGATCGGTCCCCTCGCCCCTCTGGGGAGTGGGTTTGGGTGCGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTcc                          <  2:579023/149‑1 (MQ=255)
                                                                                                                            aGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTcc                          >  1:2134880/1‑150 (MQ=255)
                                                                                                                             gcgcGATCGGTCCCCTCGCCCCTCTGGGTTGTGGGTTCGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCa                         <  2:1733949/150‑1 (MQ=255)
                                                                                                                              cgcgATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCCATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCAt                        >  1:3886247/1‑150 (MQ=255)
                                                                                                                               gctaTCGGTCCCCTCGCCCCTCTGGGGAGTGGGTTAGGTTGAGGGTAAACGGCGGCATCGACGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATa                       <  1:3880397/147‑1 (MQ=39)
                                                                                                                               gcgTTCGGACCCCTCGCCCCTGTGGGGTGTGGGTTTGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATa                       <  1:3080907/150‑1 (MQ=255)
                                                                                                                                 gATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATaaa                     <  1:1401346/150‑1 (MQ=255)
                                                                                                                                 gATCGGTCCCCGCGCCCCTGTGGGGTGTGGGTTGGGGTGCGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATaaa                     <  1:3295472/150‑1 (MQ=255)
                                                                                                                                  ctCGGTCCCCTCGCCCCTCTGGGGAGTGGTTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGACACTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAg                    <  2:4149587/149‑1 (MQ=255)
                                                                                                                                  aTCGGACCCCTCGCCCCTGTGGTGTGTGGTTTTGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAg                    <  2:1518826/150‑1 (MQ=255)
                                                                                                                                      taacccTCTCCCCAGAGGGGCGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGACTCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTAc                >  1:867798/4‑150 (MQ=255)
                                                                                                                                       tCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACa               >  1:1729156/1‑150 (MQ=255)
                                                                                                                                          catCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACAGCCTTTACGAATCAAATAACTATAAGGCAGTCCATCCTTCTCTTTATCAACCAGTTCGTGTTCCATAAAGGTACaaaa            >  2:2173354/3‑150 (MQ=255)
                                                                                                                                           cTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAAc           >  2:477566/1‑150 (MQ=255)
                                                                                                                                           cTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTGTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCACCCAGTTCGTGTTCCATCACGGTCCAAAAc           >  1:3918162/1‑150 (MQ=39)
                                                                                                                                            tCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAAcc          >  2:103662/1‑150 (MQ=255)
                                                                                                                                            tCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAAcc          >  1:2504873/1‑150 (MQ=255)
                                                                                                                                            tCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAAcc          >  1:2792092/1‑150 (MQ=255)
                                                                                                                                              gCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCa        >  1:1001052/1‑150 (MQ=255)
                                                                                                                                              gCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCa        >  2:1377375/1‑150 (MQ=255)
                                                                                                                                              gCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCa        >  1:1055800/1‑150 (MQ=255)
                                                                                                                                              gCCCCGCTGGGTCGTGGTTTTGGGTGAGGGTAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCa        <  1:810034/150‑1 (MQ=255)
                                                                                                                                                cccTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAgg      >  2:3750787/1‑150 (MQ=255)
                                                                                                                                                cccTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAgg      >  2:2287416/1‑150 (MQ=255)
                                                                                                                                                   tctGGGGAGAGGGTTAGGGTGAGGGGAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGAata  >  2:416180/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CACGCTGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGAATA  >  NC_000913/3608547‑3608844

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: