Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,106,369 T→G 6.2% intergenic (+49/‑100) cpxP → / → fieF inhibitor of the cpx response; periplasmic adaptor protein/ferrous iron and zinc transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,106,3690TG 6.2% 787.8 / 33.1 263intergenic (+49/‑100)cpxP/fieFinhibitor of the cpx response; periplasmic adaptor protein/ferrous iron and zinc transporter
Reads supporting (aligned to +/- strand):  ref base T (119/125);  new base G (13/5);  total (133/130)
Fisher's exact test for biased strand distribution p-value = 8.50e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.29e-01

Only 100 of 266 total aligned reads displayed.

AGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGATTGCTG  >  NC_000913/4106220‑4106514
                                                                                                                                                     |                                                                                                                                                  
agaAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACAt                                                                                                                                                    <  2:2670853/150‑1 (MQ=255)
   aaCATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCt                                                                                                                                                 <  2:3592641/150‑1 (MQ=255)
   aaCATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCt                                                                                                                                                 >  1:3893868/1‑150 (MQ=255)
    aCATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCtg                                                                                                                                                >  1:1656545/1‑150 (MQ=255)
     cATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCtgt                                                                                                                                               >  2:1785327/1‑150 (MQ=255)
      aTCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCtgtg                                                                                                                                              <  1:1953136/150‑1 (MQ=255)
      aTCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCtgtg                                                                                                                                              <  1:2748004/150‑1 (MQ=255)
       tCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTgg                                                                                                                                             >  2:755499/1‑150 (MQ=255)
       tCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTgg                                                                                                                                             <  2:3996568/150‑1 (MQ=255)
        caacaaCGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACAGCATGTggg                                                                                                                                            <  2:487710/150‑1 (MQ=255)
        caacaaCGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACAGCATGTggg                                                                                                                                            <  2:4199965/150‑1 (MQ=255)
         aacaacGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTgggg                                                                                                                                           >  1:1308282/1‑150 (MQ=255)
          acaacGAATGGAGCAGTTGCGTGACGTGACGCACTGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACAGCATGTggggg                                                                                                                                          <  2:1679545/150‑1 (MQ=255)
            aacGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGtt                                                                                                                                       >  2:846859/1‑150 (MQ=255)
                  tGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGtttttttt                                                                                                                                 <  2:825186/150‑1 (MQ=255)
                  tGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGtttttttt                                                                                                                                 <  2:2616916/150‑1 (MQ=255)
                   ggAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTa                                                                                                                                <  2:664948/150‑1 (MQ=255)
                    gAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTAt                                                                                                                               <  1:430671/150‑1 (MQ=255)
                     aGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATc                                                                                                                              >  2:1111966/1‑150 (MQ=255)
                     aGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATc                                                                                                                              >  2:2348704/1‑150 (MQ=255)
                      gCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATcc                                                                                                                             <  2:3322612/150‑1 (MQ=255)
                              gtgacgtgacGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTg                                                                                                                     >  1:4339199/1‑150 (MQ=255)
                                 acgtgacgCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCct                                                                                                                  <  2:3675169/150‑1 (MQ=255)
                                 acgtgacgCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCct                                                                                                                  <  2:1933357/150‑1 (MQ=255)
                                    tgacgCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCGGTGGGGGTTTTTTTTATCCTCAATTTGCctgct                                                                                                               >  2:4605751/1‑150 (MQ=255)
                                          aaTGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTaa                                                                                                         <  1:1526405/150‑1 (MQ=255)
                                               cAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTATTTTTTATCCTCAATTTGCCTGCTGCTTAATGCAt                                                                                                    <  1:3209291/150‑1 (MQ=255)
                                                aaaaaaGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCAtt                                                                                                   <  2:1713938/150‑1 (MQ=255)
                                                 aaaaaGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTg                                                                                                  >  1:1207528/1‑150 (MQ=255)
                                                  aaaaGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGc                                                                                                 <  2:2781512/150‑1 (MQ=255)
                                                  aaaaGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGc                                                                                                 <  2:4439276/150‑1 (MQ=255)
                                                  aaaaGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCGGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGc                                                                                                 <  2:2781738/150‑1 (MQ=255)
                                                        tCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAgatga                                                                                           <  1:3481071/150‑1 (MQ=255)
                                                        tCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAgatga                                                                                           <  2:4361335/150‑1 (MQ=255)
                                                          aTCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGAtt                                                                                         >  1:1063496/1‑150 (MQ=255)
                                                          aTCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAAAACTGTATTCCTTGCCATAGACACCATCCCTGTCGTCCCCCACATGCTGTGGCGGTATTTTTTATCCACAATTTGACTGCTGCTTATAGCAGTGCAGATGAtt                                                                                         >  1:261337/1‑150 (MQ=255)
                                                           tCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGAttt                                                                                        <  2:2624970/150‑1 (MQ=255)
                                                             gTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGc                                                                                      <  2:3489858/150‑1 (MQ=255)
                                                               tGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCtt                                                                                    <  2:2181082/150‑1 (MQ=255)
                                                               tGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCtt                                                                                    <  2:3122012/150‑1 (MQ=255)
                                                                 aaGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTcc                                                                                  <  2:2928306/150‑1 (MQ=255)
                                                                 aaGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTcc                                                                                  <  2:114422/150‑1 (MQ=255)
                                                                    cTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGtt                                                                               >  1:4007029/1‑150 (MQ=255)
                                                                       ttGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTtata                                                                            <  1:919068/150‑1 (MQ=255)
                                                                        tGAGTAGTAGCAACTCACGTTCCCAGTAGTAACCCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACCTGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTTCAGATGATTTGCTTCCGTTATAc                                                                           <  2:2363229/150‑1 (MQ=255)
                                                                          agtagtAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTataata                                                                         >  2:3094891/1‑150 (MQ=255)
                                                                            tagtagCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGc                                                                       <  2:255688/150‑1 (MQ=255)
                                                                            tagtagCAACTCACGTTCCCAGTAGGAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTATTGCATTGCAGATGATTTGCTTCCGTTATACTAGc                                                                       <  2:1064283/150‑1 (MQ=255)
                                                                              gtagCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGt                                                                     >  1:1394284/1‑150 (MQ=255)
                                                                               tagCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTc                                                                    >  1:208966/1‑150 (MQ=255)
                                                                               tagCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACAGGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCGTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTc                                                                    >  1:4460997/1‑150 (MQ=255)
                                                                                   aaCTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGtt                                                                >  2:3830206/1‑150 (MQ=255)
                                                                                         cGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGc                                                          <  1:3560804/150‑1 (MQ=255)
                                                                                          gTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCg                                                         >  1:4790484/1‑150 (MQ=255)
                                                                                           ttCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCgg                                                        <  1:2582347/150‑1 (MQ=255)
                                                                                                agtagtAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACAAGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCAGCTGCTTAATGCATTTCAGATGATTTGCTTCCGTTATAATAGCGTCAGTTGATAGCGGGAGTa                                                   >  1:543329/1‑150 (MQ=255)
                                                                                                       aaCCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGa                                            >  2:3448256/1‑150 (MQ=255)
                                                                                                       aaCCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGa                                            >  1:755613/1‑150 (MQ=255)
                                                                                                       aaCCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGa                                            >  2:1292902/1‑150 (MQ=255)
                                                                                                       aaCCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCACCCACATGATGTGGGGGTTTTTTTTATCATCAATATGACTGCTTCTTAATTCATTGCAGATGATTTGCTTACGTTATACTATCGAAAGATTATAGCTGAAGTAATTAtta                                            >  1:2588829/1‑148 (MQ=255)
                                                                                                         cccTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGaat                                          <  2:4250629/150‑1 (MQ=255)
                                                                                                         cccTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGaat                                          <  2:3938682/150‑1 (MQ=255)
                                                                                                          ccTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGTTGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGaatc                                         >  1:4225141/1‑150 (MQ=255)
                                                                                                          ccTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGaatc                                         >  1:2980947/1‑150 (MQ=255)
                                                                                                          ccTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGaatc                                         >  2:1520923/1‑150 (MQ=255)
                                                                                                            tGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGaatcaa                                       >  2:3543705/1‑150 (MQ=255)
                                                                                                            tGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAAGTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGaatcaa                                       >  2:3002893/1‑150 (MQ=255)
                                                                                                                   cTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATg                                >  2:2054931/1‑150 (MQ=255)
                                                                                                                   cTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATg                                <  2:699403/150‑1 (MQ=255)
                                                                                                                        cATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACgg                           <  2:3587130/150‑1 (MQ=255)
                                                                                                                         aTAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGc                          >  2:4054004/1‑150 (MQ=255)
                                                                                                                         aTAGACACCATCCCGGTCTGCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATGTGCTTCCGGTATACTAGCGTCAGGTGATAGCGGGCGTATTTATGAATCAATCTTATGGACGGc                          >  2:4444187/1‑150 (MQ=255)
                                                                                                                          tAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCt                         <  2:1826941/150‑1 (MQ=255)
                                                                                                                           aGACACCATCCCTGTCTTCCCCCACATGCGGTGGGGGTTTTTTTTATACTCAATTTTCCTGCTGCTTAATTCATTGCAGATGATTTTCTTCAGTTATACTATCGTCAGTTGATAGCGGGAGTATTTATGTATCAAACTTATTGAAGGCTg                        >  1:4147706/1‑150 (MQ=255)
                                                                                                                            gACCCCATCCCTGTCTTCCCCCACCGGCTGGGGGGGTTTTTTGGATCCTCACTGTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATCCTCGCGTCAGTTGATAGCGGGAGTATTTATGCATCAATCTTATGGACGGCTgg                       <  2:568011/150‑1 (MQ=255)
                                                                                                                            gACACCATCCCTGTCTTCCCCCCCCTGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGAGGATTTGCTTCCGTTATACGAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTgg                       <  1:2921041/150‑1 (MQ=255)
                                                                                                                            gACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTgg                       <  1:2178594/150‑1 (MQ=255)
                                                                                                                             acacCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGt                      <  1:3313852/150‑1 (MQ=255)
                                                                                                                             acacCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGt                      <  1:1743205/150‑1 (MQ=255)
                                                                                                                               acCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCa                    >  2:3418956/1‑150 (MQ=255)
                                                                                                                               acCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCa                    >  2:4638479/1‑150 (MQ=255)
                                                                                                                               acCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATAAAAAAATTTCCTGCTGATTAATTAATTTCAGATTATTTGGTTAAGTTATAATATCGTCAGGTGATAGCGGGAGTATTTATGAATCAATCTTATTGACGGCTGGtaa                    >  1:3811232/1‑148 (MQ=255)
                                                                                                                                  aTCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTc                 >  2:3741158/1‑150 (MQ=255)
                                                                                                                                         tCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGggcggc          >  2:1419374/1‑150 (MQ=255)
                                                                                                                                         tCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGggcggc          <  1:3992410/150‑1 (MQ=255)
                                                                                                                                           ttCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGa        >  1:4553781/1‑150 (MQ=255)
                                                                                                                                           ttCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGa        >  1:2509604/1‑150 (MQ=255)
                                                                                                                                           ttCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGa        >  1:2304239/1‑150 (MQ=255)
                                                                                                                                           ttCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGa        >  1:3135384/1‑150 (MQ=255)
                                                                                                                                            tCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGAt       <  1:1683680/150‑1 (MQ=255)
                                                                                                                                              ccccACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGATtg     <  2:4491901/150‑1 (MQ=255)
                                                                                                                                              ccccACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGATtg     >  1:4744756/1‑150 (MQ=255)
                                                                                                                                              ccccACAGCATGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGATtg     >  1:2797594/1‑150 (MQ=255)
                                                                                                                                              ccccACAGCATGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGGCGGGCGGCGATtg     >  2:1287777/1‑150 (MQ=255)
                                                                                                                                              ccccACAGCATGTGGGGGTTTTTTTTATCCTAAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGATtg     >  1:4368791/1‑150 (MQ=255)
                                                                                                                                               cccACATGCTGTGGGGGATTTTTTTTATCCGAAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATCAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGCTtg     >  2:2505345/1‑150 (MQ=255)
                                                                                                                                               cccACATGCTGTGGGGGTTTTTTTTATACTCAATTTTCCTGCTGCTTAATTCATTTCAGATGATTTGCTTCCGTTATACAATCGTCAGTTGATAGCGGGAGTATTTATGAATAAAACTTATGTACGGCTGGTCAGTCGGGCGGCGAtttc    >  1:1092035/1‑148 (MQ=255)
                                                                                                                                                ccACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGATtgct   >  1:3103608/1‑150 (MQ=255)
                                                                                                                                                ccACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGATtgct   <  2:3906790/150‑1 (MQ=255)
                                                                                                                                                ccACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGATGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGAGTCAGTTGATAGCGGGAGGATTTATGAATCAATCTTATGGACGGCGGGTCAGTCGGGCGGCGATtgct   >  2:3361560/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
AGAAACATCAACAACGAATGGAGCAGTTGCGTGACGTGACGCAATGGCAAAAAAGTTCATCGTTGAAGCTATTGAGTAGTAGCAACTCACGTTCCCAGTAGTAAACCCTGTTTTCCTTGCCATAGACACCATCCCTGTCTTCCCCCACATGCTGTGGGGGTTTTTTTTATCCTCAATTTGCCTGCTGCTTAATGCATTGCAGATGATTTGCTTCCGTTATACTAGCGTCAGTTGATAGCGGGAGTATTTATGAATCAATCTTATGGACGGCTGGTCAGTCGGGCGGCGATTGCTG  >  NC_000913/4106220‑4106514

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: