Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 380,005 A→C 7.1% intergenic (‑124/+64) frmR ← / ← yaiO regulator protein that represses frmRAB operon/outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913380,0050AC 7.1% 449.5 / 2.0 154intergenic (‑124/+64)frmR/yaiOregulator protein that represses frmRAB operon/outer membrane protein
Reads supporting (aligned to +/- strand):  ref base A (119/19);  new base C (2/13);  total (122/32)
Fisher's exact test for biased strand distribution p-value = 1.16e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 164 total aligned reads displayed.

TTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTACCTGCCGT  >  NC_000913/379856‑380152
                                                                                                                                                     |                                                                                                                                                      
ttcttcTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACaaa                                                                                                                                                        <  2:1361416/150‑1 (MQ=255)
 tcttctcttcCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAag                                                                                                                                                       <  2:3710513/150‑1 (MQ=255)
 tcttctcttcCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAag                                                                                                                                                       <  1:225405/150‑1 (MQ=255)
 tcttctcttcCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAag                                                                                                                                                       <  2:3067168/150‑1 (MQ=255)
     ctcttcCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGa                                                                                                                                                   <  1:4249068/150‑1 (MQ=255)
       cttcCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGaaa                                                                                                                                                <  2:1061804/150‑1 (MQ=255)
        ttcCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTCTTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGCAA‑‑g                                                                                                                                             >  2:3384721/1‑150 (MQ=255)
         tcCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑gg                                                                                                                                            <  2:160798/150‑1 (MQ=255)
           cGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑gggg                                                                                                                                          >  1:4430757/1‑150 (MQ=255)
              tgTACGGGGAATTTCGCACCTCATCATCTGCATGCAATATACTATCGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGTAAAACAAAGAGGAAA‑‑ggggggg                                                                                                                                       <  1:2896567/149‑1 (MQ=255)
                 tcTGGGCATTTCGCACCTCATCATATGAATGCAATTTACTATTGGGGGGTTTTCTATATTTCAATGAATACCCGCCTATAGTATATAACACTTTAGAATAAATTTTTTCCGTTGAATGTGGTCGTAAAACAAAGAGGAAA‑‑gggggggggg                                                                                                                                    <  2:4578666/149‑1 (MQ=255)
                   tGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCt                                                                                                                                  >  1:3436351/1‑150 (MQ=255)
                   tGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAAAAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCt                                                                                                                                  >  2:2154707/1‑150 (MQ=255)
                    gggCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATAAAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTa                                                                                                                                 >  1:2513815/1‑150 (MQ=255)
                          ttCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGc                                                                                                                           >  1:4053251/1‑150 (MQ=255)
                           tCGCACCTTATCAGCTGCATGCAATTTACTATTGGGGGGTATTCTATATGTCAATGCATACCCCCCTTTAGTATATAACACTGGTGATTAAAATTTCTCCGGTGACTGTGGTCGGAAACCACCGAGGAAA‑‑GGGGGGGGGGCTAATCGGCa                                                                                                                          <  2:1417409/150‑1 (MQ=255)
                           tCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGCTAATCGGc                                                                                                                           >  2:2691705/1‑150 (MQ=255)
                              cACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAggg                                                                                                                       >  2:2567588/1‑150 (MQ=255)
                               aCCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGa                                                                                                                      >  2:1467587/1‑150 (MQ=255)
                               aCCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGa                                                                                                                      >  2:4461890/1‑150 (MQ=255)
                               aCCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGa                                                                                                                      >  2:410793/1‑150 (MQ=255)
                                     tcatcTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGgccg                                                                                                                >  1:1883310/1‑150 (MQ=255)
                                     tcatcTGCATGCAATATACTATAGGGGGGTAGTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGCATGTGGTCGGACAACAAAGAGGACA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGgccg                                                                                                                >  2:3845974/1‑150 (MQ=255)
                                      catcTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGgccgc                                                                                                               >  2:4053593/1‑150 (MQ=255)
                                        tcTGCATTCAATATACTCTAGGGGGTTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGTATAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGCGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGccc                                                                                                             <  2:4300304/150‑1 (MQ=255)
                                        tcTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGCGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGccc                                                                                                             >  2:2225715/1‑150 (MQ=255)
                                          ttcatgcaATATACTCTAGGGGGGTATTCTATATGTCCAGGCAAACCCCCCTATAGTATATACCACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAACGCGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCg                                                                                                           <  2:1426784/148‑1 (MQ=255)
                                          ttcatgcaATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCg                                                                                                           >  2:2028221/3‑150 (MQ=255)
                                          ttcatgcaATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCg                                                                                                           >  2:842473/3‑150 (MQ=255)
                                          ttcatgcaATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCGACGCGGCAGGGAAGGCCGCCCCg                                                                                                           >  2:3890769/3‑150 (MQ=255)
                                          ggcatccaATATACTTTAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATACCACTGGAGAATAAAATTTATCCTGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCg                                                                                                           <  2:4628287/149‑1 (MQ=255)
                                           gcatgcaATATCCTAGAGGGGGGTATTCAATATGTCAATGCATACCCCCCTATAGTATATAACACTGCAGAATAAAATCTAACCGGGGAATGGGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCgg                                                                                                          <  2:194841/150‑1 (MQ=255)
                                            catgcaATATACTATCGGGGGCTATTCTATATGTCAATGCATACCCCCCTATAGTAAACACCACTGGAGAATAAAATTTAGCCGGTGAATGTGGTCGGAAAACACAGAGGAAA‑‑GGGGGGGGGGCTACTCGGCAGGGAAGGCCGCCCCGGa                                                                                                         <  2:2371567/150‑1 (MQ=255)
                                            catgcaATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCCGGGAAGGCCGCCCCGGa                                                                                                         >  1:703893/1‑150 (MQ=255)
                                            catgcaATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGa                                                                                                         >  1:574225/1‑150 (MQ=255)
                                            catgcaATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGa                                                                                                         >  1:703859/1‑150 (MQ=255)
                                             atgcaATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATa                                                                                                       >  1:363783/1‑150 (MQ=255)
                                              tgcaATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGACA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATa                                                                                                       >  1:2787595/1‑150 (MQ=255)
                                              tgcaATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATa                                                                                                       >  1:723909/1‑150 (MQ=255)
                                                 aaTATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgcg                                                                                                    >  1:405963/1‑150 (MQ=255)
                                                 aaTATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCACTATAGTATATAACAATGGAGAATAAAATTTATAATGTGAATTTGGTCGGAAAACAAAGAGGAAA‑‑GGTGTGGGGGCTAATCGTCAGGGAAGGACGCCACGGATAgcg                                                                                                    >  2:1339588/1‑150 (MQ=255)
                                                     tcccctaGGGGGGTACTCTCTAGGACCATGCATACCCCCCTATACTAAATAACCCCGGAGACTAACATCTATCCGGCGCACGTGGCCGGAAAAACCCGAGGCAC‑‑GGGGGGGGGGCTCATCGGCAGGGAAGGCCGCCCCGGATAgcgggcg                                                                                                <  2:539685/145‑1 (MQ=255)
                                                        tataGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAGAGGGGAAG‑‑GGGGGGGGGGCTCATCGGCCGGGAAGGCCGCCCCGGATAGCGggcggcc                                                                                             >  2:4312605/1‑149 (MQ=255)
                                                        tataGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCa                                                                                             >  1:2640005/1‑150 (MQ=255)
                                                        tataGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCa                                                                                             >  2:2896649/1‑150 (MQ=255)
                                                         ataGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGa                                                                                           <  2:272663/150‑1 (MQ=255)
                                                                ggTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGGAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAAt                                                                                     >  2:934119/1‑150 (MQ=255)
                                                                ggTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAAt                                                                                     >  2:1586598/1‑150 (MQ=255)
                                                                ggTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAAt                                                                                     >  1:3877328/1‑150 (MQ=255)
                                                                ggTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGAGGGCGGCAGAAGGAAt                                                                                     >  1:4113067/1‑150 (MQ=255)
                                                                  tatactatacGCCAATGCACACCCCCCTATCGCATATACCACGGGAGAATAAACCCCCCCCGGTGAAGGGGGCCGGACACCACCGCCCACC‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCa                                                                                   <  2:2014266/150‑1 (MQ=255)
                                                                     tCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGACTCAGaa                                                                                >  1:935701/1‑150 (MQ=255)
                                                                      cTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAAt                                                                               >  1:2099971/1‑150 (MQ=255)
                                                                      cTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAAt                                                                               >  1:1448312/1‑150 (MQ=255)
                                                                      cTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAAt                                                                               >  2:3462406/1‑150 (MQ=255)
                                                                      cTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAAt                                                                               >  1:2100070/1‑150 (MQ=255)
                                                                       tataTGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGACA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAAtt                                                                              >  2:2039730/1‑150 (MQ=255)
                                                                       tataTGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGCTAAGCGGCCGGGAAGGCCGCCCCGGGAAGCGGGCGGAAGAAGGAAGAAGAAt                                                                               >  2:830618/1‑150 (MQ=255)
                                                                       tataTGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAAtt                                                                              >  2:2445340/1‑150 (MQ=255)
                                                                       tataTGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAACGAATCAGAAtt                                                                              >  1:2582528/1‑150 (MQ=255)
                                                                         tatGTCAATGCATACACCCCTATATTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTc                                                                            >  2:3243322/1‑150 (MQ=255)
                                                                            gTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAg                                                                         >  1:1882443/1‑150 (MQ=255)
                                                                             tCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAgg                                                                        >  2:4173164/1‑150 (MQ=255)
                                                                             tCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAgg                                                                        >  2:3251625/1‑150 (MQ=255)
                                                                               aaTGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTTAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTc                                                                      >  2:118476/1‑150 (MQ=255)
                                                                               aaTGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTc                                                                      >  1:503771/1‑150 (MQ=255)
                                                                               aaTGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTc                                                                      >  1:1352804/1‑150 (MQ=255)
                                                                               aaTGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTc                                                                      >  2:730221/1‑150 (MQ=255)
                                                                                aTGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCa                                                                     >  1:70825/1‑150 (MQ=255)
                                                                                   cATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGAc                                                                  >  2:1984239/1‑150 (MQ=255)
                                                                                   cATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGAc                                                                  >  2:4001838/1‑150 (MQ=255)
                                                                                       ccccccTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGc                                                              >  2:4101042/1‑150 (MQ=255)
                                                                                       ccccccTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGCCTAATCGGCAGGGAAGGCCGCCCCGGAAAGCGGGCGGCTGAAGGAATCAGAATTTCCAGGACAGACGGGc                                                              >  2:1223543/1‑150 (MQ=255)
                                                                                        cccccTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGACGGAATCAGAATTTCCAGGTCAGACGGGCt                                                             >  1:3690090/1‑150 (MQ=255)
                                                                                        cccccTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCt                                                             >  1:1848803/1‑150 (MQ=255)
                                                                                        cccccTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCt                                                             >  2:751703/1‑150 (MQ=255)
                                                                                        cccccTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCt                                                             >  1:474831/1‑150 (MQ=255)
                                                                                               taGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGACTCAGAATTTCCAGGTCAGACGGGCTGCAAGTTg                                                     >  1:886016/1‑150 (MQ=255)
                                                                                                    tataACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGa                                                 >  1:1040325/1‑150 (MQ=255)
                                                                                                    tataACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGa                                                 >  1:1040324/1‑150 (MQ=255)
                                                                                                       aaCACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCg                                              >  1:4295726/1‑150 (MQ=255)
                                                                                                            tGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGACTCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTaaa                                         >  2:3909273/1‑150 (MQ=255)
                                                                                                            tGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTaaa                                         >  2:2698618/1‑150 (MQ=255)
                                                                                                            tGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTaaa                                         >  1:1361297/1‑150 (MQ=255)
                                                                                                            tGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTACCCGGTCAGACGGGCTGCAAGTTGCAGCCCGGTaaa                                         >  2:1720445/1‑150 (MQ=255)
                                                                                                             ggAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAACG‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTGaaa                                         >  2:3174002/1‑150 (MQ=255)
                                                                                                             ggAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCCGGTCAGACGGGCTGCAAGTTGCAGACCGTTaaaa                                        >  1:2238987/1‑150 (MQ=255)
                                                                                                             ggAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTACAGGTCAGACGGGCTGCAAGTTGCAGACCGTTaaaa                                        >  1:3351056/1‑150 (MQ=255)
                                                                                                                   tAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCg                                  >  1:1637102/1‑150 (MQ=255)
                                                                                                                     aaaTTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGt                                >  1:2431500/1‑150 (MQ=255)
                                                                                                                           aTCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGgt                          >  2:1508351/1‑150 (MQ=255)
                                                                                                                           aTCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGgt                          >  2:2896372/1‑150 (MQ=255)
                                                                                                                           aTCCGGTGAATGGGGTCGGAAAACAACGAGGACA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCACAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGgt                          >  2:1508371/1‑150 (MQ=255)
                                                                                                                               ggTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGGGCTAAACGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCCGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGgtgt                        >  2:2266562/1‑150 (MQ=255)
                                                                                                                               ggTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCg                      >  1:1482573/1‑150 (MQ=255)
                                                                                                                                gTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGt                     >  1:2988617/1‑150 (MQ=255)
                                                                                                                                 cgAATGTGGTCGCAAAACCACGCCGAAC‑‑GGGGGGGGGGCTACTCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTa                    <  2:3701508/149‑1 (MQ=255)
                                                                                                                                    acctgGTCGGAAACCAACGCGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACca                 <  2:631648/147‑1 (MQ=255)
                                                                                                                                       tgGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCacac              >  1:2002724/1‑150 (MQ=255)
                                                                                                                                            ggAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTAACACACTTTAc         >  2:4190772/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
TTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTACCTGCCGT  >  NC_000913/379856‑380152

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: