Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 729,379 A→C 6.1% intergenic (+39/‑204) ybfA → / → rhsC DUF2517 family protein/Rhs protein with putative toxin domain; putative neighboring cell growth inhibitor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913729,3790AC 6.1% 738.3 / 29.7 212intergenic (+39/‑204)ybfA/rhsCDUF2517 family protein/Rhs protein with putative toxin domain; putative neighboring cell growth inhibitor
Reads supporting (aligned to +/- strand):  ref base A (114/85);  new base C (2/11);  total (116/96)
Fisher's exact test for biased strand distribution p-value = 3.73e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.19e-01

Only 100 of 214 total aligned reads displayed.

GGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTAAGGCTTTCTTATAAAT  >  NC_000913/729231‑729527
                                                                                                                                                    |                                                                                                                                                    
ggAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAAc                                                                                                                                                     >  1:3244069/1‑150 (MQ=255)
 gAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACg                                                                                                                                                    >  1:1754152/1‑150 (MQ=255)
  aaaGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGc                                                                                                                                                   >  1:3533333/1‑150 (MQ=255)
   aaGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCt                                                                                                                                                  <  1:2551776/150‑1 (MQ=255)
     gACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTgg                                                                                                                                                <  2:1360232/150‑1 (MQ=255)
      aCCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGc                                                                                                                                               >  1:928743/1‑150 (MQ=255)
        cgcgCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGTTTCCGCTGGCgg                                                                                                                                             <  2:110972/150‑1 (MQ=255)
         gcgcCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTTATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGt                                                                                                                                            >  1:2063405/1‑150 (MQ=255)
         gcgcCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGt                                                                                                                                            >  1:4442635/1‑150 (MQ=255)
         gcgcCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGt                                                                                                                                            >  1:2407272/1‑150 (MQ=255)
         gcgcCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGt                                                                                                                                            <  2:2846766/150‑1 (MQ=255)
          cgcCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGTTTCCGCTGGCGGtt                                                                                                                                           <  2:4006173/150‑1 (MQ=255)
          cgcCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGTTTCCGCTGGCGGtt                                                                                                                                           <  1:2984837/150‑1 (MQ=255)
          cgcCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGtt                                                                                                                                           <  2:2715828/150‑1 (MQ=255)
           gcCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGTTTCCGCTGGCGGttt                                                                                                                                          <  2:1624837/150‑1 (MQ=255)
           gcCCGCTATTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGAGTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGTTTCCGCTGGCGGttt                                                                                                                                          <  2:3243793/150‑1 (MQ=255)
            cccGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGtttt                                                                                                                                         >  1:3433429/1‑150 (MQ=255)
              cGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTTTTGATTGCTTAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGtttttt                                                                                                                                       <  1:2991755/150‑1 (MQ=255)
               gCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTg                                                                                                                                      >  2:2776402/1‑150 (MQ=255)
                    tACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTAATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCa                                                                                                                                 <  2:3706853/150‑1 (MQ=255)
                     aCAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAg                                                                                                                                >  2:2994077/1‑150 (MQ=255)
                      cAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGCGTGGTTGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGATTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAgg                                                                                                                               <  1:3582711/150‑1 (MQ=255)
                         cTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAg                                                                                                                            >  2:1573462/1‑150 (MQ=255)
                         cTACCGGCATCGCGTCTGGTCGAAACCCAGCGATAAACCGGGGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAg                                                                                                                            <  2:4121155/150‑1 (MQ=255)
                            ccTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTTTCAGGTAGATg                                                                                                                         >  1:4027961/1‑150 (MQ=255)
                             cTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGt                                                                                                                        >  1:2632882/1‑150 (MQ=255)
                              tGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGtt                                                                                                                       >  2:3615449/1‑150 (MQ=255)
                                  tCGCGTCTTGTAGAAAACCAGCTATAAACCGTTGTTGATTTATCTGGCCGAAATGTAAAATTGTGATTTTTTTTGTTTGCTGATTTTTTAATACTTTGAAAACCGCAAGCGGAAACGGTGGCGGTTTTTTGTATCAGGAAGATGTTCaga                                                                                                                   <  1:1489335/150‑1 (MQ=255)
                                  tCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCTAAAAGGCAACTGGTTATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCaga                                                                                                                   <  1:2798607/150‑1 (MQ=255)
                                       tCTGGTCTAAAACCAGCTATAAACCGGTGTGGATTGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAat                                                                                                              <  1:2327107/150‑1 (MQ=255)
                                          ggTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATAtt                                                                                                           <  2:2900616/150‑1 (MQ=255)
                                          ggTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTATATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATAtt                                                                                                           <  2:3798556/150‑1 (MQ=255)
                                           gTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATAttt                                                                                                          >  2:1664288/1‑150 (MQ=255)
                                           gTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATAttt                                                                                                          >  1:474258/1‑150 (MQ=255)
                                            tCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTa                                                                                                         >  2:1498935/1‑150 (MQ=255)
                                            tCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTa                                                                                                         >  2:1678942/1‑150 (MQ=255)
                                             cGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGTTTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAg                                                                                                        <  1:2144665/150‑1 (MQ=255)
                                             cGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAg                                                                                                        <  1:1592762/150‑1 (MQ=255)
                                              gAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGa                                                                                                       >  2:3454565/1‑150 (MQ=255)
                                                   ccAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGAAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCg                                                                                                  <  1:3646894/150‑1 (MQ=255)
                                                    cAGCGATAAACCGGTGTGGATGGATCAGTCCGAAAAGGCAACTGGTGATATTAATAGATTGCTGAGATTTGAATACATTGAAATCCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCgg                                                                                                 >  2:4211940/1‑149 (MQ=255)
                                                    cAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGACTATTTAGACATCGt                                                                                                 >  1:1237974/1‑150 (MQ=255)
                                                    cAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGt                                                                                                 >  2:1184588/1‑150 (MQ=255)
                                                    cAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATAAGGAAGATGTTCAGAGGAATATTTAGACATCgg                                                                                                 >  1:3811380/1‑149 (MQ=255)
                                                     aGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGtt                                                                                                >  1:4478180/1‑150 (MQ=255)
                                                     aGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGtt                                                                                                >  1:3758634/1‑150 (MQ=255)
                                                      gCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTta                                                                                               <  2:2036272/150‑1 (MQ=255)
                                                        gATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTtata                                                                                             >  1:3038295/1‑150 (MQ=255)
                                                          tAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATAcc                                                                                           >  2:3076040/1‑150 (MQ=255)
                                                          tAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATAcc                                                                                           >  2:1985655/1‑150 (MQ=255)
                                                          tAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATAGTtataac                                                                                           >  2:2521655/1‑148 (MQ=255)
                                                            aaCCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCaa                                                                                         >  2:2286835/1‑150 (MQ=255)
                                                            aaCCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCaa                                                                                         >  2:2104308/1‑150 (MQ=255)
                                                               cGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCg                                                                                      >  2:697028/1‑150 (MQ=255)
                                                                  tgtgGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGaaa                                                                                   <  1:650778/150‑1 (MQ=255)
                                                                   gtgGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAAc                                                                                  >  2:4153734/1‑150 (MQ=255)
                                                                   gtgGAAGGATCAGGCCGAAAAGGCAACTGGTGATATTTAATGATTGCTGAGTTATGAATACATAGAAAACCGCCAGCGGAAACCCTGGCGCAATATAGAATCACGAAGATGTTCACAGGAATATATAGACATCGTTATAACAATCGAAAc                                                                                  >  2:3658268/1‑150 (MQ=255)
                                                                    tggatggaTCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGAAATCGGTATACCAAACGAAACa                                                                                 >  1:1832080/1‑150 (MQ=255)
                                                                     ggatggatCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACat                                                                                <  2:2760631/150‑1 (MQ=255)
                                                                         ggatCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATAACTTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCGGGAAGATGTTCAGAGGAATATTTAGACATCGTTATTCGACTCGAAACatagag                                                                            >  2:2905410/1‑147 (MQ=255)
                                                                             cAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCAt                                                                        <  1:3356582/150‑1 (MQ=255)
                                                                             cAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCAt                                                                        <  1:4544900/150‑1 (MQ=255)
                                                                                gCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGaa                                                                     >  2:854198/1‑150 (MQ=255)
                                                                                gCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGaa                                                                     >  1:1921511/1‑150 (MQ=255)
                                                                                 ccGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATAACAATCGAAACATATATTAATGaaa                                                                    >  1:2218695/1‑150 (MQ=255)
                                                                                  cGAAAAGGCAACAGGTGATTTTTATTGATTCCTGAGTTTTGAAAACAAAGAAAACCGCCAGCGGAAACGCTGGCGCTTATATGTATCAGGAAGATGTTCAGACCAAAAAATAGACATCGTTATACCAATCGAAACATATATTCATGaaaa                                                                   >  2:1474179/1‑149 (MQ=255)
                                                                                     aaaGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAatata                                                                <  1:3489132/150‑1 (MQ=255)
                                                                                        ggCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGACACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAatatatat                                                             >  1:1437339/1‑150 (MQ=255)
                                                                                           aaCTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAAAAAATATTCATGAAATATATATaaa                                                          >  1:1301455/1‑150 (MQ=255)
                                                                                              tGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACCTCGTTATACCAATCGAAACATATATTAATGAAATATATATAAatat                                                       >  1:602586/1‑150 (MQ=255)
                                                                                                  gATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTATTTTGTATCAGGACGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTc                                                   <  2:518289/150‑1 (MQ=255)
                                                                                                  gATTTAGATTGATTGCTGAGTTTTGAATCCATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTc                                                   <  2:4205533/150‑1 (MQ=255)
                                                                                                   aTTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCACCGGAAACCCAGGCGGTTTTTTGTATCAGGAAGAGGTTCACAGGAATATTTAGAAATCGTTATACCAAACGAAACATATAATCATGAAAAAAATATAAAAAttttac                                                  >  1:197638/1‑148 (MQ=255)
                                                                                                      tttATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTTTATAAGGAAGATGTTAAGAGGAAAATTTAGAAAAAGTTATAACAAAAGAAAAAAATAATCATTAAATATATATAAATATTTTACTaa                                               >  1:4058934/1‑150 (MQ=255)
                                                                                                      tttATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTaa                                               >  2:4258617/1‑150 (MQ=255)
                                                                                                       ttATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTCTCCTAAt                                              <  1:2432383/150‑1 (MQ=255)
                                                                                                        tATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAAtt                                             <  2:2063405/150‑1 (MQ=255)
                                                                                                        tATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAAtt                                             <  2:4442526/150‑1 (MQ=255)
                                                                                                        tATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAAtt                                             <  2:2407272/150‑1 (MQ=255)
                                                                                                            gattgCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATAAGGAAGATGTTCAGAGGAATATTTAGACAACGTTATACAAAACCAAACAAAAATTCAATAAATAAATAAAAAAATTTTACTAAATTTTc                                         >  1:471452/1‑150 (MQ=255)
                                                                                                               tgCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGTCGGTTTTTTGTATCAGGAAGATGTTAAGAGGAATATTTAGACATCTTTATAACAATCCAAAAAAATATACATGAAAAATATATAAATATATTACTAATTGttatta                                      >  1:2651574/1‑150 (MQ=255)
                                                                                                               tgCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATAAGGAAGTTGTTAAGAGGAATATTTAGACATCGTTATACCAATCGAAAAATATATTCATGAAATATATATAAAAATTTTCCTAATTGTTCTTa                                      >  1:2651553/1‑150 (MQ=255)
                                                                                                                gCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTGATTGTTCTTAt                                     <  2:569900/150‑1 (MQ=255)
                                                                                                                gCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTAt                                     <  2:3554132/150‑1 (MQ=255)
                                                                                                                 cTGAGTTATGAATACATTGAAAACCGACAGCGGAAACGCTGGCGGTTTTTTGTATAAGGAAGATGTTCAGATGAATATTTAGACATAGTAATACCAAACGAAACATATATTCATGAAATATATATAAATAATTTCCTAATTGTTCTTATc                                    >  2:1941868/1‑150 (MQ=255)
                                                                                                                  tGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTTTATGAGGAAGATGTTCAGTGGAATATTTAGACAAAGGTATACCAAACGAAACATATATTCAAGAAATAAAAATTAATATTTTAAAAATTGTTATTATCt                                   >  1:1599205/1‑150 (MQ=255)
                                                                                                                  tGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCt                                   >  2:3155175/1‑150 (MQ=255)
                                                                                                                   gAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTg                                  >  2:4240575/1‑150 (MQ=255)
                                                                                                                     gTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGAc                                <  2:4519397/150‑1 (MQ=255)
                                                                                                                      ttttGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTTTATAAGGAAGATGTTAAGAGGAAAATTTATACAACGTTGTACCAATAGAAACAAATAATAAAGAAAAATATATAAATATTTTACTAATTTTtattatattaaa                               >  1:155666/1‑144 (MQ=255)
                                                                                                                       tttGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAg                              >  2:3245084/1‑150 (MQ=255)
                                                                                                                             tACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATAtct                        >  2:122540/1‑150 (MQ=255)
                                                                                                                                 ttGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACt                    >  2:1579864/1‑150 (MQ=255)
                                                                                                                                  tGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACtt                   >  2:1314495/1‑150 (MQ=255)
                                                                                                                                     aaaCCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTAAg                <  2:3662508/150‑1 (MQ=255)
                                                                                                                                             aGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTAAGGCTTTCtt        >  2:3990558/1‑150 (MQ=255)
                                                                                                                                              gCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTAAGGCTTTCTta       >  2:1892164/1‑150 (MQ=255)
                                                                                                                                               cGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTAAGGCTTTCTtat      <  2:4450713/150‑1 (MQ=255)
                                                                                                                                                ggAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTAAGGCTTTCTtata     <  2:4016431/150‑1 (MQ=255)
                                                                                                                                                ggAAACGCTGGCGGTTTTTTGTATCAGGAAGAGGTTCAGAGGTCTATTTAGTCTTCGTTATACCAATCGAAACATATATTCATGAATTATATATAAATATTTTCCTATTTGTTCTTATCGGACAGATATCTCACTTAAGGCTTTCTtata     <  2:3422200/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GGAAAGACCGCGCCCGCTTTTACAGCTACCTGCATCGCGTCTGGTCGAAAACCAGCGATAAACCGGTGTGGATGGATCAGGCCGAAAAGGCAACTGGTGATTTTTATTGATTGCTGAGTTTTGAATACATTGAAAACCGCCAGCGGAAACGCTGGCGGTTTTTTGTATCAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTAAGGCTTTCTTATAAAT  >  NC_000913/729231‑729527

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: