Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,381,918 A→C 5.2% intergenic (+16/‑29) ycjV → / → ompG pseudogene; putative ATP‑binding component of a transport system/outer membrane porin G

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,381,9180AC 5.2% 756.8 / 24.4 210intergenic (+16/‑29)ycjV/ompGpseudogene; putative ATP‑binding component of a transport system/outer membrane porin G
Reads supporting (aligned to +/- strand):  ref base A (115/84);  new base C (7/4);  total (122/88)
Fisher's exact test for biased strand distribution p-value = 7.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01

Only 100 of 215 total aligned reads displayed.

ACGGTTGGGGGCACGAGTTAGTGGTCCGTGCTGGTGCGTTAAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACTTTAATATCGGCGCGATGTACGAAATAGAAAACGTCG  >  NC_000913/1381769‑1382067
                                                                                                                                                     |                                                                                                                                                     
acGGTTGGGGGCACGAGTTAGTGGTCCGTGCTGGTGCGTTAAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCa                                                                                                                                                       >  1:1495120/1‑150 (MQ=255)
acGGTTGGGGGCACGAGTTAGTGGTCCGTGCTGGTGCGTTAAATGATTATCAGGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCa                                                                                                                                                       <  1:266266/150‑1 (MQ=255)
      gggggCACGAGTTAGTGGTCCGTGCTGGTGCGTTAAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTcccc                                                                                                                                                 >  2:2373304/1‑150 (MQ=255)
       ggggCACGAGTTAGTGGTCCGTGCTGGTGCGTTAAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTccccc                                                                                                                                                >  2:2185268/1‑150 (MQ=255)
       ggggCACGAGTTAGTGGTCCGTGCTGGTGCGTTAAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTccccc                                                                                                                                                >  1:3153734/1‑150 (MQ=255)
        gggCACGAGTTAGTGGTCCGTGCTGGTGCGTTAAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAATGCCTTccccct                                                                                                                                               <  2:4687739/150‑2 (MQ=255)
            aCGAGTTAGTGGTCCGTGCTGGTGCGTTAAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGat                                                                                                                                           >  2:61508/1‑150 (MQ=255)
             cGAGTTAGTGGTCCGTGCTGGTGCGTTAAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGata                                                                                                                                          <  2:408241/150‑1 (MQ=255)
             cGAGTTAGTGGTCCGTGCTGGTGCGTTAAATGATTAACATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGata                                                                                                                                          <  1:661643/150‑1 (MQ=255)
              gAGTTAGTGGTCCGTGCTGGTGCGTTAAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGataa                                                                                                                                         >  2:2264819/1‑150 (MQ=255)
                gTTAGTGGTCCGTGCTGGTGCGTTAAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGataata                                                                                                                                       <  1:450855/150‑1 (MQ=255)
                gTTAGTGGTCCGTGCTGGTGCGTTAAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGataata                                                                                                                                       <  1:358373/150‑1 (MQ=255)
                 ttAGTGGTCCGAGCTGGTGCGTTAAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGACATAGCAATTCTCTAAATACAGTGGTAAGGCATTACAACAGGCGAATAc                                                                                                                                      >  2:3100412/1‑150 (MQ=255)
                   aGTGGTCCGTGCTGGTGCGTTAAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACaa                                                                                                                                    <  1:520228/150‑1 (MQ=255)
                       gTCCGTGCTGGTGCGTTAAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAATGaa                                                                                                                                >  2:2245836/1‑150 (MQ=255)
                       gTCCGTGCTGGTGCGTTAAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGaa                                                                                                                                >  1:877413/1‑150 (MQ=255)
                       gTCCGTGCTGGTGCGTTAAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGaa                                                                                                                                >  2:697372/1‑150 (MQ=255)
                          cGTGCTGGTGCGTTAAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACaa                                                                                                                             >  1:2192134/1‑150 (MQ=255)
                              cTGGTGCGTTAAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACaataat                                                                                                                         >  1:1074917/1‑150 (MQ=255)
                                 gTGCGTTAAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGaa                                                                                                                      >  2:149850/1‑150 (MQ=255)
                                 gTGCGTTAAATGATTAGCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGaa                                                                                                                      >  2:1471505/1‑150 (MQ=255)
                                    cGTTAAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGaaaaa                                                                                                                   >  1:2451325/1‑150 (MQ=255)
                                       tAAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGtt                                                                                                                <  1:3423724/150‑1 (MQ=255)
                                       tAAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGtt                                                                                                                >  2:2844372/1‑150 (MQ=255)
                                           tGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGACGGCATTCCCCCAGGATAATACAAGGACCAATAATGACAAAGttattc                                                                                                            >  1:1706950/1‑149 (MQ=255)
                                              ttATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTAccc                                                                                                         <  1:3808440/150‑1 (MQ=255)
                                                  cATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTa                                                                                                     <  2:4801972/150‑1 (MQ=255)
                                                   aTGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTAc                                                                                                    <  2:4611417/150‑1 (MQ=255)
                                                    tGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTAcc                                                                                                   <  2:2720924/150‑1 (MQ=255)
                                                     gCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCg                                                                                                  <  1:601098/150‑1 (MQ=255)
                                                      cAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGc                                                                                                 >  1:2997012/1‑150 (MQ=255)
                                                         gagaAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACt                                                                                              >  1:3856981/1‑150 (MQ=255)
                                                          agaAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTg                                                                                             >  1:1690026/1‑150 (MQ=255)
                                                              aaTATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGa                                                                                         >  2:1203904/1‑150 (MQ=255)
                                                              aaTATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGa                                                                                         >  2:3893882/1‑150 (MQ=255)
                                                              aaTATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGa                                                                                         <  2:1878045/150‑1 (MQ=255)
                                                               atatCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGAt                                                                                        <  2:3512654/150‑1 (MQ=255)
                                                               atatCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGAt                                                                                        <  1:1606078/150‑1 (MQ=255)
                                                               atatCACGATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGAt                                                                                        <  1:3784034/150‑1 (MQ=255)
                                                                  tCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCAGTGGTGAtgtg                                                                                     >  2:2800930/1‑150 (MQ=255)
                                                                      tATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCg                                                                                 >  1:1375895/1‑150 (MQ=255)
                                                                      tATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCg                                                                                 >  1:4698732/1‑150 (MQ=255)
                                                                      tATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCg                                                                                 >  1:1372850/1‑150 (MQ=255)
                                                                      tATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCg                                                                                 <  1:3480267/150‑1 (MQ=255)
                                                                        ttCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCggg                                                                               >  1:1838167/1‑150 (MQ=255)
                                                                          cATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGaa                                                                             >  2:4525928/1‑150 (MQ=255)
                                                                           aTTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAAt                                                                            >  2:4230591/1‑150 (MQ=255)
                                                                            ttttGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATg                                                                           >  2:3174458/1‑150 (MQ=255)
                                                                              ttGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGc                                                                         <  1:2857555/150‑1 (MQ=255)
                                                                                gATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCt                                                                       <  1:580469/150‑1 (MQ=255)
                                                                                   atGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTgcg                                                                    <  1:1972740/150‑1 (MQ=255)
                                                                                     gACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGca                                                                  <  2:387680/150‑1 (MQ=255)
                                                                                       cGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGGAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGcaca                                                                <  2:3827094/150‑1 (MQ=255)
                                                                                        gAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAg                                                               >  2:816022/1‑150 (MQ=255)
                                                                                        gAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAg                                                               >  1:4288119/1‑150 (MQ=255)
                                                                                        gAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAg                                                               >  1:4668454/1‑150 (MQ=255)
                                                                                         aaaTGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAgg                                                              <  2:3573829/150‑1 (MQ=255)
                                                                                           aTGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGcc                                                            <  1:770742/150‑1 (MQ=255)
                                                                                             gTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGTGCGCAGGccat                                                          <  2:1587465/150‑3 (MQ=255)
                                                                                             gTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGa                                                          <  1:1666059/150‑1 (MQ=255)
                                                                                             gTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGa                                                          <  1:2717984/150‑1 (MQ=255)
                                                                                             gTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGa                                                          <  2:2674808/150‑1 (MQ=255)
                                                                                               cATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGTGCGCAGGccattc                                                        <  1:2737105/150‑5 (MQ=255)
                                                                                                aTTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGagga                                                       >  2:4370400/1‑150 (MQ=255)
                                                                                                aTTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGagga                                                       >  2:2516935/1‑150 (MQ=255)
                                                                                                     tttGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGaggaaagga                                                  <  1:4751837/150‑1 (MQ=255)
                                                                                                     tttGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGGGATGTGTGCGGGAATGGCCTGCGCACAGGCCGaggacagga                                                  >  1:1721514/1‑150 (MQ=255)
                                                                                                      ttGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGACTGGCCTGCGCACAGGCCGaggaaaggaa                                                 >  2:2111241/1‑150 (MQ=255)
                                                                                                      ttGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGaggaaaggac                                                 >  2:486316/1‑149 (MQ=255)
                                                                                                        gATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACg                                               >  2:438545/1‑150 (MQ=255)
                                                                                                         aTGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGa                                              >  1:3098398/1‑150 (MQ=255)
                                                                                                         aTGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGa                                              >  1:2496893/1‑150 (MQ=255)
                                                                                                           gCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACt                                            >  1:4450374/1‑150 (MQ=255)
                                                                                                           gCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACt                                            >  1:2576110/1‑150 (MQ=255)
                                                                                                            cAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTg                                           >  2:2631057/1‑150 (MQ=255)
                                                                                                            cAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTg                                           >  2:1690905/1‑150 (MQ=255)
                                                                                                             aGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTgg                                          >  1:2828961/1‑150 (MQ=255)
                                                                                                               aaaCGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCa                                        <  2:1997209/150‑1 (MQ=255)
                                                                                                                 aCGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACt                                      >  1:690845/1‑150 (MQ=255)
                                                                                                                 aCGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACt                                      >  2:401163/1‑150 (MQ=255)
                                                                                                                 aCGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGGGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACt                                      >  2:4403598/1‑150 (MQ=255)
                                                                                                                  cGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACtt                                     <  2:1618238/150‑1 (MQ=255)
                                                                                                                   ggAAATAGCAATTCGCTAAAGACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACAACTGGCACttt                                    <  2:3321571/150‑1 (MQ=255)
                                                                                                                       aTAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACTTTAata                                >  2:3729506/1‑150 (MQ=255)
                                                                                                                          tcAATTCGCTAAATACAGGGGTAAGGCATTCCCCCAGGATAATACAAGCAACAATAATTAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAAGGGCCTGCGCACAGGCCGCGGAAAGCAACGACTGGCACTTTAATATCg                             <  1:3640572/149‑1 (MQ=255)
                                                                                                                          caaaTTCGATAAATACCGGGGTAAGCCATACCCCCTGTATAATACACGCAAAAATAATGAAAATGTTATTACCGTGTACCGCAGTGGTGATGTGTGGGGGATTGGCCGGCGCACTGGCGTTGTAAAGTAACGACTGGCACTTTAATATCg                             <  1:1237203/148‑1 (MQ=255)
                                                                                                                           cAATTCGATAAATACTGGGGGATGGCATTCCCCCTGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGTTGTGTGGGGGAATGGCCTGCGCACCGGCCGTGGAAAGGAACGACTGGCACTTTAATATCgg                            <  1:1778577/150‑1 (MQ=255)
                                                                                                                              ttCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACTTTAATATCGgcgc                         >  2:1892418/1‑150 (MQ=255)
                                                                                                                                cGCTAAATACTGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACTTTAATATCGGCGCGa                       <  1:4314498/150‑1 (MQ=255)
                                                                                                                                cGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACTTTAATATCGGCGCGa                       >  1:2392132/1‑150 (MQ=255)
                                                                                                                                cGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACTTTAATATCGGCGCGa                       >  1:3567589/1‑150 (MQ=255)
                                                                                                                                        cctgggggAATGCCTTCCCCCTGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACTTTAATATCGGCGCGATGTACGaa               >  2:4180076/4‑150 (MQ=255)
                                                                                                                                        cctgggggAATGCCTTCCCCCTGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACTTTAATATCGGCGCGATGTACGaa               >  1:278751/4‑150 (MQ=255)
                                                                                                                                        cctgggggAATGCCTTCCCCCTGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACTTTAATATCGGCGCGATGTACGaa               >  1:3575038/4‑150 (MQ=255)
                                                                                                                                         ctgggggAATGCCTTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACTTTAATATCGGCGCGATGTACGaaa              >  1:1184472/3‑150 (MQ=255)
                                                                                                                                          tgggggAATGCCTTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACGGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACTTTAATATCGGCGCGATGTACGAAAt             >  1:3899098/2‑150 (MQ=255)
                                                                                                                                           gggggAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACTTTAATATCGGCGCGATGTACGAAATa            <  2:1304796/150‑1 (MQ=255)
                                                                                                                                              ggAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACTTTAATATCGGCGCGATGTACGAAATAGaa         >  1:822333/1‑150 (MQ=255)
                                                                                                                                               gAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGTCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACTTTAATATCGGCGCGATGTACGAAATAGaaa        >  1:660542/1‑150 (MQ=255)
                                                                                                                                                aaGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACTTTAATATCGGCGCGATGTACGAAATAGaaaa       <  2:2409188/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ACGGTTGGGGGCACGAGTTAGTGGTCCGTGCTGGTGCGTTAAATGATTATCATGCAGGAGAAAATATCACTATTCATTTTGATATGACGAAATGTCATTTCTTTGATGCAGAAACGGAAATAGCAATTCGCTAAATACAGGGGGAAGGCATTCCCCCAGGATAATACAAGGAACAATAATGAAAAAGTTATTACCCTGTACCGCACTGGTGATGTGTGCGGGAATGGCCTGCGCACAGGCCGAGGAAAGGAACGACTGGCACTTTAATATCGGCGCGATGTACGAAATAGAAAACGTCG  >  NC_000913/1381769‑1382067

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: