Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 164,581 | A→C | 20.9% | intergenic (+47/‑149) | hrpB → / → mrcB | putative ATP‑dependent helicase/fused glycosyl transferase and transpeptidase |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 164,581 | 0 | A | C | 20.9% | 180.2 / 26.6 | 130 | intergenic (+47/‑149) | hrpB/mrcB | putative ATP‑dependent helicase/fused glycosyl transferase and transpeptidase |
Reads supporting (aligned to +/- strand): ref base A (27/74); new base C (6/23); total (33/97) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 6.31e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
Only 100 of 133 total aligned reads displayed.
CCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGC > NC_000913/164432‑164729 | ccGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCa > 2:3683047/1‑150 (MQ=255) cGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGAAt > 1:889766/1‑150 (MQ=255) tGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCa > 2:1373834/1‑150 (MQ=255) tGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCa > 1:3158360/1‑150 (MQ=255) aaaaGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCCTCAGCGCGCACg > 1:4549101/1‑150 (MQ=255) aaaGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGt > 2:1957046/1‑150 (MQ=255) aaGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGtg > 1:3834234/1‑149 (MQ=255) agagATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTtc > 2:2787680/1‑150 (MQ=255) agagATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGtgg > 2:1799984/1‑148 (MQ=255) agagATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGtgc > 1:3571517/1‑148 (MQ=255) cgatgatTGGGCGTTAACCCAAACATGTCGGGCGGGACGACCCGGAAAATACTGCACCGCCGCGAGGCACTAAAATGTTTTCTTATGGTTGG‑TTTTCTCCCGCTCCGTGGGGT‑TGTGGGCGGGGGTGTGGGCATCAGCGCGCACGTtcac < 2:1883129/149‑1 (MQ=255) ttttaatgggCTTTATCCCAAAAATGTCGGGCCTTACGACCGGCAAAATATGCCACCGACGCGAGGGACGAAAATTTATTCGTTTGTTTGT‑TTTTCTCCCTCTCCGTGTGGG‑TGTGGGCGGGGGTGTGGGCATCAGCGCGCACGTtcacc < 2:114620/143‑1 (MQ=255) tatcaaaGGGCTTTAACCCAAACATTACTGGCCGCACCACCCGCAAAAAACTCCACCCACGCGACGCACGAAAAATTATTCCTAAGTTTGT‑TTTTCTCCCACACCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcacc < 2:1994936/146‑1 (MQ=19) gaTGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCAGCCGCGCGCACGtgcccc > 2:4102342/1‑145 (MQ=255) ggCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtgacccccaaccc > 2:87242/1‑149 (MQ=255) cGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCCGCGCGCACGTGCACCCACACCCCaa > 2:646505/1‑150 (MQ=255) ttATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTTTTCGTATGGTTGG‑TTTTCTCCCTCTCCCTGTGGG‑TGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAAcc < 2:720442/150‑1 (MQ=255) tATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGGCCCGCACCCAAAacc > 1:771054/1‑150 (MQ=38) aTCCCAAACATGTCTGGCCGGACGACCCGGAAAATACTGCACGGACGCGACGGACGAAAATGTATTCGTATGGTTGG‑TTTTCTCCCTCTCCCTGTGGG‑TGAGGGCCGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCct < 2:3898376/150‑1 (MQ=38) tCCCAACCATGTCTGGCAGGAGTACGCGTAATTTAGTGCACAGTCGCGACTGACGAAATTGTATTCGTATGGTTGT‑TTTTCTCGCTATCACTGTGGT‑AGTGGGCAGGGGTGTGTGAATCAGCGCGCACGTTCACCCTCACCCTaaaactc < 2:3568887/150‑6 (MQ=255) tCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCctc < 2:3568958/150‑1 (MQ=255) cccAACCATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACTAAAAAGTATTCGTACGGTTGG‑TTTTCTCCCTCTCCCTGTGGG‑TGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCctct < 2:564081/150‑1 (MQ=255) cccAAACTTGTGGGGCCGGACGACCCGCAAAACACGGCCCCGACGCGACGGACGAAAATGTATTCGTATGGTTGG‑TTTTCCCCCCCTCCCTGTGGG‑AGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCctct < 1:3987728/150‑1 (MQ=37) ccAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGACCCCCACCCAAAcccacac > 1:2308812/1‑146 (MQ=38) cAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTGCCCCCCCCCCCCCAcccacacc > 1:3362530/1‑145 (MQ=37) ggccggcccGGACGCCCCGCCAACTCCGGCACCGCCCCGACGGACCACCAAGTATTCGCACGGTGGG‑TTGGCGCCCGCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCCGCGCGCCCGTTCACCCTCACCCTAACCCTCTCCCTCAAg < 2:2739614/143‑1 (MQ=255) ggTCGGGCCGGACCCCCCGCCAAATACGGCACCGAGGCGCCGCACGAAAATTTATTGGTATGGTTGT‑TTTTCGCCCACCCCGGGGGGT‑TGTGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAg < 2:1729422/149‑1 (MQ=255) tCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGCCCCCCCCCCCGAAACCACTcccgcccggg > 1:277500/1‑143 (MQ=37) gccggccgCCCCGGAAAATCCTCCACCGACGCGACGGTCTAAAATTTTTTGTTATGTTTGG‑TTTTCTCCCTCGGCGTGTGGG‑TGCGGGCGGGGGTGAGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGagag < 1:2634351/150‑1 (MQ=255) gCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCACCCCCAAACCCCCTCCCACCAggggggg > 2:3926941/1‑146 (MQ=37) gCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCCCGTGCCCCCGCACCCCAACCCACACCCCCAAggggggg > 2:2954720/1‑146 (MQ=37) ccGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGGCCCCCACCCAAACCCACACCCACAACGGGGAgg > 2:4101359/1‑150 (MQ=37) cGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGGGCACCCCCACCCAAAACCACACCCACCAGGGAGgggg > 1:1480559/1‑150 (MQ=37) cGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAggg < 2:4723510/150‑1 (MQ=255) cGGACG‑CCCGGCAAAATCCGCCCCGCCGGGGCGGCCCTAAAAATTTTGCTTAGGTTGGTTTTTCACCCACCCCGTGGGGT‑CGGGGGCGGGGGTGCGGCCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTTGAGGGAGAggg < 2:1272845/150‑1 (MQ=255) ggACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGCGCCACACCCAAAACCACAACCACAAGGGCGAgggg > 1:1426749/1‑150 (MQ=25) ccGCCAAATCCGGCCCCGCCGCGACGGACGAAAACGTTTTCGTAAGGTTGG‑TTTTCTCCCCCCCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATc < 2:2521740/150‑1 (MQ=37) ggCATAAACGGCACCGTCGCGCCTGACGAAATTGTATTCGTACGTTGTT‑TTTTCACCTTCTCGAGGGGTG‑AGTGGGCGGGGTTGAGGGCATCAGCGCGCAGGTTCACCCTCACCCTAACCCTCTCCCTCAATGGAGAGGGGACCGTTCGa < 2:446001/150‑1 (MQ=255) gccAAATCCTGCCCCGACGCGCCGGACCAAAATGTATCCTCACGTTTGG‑TTGACCCCCTCCCCGTGGGGG‑TGTGGGCGGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGa < 2:4059325/148‑1 (MQ=255) gcaaaaTACTGCCCCGACGGGCGGGACTAAAATGTTTTCGTATGTTTGG‑TTTTCGCCGTGTCCCTGTGGG‑TGCGGGCGGGGGTGCGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGa < 2:4058975/147‑1 (MQ=255) gCAAATACTGCCCCGCCGCGCCGGACGAAAACTTATTCGTATGGTTGG‑TTTTCACCCTCTCCCTGTGGG‑TGAGGGCCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAg < 2:2512286/150‑1 (MQ=255) gCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGGGGGCCCCCACCCGAAACCACGCCCTCAAGGGGGAGGGGGCCGGTCGAg > 1:746039/1‑150 (MQ=25) ccAAAGACTGCCCCGACGGGCCGGACCAAAAAGTATTCGTACGGTGGG‑TTTTCGCCCTCTCCCTGGGGG‑AGTGGGCCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAg < 1:2755647/149‑1 (MQ=255) cAAATCCTCCACCGAGGCGCCGGACCAAAAAGTATTCGAACGTTTGT‑TTTACCCCCACTCACTGGGGG‑AGCGGGCGGGGGTTTGGGCATCAGCGCGCCCGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGc < 1:342522/150‑1 (MQ=255) aaaTCCGGCACCGCGGCGCCGGTTGAAAATGTTTTGGTCTGGTTGG‑TTTTCGCCCTCGCCGGGGGGG‑AGTGGGCGGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGca < 1:3458360/150‑1 (MQ=255) cACCGCCGCTACGCACAAAAAATTATTCTTATGGTTTT‑TTTTCCCCCTCTCCGTGTGTT‑AGCGGGCGGGGGTGAGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAAtttt < 1:3262724/150‑1 (MQ=255) ccgccgCGACGGCCCAAAATTTTTTCTTATGGTTGG‑TTTTCCCCCCCCCCGTGGGGG‑TGTGGGGGGGGGTGCGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTga < 1:1469459/150‑1 (MQ=255) ccGACGCGCCGGACCAAAATTTATTCGTACGTTTGG‑TTTTCTCCCACTCCCTGTGGT‑TGCGGGCGGGGTTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTga < 2:3834234/150‑1 (MQ=255) cgacgcgacgTCCTATATATTATTCTTAGGCTTGG‑TTTTCGCCCGCGCCCTTTGGT‑TGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTgag < 2:1473763/150‑1 (MQ=255) gacgcgacgGACTAAAATGTATTCGTATGGTTGG‑TTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTgaga < 2:777288/150‑1 (MQ=255) acgcgacgGACGAAAAAGTATTCGTAAGGTTGG‑TTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGCACCCACACCCCAAACCCCACCCACACCGGGGAGGGGGCCGCGACAGCACCAAATTTgagtg > 2:3052020/1‑148 (MQ=21) gtaggAAAAAGTATTCGTAAGGTTGG‑TTTTCTCCCTCTCCCGGTGGG‑AGTGGGCGGGGGTTAGGGCCTCAGCGCGCACGTTCACCCTCACGCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGTGATAtctt < 2:2737265/146‑1 (MQ=255) gAAAAAGTATTCGTAAGGTTGG‑TTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCCCAAGGGAGAGGGGACCGTTCGAGCACAAATTTTGAGAGATAtcttcttc < 2:3912934/150‑1 (MQ=255) gAAAAAGTATTCGTAAGGTTGG‑TTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCACACACGAAAACACTAACGCCAGGGAGAGGGGGCCCATCGAGCACAAAATTTGTGAGAAAtattctta > 2:747288/1‑149 (MQ=21) aaataGTATCCGTTCTGTTGT‑TTTTCCCCCTCACCCTTTGGG‑TGAGGGCCGGGGTGTGGGCATCAGCGCGCACGTTCACCCTAACCCTAACCCTATCCCTCAAGGGAGAGGGGACCGCTCGAGCACAAATTTTGAGAGATAtcttcttat < 2:1077360/150‑3 (MQ=255) aaaaaGTATTCGTACGGTTGG‑TTTACTCCCTCACCATGTGGG‑AGAGGGCCGGGGTGAGGGCATCCGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtcttcttct < 2:1075304/150‑1 (MQ=255) aaGTATTCGTAAGGTTGG‑TTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCAACCGCACCCAAAACCACTACCACAACGGGGAGGGGGCCCATCGAGCACAAAATTTGTGAGATATATTATTCTGtg > 2:4284166/1‑149 (MQ=21) ttATTCGTAAGGTTGG‑TCTGCCCCCCCCCCCGGTGGG‑TGTGGGCGGGGGTGCGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCtt < 1:428416/149‑1 (MQ=255) gTATTCGTATGGTTGT‑TTTTCTCCATCTCCCTGTGGG‑ATTGGGCCGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACTAATTTTGAGAGATATCTTCTTCTGTCtt < 2:380627/150‑1 (MQ=255) gTATTCGTAAGGTTGG‑TTTTCTCCCTCTCCCTGTGGG‑TGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCtt < 2:2819206/150‑1 (MQ=255) ttttggTATGGTTGG‑TTTTCTCCCTCTCCCTGTGGG‑TGAGGGCCGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTg < 2:2370489/145‑1 (MQ=255) ttttCGTATGTTTGG‑TTTTCTCCCTCTCCCGGGGGG‑AGTGGGCGGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTg < 1:923438/148‑1 (MQ=255) tttgcGTAAGGTTTG‑TTTTCTCCCTCTCCCTTTGGG‑TGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTg < 2:2292383/146‑1 (MQ=39) tATTCGTTTGGTTGT‑TTTTCTCCCGCTCCGTGGGGG‑AGTGGGCGGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTg < 1:923419/150‑1 (MQ=255) tggTATGGTTGG‑TTTCCGCCCCCGCCCTGGGGG‑TGCGGGCGGGGGTGGGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTaa < 2:1816158/148‑1 (MQ=255) tCGTATGGTTGG‑TTTTCTCCCTCGCCCGGTGGG‑TGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTaa < 2:4296628/150‑1 (MQ=39) gTAAGGTTGG‑TTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACa < 2:3158360/150‑1 (MQ=255) taGGTTGT‑TTTTCTCCCTCCCCGTGTGGG‑TGAGGGCCGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACaga < 1:2637701/149‑1 (MQ=255) atgtttgg‑ttTTCGCCCTCCCCCTGTGGT‑TGTGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACaga < 1:2637504/146‑1 (MQ=255) aaGGTTGG‑TTTTCTCCCTCTCCCTGTGGG‑AGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACaga < 2:363033/150‑1 (MQ=255) ttgg‑ttTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCGCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAgaagaa < 2:3049672/150‑1 (MQ=255) tgg‑ttTTCTCCCTCTCCCTGTGGG‑AGCGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAAc < 1:756927/150‑1 (MQ=255) tgg‑ttTACTCCCTCTCCCTGGGGG‑CGTGGGCCGGGTTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAAc < 1:2297629/150‑1 (MQ=255) tgtttttCTCCCTCTCCCTGGGGG‑AGAGGGCGGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAAc < 2:1867597/147‑1 (MQ=255) g‑ttTTCCCCCCCCCCCGGGGGGTAGTGGGCGGGGGTGCGGCCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGACGAACa < 1:1281904/150‑1 (MQ=255) ttttCTCCCTCTCCCTGTGGG‑AGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGa < 1:1174195/150‑1 (MQ=255) ttttCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGa < 1:1174443/150‑1 (MQ=255) ctccctctccctGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAAt < 2:2028665/150‑1 (MQ=255) tccctctccctGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATc < 2:3125609/150‑1 (MQ=255) tccctctcccgGTGGG‑AGAGGGCGGGGGTGAGGGCATCCGCGCGCACGTTCACCCTCACCCTAACCCTCTCACTCAAGGGAGAGGGGACCGATCGAGCCCAAATTTTGAGAGATATCTTCTTCTGTATTGTAACAGAAGAACAGAATATc < 2:2394215/150‑1 (MQ=255) tgGG‑TGTGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTgcgc < 2:4174898/150‑1 (MQ=255) gggg‑AGTGGGCGGGGGTGCGGGCCTCCGCGCGCACGTTCACCCTCACCCTTCCCCTCTCCCTCAAGGGAGAGGGGACCGATCGCGCACAAATTTTGAGAGATATCTTCTTCTGCCTTGTAACAGAAGAACAGAAAATCGGGCTTTTgcgc < 2:1879098/149‑1 (MQ=255) gggg‑AGTGGGCCGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTgcgc < 2:4174916/149‑1 (MQ=255) ccGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTg < 2:3140162/150‑1 (MQ=255) ccGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTg < 2:3140408/150‑1 (MQ=255) ggggggAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCg < 2:812043/150‑1 (MQ=255) ggggTGCGGGCCTCCGCGCGCCCGGTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCg < 1:4548320/150‑1 (MQ=255) ggggTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTACCCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCg < 1:4296447/150‑1 (MQ=255) ggggTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTACCCCTCTCCCTCAAGGGAGAGGGGACCGAGCGAGCCCAAATTTTGAGAGATATCTTCTTCGGTCTTGTAACAGAAGAACAGAAACTCGGGCTTTTGCGCCTGAATATTGCg < 2:2246729/150‑1 (MQ=255) ggggTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCg < 2:2928212/150‑1 (MQ=255) ggggTGCGGCCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCg < 1:1799984/150‑1 (MQ=255) ggggTGAGGGCCTCAGCGCGCCCGTGCACCCTCACCCGAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCg < 1:4296388/150‑1 (MQ=255) ggggTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCg < 1:2787680/150‑1 (MQ=255) ggggTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCg < 1:4599114/150‑1 (MQ=255) ggggTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCg < 1:1521026/150‑1 (MQ=255) ggggTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCg < 2:3571517/150‑1 (MQ=255) ggggTGAGGCCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTACTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCg < 1:3052020/150‑1 (MQ=255) gAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGaa < 2:3827117/150‑1 (MQ=255) ggCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGaaaaa < 2:679432/150‑1 (MQ=255) cATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGc < 2:3701742/150‑1 (MQ=255) | CCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGC > NC_000913/164432‑164729 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |