Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,177,245 G→T 15.9% intergenic (‑41/+267) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,177,2450GT15.9% 538.5 / 20.8 251intergenic (‑41/+267)gatY/fbaBD‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
Reads supporting (aligned to +/- strand):  ref base G (108/91);  new base T (32/18);  total (140/111)
Fisher's exact test for biased strand distribution p-value = 2.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 253 total aligned reads displayed.

TTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATAC  >  NC_000913/2177097‑2177394
                                                                                                                                                    |                                                                                                                                                     
ttCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGa                                                                                                                                                      <  2:2466269/150‑1 (MQ=255)
   tACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGATTTAAAAATATAATTAAat                                                                                                                                                   >  1:152882/1‑150 (MQ=255)
   tACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAATGTAat                                                                                                                                                   >  1:1294181/1‑150 (MQ=255)
   tACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTCAAAAAATAATGAAat                                                                                                                                                   >  2:2988751/1‑150 (MQ=255)
    accaccACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAatt                                                                                                                                                  >  2:1558026/1‑150 (MQ=255)
    accaccACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAATTAAatt                                                                                                                                                  >  1:157887/1‑150 (MQ=255)
     ccaccaCTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATCCCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATGTCAAAATATAATGAAatta                                                                                                                                                 >  2:1580575/1‑150 (MQ=255)
     ccaccaCTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAatta                                                                                                                                                 >  2:3047221/1‑150 (MQ=255)
      caccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAattat                                                                                                                                                >  1:3115043/1‑150 (MQ=255)
      caccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAattat                                                                                                                                                <  1:3481521/150‑1 (MQ=255)
      caccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTCAAAATATAATGAAattat                                                                                                                                                >  2:3199317/1‑150 (MQ=255)
         cacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTg                                                                                                                                             >  2:3993795/1‑150 (MQ=255)
         cacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACAGCAGAGATGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGACGTTTTTTTTAGATTTAAAAATATAAAGAaattaattt                                                                                                                                             >  2:1061714/1‑149 (MQ=255)
           cTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGtt                                                                                                                                           <  1:713380/150‑1 (MQ=255)
            ttGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGttt                                                                                                                                          <  1:2387792/150‑1 (MQ=255)
            ttGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGttt                                                                                                                                          <  1:581724/150‑1 (MQ=255)
               cATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTa                                                                                                                                       <  2:242084/150‑1 (MQ=255)
                 tCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTaaa                                                                                                                                     >  2:2741225/1‑150 (MQ=255)
                 tCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAAAAAAATTAAATTATTTTTTTTTtaa                                                                                                                                     >  1:835629/1‑150 (MQ=255)
                    tttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAATATAATTTAAATATTTTTTTTTAAatat                                                                                                                                  >  1:3431083/1‑150 (MQ=255)
                     ttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATc                                                                                                                                 <  2:866527/150‑1 (MQ=255)
                     ttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATc                                                                                                                                 <  1:496999/150‑1 (MQ=255)
                     ttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATc                                                                                                                                 <  1:4270941/150‑1 (MQ=255)
                     ttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATc                                                                                                                                 <  2:444651/150‑1 (MQ=255)
                        gagaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGATTTAAAAATATAATTTAAATATTTTTTTTTAAAAATAgat                                                                                                                              >  1:1332902/1‑149 (MQ=255)
                        gagaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGATTTAAAAAAAAAAATAAAAAAATTTTTTTTAAAAATagag                                                                                                                              >  2:2206027/1‑150 (MQ=255)
                          gaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGAt                                                                                                                            <  1:4356256/150‑1 (MQ=255)
                           aTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTCGATTTCAAAATATAATGAAATTAGTTTTTTTTAAATATCGAGATa                                                                                                                           >  1:4037090/1‑150 (MQ=255)
                              gtgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAAAATAGAGATaaag                                                                                                                        >  2:1359211/1‑148 (MQ=255)
                              gtgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAAATAGTTGTTTTTAAATATCGAGTTAACg                                                                                                                        >  2:3170571/1‑150 (MQ=255)
                               tgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGa                                                                                                                       <  1:2894914/150‑1 (MQ=255)
                               tgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGa                                                                                                                       <  1:4440554/150‑1 (MQ=255)
                                        aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATAGAGATAACGATCACaaaaa                                                                                                              >  1:3469807/1‑150 (MQ=255)
                                        aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAATGAAATTATTTTTTTTTAAATATAGAGATAAAGATaaaaaaaa                                                                                                              >  2:1652066/1‑150 (MQ=255)
                                        aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCCATTTCCAAATAAAAAGAAAATATTTGTGTGTAAATATCGAGATAACCATCACaaaaa                                                                                                              >  2:4664875/1‑150 (MQ=255)
                                        aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACAGTTTCATATCCTGTCGTTTGTTTTGGAATTAAAAAAATATTTAAATTATTTGTTTTTAAATATTGTGATTAACATCACaaaaa                                                                                                              >  1:1902005/1‑150 (MQ=255)
                                        aaTGCCGGAACCGCATAACCGCCGCGCGGTGCGTTGTTCAGCATCTGCTTTGTCGAGACCACCTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACaaaaa                                                                                                              <  1:4695029/150‑1 (MQ=255)
                                         aTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAAc                                                                                                             >  2:4140128/1‑150 (MQ=255)
                                           gctggAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGa                                                                                                           <  2:1904582/147‑1 (MQ=255)
                                           gCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGa                                                                                                           <  2:4287179/150‑1 (MQ=255)
                                           gCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTGTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAATGAAATTATTTTTTTTTAAATATAGATATAACGATCAAAAAAAAGa                                                                                                           >  1:1991729/1‑150 (MQ=255)
                                             cGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAATATAATGTAATTATTTTTTTTTAAATATAGAGATAAAGATAACAAAAAAGaaa                                                                                                         >  1:3843770/1‑148 (MQ=255)
                                               gAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAATATAATTAAATTATTTGTTTTTAAAAATAGAGATAACTATAACAAAAACGACaaa                                                                                                       >  2:2571245/1‑149 (MQ=255)
                                                   cGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTGGATTTCAAAAAATAATGAAAATATTTTTTTTTTAATATAGAGATAAACATGAAAAAAAAAACActattt                                                                                                   >  1:10047/1‑148 (MQ=255)
                                                   cGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGAATTGAAAATATAAATAAAATATTTGTTTTTTAATATAGAGATAACGATAACAAAAAAGAccaaatta                                                                                                   >  2:1712843/1‑143 (MQ=255)
                                                   cGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGGTTTGAAAAAATAATGAAATTATTTTTTTTTTAAAATAATTAAAAAGATCACAAAAACAAAAAAATGa                                                                                                   >  1:4436418/1‑150 (MQ=255)
                                                    gaaTAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGACGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACTATCACAAAAACGACAATATGaa                                                                                                  <  2:4602474/148‑1 (MQ=255)
                                                    gCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTTTATTTAAAAATATAATTAAATTATTTTTTTTTTAATTTAGTGATAAAGAAGAGAAAAAAGAAAATATgtt                                                                                                  >  1:2231041/1‑148 (MQ=255)
                                                    gCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTTGATTTAAAAATATAATGGAAATATTTTTTTTTTAAAATAGAGGTAACGATCACAAAAAAGACCAGATgga                                                                                                  >  1:2215944/1‑148 (MQ=255)
                                                     cATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGaaa                                                                                                 <  2:1169188/150‑1 (MQ=255)
                                                     cATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAAAGATAAAAAAAAAGACAATATTaaa                                                                                                 >  2:2601038/1‑150 (MQ=255)
                                                         aCCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAACACGACAATATGAAAatta                                                                                             <  2:1282594/150‑1 (MQ=255)
                                                         aCCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAatta                                                                                             <  2:2294320/150‑1 (MQ=255)
                                                               gcgcTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCgag                                                                                       <  2:2732216/150‑1 (MQ=255)
                                                               gcgcTGTGCGTTGTACAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCgag                                                                                       <  2:148776/150‑1 (MQ=255)
                                                                     tgCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATATCAAAATATAATGAAATTATTTGTTTTTAAATATAGAGATAAAGATCACAAAAAAGACAATATGAAAATTATTCGAGGAGTGa                                                                                 >  2:1901266/1‑150 (MQ=255)
                                                                             tcatcaTCTGATTTGTCGATACCACGTACATTTTCATATCCTGTCTTTTGTTTTCGATTTAAAAATATAATTAATTTTTTTTTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaaa                                                                         <  1:2621023/150‑1 (MQ=255)
                                                                             tCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAAATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaaa                                                                         >  2:4404906/1‑150 (MQ=255)
                                                                             tCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAAAATAATTAAAATATTTTTTTTTAAATAAAGAGATAAAGATCACAAAAACCAAAAAAAGAAAAATATTAGAGGAGTGGAAGGCaaa                                                                         >  2:35085/1‑150 (MQ=255)
                                                                             tCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTCAAAATATAAAGAAATTATTTTTTTTTAAATATCGAGATAAAGATCACAAAAACGACAAAATGAAAAATATTCGAGGAGTGAAACGCaaa                                                                         >  2:4219317/1‑150 (MQ=255)
                                                                                 cATCTGTTTTGTCGATACCACTTACATTTTCATATCCGTTCTTTTTTTTTATATTACAAAATATAATTAATTTATTTTTTTTTAAATATCGAGAAAACTATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaaaaaaa                                                                     <  1:1149833/150‑1 (MQ=255)
                                                                                     tGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTTGTTTTTAAAAAAAAAATAAAATATTTTTTTTTTAATAAGTTGATAACGATGAAAAAAAAGACAAAATTTAAATTATTATAGGAGGGTTAGGCAAAAAAAAGGc                                                                 >  1:638013/1‑150 (MQ=255)
                                                                                     tGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTATATTTTAAAAAAAAAAGTAAAAATATTTTTTTTTATATAGATATAAACATAAAAAAAAAAAAAAAAATAAAAATATTAGAGGAGTTGAAGGCaaaaaaaaaac                                                                 >  1:982411/1‑146 (MQ=255)
                                                                                       cTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAAATAATTTTTTTTAAATATCGAGATAACGATAACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAAGGcca                                                               >  2:238990/1‑149 (MQ=255)
                                                                                         ttGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTcc                                                             <  2:3414468/150‑1 (MQ=255)
                                                                                         ttGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAATGAAAATATTTGTTTTTAAATATAGAGATAACGATCACAAAAACGACAAAATGAAAATTATTAGAGGAGTGAAAGGCAAAAAAACGGCCTcc                                                             >  2:4764839/1‑150 (MQ=255)
                                                                                            tCGATACCACGTAAATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATCCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGa                                                          <  2:4000852/150‑1 (MQ=255)
                                                                                                tcccccGTACATTTTCATATCCGGTCGTTTGTTTTCGATTTCAAAATATAAGGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAgg                                                      <  2:486292/145‑1 (MQ=255)
                                                                                                  ccACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAAATAATGAAATTATTTGTTTTTAAATATCGCGATAACGATCCCAAAAACGACAAAATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAAGGCCGCCCGCTAgggc                                                    >  2:2915689/1‑149 (MQ=255)
                                                                                                   cACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACGATGACAAAAACGACAATATGAAGATTATTGGTGGTGTGAAAGGGAAAAAAAAGGGCTCCCGATAgggga                                                   >  2:2638387/1‑148 (MQ=255)
                                                                                                     cttaatttttCATCTCCTTTCTTTTGTTTTCTTTTAAAAAATTTATTTAAATTATTTTTTTTTAAATATCTATATAACTATCAAAAAAACGACAATATTAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGc                                                 <  1:2455801/144‑1 (MQ=255)
                                                                                                         cATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTa                                             <  2:2958805/150‑1 (MQ=255)
                                                                                                          aTTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATTAAAATTATTCGAGGAGTGAAAGGCAAAAAAAAGGCCTCCCGATAGGGAAGCCGTAg                                            >  2:2697294/1‑150 (MQ=255)
                                                                                                          aTTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAg                                            <  2:1476334/150‑1 (MQ=255)
                                                                                                          aTTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAAGGCCTCCCGATAGGGAAGCCGTAg                                            >  2:2697418/1‑150 (MQ=255)
                                                                                                              tCAAATCCTGTCTTTTTTTTTCTATTTCAAAATATAATTAAATTTTTTTTTTTTAAATATCTATATAACTATAACAAAAACGACAATATTAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCTTCCCTATCGGGAGGCCGttttttt                                        <  1:896622/150‑7 (MQ=255)
                                                                                                                   tCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTgcg                                   <  2:462996/150‑1 (MQ=255)
                                                                                                                    ccTGTGTTTTTTTTTCTATTTAAAAATATAATTAATTTTTTTTTTTTTAAATATCTATATAACTATAAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTgcgc                                  <  1:1652066/150‑1 (MQ=255)
                                                                                                                          gtttgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGttt                            >  1:2135098/1‑150 (MQ=255)
                                                                                                                           tttgtttTCGATTTAAAAATATAATGAAATTATTTTTTTTTAAATATCGATATAACGATCAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGtttt                           <  1:4193440/150‑1 (MQ=255)
                                                                                                                              gtttTCTATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTAt                        <  1:1601189/150‑1 (MQ=255)
                                                                                                                                tttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGATAGGCAAAATAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGc                      >  1:3240004/1‑150 (MQ=255)
                                                                                                                                 ttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGcc                     <  1:2691032/150‑1 (MQ=255)
                                                                                                                                  tCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCg                    >  1:4619400/1‑150 (MQ=255)
                                                                                                                                  tCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCg                    >  2:2073671/1‑150 (MQ=255)
                                                                                                                                      tttCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAACATTATTCGCGGAGTGAAAGGCACAAAAACGGCCTCCCGAGAGGGAAGCCGTAGCAAAGTGCGCGGGTTTTTATGCCGGATg                >  2:3765195/1‑150 (MQ=255)
                                                                                                                                      tttCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATg                >  2:2259244/1‑150 (MQ=255)
                                                                                                                                       ttCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGc               >  2:677593/1‑150 (MQ=255)
                                                                                                                                       ttCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGc               >  2:3422539/1‑150 (MQ=255)
                                                                                                                                        tCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCg              >  2:3334737/1‑150 (MQ=255)
                                                                                                                                         cAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCgg             >  2:22253/1‑150 (MQ=255)
                                                                                                                                          aaaaTATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgt            >  1:4028389/1‑150 (MQ=255)
                                                                                                                                          aaaaTATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgt            >  1:4144866/1‑150 (MQ=255)
                                                                                                                                          aaaaTATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgt            <  1:1897246/150‑1 (MQ=255)
                                                                                                                                          aaaaTATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCAgt            >  1:2961132/1‑150 (MQ=255)
                                                                                                                                           aaaTATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgtg           >  1:4329872/1‑150 (MQ=255)
                                                                                                                                           aaaTATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgtg           >  1:3916870/1‑150 (MQ=255)
                                                                                                                                                 aaTGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACa     >  1:3638118/1‑150 (MQ=255)
                                                                                                                                                  aTGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACAt    <  2:4788538/150‑1 (MQ=255)
                                                                                                                                                    gAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATAc  >  1:3308210/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATAC  >  NC_000913/2177097‑2177394

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: