Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,304,798 T→A 8.0% intergenic (+405/+310) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,304,7980TA 8.0% 439.3 / 3.8 175intergenic (+405/+310)eco/mqoecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
Reads supporting (aligned to +/- strand):  ref base T (79/74);  new base A (13/2);  total (95/80)
Fisher's exact test for biased strand distribution p-value = 1.25e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 175 total aligned reads displayed.

GGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCG  >  NC_000913/2304650‑2304943
                                                                                                                                                    |                                                                                                                                                 
ttatatGGGGGTCACGCCGCATCCGACATCTAACGCCCTAGCGGGTTGCCTTATGCGACGCTGGCGCGTTTTATCTGGCCTACACCGCTGTTAAGTGCTCCACCCGTTAGGTTGTATTTGGCGGTTACGCCGCATCCGACATCCAACGTc                                                                                                                                                  <  1:1664415/148‑1 (MQ=11)
 gATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGAcc                                                                                                                                                 >  2:540411/1‑150 (MQ=21)
 gATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCATACACAGATGTGAAGTGCTCCACGACGTAGGTTGGATTTGGCGGTTACGCCGCATCCGACTTCGAACGGcc                                                                                                                                                 >  2:774074/1‑150 (MQ=11)
      ggCGGTTCCGCCGCATCCGACATCGACCGCCCCCGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGcc                                                                                                                                            <  2:2698291/150‑1 (MQ=11)
        cggcttcgccgcTTCCGACATCTCTGGCCCTAGCCGGTCGCCTTAGGGGACGGTGTCTCGTCTTTTCTGGCCTAAACCGCTGTGAAGTGCTCCACCCCGTTGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCgg                                                                                                                                          <  1:3379393/144‑1 (MQ=11)
        cGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCgg                                                                                                                                          >  1:3517682/1‑150 (MQ=37)
           ttACGCCTCATGCGCCAACTCGCGCCAATGCCGGTTGACTGTTGCTAAGCTGGGGGCTCTTCCGAGGACTACACCGCTGTGAAGTGCGGCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTg                                                                                                                                       <  1:2436000/150‑1 (MQ=2)
            caCGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGTATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGc                                                                                                                                      <  1:3661159/149‑1 (MQ=255)
              cgcggcATCCGACTTCTAACGCCCTAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCTGGGCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCt                                                                                                                                    <  2:2814036/150‑1 (MQ=11)
               gccgcATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTg                                                                                                                                   >  2:4283871/1‑150 (MQ=37)
               gccgcATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCAAACGACCGAGCCGGTTGCCTg                                                                                                                                   >  2:2175539/1‑150 (MQ=17)
                     tCCGACATCTAACGCCCTAGCGGGTTGCCTGATGCTACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGa                                                                                                                             <  1:755102/150‑1 (MQ=255)
                     tCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGa                                                                                                                             <  1:606146/150‑1 (MQ=37)
                      ccGACATCTACCGCCCAAGCCGTTTGCCTGTTGCGACGAGGGAGCGTCTTATCAGGCCTACACCGCTGTGATGTGCTCCACCCCGTAGGTTGGATATGGCGTTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGAc                                                                                                                            <  1:2338925/150‑1 (MQ=11)
                      ccGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGAc                                                                                                                            <  1:2338907/150‑1 (MQ=37)
                      ccGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGAc                                                                                                                            <  1:2338968/150‑1 (MQ=37)
                        gACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGc                                                                                                                          >  2:929085/1‑150 (MQ=37)
                        gACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGc                                                                                                                          >  1:3515478/1‑150 (MQ=37)
                        gACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGc                                                                                                                          >  2:4501227/1‑150 (MQ=37)
                              tAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCAAACGACCGAGCCGGTTGCCTGATGCGACGCTGgcgc                                                                                                                    >  2:1408869/1‑150 (MQ=17)
                                ccggcccaGCCTGTTGCCTGATGCGACGCTGGCGCGTCCTATCAGGCCTACACCGCTGTGAAGTGCTGCACCCCGTAGGTCTGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGt                                                                                                                  <  2:2199184/143‑1 (MQ=11)
                                aCGCCCCCGCCGGTTGCCTGATGCGACGGGGGCGCGTCTGATCAGGCCTACACGGCTGTCTAGTGATCGACCCCGTAGGTCCGACAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGt                                                                                                                  <  2:1307746/150‑1 (MQ=11)
                                  gCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGTGACGCTGGCGCGTCt                                                                                                                <  1:3409830/150‑1 (MQ=255)
                                     cgaGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTAt                                                                                                             <  1:4104216/148‑1 (MQ=37)
                                     cAAGCCGGTTTCCTGATGCGAGGCGGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTAt                                                                                                             <  2:47393/150‑1 (MQ=255)
                                      aaGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATc                                                                                                            >  2:3023567/1‑150 (MQ=37)
                                           ggTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGcc                                                                                                       <  1:1857003/150‑1 (MQ=39)
                                               gacttctGCGACGCTGTCGCGTCTTATCTGGCCTTCAACGAGGTGAATTGCTCCTGGCCGTAGGTGGGATAAGGCGGTTACGACGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTaca                                                                                                   <  2:3419250/144‑1 (MQ=11)
                                                 cTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAcc                                                                                                 >  2:3271765/1‑150 (MQ=39)
                                                  tGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCg                                                                                                <  2:438363/150‑1 (MQ=39)
                                                   gATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCATACACCGc                                                                                               >  2:3407044/1‑150 (MQ=255)
                                                     tGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCtg                                                                                             >  2:2555226/1‑150 (MQ=39)
                                                     tGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCtg                                                                                             <  1:4205443/150‑1 (MQ=39)
                                                     tGCGACGATGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCtg                                                                                             >  1:532420/1‑150 (MQ=255)
                                                        gACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCAAACACCGCTgaga                                                                                          >  1:1802672/1‑150 (MQ=255)
                                                         aCGCTGGCGCGTCTTATCAGGCCGACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGaa                                                                                         <  2:3052163/150‑1 (MQ=21)
                                                          cGCTGGCGCTTCTTATCAGGCCTACACCGCTGTGAAGTGGTCCTCCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGGCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAg                                                                                        <  2:51857/150‑1 (MQ=255)
                                                               gcgcGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTc                                                                                   <  2:3234972/150‑1 (MQ=39)
                                                                cgcgTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTcc                                                                                  >  2:3542960/1‑150 (MQ=21)
                                                                 gcgTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCa                                                                                 <  2:1210059/150‑1 (MQ=39)
                                                                 gcgTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCa                                                                                 <  2:3517682/150‑1 (MQ=39)
                                                                 gcgTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGACCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCACCACCCATCAGAAGTGCTccc                                                                                 >  2:1432516/1‑149 (MQ=11)
                                                                   gTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGgcg                                                                                                                     <  1:3690406/112‑1 (MQ=39)
                                                                     cTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCCCTGGGACGTGCGCcccccc                                                                             >  1:4532894/1‑150 (MQ=14)
                                                                       tATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGt                                                                           >  2:1783383/1‑150 (MQ=39)
                                                                       tATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGACCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACAGATGTGAAGTGCTCCACCCAGt                                                                           >  2:3022591/1‑150 (MQ=255)
                                                                             gCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGACCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACAGCTCAGACGTGCACCCCCCCGtctggcg                                                                     >  2:2347745/1‑144 (MQ=11)
                                                                                tACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGAt                                                                  >  1:4706038/1‑150 (MQ=255)
                                                                                   acCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAg                                                               >  2:2261184/1‑150 (MQ=255)
                                                                                    ccGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAgg                                                              <  1:684076/150‑1 (MQ=255)
                                                                                     cGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGAGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGc                                                             <  2:2557446/150‑1 (MQ=255)
                                                                                      gCTGTCCAGTGCTCCACCCCGTCGGTCCGATCAGGAGGTTCCTCCGCACCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCg                                                            <  2:3515500/150‑1 (MQ=11)
                                                                                       cTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCgg                                                           >  1:4004526/1‑150 (MQ=255)
                                                                                          tgAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTa                                                        >  2:696826/1‑150 (MQ=255)
                                                                                          cgAAGTGCGCCACCCCGCAGGTCTGATATTTCGGTAACGCCGCATCCGAGATCCAACGTCCGAGACGGTTTCCTGATGCGACGCTGGCTCTTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTa                                                        <  2:271010/149‑1 (MQ=11)
                                                                                           gAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCAGTAGGTCGGATCAGGCGGTTAc                                                       >  2:3726345/1‑150 (MQ=255)
                                                                                            aaGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTAcg                                                      <  1:3161292/150‑1 (MQ=255)
                                                                                            aaGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTAcg                                                      <  1:3795655/150‑1 (MQ=255)
                                                                                            aaGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTAcg                                                      <  2:2314797/150‑1 (MQ=255)
                                                                                              gTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTAcgcc                                                    >  1:979440/1‑150 (MQ=255)
                                                                                               tGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTAcgccg                                                   >  1:1036658/1‑150 (MQ=255)
                                                                                               tGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGGCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCCCTGTGGAGTGCTCCACCCCGTTGGTCGGATAAGGCGGTTAcgccg                                                   >  1:1036744/1‑150 (MQ=255)
                                                                                                   ccACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATc                                               <  2:3330151/150‑1 (MQ=37)
                                                                                                    cACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGAGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATcc                                              >  1:2903175/1‑150 (MQ=255)
                                                                                                    cACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGACCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCCCAACACCGCTGTGAAGTGCTCCACCCCGTAGGACGGATAACCCGGTTAAGCCGCATcc                                              >  1:453525/1‑150 (MQ=14)
                                                                                                      ccccGTAGGTTTGATAAGGCGGTTACGCCGAATCCGACATCCAACGTCCTAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGa                                            <  1:929006/150‑1 (MQ=255)
                                                                                                      ccccGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGa                                            <  1:929085/150‑1 (MQ=255)
                                                                                                         cGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCCTCCGACAt                                         >  2:805975/1‑150 (MQ=255)
                                                                                                         cGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGttg                                                                     <  1:1559845/122‑3 (MQ=18)
                                                                                                              gTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCAATACACACATGAGAATTGCAACACCCCGTATGTCGGATAAGTCGCAAACGCATAAGCCCACATCTAAc                                    >  2:1008479/1‑150 (MQ=9)
                                                                                                               ttGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACg                                   >  1:2237650/1‑150 (MQ=39)
                                                                                                               ttGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACg                                   >  1:3190622/1‑150 (MQ=39)
                                                                                                                tGGACAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGc                                  <  2:3740467/150‑1 (MQ=18)
                                                                                                                 ggATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGcc                                 >  1:3489837/1‑150 (MQ=38)
                                                                                                                 ggATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACCCGGGCGCCACAAATCAGGCCTACACCCCGCCGCAGTGCTCCACCCCGGAGGGCGGATAAGGCGGTTACGCCGCAGCCGACATcgcgcgcc                                 >  1:1214541/1‑143 (MQ=11)
                                                                                                                 ggATAAGGCGGTTACGCCGCATCCGACATCCAACGACCGAGCCGGTTGCCTGATGAGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCAAACCCGTAGGACGGATAAGGAGGTTAAGCCGCATCCGACATCTAACGcc                                 >  2:3730239/1‑150 (MQ=11)
                                                                                                                   aTAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGGGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGcccc                               >  1:45930/1‑149 (MQ=25)
                                                                                                                     aaGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAg                             >  1:2794547/1‑150 (MQ=34)
                                                                                                                       ggCGGTTCCGCCGCATCCGACATCCAACGTCCGCGCCGGTTGCGTGTTGCGTGGGTGGCGGTTCTTATCAGGCCTACACCGCTGTTATGTGCTCCACCCCGTTGGTCTGATATGGCGGTTACGCCGCATCCGAAATCTAACGCCCAAGcc                           <  1:1333547/150‑1 (MQ=11)
                                                                                                                       ggCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTTGGTCGGATATGGCGTTTACGCCGCATCCGACATCTAACGCCCAAGcc                           <  1:2555226/150‑1 (MQ=255)
                                                                                                                       ggCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGcc                           >  2:2963510/1‑150 (MQ=34)
                                                                                                                       ggCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCAAGcc                           >  1:430584/1‑150 (MQ=21)
                                                                                                                        gCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCg                          >  2:1120016/1‑150 (MQ=34)
                                                                                                                        gCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCAAGCCg                          >  2:4805309/1‑150 (MQ=21)
                                                                                                                        gCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGACTACACCCATGAGAAGTGCTCCACCCCGTAGGTCGGATCAGTCGGATACGCCGCAACCCACATCTAACTCCCAAGCCg                          >  2:648498/1‑150 (MQ=11)
                                                                                                                            gaccgccgcATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCtg                                                                                             >  2:2849481/4‑79 (MQ=21)
                                                                                                                                gccgcATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCt                   <  1:2953420/149‑1 (MQ=32)
                                                                                                                                gccgcATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTg                  <  2:4004526/150‑1 (MQ=32)
                                                                                                                                  cgcATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGAt                >  2:196393/1‑150 (MQ=32)
                                                                                                                                  cgcATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGAt                >  2:2060784/1‑150 (MQ=17)
                                                                                                                                     aTCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCg             >  1:1196854/1‑150 (MQ=32)
                                                                                                                                      tCCGACATCCAACGTCCGAGCCGGTTGCCTGATTCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCAAGCCGGTTGCCTGATGCGa            >  1:1330643/1‑150 (MQ=17)
                                                                                                                                      tCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCGAGCCGGTTGCCTGATGCGa            >  1:348908/1‑150 (MQ=17)
                                                                                                                                      tCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGa            >  2:3484888/1‑150 (MQ=32)
                                                                                                                                       ccGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGAc           <  2:1501843/150‑1 (MQ=32)
                                                                                                                                        cGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGACGTGCTCCACCCCGTAGGTCGGATAAGGCGGTAACGCCGCATCCCACATCTAACGCCCAAGCCGGTTGCCTGATGCGACg          >  2:4231357/1‑150 (MQ=12)
                                                                                                                                         gACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGc         >  2:1897609/1‑150 (MQ=32)
                                                                                                                                            aTCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATc                                                                                                            <  1:2781911/48‑1 (MQ=38)
                                                                                                                                              ccAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGgcg    >  2:1082394/1‑150 (MQ=32)
                                                                                                                                               cAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCAACACCGCTGTGAAATGCTCCACCCCGTATGTCGGATAAGGCGGTTACGCCGCAACCCACATCTAAAGCCCCAGCCGGTTGCCTGATGCGACGCTGgcgc   >  1:4118076/1‑150 (MQ=11)
                                                                                                                                                    |                                                                                                                                                 
GGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCG  >  NC_000913/2304650‑2304943

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: