| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 164,595 | T→G | 11.3% | intergenic (+61/‑135) | hrpB → / → mrcB | putative ATP‑dependent helicase/fused glycosyl transferase and transpeptidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 164,595 | 0 | T | G | 11.3% | 356.9 / 5.4 | 123 | intergenic (+61/‑135) | hrpB/mrcB | putative ATP‑dependent helicase/fused glycosyl transferase and transpeptidase |
| Reads supporting (aligned to +/- strand): ref base T (7/99); new base G (15/2); total (22/101) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.92e-12 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 132 total aligned reads displayed.
AGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGC‑AAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGA > NC_000913/164447‑164743 | agagATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGC‑AAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTtc > 2:2787680/1‑150 (MQ=255) cgatgatTGGGCGTTAACCCAAACATGTCGGGCGGGACGACCCGGA‑AAATACTGCACCGCCGCGAGGCACTAAAATGTTTTCTTATGGTTGG‑TTTTCTCCCGCTCCGTGGGGT‑TGTGGGCGGGGGTGTGGGCATCAGCGCGCACGTtcac < 2:1883129/149‑1 (MQ=255) ttttaatgggCTTTATCCCAAAAATGTCGGGCCTTACGACCGGCA‑AAATATGCCACCGACGCGAGGGACGAAAATTTATTCGTTTGTTTGT‑TTTTCTCCCTCTCCGTGTGGG‑TGTGGGCGGGGGTGTGGGCATCAGCGCGCACGTtcacc < 2:114620/143‑1 (MQ=255) tatcaaaGGGCTTTAACCCAAACATTACTGGCCGCACCACCCGCA‑AAAAACTCCACCCACGCGACGCACGAAAAATTATTCCTAAGTTTGT‑TTTTCTCCCACACCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcacc < 2:1994936/146‑1 (MQ=19) ttgggCGTTACCCAAAACTTGTGGGGCCGCACG‑CCCGGCAAAATACGCCACCGACGCTCCGCACCAAAATTTTTTCTTTTGGTTGT‑TTTTCCCCCTCTCCCTGTGGG‑TGCGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcaccctcac < 2:4460452/148‑1 (MQ=12) acgggCTTTAGCCCAAACATGTGTGGCCGGACGACCCGGA‑AATTAGTGCACCGACGCGACGGACGAAAATGTATTCTAACGGTTGT‑TTTTCTCCCTCCCCCTGTGGG‑TGTGGGCGGGGGTGCGGCCATCAGCGCGCACGTtcaccctcac < 2:295530/148‑1 (MQ=255) ggCGTTATCCCAAACATGTCTGGCCGGACGACCCGGC‑AAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtgacccccaaccc > 2:87242/1‑149 (MQ=255) cGTTATCCCAAACATGTCTGGCCGGACGACCCGGC‑AAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCCGCGCGCACGTGCACCCACACCCCaa > 2:646505/1‑150 (MQ=255) tATCCCAAACATGTCTGGCCGGACGACCCGGC‑AAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGGCCCGCACCCAAAacc > 1:771054/1‑150 (MQ=38) aTCCCAAACATGTCTGGCCGGACGACCCGGA‑AAATACTGCACGGACGCGACGGACGAAAATGTATTCGTATGGTTGG‑TTTTCTCCCTCTCCCTGTGGG‑TGAGGGCCGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCct < 2:3898376/150‑1 (MQ=38) tCCCAAACATGTCTGGCCGGACGACCCGGC‑AAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCctc < 2:3568958/150‑1 (MQ=255) cccAACCCTGTCTGGCCGGACGACCCGCA‑AAATAGTGCCCCGACGCGAGGGACTAAAATTTTTTGGTAGGTTTGT‑TTTTCTCCGTCTCCGGGTGGT‑CGTGGGCGGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCctct < 2:3705476/150‑1 (MQ=255) ccAAACATGTCTGGCCGGACGACCCGGC‑AAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGACCCCCACCCCAAaccacaa > 1:3346910/1‑146 (MQ=38) ccAAACATGTCTGGCCGGACGACCCGGC‑AAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGACCCCCACCCAAAcccacac > 1:2308812/1‑146 (MQ=38) tGTCTGGCCGGACGACCCGGC‑AAATACTGCACCGCCGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAg < 2:3176065/150‑1 (MQ=255) ggccggcccGGACGCCCCGCC‑AACTCCGGCACCGCCCCGACGGACCACCAAGTATTCGCACGGTGGG‑TTGGCGCCCGCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCCGCGCGCCCGTTCACCCTCACCCTAACCCTCTCCCTCAAg < 2:2739614/143‑1 (MQ=255) ggTCGGGCCGGACCCCCCGCC‑AAATACGGCACCGAGGCGCCGCACGAAAATTTATTGGTATGGTTGT‑TTTTCGCCCACCCCGGGGGGT‑TGTGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAg < 2:1729422/149‑1 (MQ=255) tCTGGCCGGACGACCCGGC‑AAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCAAACCCACACCCACCAggg > 2:4001971/1‑150 (MQ=38) tCTGGCCGGACGACCCGGC‑AAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGCCCCCCCCCCCGAAACCACTcccgcccggg > 1:277500/1‑143 (MQ=37) gccggccgCCCCGGA‑AAATCCTCCACCGACGCGACGGTCTAAAATTTTTTGTTATGTTTGG‑TTTTCTCCCTCGGCGTGTGGG‑TGCGGGCGGGGGTGAGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGagag < 1:2634351/150‑1 (MQ=255) gCCGGACGACCCGGC‑AAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCCCGTGCCCCCGCACCCCAACCCACACCCCCAAggggggg > 2:2954720/1‑146 (MQ=37) ccGGACGACCCGGC‑AAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTGGACCCACACCCAAAACCACACCCACAAcggcgggg > 2:4101536/1‑145 (MQ=25) ccGGACGACCCGGC‑AAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGGCCCCCACCCAAACCCACACCCACAACGGGGAgg > 2:4101359/1‑150 (MQ=37) cGGACGACCCGGC‑AAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGGGCACCCCCACCCAAAACCACACCCACCAGGGAGgggg > 1:1480559/1‑150 (MQ=37) cGGACGACCCGGC‑AAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAggg < 2:4723510/150‑1 (MQ=255) cGGACG‑CCCGGC‑AAAATCCGCCCCGCCGGGGCGGCCCTAAAAATTTTGCTTAGGTTGGTTTTTCACCCACCCCGTGGGGT‑CGGGGGCGGGGGTGCGGCCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTTGAGGGAGAggg < 2:1272845/150‑1 (MQ=255) ggACGACCCGGC‑AAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGCGCCACACCCAAAACCACAACCACAAGGGCGAgggg > 1:1426749/1‑150 (MQ=25) gacCCGTA‑AAAAACTGCCCCGCCGCGACGTACTAAATTTTATTCTTACGGTGGG‑TTTTCTCCCTCGCCGTGTGGG‑AGTGGGCCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTTGAGGGAGAgggttagg < 1:3500626/150‑6 (MQ=255) gacCCGGC‑AACTCCTCCACCGACGCGACGGCCGAAAAAGTATTCGTAAGGTTGG‑TTTACTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCCGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTTGAGGGAGAgggttagg < 2:2386457/150‑6 (MQ=37) cccGGC‑AAAAACGCCCCCGCGGCGACGGACTAAAACTCTTTCGTCAGGTTGG‑TTTTCCCCCGCCCCCGGGGGG‑CGTGGGCGGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGAt < 2:2183810/150‑1 (MQ=255) ccGCC‑AAATCCGGCCCCGCCGCGACGGACGAAAACGTTTTCGTAAGGTTGG‑TTTTCTCCCCCCCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATc < 2:2521740/150‑1 (MQ=37) gcaaaaTACTGCCCCGACGGGCGGGACTAAAATGTTTTCGTATGTTTGG‑TTTTCGCCGTGTCCCTGTGGG‑TGCGGGCGGGGGTGCGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGa < 2:4058975/147‑1 (MQ=255) gC‑AAATACTGCCCCGCCGCGCCGGACGAAAACTTATTCGTATGGTTGG‑TTTTCACCCTCTCCCTGTGGG‑TGAGGGCCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAg < 2:2512286/150‑1 (MQ=255) gC‑AAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGGGGGCCCCCACCCGAAACCACGCCCTCAAGGGGGAGGGGGCCGGTCGAg > 1:746039/1‑150 (MQ=25) gC‑AAATACGGCACCGCCGCGCCGGACGAAACAGTATTCGTATGGTTGG‑TTTTCGCCCTCTCCCTGTGGG‑AGTGGGCGGGGGTGAGGGCATCAGCGCGCACGGTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAg < 2:3895376/150‑1 (MQ=38) cc‑AAAGACTGCCCCGACGGGCCGGACCAAAAAGTATTCGTACGGTGGG‑TTTTCGCCCTCTCCCTGGGGG‑AGTGGGCCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAg < 1:2755647/149‑1 (MQ=255) c‑AAATCCTCCACCGAGGCGCCGGACCAAAAAGTATTCGAACGTTTGT‑TTTACCCCCACTCACTGGGGG‑AGCGGGCGGGGGTTTGGGCATCAGCGCGCCCGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGc < 1:342522/150‑1 (MQ=255) aaaTCCGGCACCGCGGCGCCGGTTGAAAATGTTTTGGTCTGGTTGG‑TTTTCGCCCTCGCCGGGGGGG‑AGTGGGCGGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGca < 1:3458360/150‑1 (MQ=255) cACCGCCGCTACGCACAAAAAATTATTCTTATGGTTTT‑TTTTCCCCCTCTCCGTGTGTT‑AGCGGGCGGGGGTGAGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAAtttt < 1:3262724/150‑1 (MQ=255) ccgccgCGACGGCCCAAAATTTTTTCTTATGGTTGG‑TTTTCCCCCCCCCCGTGGGGG‑TGTGGGGGGGGGTGCGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTga < 1:1469459/150‑1 (MQ=255) ccgccgCGACGGACGAAAACGTATTCGTAAGGTTGG‑TTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTga < 2:3588470/150‑1 (MQ=255) ccGACGCGCCGGACCAAAATTTATTCGTACGTTTGG‑TTTTCTCCCACTCCCTGTGGT‑TGCGGGCGGGGTTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTga < 2:3834234/150‑1 (MQ=255) cgacgcgacgTCCTATATATTATTCTTAGGCTTGG‑TTTTCGCCCGCGCCCTTTGGT‑TGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTgag < 2:1473763/150‑1 (MQ=255) gacgcgacgGACTAAAATGTATTCGTATGGTTGG‑TTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTgaga < 2:777288/150‑1 (MQ=255) acgcgacgGACGAAAAAGTATTCGTAAGGTTGG‑TTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGCACCCACACCCCAAACCCCACCCACACCGGGGAGGGGGCCGCGACAGCACCAAATTTgagtg > 2:3052020/1‑148 (MQ=21) gtaggAAAAAGTATTCGTAAGGTTGG‑TTTTCTCCCTCTCCCGGTGGG‑AGTGGGCGGGGGTTAGGGCCTCAGCGCGCACGTTCACCCTCACGCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGTGATAtctt < 2:2737265/146‑1 (MQ=255) taaaaaGTATTCGTAAGGTTGG‑TTTTCTCCCTCTCCCTGTGGG‑AGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtcttcttc < 2:1153873/149‑1 (MQ=255) gAAAAAGTATTCGTAAGGTTGG‑TTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCCCAAGGGAGAGGGGACCGTTCGAGCACAAATTTTGAGAGATAtcttcttc < 2:3912934/150‑1 (MQ=255) gAAAAAGTATTCGTAAGGTTGG‑TTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCACACACGAAAACACTAACGCCAGGGAGAGGGGGCCCATCGAGCACAAAATTTGTGAGAAAtattctta > 2:747288/1‑149 (MQ=21) aaaaaGTATTCGTACGGTTGG‑TTTACTCCCTCACCATGTGGG‑AGAGGGCCGGGGTGAGGGCATCCGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtcttcttct < 2:1075304/150‑1 (MQ=255) aaaGTATTCGTACGGTTGG‑TTTTCTCCCTCTCCCGGTGGG‑AGAGGGCCGGGGTGAGGGACTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGt < 2:2731739/150‑1 (MQ=255) aaGTATTCGTAAGGTTGG‑TTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCAACCGCACCCAAAACCACTACCACAACGGGGAGGGGGCCCATCGAGCACAAAATTTGTGAGATATATTATTCTGtg > 2:4284166/1‑149 (MQ=21) ttATTCGTAAGGTTGG‑TCTGCCCCCCCCCCCGGTGGG‑TGTGGGCGGGGGTGCGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCtt < 1:428416/149‑1 (MQ=255) gTATTCGTATGGTTGT‑TTTTCTCCATCTCCCTGTGGG‑ATTGGGCCGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACTAATTTTGAGAGATATCTTCTTCTGTCtt < 2:380627/150‑1 (MQ=255) ttttCGTTTGGTTGG‑TTTTCTCCCTCTCCCTGTGGG‑TGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTg < 2:2797492/148‑1 (MQ=255) ttttCGTATGTTTGG‑TTTTCTCCCTCTCCCGGGGGG‑AGTGGGCGGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTg < 1:923438/148‑1 (MQ=255) tttgcGTAAGGTTTG‑TTTTCTCCCTCTCCCTTTGGG‑TGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTg < 2:2292383/146‑1 (MQ=39) tATTCGTTTGGTTGT‑TTTTCTCCCGCTCCGTGGGGG‑AGTGGGCGGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTg < 1:923419/150‑1 (MQ=255) tggTATGGTTGG‑TTTCCGCCCCCGCCCTGGGGG‑TGCGGGCGGGGGTGGGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTaa < 2:1816158/148‑1 (MQ=255) tCGTATGGTTGG‑TTTTCTCCCTCGCCCGGTGGG‑TGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTaa < 2:4296628/150‑1 (MQ=39) tCGTATGGTTGG‑TTTTCCCCCTCTCCCTGTGGT‑TGCGGGCGGGGGTGCGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTaa < 2:1816316/150‑1 (MQ=255) gTAAGGTTGG‑TTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACa < 2:3158360/150‑1 (MQ=255) taGGTTGT‑TTTTCTCCCTCCCCGTGTGGG‑TGAGGGCCGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACaga < 1:2637701/149‑1 (MQ=255) atgtttgg‑ttTTCGCCCTCCCCCTGTGGT‑TGTGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACaga < 1:2637504/146‑1 (MQ=255) tgg‑ttTTCTCCCTCTCCCTGTGGG‑AGCGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAAc < 1:756927/150‑1 (MQ=255) tgg‑ttTACTCCCTCTCCCTGGGGG‑CGTGGGCCGGGTTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAAc < 1:2297629/150‑1 (MQ=255) g‑ttTTCCCCCCCCCCCGGGGGGTAGTGGGCGGGGGTGCGGCCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGACGAACa < 1:1281904/150‑1 (MQ=255) ttttCTCCCTCTCCCTGTGGG‑AGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGa < 1:1174195/150‑1 (MQ=255) ttttCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGa < 1:1174443/150‑1 (MQ=255) tccctctccctGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATc < 2:3125609/150‑1 (MQ=255) tccctctcccgGTGGG‑AGAGGGCGGGGGTGAGGGCATCCGCGCGCACGTTCACCCTCACCCTAACCCTCTCACTCAAGGGAGAGGGGACCGATCGAGCCCAAATTTTGAGAGATATCTTCTTCTGTATTGTAACAGAAGAACAGAATATc < 2:2394215/150‑1 (MQ=255) gccctctccctGTGGG‑AGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGGTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATc < 2:3125751/149‑1 (MQ=255) tgGG‑TGTGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTgcgc < 2:4174898/150‑1 (MQ=255) gggg‑AGTGGGCGGGGGTGCGGGCCTCCGCGCGCACGTTCACCCTCACCCTTCCCCTCTCCCTCAAGGGAGAGGGGACCGATCGCGCACAAATTTTGAGAGATATCTTCTTCTGCCTTGTAACAGAAGAACAGAAAATCGGGCTTTTgcgc < 2:1879098/149‑1 (MQ=255) ccGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTg < 2:3140408/150‑1 (MQ=255) ggggggAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCg < 2:812043/150‑1 (MQ=255) ggggTGCGGGCCTCCGCGCGCCCGGTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCg < 1:4548320/150‑1 (MQ=255) ggggTGCGGGCCTCCGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCg < 1:4548262/150‑1 (MQ=255) ggggTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTACCCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCg < 1:4296447/150‑1 (MQ=255) ggggTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTACCCCTCTCCCTCAAGGGAGAGGGGACCGAGCGAGCCCAAATTTTGAGAGATATCTTCTTCGGTCTTGTAACAGAAGAACAGAAACTCGGGCTTTTGCGCCTGAATATTGCg < 2:2246729/150‑1 (MQ=255) ggggTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCg < 2:2928212/150‑1 (MQ=255) ggggTGCGGCCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCg < 1:1799984/150‑1 (MQ=255) ggggTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCg < 1:4599114/150‑1 (MQ=255) ggggTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCg < 1:2787680/150‑1 (MQ=255) ggggTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCg < 1:1521026/150‑1 (MQ=255) ggggTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCg < 2:3571517/150‑1 (MQ=255) ggggTGAGGCCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTACTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCg < 1:3052020/150‑1 (MQ=255) gAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGaa < 1:4075084/150‑1 (MQ=255) ggCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGaaaaa < 2:679432/150‑1 (MQ=255) cATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGc < 2:4219898/150‑1 (MQ=255) cATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGc < 2:3701742/150‑1 (MQ=255) aGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCTAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTATTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATgg < 2:4529217/150‑1 (MQ=255) cgcACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCgg < 2:3945529/150‑1 (MQ=255) cgcACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCgg < 2:277500/150‑1 (MQ=255) cgcACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCgg < 2:1226662/150‑1 (MQ=255) cGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAAt < 2:745934/150‑1 (MQ=255) cGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAAt < 2:746039/150‑1 (MQ=255) cGTTCACCCTCACCCTAACCCTCACCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAAt > 2:1106870/1‑150 (MQ=255) gTTCACCCACACCCAAACCCACACCCTCAAGGGAGAGGGGACCGATCGAGCACAAAATTTGAGAGATATATTCATATGTCTTGTAAAAGAAGAACAGAAAAACGGGCTTTTGCGCCGGAATATTTCGGAGAAAAAGCATGGCCGGGAATg > 2:598412/1‑150 (MQ=255) gtcaccctcacccTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGa < 1:4001971/149‑1 (MQ=255) | AGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGC‑AAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGA > NC_000913/164447‑164743 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |