Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 173,573 T→G 5.2% intergenic (+129/+29) fhuB → / ← hemL iron(3+)‑hydroxamate import ABC transporter permease/glutamate‑1‑semialdehyde aminotransferase (aminomutase)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913173,5730TG 5.2% 1278.1 / 47.5 349intergenic (+129/+29)fhuB/hemLiron(3+)‑hydroxamate import ABC transporter permease/glutamate‑1‑semialdehyde aminotransferase (aminomutase)
Reads supporting (aligned to +/- strand):  ref base T (165/165);  new base G (8/10);  total (174/175)
Fisher's exact test for biased strand distribution p-value = 8.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.35e-01

Only 100 of 350 total aligned reads displayed.

TTGAGAAAGCAGAGCCGTTAATTTTTCCATATAACGGCCCTGTACGCCTGGACGGATAAAGCGTTTACGCGACAACGGATGCCCGATGCGACGCTGGCGCGTCTTATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTCCATGCTGTGCGCCACGGACATAAAGCCCGCTTCAAACGCTGACGGTGCCAGGTAAACACCTTCGT  >  NC_000913/173424‑173720
                                                                                                                                                     |                                                                                                                                                   
     acagcagcGCCGTTAATTTGTCCATATAACGGCCCTGTACGCCTGGACGGATACAGCGTTTACGCGACAACGGAGGCCCGATGCGACGCTGGCGCGTCTTATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCAcgc                                                                                                                                                <  2:83799/148‑1 (MQ=255)
     aaaGCAGAGCCGTTAATTTTTCCATATAACGGCCCTGTACGCCTGGACGGATAAAGCGTTTACGCGACAACGGATGCCCGATGCGACGCTGGCGCGTCTTATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCAcgc                                                                                                                                                <  2:1145230/150‑1 (MQ=255)
      aaGCAGAGCCGTTAATTTTTCCATATAACGGCCCTGTACGCCTGGACGGATAAAGCGTTTACGCGACAACGGATGCCCGATGCGACGCTGGCGCGTCTTATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCAcgcc                                                                                                                                               <  1:281192/150‑1 (MQ=255)
          agagCCGTTAATTTTTCCATATACCGGCCCTGTACGCCTGGACGGATAAAGCGTTTACGCGACAACGGATGCCCGATGCGACGCTGGCGCGTCTTATCTGGCCTACACACGGCCCCGAATCGTAGGTCGGATGCGGCGTGAAcgccttat                                                                                                                                           <  1:4553386/150‑5 (MQ=38)
          agagCCGTTAATTTTTCCATAAAACCGCCCAGTACGCCGGGACGGATAAAGCGTTTACGCCACAACCGATGCCCGATGCGACGCTGGCGCGTCTTATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGCTAAGGCGTTCACGCCGCAt                                                                                                                                           >  2:3442676/1‑150 (MQ=38)
              ccGTTAATTTTTCCATATAACGGCCCTGTAAGCCTGGACGGATAAAGCGTTTACGCGACAACGGATGCCCGATGCGACGCTGGCGCGTCTTATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGa                                                                                                                                       >  1:3741501/1‑150 (MQ=255)
              ccGTTAATTTTACCATATAACGGCCCTGTACGCCTGGACGGATAAAGCGTTTACGCGACAACGGATGCCCGATGCGACTCTGGCGCGTCTTATGGGGCCTACAAAGGGCGCCGAATCGTAGGTCGGATGCGGCGTGAACGCCTTATCCGa                                                                                                                                       <  1:3630387/150‑1 (MQ=255)
               cGTTAATTTTTCCATATAACGGCCCTGTACGCCTGGACGGATAAAGCGTTTACGCGACAACGGATGCCCGATGCGACGCTGGCGCGTCTTATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATGCGGCGTGAACGCCTTATCCGAc                                                                                                                                      <  1:1065406/150‑1 (MQ=39)
                gTTAATTTTTCCATATAACGGCCCTGTACGCCTGGACGGATAAAGCGTTTACGCGACAACGGATGCCCGATGCGACGCTGGCGCGTCTTATCGGGCCTATAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACa                                                                                                                                     >  1:1950154/1‑150 (MQ=255)
                gTTAATTTTTCCATATAACGGCCCTGTACGCCTGGACGGATAAAGCGTTTACGCGACAACGGATGCCCGATGCGACGCTGGCGCGTCTTATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACa                                                                                                                                     >  2:3051017/1‑150 (MQ=255)
                 ttAATTTTTCCATATAACGGCCCTGTACGCCTGGACGGATAAAGCGTTTACGCGACAACGGATGCCCGATGCGACGCTGGCGCGTCTTATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACaa                                                                                                                                    >  2:3776363/1‑150 (MQ=255)
                     tttttCCATCTAACGGCCCTGTACGCCTGGACGGATAAAGCGTTTACGCGACAACGGATGCCCGATGCGACGCTGGCGCGTCTTATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCa                                                                                                                                <  2:2910826/150‑1 (MQ=255)
                     tttttCCATATAACGGCCCTGTACGCCTGGACGGATAAAGCGTTTACGCGACAACGGATGCCCGATGCGACGCTGGCGCGTCTTATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCa                                                                                                                                <  2:2385360/150‑1 (MQ=255)
                        ttCCATATAACGGCCCTGTACGCCTGGACGGATAAAGCGTTTACGCGACAACGGATGCCCGATGCGACGCTGGCGCGTCTTATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGc                                                                                                                             >  1:2393421/1‑150 (MQ=255)
                        ntCCATATAACGGCCCTGTACGCCTGGACGGATAAAGCGTTTACGCGACAACGGATGCCCGATGCGACGCTGGCGCGTCTTATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGc                                                                                                                             >  1:2538579/2‑150 (MQ=255)
                         tCCATATAACGGCCCTGTACGCCTGGACGGATAAAGCGTTTACGCGACAACGGATGCCCGATGCGACGCTGGCGCGTCTTATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCt                                                                                                                            <  2:4589070/150‑1 (MQ=255)
                         tCCATATAACGGCCCTGTACGCCTGGACGGATAAAGCGTTTACGCGACAACGGATGCCCGATGCGACGCTGGCGCGTCTTATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCt                                                                                                                            >  2:2431752/1‑150 (MQ=255)
                         tCCATATAACGGCCCTGTACGCCTGGACGGATAAAGCGTTTACGCGACAACGGATGCCCGATGCGACGCTGGCGCGTCTTATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCt                                                                                                                            <  2:1835952/150‑1 (MQ=255)
                         tCCATATAACGGCCCTGTACGCCTGGACGGATAAAGCGTTTACGCGACAACGGATGCCCGATGCGACGCTGGCGCGTCTTATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCt                                                                                                                            >  2:3884279/1‑150 (MQ=255)
                              ataACGGCCCTGTACGCCTGGACGGATAAAGCGTTTACGCGACAACGGATGCCCGATGCGACGCTGGCGCGTCTTATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATc                                                                                                                       >  1:417193/1‑150 (MQ=255)
                               taACGGCCCTGTACGCCTGGACGGATAAAGCGTTTACGCGACAACGGATGCCCGATGCGACGCTGGCGCGTCTTATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATca                                                                                                                      <  1:131936/150‑1 (MQ=255)
                               taACGGCCCTGTACGCCTGGACGGATAAAGCGTTTACGCGACAACGGATGCCCGATGCGACGCTGGCGCGTCTTATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATca                                                                                                                      <  1:1948511/150‑1 (MQ=255)
                                  cGGCCCTGTACGCCTGGACGGATAAAGCGTTTACGCGACAACGGATGCCCGATGCGACGCTGGCGCGTCTTATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACaa                                                                                                                   >  2:1623315/1‑150 (MQ=255)
                                  cGGCCCTGTACGCCTGGACGGATAAAGCGTTTACGCGACAACGGATGCCCGATGCGACGCTGGCGCGTCTTATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACaa                                                                                                                   <  1:3970698/150‑1 (MQ=255)
                                  cGGCCCTGTACGCCTGGACGGATAAAGCGTTTACGCGACAACGGATGCCCGATGCGACGCTGGCGCGTCTTATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACaa                                                                                                                   >  1:1015822/1‑150 (MQ=255)
                                     cccTGTACGCCTGGACGGATAAAGCGTTTACGCGACAACGGATGCCCGATGCGACGCTGGCGCGTCTTATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGAt                                                                                                                        >  1:337564/1‑142 (MQ=255)
                                      ccTGTACGCCTGGACGGATAAAGCGTTTACGCGACAACGGATGCCCGATGCGACGCTGGCGCGTCTTATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTc                                                                                                               <  2:725348/150‑1 (MQ=255)
                                       cTGTACGCCTGGACGGATAAAGCGTTTACGCGACAACGGATGCCCGATGCGACGCTGGCGCGTCTTATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCg                                                                                                              >  1:990340/1‑150 (MQ=255)
                                         gTACGCCTGGACGGATAAAGCGTTTACGCGACAACGGATGCCCGATGCGACGCTGGCGCGTCTTATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCa                                                                                                            <  1:2636261/150‑1 (MQ=255)
                                         gTACGCCTGGACGGATAAAGCGTTTACGCGACAACGGATGCCCGATGCGACGCTGGCGCGTCTTATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCa                                                                                                            <  1:4580422/150‑1 (MQ=255)
                                             gCCTGGACGGATAAAGCGTTTACGCGACAACGGATGCCCGATGCGACGCTGGCGCGTCTTATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAaca                                                                                                        <  1:3826304/150‑1 (MQ=255)
                                               cTGGACGGATAAAGCGTTTACGCGACAACGGATGCCCGATGCGACGCTGGCGCGTCTTATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACAcc                                                                                                      <  1:4435228/150‑1 (MQ=255)
                                                tGGACGGATAAAGCGTTTACGCGACAACGGATGCCCGATGCGACGCTGGCGCGTCTTATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACAccc                                                                                                     <  2:3075578/150‑1 (MQ=255)
                                                  gACGGATAAAGCGTTTACGCGACAACGGATGCCCGATGCGACGCTGGCGCGTCTTATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGa                                                                                                   >  2:1977062/1‑150 (MQ=255)
                                                     ggATAAAGCGTTTACGCGACAACGGATGCCCGATGCGACGCTGGCGCGTCTTATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGt                                                                                                >  2:3735744/1‑150 (MQ=255)
                                                       aTAAAGCGTTTACGCGACAACGGATGCCCGATGCGACGCTGGCGCGTCTTATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTgc                                                                                              <  1:4042492/150‑1 (MQ=255)
                                                       aTAAAGCGTTTACGCGACAACGGATGCCCGATGCGACGCTGGCGCGTCTTATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTgc                                                                                              >  2:492961/1‑150 (MQ=255)
                                                        tAAAGCGTTTACGCGACAACGGATGCCCGATGCGACGCTGGCGCGTCTTATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTgca                                                                                             <  1:179293/150‑1 (MQ=255)
                                                         aaaGCGTTTACGCGACAACGGATGCCCGATGCGACGCTGGCGCGTCTTATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTgcag                                                                                            <  1:104033/150‑1 (MQ=255)
                                                           aGCGTTTACGCGACAACGGATGCCCGATGCGACGCTGGCGCGTCTTATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTgcagca                                                                                          >  2:1370796/1‑150 (MQ=255)
                                                             cGTTTACGCGACAACGGATGCCCGATGCGACGCTGGCGCGTCTTATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATc                                                                                        <  2:4686697/150‑1 (MQ=255)
                                                                ttACGCGACAACGGATGCCCGATGCGACGCTGGCGCGTCTTATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGAt                                                                                     <  1:3645440/150‑1 (MQ=255)
                                                                ttACGCGACAACGGATGCCCGATGCGACGCTGGCGCGTCTTATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGAt                                                                                     <  2:2547258/150‑1 (MQ=255)
                                                                ttACGCGACAACGGATGCCCGATGCGACGCTGGCGCGTCTTATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGAt                                                                                     <  1:4335172/150‑1 (MQ=255)
                                                                   cgcgACAACGGATGCCCGATGCGACGCTGGCGCGTCTTATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGgt                                                                                  <  1:1487812/150‑1 (MQ=255)
                                                                    gcgACAACGGATGCCCGATGCGACGCTGGCGCGTCTTATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGgtg                                                                                 >  2:4045325/1‑150 (MQ=255)
                                                                        cAACGGATGCCCGATGCGACGCTGGCGCGTCTTATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTAt                                                                             <  2:3930833/150‑1 (MQ=255)
                                                                         aaCGGATGCCCGATGCGACGCTGGCGCGTCTTATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTAtt                                                                            <  2:41089/150‑1 (MQ=255)
                                                                         aaCGGATGCCCGATGCGACGCTGGCGCGTCTTATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTAtt                                                                            <  2:2178493/150‑1 (MQ=255)
                                                                           cGGATGCCCGATGCGACGCTGGCGCGTCTTATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGa                                                                          <  2:3622616/150‑1 (MQ=255)
                                                                           cGGATGCCCGATGCGACGCTGGCGCGTCTTATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGa                                                                          <  2:3496598/150‑1 (MQ=255)
                                                                            ggATGCCCGATGCGACGCTGGCGCGTCTTATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATAATGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGat                                                                         >  1:4126994/1‑150 (MQ=255)
                                                                                  ccGATGCGACGCTGGCGCGTCTTATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTc                                                                   >  1:310673/1‑150 (MQ=255)
                                                                                  ccGATGCGACGCTGGCGCGTCTTATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTc                                                                   >  2:2229369/1‑150 (MQ=255)
                                                                                  ccGATGCGACGCTGGCGCGTCTTATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCACATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTc                                                                   <  2:1866967/150‑1 (MQ=255)
                                                                                   cGATGCGACGCTGGCGCGTCTTATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTcc                                                                  <  1:465364/150‑1 (MQ=255)
                                                                                    gATGCGACGCTGGCGCGTCTTATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTCCa                                                                 >  1:4781960/1‑150 (MQ=255)
                                                                                         gACGCTGGCGCGTCTTATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTCCATGCtg                                                            >  1:593793/1‑150 (MQ=255)
                                                                                         gACGCTGGCGCGTCTTATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTCCATGCtg                                                            >  2:398838/1‑150 (MQ=255)
                                                                                         gACGCTGGCGCGTCTTATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTAGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTCCATGCtg                                                            >  1:431356/1‑150 (MQ=255)
                                                                                          aCGCTGGCGCGTCTTATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTCCATGCtgt                                                           <  1:2875155/150‑1 (MQ=255)
                                                                                            gCTGGCGCGTCTTATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTCCATGCTGTgc                                                         <  2:4488183/150‑1 (MQ=255)
                                                                                             cTGGCGCGTCTTATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTCCATGCTGTgcg                                                        >  2:3186994/1‑150 (MQ=255)
                                                                                              tGGCGCGTCTTATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTCCATGCTGTgcgc                                                       >  2:539529/1‑150 (MQ=255)
                                                                                                 cgcgTCTTATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTCCATGCTGTGCGCCAc                                                    <  1:3776363/150‑1 (MQ=255)
                                                                                                  gcgTCTTATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTCCATGCTGTGCGCCACg                                                   <  1:183517/150‑1 (MQ=255)
                                                                                                  gcgTCTTATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTCCATGCTGTGCGCCACg                                                   >  1:3086132/1‑150 (MQ=255)
                                                                                                    gTCTTATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTCCATGCTGTGCGCCACGGa                                                 <  1:126505/150‑1 (MQ=255)
                                                                                                     tCTTATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTCCATGCTGTGCGCCACGGAc                                                >  2:3361995/1‑150 (MQ=255)
                                                                                                     tCTTATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTCCATGCTGTGCGCCACGGAc                                                >  2:1237981/1‑150 (MQ=255)
                                                                                                          tCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTCCATGCTGTGCGCCACGGACATaaa                                           <  1:1576650/150‑1 (MQ=255)
                                                                                                          tCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTCCATGCTGTGCGCCACGGACATaaa                                           <  1:3481665/150‑1 (MQ=255)
                                                                                                            gggCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTCCATGCTGTGCGCCACGGACATAAAGc                                         >  1:2189438/1‑150 (MQ=255)
                                                                                                              gCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTCCATGCTGTGCGCCACGGACATAAAGccc                                       >  2:363752/1‑150 (MQ=255)
                                                                                                               ccTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTCCATGCTGTGCGCCACGGACATAAAGCCCg                                      >  1:2585384/1‑150 (MQ=255)
                                                                                                               ccTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTCCATGCTGTGCGCCACGGACATAAAGCCCg                                      >  2:1503120/1‑150 (MQ=255)
                                                                                                                  aCAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTCCATGCTGTGCGCCACGGACATAAAGCCCGCtt                                   <  2:1586656/150‑1 (MQ=255)
                                                                                                                      aCGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTCCATGCTGTGCGCCACGGACATAAAGCCCGCTTCaaa                               <  2:2796017/150‑1 (MQ=255)
                                                                                                                        ggCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTCCATGCTGTGCGCCACGGACATAAAGCCCGCTTCAAACg                             >  2:321786/1‑150 (MQ=255)
                                                                                                                         gCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTCCATGCTGTGCGCCACGGACATAAAGCCCGCTTCAAACGc                            <  1:1108550/150‑1 (MQ=255)
                                                                                                                            ccGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTCCATGCTGTGCGCCACGGACATAAAGCCCGCTTCAAACGCTGa                         >  1:2338494/1‑150 (MQ=255)
                                                                                                                             cGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTCCATGCTGTGCGCCACGGACATAAAGCCCGCTTCAAACGCTGAc                        >  2:2712532/1‑150 (MQ=255)
                                                                                                                             cGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTCCATGCTGTGCGCCACGGACATAAAGCCCGCTTCAAACGCTGAc                        >  2:2775333/1‑150 (MQ=255)
                                                                                                                                aTCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTCCATGCTGTGTGCCACGGACATAAAGCCCGCTTCAAACGCTGACGGt                     <  2:2855501/150‑1 (MQ=255)
                                                                                                                                    tAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTCCATGCTGTGCGCCACGGACATAAAGCCCGCTTCAAACGCTGACGGTGCCa                 >  2:2158797/1‑150 (MQ=255)
                                                                                                                                    tAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTCCATGCTGTGCGCCACGGACATAAAGCCCGCTTCAAACGCTGACGGTGCCa                 >  1:371381/1‑150 (MQ=255)
                                                                                                                                     aGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTCCATGCTGTGCGCCACGGACATAAAGCCCGCTTCAAACGCTGACGGTGCCAg                >  1:4639775/1‑150 (MQ=255)
                                                                                                                                       gTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTCCATGCTGTGCGCCACGGACATAAAGCCCGCTTCAAACGCTGACGGTGCCAGGt              >  2:581143/1‑150 (MQ=255)
                                                                                                                                        tCGGATGCGGCGTGAACGCCTTATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTCCATGCTGTGCGCCACGGACATAAAGCCCGCTTCAAACGCTGACGGTGCCAGGTa             >  1:2033945/1‑150 (MQ=255)
                                                                                                                                        tCGGATGCGGCGTGAACGCCTTATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTCCATGCTGTGCGCCACGGACATAAAGCCCGCTTCAAACGCTGACGGTGCCAGGTa             >  1:90584/1‑150 (MQ=255)
                                                                                                                                            aTAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTCCATGCTGTGCGCCACGGACATAAAGCCCGCTTCAAACGCTGACGGTGCCAGGTAAaca         <  2:2291971/150‑1 (MQ=255)
                                                                                                                                            aTAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTCCATGCTGTGCGCCACGGACATAAAGCCCGCTTCAAACGCTGACGGTGCCAGGTAAaca         <  2:3580229/150‑1 (MQ=255)
                                                                                                                                             tAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTCCATGCTGTGCGCCACGGACATAAAGCCCGCTTCAAACGCTGACGGTGCCAGGTAAacac        <  1:562114/150‑1 (MQ=255)
                                                                                                                                              aaGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTCCATGCTGTGCGCCACGGACATAAAGCCCGCTTCAAACGCTGACGGTGCCAGGTAAACAcc       >  2:4396238/1‑150 (MQ=255)
                                                                                                                                               aGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTCCATGCTGTGCGCCACGGACATAAAGCCCGCTTCAAACGCTGACGGTGCCAGGTAAACACCt      >  1:2745230/1‑150 (MQ=255)
                                                                                                                                               aGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTCCATGCTGTGCGCCACGGACATAAAGCCCGCTTCAAACGCTGACGGTGCCAGGTAAACACCt      >  2:3156521/1‑150 (MQ=255)
                                                                                                                                                 gCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTCCATGCTGTGCGCCACGGACATAAAGCCCGCTTCAAACGCTGACGGTGCCAGGTAAACACCTTc    <  2:191652/150‑1 (MQ=255)
                                                                                                                                                 gCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTCCATGCTGTGCGCCACGGACATAAAGCCCGCTTCAAACGCTGACGGTGCCAGGTAAACACCTTc    <  2:1063481/150‑1 (MQ=255)
                                                                                                                                                  cGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTCCATGCTGTGCGCCACGGACATAAAGCCCGCTTCAAACGCTGACGGTGCCAGGTAAACACCTTCg   >  2:4153226/1‑150 (MQ=255)
                                                                                                                                                   gTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCGTCCATGCTGTGCGCCACGGACATAAAGCCCGCTTCAAACGCTGACGGTGCCAGGTAAACACCTTCGt  <  1:835186/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
TTGAGAAAGCAGAGCCGTTAATTTTTCCATATAACGGCCCTGTACGCCTGGACGGATAAAGCGTTTACGCGACAACGGATGCCCGATGCGACGCTGGCGCGTCTTATCGGGCCTACAAACGGCCCCGAATCGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACAAACCATGCTGGATCACAACTTCGCAAACACCCGACGTGCAGCATCGATGGTGTTATTGATATCTTCCATGCTGTGCGCCACGGACATAAAGCCCGCTTCAAACGCTGACGGTGCCAGGTAAACACCTTCGT  >  NC_000913/173424‑173720

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: