Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,987,104 T→A 5.3% intergenic (+28/‑432) yqeG → / → yqeH putative transporter/putative LuxR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,987,1040TA 5.3% 957.9 / 30.8 264intergenic (+28/‑432)yqeG/yqeHputative transporter/putative LuxR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (115/135);  new base A (4/10);  total (119/145)
Fisher's exact test for biased strand distribution p-value = 2.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01

Only 100 of 279 total aligned reads displayed.

TTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATAAGACTATTTAAGATGCATAACTTA  >  NC_000913/2986955‑2987253
                                                                                                                                                     |                                                                                                                                                     
ttttCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCt                                                                                                                                                       <  2:1931354/150‑1 (MQ=255)
  ttCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTAc                                                                                                                                                     >  2:4585591/1‑150 (MQ=255)
     atcatGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAAt                                                                                                                                                  <  1:2982543/150‑1 (MQ=255)
      tcatGCCTACGCCGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAAtt                                                                                                                                                 >  2:2579157/1‑150 (MQ=255)
          gCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGAATGGTTCAAAATTGTAGCAATTTTGa                                                                                                                                             <  2:1889221/150‑1 (MQ=255)
          gCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGAATGGTTCAAAATTGTAGCAATTTTGa                                                                                                                                             <  2:1280453/150‑1 (MQ=255)
          gCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTGAGCTAATATCAGTTGTGAATGGTTCAAAATTGTAGCAATTTTGa                                                                                                                                             <  2:1002135/150‑1 (MQ=255)
           ccTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTATTGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGaa                                                                                                                                            <  2:4644102/150‑1 (MQ=255)
            cTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTTTCGGTAATGTTCTTTAGCTAAGATCTGTTGTGGATGGTTCAAAATTGCTACAATTTTGAAc                                                                                                                                           >  2:2890388/1‑150 (MQ=255)
             tACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAAcc                                                                                                                                          >  2:2542647/1‑150 (MQ=255)
                   gTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATGACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAAccattcca                                                                                                                                    <  2:4130028/150‑1 (MQ=255)
                     cAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAAccattccatt                                                                                                                                  <  1:3457421/150‑1 (MQ=255)
                     cAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAAccattccatt                                                                                                                                  <  2:1340391/150‑1 (MQ=255)
                      aaCGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAAACTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCAttt                                                                                                                                 >  1:2879263/1‑150 (MQ=255)
                         gTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGTTTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTc                                                                                                                              <  1:2656292/150‑1 (MQ=255)
                          tATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCa                                                                                                                             <  1:4416305/150‑1 (MQ=255)
                            tctcATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCata                                                                                                                           <  2:1483836/150‑1 (MQ=255)
                            tctcATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCata                                                                                                                           >  2:1891093/1‑150 (MQ=255)
                            tctcATCCCGGCGCTTAAAACCAGGCGTTACATCGGAAAACTGATTAAGCAGATAGTGGGTATACAGTGCGTATCGGTAAAGTTCTTTAGCTAATATAAGTTGTGGATGGTTAAAAAATGCTAAAATTTTTAACCATTACAATTTTAata                                                                                                                           >  1:2322389/1‑150 (MQ=255)
                             ctcATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCataa                                                                                                                          <  1:1457752/150‑1 (MQ=255)
                              tcATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCataat                                                                                                                         >  2:3881532/1‑150 (MQ=255)
                               cATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCataata                                                                                                                        <  1:812885/150‑1 (MQ=255)
                                aTCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAatat                                                                                                                       >  2:393328/1‑150 (MQ=255)
                                   ccGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCa                                                                                                                    <  2:3022525/150‑1 (MQ=255)
                                    cGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCAt                                                                                                                   <  2:1478931/150‑1 (MQ=255)
                                      gcgcTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCAttt                                                                                                                 <  2:673323/150‑1 (MQ=255)
                                         cTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGtt                                                                                                              <  1:4280479/150‑1 (MQ=255)
                                         cTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGtt                                                                                                              <  2:4707605/150‑1 (MQ=255)
                                          ttAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAGCCATTCCATTTTTCATAATATTCATTTGtta                                                                                                             >  1:3096951/1‑150 (MQ=255)
                                                ccTGGCTGTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATTGTTCAAAATTGCTACAATTTTGTACCATTCCATTTTTCATAATATTCATTTGTTATTATTc                                                                                                       <  1:1541685/150‑1 (MQ=255)
                                                ccTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTc                                                                                                       >  1:2244377/1‑150 (MQ=255)
                                                ccTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTc                                                                                                       >  2:857181/1‑150 (MQ=255)
                                                ccTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTc                                                                                                       >  1:4623857/1‑150 (MQ=255)
                                                  tGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCa                                                                                                     >  2:3371103/1‑150 (MQ=255)
                                                  tGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCa                                                                                                     >  2:3492488/1‑150 (MQ=255)
                                                  tGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCa                                                                                                     >  2:3063982/1‑150 (MQ=255)
                                                          cATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCa                                                                                             <  1:136048/150‑1 (MQ=255)
                                                           aTCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCAc                                                                                            <  1:2945470/150‑1 (MQ=255)
                                                            tCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACt                                                                                           <  1:144469/150‑1 (MQ=255)
                                                             cGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACtt                                                                                          <  2:1756916/150‑1 (MQ=255)
                                                                    cTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGt                                                                                   <  1:2513742/150‑1 (MQ=255)
                                                                     tGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTc                                                                                  <  1:861767/150‑1 (MQ=255)
                                                                          aCGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATc                                                                             <  2:2428889/150‑1 (MQ=255)
                                                                            gCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCt                                                                           >  2:3753782/1‑150 (MQ=255)
                                                                             cTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTa                                                                          <  1:3236067/150‑1 (MQ=255)
                                                                             cTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTa                                                                          <  2:4634764/150‑1 (MQ=255)
                                                                             cTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTa                                                                          <  2:2217625/150‑1 (MQ=255)
                                                                               gATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGt                                                                        <  2:2650271/150‑1 (MQ=255)
                                                                                 tCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGtat                                                                      >  2:1226833/1‑150 (MQ=255)
                                                                                       gTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCag                                                                >  1:4048967/1‑150 (MQ=255)
                                                                                        tATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCagt                                                               >  2:2205000/1‑150 (MQ=255)
                                                                                        tATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCagt                                                               <  1:1891093/150‑1 (MQ=255)
                                                                                         aTCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCagtt                                                              >  1:3989458/1‑150 (MQ=255)
                                                                                         aTCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTAGTATCagtt                                                              >  1:2546277/1‑150 (MQ=255)
                                                                                         aTCCTGTGCGTATCGGAAATGTTCTTTAGCTAATATCAGTTGTGGATGCTACAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCagtt                                                              <  2:3469036/150‑1 (MQ=255)
                                                                                            cTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTATCTCGTCATATCCTAGTATTATCagttagt                                                           >  1:4250973/1‑150 (MQ=255)
                                                                                             tgtgCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGCTGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCagttagtt                                                          <  2:3845554/150‑1 (MQ=255)
                                                                                             tgtgCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCagttagtt                                                          >  2:4370264/1‑150 (MQ=255)
                                                                                              gtgCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAgttagtta                                                         <  2:4408078/150‑1 (MQ=255)
                                                                                                gCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTaaa                                                       <  2:1716086/150‑1 (MQ=255)
                                                                                                gCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTaaa                                                       <  1:1572487/150‑1 (MQ=255)
                                                                                                gCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTaaa                                                       >  2:4649818/1‑150 (MQ=255)
                                                                                                  gTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAaca                                                     <  1:3591534/150‑1 (MQ=255)
                                                                                                     tCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAacacac                                                  <  1:2147666/150‑1 (MQ=255)
                                                                                                     tCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAacacac                                                  <  1:3217903/150‑1 (MQ=255)
                                                                                                     tCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAacacac                                                  <  2:961679/150‑1 (MQ=255)
                                                                                                       ggTCATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACaa                                                >  2:517842/1‑150 (MQ=255)
                                                                                                       ggTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACaa                                                >  2:708126/1‑150 (MQ=255)
                                                                                                       ggTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACaa                                                >  1:4169962/1‑150 (MQ=255)
                                                                                                       ggTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTACAAAATTTCTACAATTTTGAACCATTCCATTTTTAATAATAATCATTTGTTATTATTCAACCCTAAAACTAATCTCGTCATATCCTAGTATTATAAGTTAGTTAAACACACaa                                                >  1:717284/1‑150 (MQ=255)
                                                                                                        gTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACaaa                                               >  2:1383256/1‑150 (MQ=255)
                                                                                                         tAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAag                                              >  2:3716475/1‑150 (MQ=255)
                                                                                                             gTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATAAGTTAGTTAAACACACAAAGAGAt                                          >  1:4059147/1‑150 (MQ=255)
                                                                                                                       tAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCt                                <  1:3735075/150‑1 (MQ=255)
                                                                                                                        aagatCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCtt                               <  2:4498870/147‑1 (MQ=255)
                                                                                                                         atatCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTa                              <  1:498688/150‑1 (MQ=255)
                                                                                                                           atCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTaaa                            <  1:2004249/150‑1 (MQ=255)
                                                                                                                           atCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTaaa                            <  1:2447639/150‑1 (MQ=255)
                                                                                                                              aGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATaa                         <  2:1399824/150‑1 (MQ=255)
                                                                                                                                ttGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATAAGa                       <  2:843083/150‑1 (MQ=255)
                                                                                                                                ttGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATAAGa                       <  2:214614/150‑1 (MQ=255)
                                                                                                                                aatggaaTGGTTCAAAATTGTAGCAATTTTGAACCATCCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATAAGa                       >  1:3153461/7‑150 (MQ=39)
                                                                                                                                  gtgGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATAAGACt                     <  1:1821635/150‑1 (MQ=255)
                                                                                                                                      aTGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATAAGACTAttt                 >  2:4297968/1‑150 (MQ=255)
                                                                                                                                       tGGTTCAAAATTGTAGCAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATAAGACTATTTa                >  1:1069895/1‑150 (MQ=255)
                                                                                                                                         gTTCAACATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATAAGACTATTTAAg              <  1:2205872/150‑1 (MQ=255)
                                                                                                                                          ttCAAAATTGCTACTATTTTGAACCATTCCATTTTTCATAAGATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATAAGACTATTTAAGa             <  2:3303261/150‑1 (MQ=255)
                                                                                                                                          ttCAAAATTGCTACACTTATGAACCAGTCCATTTTTCCTCATATTCAGTTGTTATTATTCCACCCTATCACGTCTCTCGTCATCTCCTAGTATTATCAGTTAGTTACACACACAAAGAGATCTGAACATCTTAAATAGTCTTATTTAAGa             <  2:4045014/150‑1 (MQ=255)
                                                                                                                                          ttCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATAAGACTATTTAAGa             >  1:3902646/1‑150 (MQ=255)
                                                                                                                                          ttCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATAAGACTATTTAAGa             <  1:1011898/150‑1 (MQ=255)
                                                                                                                                          ctCAAAATTGCTACAATTTTGATCCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATAAGACTATTTaaaa             <  1:925537/149‑3 (MQ=255)
                                                                                                                                           tCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATAAGACTATTTAAGAt            >  1:4707316/1‑150 (MQ=255)
                                                                                                                                             aaaaTTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATAAGACTATTTAAGATGc          >  2:1111124/1‑150 (MQ=255)
                                                                                                                                                  tGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATAGTCTTATTTAAGATgttcaga     <  2:899463/150‑7 (MQ=255)
                                                                                                                                                   gCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATAAGACTATTTAAGATGCATAACt    <  2:2376106/150‑1 (MQ=255)
                                                                                                                                                     tACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATAAGACTATTTAAGATGCATAACTTa  >  1:681449/1‑150 (MQ=255)
                                                                                                                                                     tACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATAAGACTATTTAAGATGCATAACTTa  >  2:3993148/1‑150 (MQ=255)
                                                                                                                                                     tACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATAAGACTATTTAAGATGCATAACTTa  <  1:393328/150‑1 (MQ=255)
                                                                                                                                                     tACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATAAGACTATTTAAGATGCATAACTTa  >  2:2014956/1‑150 (MQ=255)
                                                                                                                                                     tACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATAAGACTATTTAAGATGAATAACTTa  >  2:2633367/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TTTTCATCATGCCTACGCTGTCAACGTATCTCATCCCGGCGCTTAAACCCTGGCGTTCCATCGGAAATCTGATTACGCTGATCGTGGGTATCCTGTGCGTATCGGTAATGTTCTTTAGCTAATATCAGTTGTGGATGGTTCAAAATTGCTACAATTTTGAACCATTCCATTTTTCATAATATTCATTTGTTATTATTCCACCCTATCACTTCTCTCGTCATATCCTAGTATTATCAGTTAGTTAAACACACAAAGAGATCTGAACATCTTAAATAAGACTATTTAAGATGCATAACTTA  >  NC_000913/2986955‑2987253

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: