Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,270,101 C→A 6.5% intergenic (+499/+115) yhaC → / ← rnpB pentapetide repeats‑related protein/RNase P, M1 RNA component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,270,1010CA 6.5% 788.5 / 12.9 271intergenic (+499/+115)yhaC/rnpBpentapetide repeats‑related protein/RNase P, M1 RNA component
Reads supporting (aligned to +/- strand):  ref base C (127/115);  new base A (18/6);  total (146/125)
Fisher's exact test for biased strand distribution p-value = 5.13e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 279 total aligned reads displayed.

AGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTG  >  NC_000913/3269954‑3270250
                                                                                                                                                   |                                                                                                                                                     
   cacaccccGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCAGTCATCTTTCTGCCCCTCCAACAGCAAAAACCCGCCGAAGCGGGTATTTACGTAAACCAGGt                                                                                                                                                  <  1:2802305/145‑1 (MQ=255)
     aaaCCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGa                                                                                                                                                <  2:504142/150‑1 (MQ=37)
       aCCCGCCGAAGCGGGTTTTTACGTACAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAACAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGaaa                                                                                                                                              <  1:2173513/150‑1 (MQ=255)
       aCCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTGGGGTATAGTGTCGTGGACAGGCCATCAACTTTCAGCCCCTCCAAAAGCAAAAACCCGCCGAAGAGCGTTTGTACGTAAAACAGCAGaaa                                                                                                                                              >  2:3919256/1‑150 (MQ=11)
        cccGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAAc                                                                                                                                             <  1:1579559/150‑1 (MQ=37)
        cccGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCAATCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTGTTTACGTAAACCAGGTGAAAc                                                                                                                                             >  1:4084602/1‑150 (MQ=255)
         ccgccgAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCACCAAAAGCAAAAACCCGCCGAAGCCGGTTTTTACGTAAACCAGGTGAAACt                                                                                                                                            >  2:610490/1‑150 (MQ=255)
         ccgccgAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGAGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACt                                                                                                                                            >  1:204599/1‑150 (MQ=17)
            ccgAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGAc                                                                                                                                         <  1:2263777/150‑1 (MQ=37)
             cgAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGAcc                                                                                                                                        <  2:2955694/150‑1 (MQ=17)
                 gCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATa                                                                                                                                    <  2:4145124/150‑1 (MQ=37)
                   gggTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAg                                                                                                                                  <  1:2460830/150‑1 (MQ=37)
                   gggTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCATTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAg                                                                                                                                  >  2:548702/1‑150 (MQ=21)
                           aCGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGctttc                                                                                                                          >  2:2321480/1‑150 (MQ=40)
                               aaaaCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCtttt                                                                                                                      <  2:2549141/150‑1 (MQ=40)
                                aaaCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTg                                                                                                                     <  2:252286/150‑1 (MQ=39)
                                aaaCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTg                                                                                                                     <  1:4021752/150‑1 (MQ=39)
                                aaaCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTg                                                                                                                     <  1:3694329/150‑1 (MQ=39)
                                  tcAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCCTCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTggg                                                                                                                   <  2:2746069/149‑1 (MQ=18)
                                  aCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTggg                                                                                                                   <  1:1000879/150‑1 (MQ=25)
                                      gttaaaCTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGtata                                                                                                               <  2:372550/147‑1 (MQ=21)
                                      gTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGGCAGTCATTCATCTTTCTGCCCCGCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACAGACCGATAAGCCCCTTTCTGTTGGGtcta                                                                                                               >  1:1018712/1‑150 (MQ=11)
                                      gTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTAATTCATCTTTCTGCCCCTACAAAAGCAAAAACACGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGAACGAAAAGCCGCTTTCTTGTGGGgata                                                                                                               >  2:2877279/1‑150 (MQ=11)
                                       tGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGAGTCGTGGACAGTCAATAAACTTTATGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAAAAGGTGTAACTGACCGATAAGCCGCGTTCTTGTgggggacg                                                                                                              >  1:3821866/1‑145 (MQ=11)
                                           aCTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGcgtc                                                                                                          <  1:2472272/150‑1 (MQ=37)
                                             tGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGa                                                                                                                                          <  1:3226675/116‑1 (MQ=35)
                                             tGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGcgtcgt                                                                                                        <  1:4378589/150‑1 (MQ=37)
                                             tGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACAGACCGATAAGCCGCGTTCTTTTGGGGATAGcgtcgt                                                                                                        >  1:2945900/1‑150 (MQ=255)
                                              gACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTg                                                                                                       <  1:3414225/150‑1 (MQ=37)
                                               aCCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACACTCATTCATCTTTCTGCCCCACCAAAAGCAAAAAACCGCCCAAGCGGGTTTTTACCTAAAACACGTGAAACTGAACGATAAGCCGCGTTCTTTTTGGTATAGCGTCGTgg                                                                                                      >  1:2951105/1‑150 (MQ=11)
                                                ccGATAAGCCGCTTTCTTTTGGGTAGAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCACAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGa                                                                                                     <  2:4495351/150‑1 (MQ=255)
                                                  gATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACa                                                                                                   <  2:3020097/150‑1 (MQ=37)
                                                            tttctttTGGGTATAGTGTCGTGGACAGTCATTCATATTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACAGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGtcaatcaaa                                                                                         >  1:1133897/1‑148 (MQ=255)
                                                            tttctttTGGGTATAGTGTCGTGGACAGTCATTCAACTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCCGGTTTTTACGTAAACCAGGTGAAACAGACCGATAAGCCGCTTTCTTGTGGGTATAGAGTAGTGGACAGTCAatcatc                                                                                         >  2:1043733/1‑150 (MQ=11)
                                                             ttctttTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCAAGTCAATCATCt                                                                                        >  1:2629404/1‑150 (MQ=255)
                                                                ttttGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCAACTTTc                                                                                     >  2:2404519/1‑150 (MQ=37)
                                                                ttttGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCAAATTTc                                                                                     >  2:3468515/1‑150 (MQ=255)
                                                                     ggTTTAGTGTCTTGGACAGTCTTTCTTCTTTCTGCCCCTCCAAACGCAAATACCCGCCGTATCGGGTTTTTACTTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGccc                                                                                <  1:4443010/150‑1 (MQ=11)
                                                                     ggTATAGTGTCGTGGACAGTCATTCATCTTTCAGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTATTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGccc                                                                                <  1:1390855/150‑1 (MQ=255)
                                                                      gTATAGTGTCGTGGACAGTCATTCATATTTATGCCCCACCAAAAGCAAAAAACCCCCGAAGCGGGTTTTTACGTAAACCAGGTGTAAAAGACCGATAAGCCGCTTTCTTTTGGGTATATCGGCGTTGGCAGTCAATCAAAATTATGcccc                                                                               >  1:935079/1‑150 (MQ=11)
                                                                       tataGTGTCGTGGACAGTCATTCATCTTTCTGCCCCACCAAAATCAAAAACCCCCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACCGTCAATCATCATTAAGcccca                                                                              >  1:2827198/1‑149 (MQ=11)
                                                                             gtCGTTGACAGTCATTCATCTTTCAGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCaaaa                                                                        <  1:4427349/150‑1 (MQ=12)
                                                                             gtCGTTGACAGCCTTTCATATTTATGCCCCTCAACAATCAAAAACCCGCCGCAGCTGGTTTTTACGTAAACCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGGGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCaaaa                                                                        <  2:1236142/150‑1 (MQ=11)
                                                                             gtCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCaaaa                                                                        <  1:634374/150‑1 (MQ=37)
                                                                             gtCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCaaaa                                                                        <  2:4616855/150‑1 (MQ=37)
                                                                                  ggACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGTTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCaaa                                                                   <  2:2467143/150‑1 (MQ=37)
                                                                                    aCAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCAGGCAGTCAACATTCAGCCCCACCAAAAGCaaaaa                                                                 >  2:2154502/1‑150 (MQ=11)
                                                                                      aGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAAcc                                                               >  1:1741197/1‑150 (MQ=37)
                                                                                      aGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAAcc                                                               >  2:2200177/1‑150 (MQ=37)
                                                                                         cattcatCTTTCTGCCCCTCCAAAAGCAAACACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTTTAGCGTGGTGGACAGTCATTCTTCTTTCTGCCCCTCCAAAAGCAAAAACccgc                                                            <  1:3844517/150‑1 (MQ=255)
                                                                                         cattcatCTTACTGCCCCTCCAAAAGCAAAACCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACccgc                                                            <  1:4740915/150‑1 (MQ=255)
                                                                                          attcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACccgcc                                                           >  2:3283399/1‑150 (MQ=37)
                                                                                          attcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACccgcc                                                           >  2:2023359/1‑150 (MQ=37)
                                                                                          attcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCGTTCTTTTGGGTATAGCGTCGGGGAAAGTCAAGCAAAATTAAGCGCGGCCAAAAGCTAAAACccgcc                                                           >  1:2373600/1‑150 (MQ=11)
                                                                                           ttcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAAAccgccg                                                          >  2:1759123/1‑150 (MQ=37)
                                                                                            tcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTGGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGa                                                         >  1:4258768/1‑150 (MQ=37)
                                                                                             catCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGGATAGCGTCGTGGACAGTCATTCATCTGTCTGCCCCTCCAAAAGCAAAAACCCGCCGaa                                                        >  2:803177/1‑150 (MQ=255)
                                                                                                cTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCg                                                     <  2:1800147/150‑1 (MQ=37)
                                                                                                 tttCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCAATCATCGTTCTGCCCCACCAAAAGCAAAAACCCGCCGAAGCgg                                                    >  2:3758488/1‑150 (MQ=255)
                                                                                                 tttCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTGTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACCGTCAGTCATCTTGCTGCCCCTCCAAAAGCAAAAACCCGCCGcggcgg                                                    >  1:3092902/1‑150 (MQ=11)
                                                                                                   tCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGt                                                  >  2:1094887/1‑150 (MQ=37)
                                                                                                   tCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCGCCAAAAGCAAAAACCCGCCGAAGCGGGt                                                  >  2:35730/1‑150 (MQ=37)
                                                                                                   tCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATATTTCTGCCCCTCCAAAAGCAAAAACCCGCCGCAGCGGGt                                                  >  2:4092792/1‑150 (MQ=255)
                                                                                                     tGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGttt                                                <  2:2503517/150‑1 (MQ=17)
                                                                                                      gCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCAGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGtttt                                               >  2:748690/1‑150 (MQ=37)
                                                                                                      gCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCGTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGtttt                                               >  2:4386819/1‑150 (MQ=37)
                                                                                                       ccccTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGttttt                                              <  2:1389438/150‑1 (MQ=37)
                                                                                                         ccTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTAc                                            >  2:4452974/1‑150 (MQ=17)
                                                                                                         ccTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTAc                                            >  1:185208/1‑150 (MQ=37)
                                                                                                          cTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACg                                           >  2:347239/1‑150 (MQ=37)
                                                                                                             cAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTaa                                        >  2:46646/1‑150 (MQ=37)
                                                                                                             cAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTaa                                        >  2:3942010/1‑150 (MQ=37)
                                                                                                             cAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTGTTGGGTATAGCGTCGTGGACAGTCCTTCATCTTTCTGCCCCGCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTaa                                        >  2:2132511/1‑150 (MQ=255)
                                                                                                              aaaaGCATAAACCCGCCGCAGCGGGATTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCATACATAAGCGTATACCGACGAGGCCAGTCCATCAACTTTCTGCCCCACCAAAAGCCAAAACCCGCCGCAGCGGGGGATTACGTcaa                                       >  2:3047227/1‑150 (MQ=2)
                                                                                                              aaaaGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCGTTCTGCCCCGCCAAAAGCAAAAACCCGCCGAAGCGGGTGTTGACGTaaa                                       >  1:4685488/1‑150 (MQ=255)
                                                                                                               aaaGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAt                                      >  1:780542/1‑150 (MQ=38)
                                                                                                               aaaGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCGCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAt                                      >  1:641795/1‑150 (MQ=38)
                                                                                                                  gtaaaaaCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACccg                                                             <  1:2509840/122‑1 (MQ=18)
                                                                                                                    aaaaaCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGt                                 >  2:1700986/1‑150 (MQ=37)
                                                                                                                    aaaaaCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAAACCGCCGAAGCGGGGTTTTACGTAAATCAGGt                                 >  1:3526394/1‑150 (MQ=255)
                                                                                                                     aaaaCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCAGTCATTCATCATTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAGCAGGTg                                >  2:3990990/1‑150 (MQ=255)
                                                                                                                     aaaaCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTGTTTACGGAAATCAGGTg                                >  2:3652766/1‑150 (MQ=255)
                                                                                                                      aaaCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGa                               >  1:3905642/1‑150 (MQ=37)
                                                                                                                        aCCCGCCGAAGCGGGTTTTTACGTAAACCCGGTGAAACTGTCCGATAATCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGaaa                             <  1:2440526/150‑1 (MQ=255)
                                                                                                                              cgAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCGCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTAAGTAAATCAGGTGAAACTGAcc                       >  1:4135649/1‑150 (MQ=255)
                                                                                                                                taGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGa                     <  1:4452974/149‑1 (MQ=37)
                                                                                                                                aaGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGa                     <  1:1170080/150‑1 (MQ=37)
                                                                                                                                aaGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGa                     <  2:1836056/150‑1 (MQ=37)
                                                                                                                                      gTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGcc               >  2:1597929/1‑150 (MQ=37)
                                                                                                                                       tttttACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGGCATTCATCTTTCAGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTGTTTACGTAAATCAGGTGAAACTGACCGATAAGCCg              >  1:3472584/1‑150 (MQ=255)
                                                                                                                                       tttttACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAATCCg              <  2:3532485/150‑1 (MQ=12)
                                                                                                                                        ccttACGTACCCCAGGTGAAACTGACCGATAAGCCGCCTTCTTTTGGTTATAGGGTCGTGGACTGTGATTCATCTTACTGCCCCTCCACAACCAAAAACCCGCCGAATCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCgg             <  1:945915/148‑1 (MQ=11)
                                                                                                                                           tACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGtt          >  1:3307302/1‑150 (MQ=40)
                                                                                                                                              gTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTg       >  1:41338/1‑150 (MQ=255)
                                                                                                                                              gTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTg       >  1:4604447/1‑150 (MQ=17)
                                                                                                                                                aaaCCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAAAAGGTGAAACAGACCGATAAGCCGGGTTCTGTc     >  2:2223516/1‑150 (MQ=255)
                                                                                                                                                aaaCCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAAACCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGTCCGATAAGCCGGGTTCTGtg     >  2:3414481/1‑149 (MQ=255)
                                                                                                                                                  aCCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGt   <  2:4408485/150‑1 (MQ=255)
                                                                                                                                                   ccAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTg  >  2:1311866/1‑150 (MQ=255)
                                                                                                                                                   ccAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCCCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGGTCTGTCGTg  >  1:452304/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
AGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTG  >  NC_000913/3269954‑3270250

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: