Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,799,370 Δ1 bp 6.0% intergenic (‑100/+23) pheM ← / ← rplT phenylalanyl‑tRNA synthetase operon leader peptide/50S ribosomal subunit protein L20

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,799,3700A. 6.0% 448.1 / 21.5 149intergenic (‑100/+23)pheM/rplTphenylalanyl‑tRNA synthetase operon leader peptide/50S ribosomal subunit protein L20
Reads supporting (aligned to +/- strand):  ref base A (74/63);  new base . (3/6);  total (79/70)
Fisher's exact test for biased strand distribution p-value = 3.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.75e-01

Only 100 of 151 total aligned reads displayed.

ATTCAGGTGCTAAAGTAAAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCAACAGAGGCTTTTTTCAGGCCATTGATGAATTT  >  NC_000913/1799224‑1799518
                                                                                                                                                  |                                                                                                                                                     
aTTCATGTGCTAAAGTAAAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTa                                                                                                                                                    <  1:1287583/150‑1 (MQ=255)
   cAGGTGCTAAAGTAAAAAAAGAAGCGGAAAATAGCAGCATTCAATGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCt                                                                                                                                                <  1:1565904/150‑1 (MQ=255)
        gCTAAAGTAAAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCctc                                                                                                                                           <  1:1232359/150‑1 (MQ=255)
        gCTAAAGTAAAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATAGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCctc                                                                                                                                           >  2:1108614/1‑150 (MQ=255)
          tAAAGTAAAAAAAGAAGCGTAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGCTAGTCTCCCTCtt                                                                                                                                         <  2:1698230/150‑1 (MQ=255)
          tAAAGTAAAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCtt                                                                                                                                         >  1:1821921/1‑150 (MQ=255)
           aaaGTAAAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCttt                                                                                                                                        <  2:1712995/150‑1 (MQ=255)
           aaaGTAAAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCttt                                                                                                                                        >  1:967367/1‑150 (MQ=255)
            aaGTAAAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTc                                                                                                                                       <  1:180647/150‑1 (MQ=255)
            aaGTAAAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGCTAGTCTCCCTCtttt                                                                                                                                       <  1:1789452/150‑2 (MQ=255)
            aaGTAAAAAAACAAGCGGAAAATAGCACCATTCATTGCATGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTc                                                                                                                                       <  1:149434/150‑1 (MQ=255)
             aGTAAAAAAAGAAGCGGAAATTAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCa                                                                                                                                      <  1:281544/150‑1 (MQ=255)
             aGTAAAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCa                                                                                                                                      <  2:190469/150‑1 (MQ=255)
             aGTAAAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCa                                                                                                                                      >  2:612482/1‑150 (MQ=255)
              gTAAAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCaa                                                                                                                                     >  2:1809743/1‑150 (MQ=255)
                aaataaaGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTAGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGACAATGTTTTGATTGCGTTGAAACGAAAATATGGAGACTAGCTCCCTCTTTCAACt                                                                                                                                   >  2:259097/1‑150 (MQ=255)
                aaaaaaaGAAGCTGAAAATAGAAGCATTCATTGCTTGGGTTAGCTTTTGGTACTCTTCAATAGACCTTTTTTGAAAAGGCTACGGCGATAAAATTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACt                                                                                                                                   <  2:199326/150‑1 (MQ=255)
                aaaaaaaGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACt                                                                                                                                   >  1:229925/1‑150 (MQ=255)
                aaaaaaaGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTGTCAACt                                                                                                                                   >  1:1716816/1‑150 (MQ=255)
                  aaaaaGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTgg                                                                                                                                 >  1:1597259/1‑150 (MQ=255)
                    aaaGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCt                                                                                                                               <  1:514718/150‑1 (MQ=255)
                      agaagCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTa                                                                                                                             >  2:1267222/1‑150 (MQ=255)
                         agCGTAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGc                                                                                                                          <  2:920476/150‑1 (MQ=255)
                          ccGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGcc                                                                                                                         <  1:99292/149‑1 (MQ=255)
                            ggAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCag                                                                                                                       <  2:1672736/150‑1 (MQ=255)
                                   agcagcATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAgctgct                                                                                                                >  2:1663208/1‑150 (MQ=255)
                                         attcattGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCt                                                                                                          <  1:1728608/150‑1 (MQ=255)
                                          ttcattGCTTGCGTTAACTTTTGGTACTCTTCAAAAGACCTTTATTTAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCTTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCtt                                                                                                         <  2:1580226/150‑1 (MQ=255)
                                             attGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCttttt                                                                                                      >  2:1485309/1‑150 (MQ=255)
                                               tgcttgcGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCa                                                                                                    <  2:1361538/150‑1 (MQ=255)
                                                gcttgcGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCaa                                                                                                   >  1:145026/1‑150 (MQ=255)
                                                 cttgcGTTACCTTTTGTTACTCTTCAAAAGAACTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAAc                                                                                                  <  1:1322653/150‑1 (MQ=255)
                                                  ttgcGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAAcc                                                                                                 <  2:355058/150‑1 (MQ=255)
                                                   tgcGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGTCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCa                                                                                                <  2:1420136/150‑1 (MQ=255)
                                                   tgcGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGGTGAAACGAAAAGAGGGAGCGTAGCTCCCTCTTTCAACTGGCTGATGCCAGAGCTGCTTTGGGGTTTTCATCCa                                                                                                >  2:959332/1‑150 (MQ=255)
                                                    gcGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCag                                                                                               <  2:1113602/150‑1 (MQ=255)
                                                      gTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCagag                                                                                             <  1:1102321/150‑1 (MQ=255)
                                                          ccTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGt                                                                                         <  1:1634546/150‑1 (MQ=255)
                                                           cTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTg                                                                                        <  2:495754/150‑1 (MQ=255)
                                                              ttGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAACAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAAc                                                                                     <  1:1856251/150‑1 (MQ=255)
                                                                ggTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGc                                                                                   >  1:854984/1‑150 (MQ=255)
                                                                   aCTCTTCAAAAGACCTTTATTGAAAAGGCTACGTCGATAAAAGTCAATGTTTTGCTGGCGTTGAAACTAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTAc                                                                                <  2:104016/150‑1 (MQ=255)
                                                                      ctTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGACCGCTACttt                                                                             >  2:1833585/1‑150 (MQ=255)
                                                                      ctTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACttt                                                                             >  1:418052/1‑150 (MQ=255)
                                                                      ctTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACttt                                                                             >  1:1371895/1‑150 (MQ=255)
                                                                      ctTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACttt                                                                             >  1:230205/1‑150 (MQ=255)
                                                                        tCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGt                                                                           >  2:218719/1‑150 (MQ=255)
                                                                        tCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCATTTTCAACCAGAGCGGTGAACGCTACTTTGt                                                                           >  1:1959651/1‑150 (MQ=255)
                                                                          aataGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGTAGTTGAAAAGAAAAGTGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCg                                                                         <  2:950753/147‑1 (MQ=255)
                                                                                ccTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACt                                                                   >  2:1537957/1‑150 (MQ=255)
                                                                                ccTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGATGCTTTCGCTTTTTCACCCAGAGCGGTGAACGCTACTTTGTCGAATACt                                                                   >  2:66731/1‑150 (MQ=255)
                                                                                 cTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTCTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTg                                                                  <  1:606547/150‑1 (MQ=255)
                                                                                  tttATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGc                                                                 >  1:401059/1‑150 (MQ=255)
                                                                                     aTTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAATGAGGGAGACTAGCTCCCTCTTTCAACTGGCGTATGCCAGAGCTGCTTTCGCTTTTTCAAACAGAGCGGTGATCGCTACTTTGTCGAATACTGAGat                                                              >  2:248181/1‑150 (MQ=255)
                                                                                     aTTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGat                                                              <  1:529651/150‑1 (MQ=255)
                                                                                      ttGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGata                                                             >  1:1289705/1‑150 (MQ=255)
                                                                                        gAAAAGGCTACGGCGATAAAAGTCATTGTTTTGATCGCGTTGAAACGAAAAGAGAGAGACAAGCTCCCGCATACAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAAACAGAGCGGTCAACGCTACTTTGTCGAATACAGCGATATc                                                           >  1:49598/1‑150 (MQ=255)
                                                                                        gAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCGCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATc                                                           >  1:813120/1‑150 (MQ=255)
                                                                                        gAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGTGGTGAACGCTACTTTGTCGAATACTGCGATATc                                                           >  1:1663433/1‑150 (MQ=255)
                                                                                          aaaGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAg                                                         <  2:407031/150‑1 (MQ=255)
                                                                                                  cGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCCGCCAGGATc                                                 >  2:949361/1‑150 (MQ=255)
                                                                                                   ggCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCt                                                >  2:255893/1‑150 (MQ=255)
                                                                                                   ggCGATAAAAGTCAATGTTTTGATGGAGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCt                                                <  1:745033/150‑1 (MQ=255)
                                                                                                    gCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGTCTTATGCCAGAGCTGCTTTCGCTTTTTCACCCAGAGCGGTGAACGCTACTTTGTCGAATACTGGGATATCAGCCAGGATCtt                                               >  2:127331/1‑150 (MQ=255)
                                                                                                    gCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGGTCtt                                               >  1:362435/1‑150 (MQ=255)
                                                                                                      gATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTAc                                             <  2:1371895/150‑1 (MQ=255)
                                                                                                      gATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTAc                                             <  2:1597259/150‑1 (MQ=255)
                                                                                                          aaaGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTc                                         <  1:218719/150‑1 (MQ=255)
                                                                                                            aGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTcta                                       >  1:1096351/1‑148 (MQ=255)
                                                                                                             gTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGAt                                      <  1:486635/150‑1 (MQ=255)
                                                                                                               cAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGAttt                                    <  2:777959/150‑1 (MQ=255)
                                                                                                               cAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGAttt                                    >  1:1234752/1‑150 (MQ=255)
                                                                                                                aaTGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGGTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTc                                   <  1:84359/150‑1 (MQ=255)
                                                                                                                aaTGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTc                                   <  2:814378/150‑1 (MQ=255)
                                                                                                                aaTGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTc                                   <  2:1887842/150‑1 (MQ=255)
                                                                                                                aaTGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTc                                   <  2:329123/150‑1 (MQ=255)
                                                                                                                 aTGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCa                                  <  1:1279712/150‑1 (MQ=255)
                                                                                                                 aTGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGAGATCAGCCAGGATCTTACGGTCGATTTCa                                  >  1:975902/1‑150 (MQ=255)
                                                                                                                  tGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCaa                                 <  1:559780/150‑1 (MQ=255)
                                                                                                                  tGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCaa                                 >  1:739708/1‑150 (MQ=255)
                                                                                                                   gTTTTAATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCAAc                                >  2:1398396/1‑150 (MQ=255)
                                                                                                                      ttGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCAACaga                             <  2:761549/150‑1 (MQ=255)
                                                                                                                        gATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCAACAGAgg                           <  1:1267222/150‑1 (MQ=255)
                                                                                                                            gCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCAACAGAGGCttt                       >  1:1339698/1‑150 (MQ=255)
                                                                                                                             cGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTGATGCCAGAGCTGCTTTCGCTTTTTCAACCATAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGACAGGATCTTACTGTCGATTTCAACAGAGGCtttt                      >  2:813147/1‑150 (MQ=255)
                                                                                                                              gTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCAACAGAGGCttttt                     <  1:532711/150‑1 (MQ=255)
                                                                                                                                tGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCAACAGAGGCTTTTTTc                   >  1:1111119/1‑150 (MQ=255)
                                                                                                                                   aaCGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGGTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGGCGATTTCAACAGAGTCTTTTTTCAgg                >  2:1057932/1‑150 (MQ=255)
                                                                                                                                   aaCGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCAACAGAGGCTTTTTTCAgg                >  2:921566/1‑150 (MQ=255)
                                                                                                                                   aaCGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCAACAGAGGCTTTTTTCAgg                <  1:516500/150‑1 (MQ=255)
                                                                                                                                   aaCGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCAACAGAGGCTTTTTCAGGc               >  1:372720/1‑150 (MQ=255)
                                                                                                                                   aaCGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGAGATATCAGCCAGGATCTTACGGTCGATTTCAACAGAGGCTTTTTTCAgg                >  2:1280970/1‑150 (MQ=255)
                                                                                                                                    aCGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCAACAGAGGCTTTTTTCAGGc               <  1:1962672/150‑1 (MQ=255)
                                                                                                                                     cGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCAACAGAGGCTTTTTTCAGGcc              >  1:1916979/1‑150 (MQ=255)
                                                                                                                                      gAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCAACAGAGGCTTTTTTCAGGCCa             >  1:1174773/1‑150 (MQ=255)
                                                                                                                                      gAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCAACAGAGGCTTTTTTCAGGCCa             >  1:260447/1‑150 (MQ=255)
                                                                                                                                      gAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCGTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCAACAGAGGCTTTTTTCAGGCCa             >  2:29530/1‑150 (MQ=255)
                                                                                                                                       gaaaGAGGGAGCTAGTCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCAACAGAGGCTTTTTTCAGGCCAt            >  1:1400074/2‑150 (MQ=255)
                                                                                                                                             gggAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCAACAGAGGCTTTTTTCAGGCCATtgatga      >  2:1284597/1‑150 (MQ=255)
                                                                                                                                                 gaCTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCAACAGAGGCTTTTTTCAGGCCATTGATGAAttt  >  1:942636/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
ATTCAGGTGCTAAAGTAAAAAAAGAAGCGGAAAATAGCAGCATTCATTGCTTGCGTTACCTTTTGGTACTCTTCAAAAGACCTTTATTGAAAAGGCTACGGCGATAAAAGTCAATGTTTTGATGGCGTTGAAACGAAAAGAGGGAGACTAGCTCCCTCTTTCAACTGGCTTATGCCAGAGCTGCTTTCGCTTTTTCAACCAGAGCGGTGAACGCTACTTTGTCGAATACTGCGATATCAGCCAGGATCTTACGGTCGATTTCAACAGAGGCTTTTTTCAGGCCATTGATGAATTT  >  NC_000913/1799224‑1799518

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: