Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 175,144 Δ1 bp 6.9% coding (38/1422 nt) clcA → H(+)/Cl(‑) exchange transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913175,1440C. 6.9% 392.2 / 23.5 131coding (38/1422 nt)clcAH(+)/Cl(‑) exchange transporter
Reads supporting (aligned to +/- strand):  ref base C (58/63);  new base . (5/4);  total (63/68)
Fisher's exact test for biased strand distribution p-value = 7.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.98e-02

Only 100 of 133 total aligned reads displayed.

GTATAAAAGTTTTGTGCATTTGAAACATTACGCTTTGCAAAGGATTTTCATGGAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCACGCTTGTTGGGCTGGCAGCGGTTGCTTTTGACAAAGGTGTCGCCTGGTTGCAGAACC  >  NC_000913/174999‑175293
                                                                                                                                                 |                                                                                                                                                      
gTATAAAAGTTTTGTGCATTTGAAACATTACGCTTTGCAAAGGATTTTCATGGAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCcgcc                                                                                                                                                    >  2:723120/1‑149 (MQ=255)
gTATAAAAGTTTTGTGCATTTGAAACATTACGCTTTGCAAAGGATTATCATGGAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCcgcg                                                                                                                                                    <  1:255439/150‑1 (MQ=255)
 tataAAAGTTTTGTGCATTTGAAACATTACGCTTTGCAACGGATTTTCATGGAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCcgcgc                                                                                                                                                   <  2:657060/150‑1 (MQ=255)
   taAAAGTTTTGTGCATTTGAAACATTACGCTTTGCAAAGGATTTTCATGGAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCgcgcgc                                                                                                                                                >  2:412768/1‑150 (MQ=255)
    aaaaGTTTTGTGCATTTAAAACATTACGCTTTGCAAAGGATTTTCATGGAAAGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACGAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGcc                                                                                                                                               <  2:545177/150‑1 (MQ=255)
     aaaGTTTTGTGCATTTGAAACATTACGCTTTGCAAAGGATTTTCATGGAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCt                                                                                                                                              >  2:1165179/1‑150 (MQ=255)
       aGTTTTGTGCATTTGAAACATTACGCGTTGCAAAGGATTTTCATGGAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGc                                                                                                                                            <  2:1533943/150‑1 (MQ=255)
        gTTTTGTGCATTTGAAACATTACGCTTTGCAAAGGATTTTCATGGAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCg                                                                                                                                           >  1:1282399/1‑150 (MQ=255)
         ttttGTGCATTTGAAACAATACGCTTTGCAAAGGATTTTCATGGAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCGCGCGGCCTGCgg                                                                                                                                          <  2:1510468/150‑2 (MQ=255)
          tttGTGCATTTGAAACATTACGCTTTGCAAAGGATTTTCATGGAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGAc                                                                                                                                         <  1:703264/150‑1 (MQ=255)
          tttGTGCATTTGAAACATTACGCTTTGCAAAGGATTTTCATGGAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCGCGCGGCCTGCggt                                                                                                                                         <  2:956576/150‑3 (MQ=255)
           ttGTGCATTTGAAACATTACGCTTTGCAAAGGATTTTCATGGAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCGCGCGGCCTGCggtg                                                                                                                                        <  2:982275/150‑4 (MQ=255)
             gtgCATTTGAAACATTACGCTTTGCAAAGGATTTTCATGGAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCATGCCGCGCGCCTGCGACGCa                                                                                                                                      >  1:349259/1‑150 (MQ=255)
             gtgCATTTGAAACATTACGCTTTGCAAAGGATTTTCATGGAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCa                                                                                                                                      <  2:1282399/150‑1 (MQ=255)
             gtgCATTTGAAACATTACGCTTGGCAAAGGATTATCATGGAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCa                                                                                                                                      <  2:470506/150‑1 (MQ=255)
              tgaaTTTGAAACATTACGCTTTGCAAAGGATTTTCATGGAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAg                                                                                                                                     <  2:741913/147‑1 (MQ=255)
              tgCATTTGAAACATTACGCTTTGCAAAGGATTTTCATGGAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGTAg                                                                                                                                     >  1:1711512/1‑150 (MQ=255)
              tgCATTTGAAACATTACGCTTTGCAAAGGATTTTCATGGAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAg                                                                                                                                     >  2:853817/1‑150 (MQ=255)
                cATTTGAAACATTACGCTTTGCAAAGGATTTTCATGGAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGAc                                                                                                                                   >  1:1847754/1‑150 (MQ=255)
                cATTTGAAACATTACGCTTTGCAAAGGATTTTCATGGAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGAc                                                                                                                                   <  1:855647/150‑1 (MQ=255)
                cATTTGAAACATTACGCTTTGCAAAGGATTTTCATGGAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGAc                                                                                                                                   >  1:1301329/1‑150 (MQ=255)
                cATTTGAAACATTACGCTTTGCAAAGGATTTTCATGGAACGTGCGAGTAAAATGCCGTCATATTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTGTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGAc                                                                                                                                   <  2:1581405/150‑1 (MQ=255)
                    tGAAACATTACGCTTTGCAAAGGATTTTCATGGAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACt                                                                                                                               <  2:1847754/150‑1 (MQ=255)
                     gAAACATTACGCTTTGCAAAGGATTTTCATGGAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCTCGCCTGCGACGCAGACAACTg                                                                                                                              >  1:1917767/1‑150 (MQ=255)
                     gAAACATTACGCTTTGCAAAGGATTTTCATGGAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTg                                                                                                                              >  1:1527297/1‑150 (MQ=255)
                        aCATTACGCTTTGCAAAGGATTTTCATGGAACGTGCGAGTAAAATGCCGGCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGAtt                                                                                                                           <  2:1464444/150‑1 (MQ=255)
                         cATTACGCTTTGCAAAGGATTTTCATGGAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCTACGCAGACAACTGATTc                                                                                                                          >  1:1233704/1‑150 (MQ=255)
                           ttACGCTTTGCAAAGGATTTTCATGGAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAATACTGCTACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCTACGCAGACAACTGATTCGc                                                                                                                        >  1:133937/1‑150 (MQ=255)
                                  ttGCAAAGGATTTTCATGGAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAActtc                                                                                                                 >  1:1631140/1‑150 (MQ=255)
                                   tGCAAAGGATTTTCATGGAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGACACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAActtct                                                                                                                >  2:907059/1‑150 (MQ=255)
                                        aGGATTTTCATGGAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAgc                                                                                                           <  2:498182/150‑1 (MQ=255)
                                         ggATTTTCATGGAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCGTTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAgcg                                                                                                          >  2:764880/1‑150 (MQ=255)
                                         ggATTTTCATGGAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAgcg                                                                                                          >  2:390023/1‑150 (MQ=255)
                                         ggATTTTCATGGAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAgcg                                                                                                          <  2:1271202/150‑1 (MQ=255)
                                             tttCATGGAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGAt                                                                                                      <  1:897681/150‑1 (MQ=255)
                                              ttCATGGAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATa                                                                                                     <  2:1538505/150‑1 (MQ=255)
                                                 aTGGAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACACCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTAGCCAACTTCTTGAGCGCGATaaaa                                                                                                  >  1:1769787/1‑150 (MQ=255)
                                                  tGGAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAAc                                                                                                 <  1:1881256/150‑1 (MQ=255)
                                                     aaCGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAAcccc                                                                                              >  1:501569/1‑150 (MQ=255)
                                                        gTGCGAGTAAAATGCCGTCATCTTAGTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGtt                                                                                           <  1:1014853/150‑1 (MQ=255)
                                                          gCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAg                                                                                         <  2:1112361/150‑1 (MQ=255)
                                                          gCGAGTAAAATGCCGTCATATTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAg                                                                                         <  1:1680848/150‑1 (MQ=255)
                                                           cGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACAGCAGGCCGCGCGCCTGCGACCCAGACAACTTATTCGCCAACATCATGAGCGAGATAACACCCCGATatc                                                                                        >  1:338750/1‑148 (MQ=255)
                                                                 aaaTGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCAtttt                                                                                  <  2:1213569/150‑1 (MQ=255)
                                                                  aaTGCTGTCATCTTATTTGTATGACCAATAAGTGAGCATTGGATGAAAACTGATACTCCCTCTTTATAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTg                                                                                 <  1:963608/150‑1 (MQ=255)
                                                                    tGCCGTCTTCTTATTTTTATGAGAAATAAGTTATCATTGGTTTATATCTGTTACTCCCTCTTTAGATACACCGCAGGACGCTCGCCTGGGACGCAGACAACTGATTCGCGAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGtt                                                                               <  1:1536680/150‑1 (MQ=255)
                                                                    tGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGtt                                                                               <  1:195661/150‑1 (MQ=255)
                                                                    tGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGtt                                                                               <  1:102894/150‑1 (MQ=255)
                                                                     gCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGttt                                                                              <  2:205859/150‑1 (MQ=255)
                                                                     gCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACACCGTTAGACATTTTGttt                                                                              >  2:1363424/1‑150 (MQ=255)
                                                                        gTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTTCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATg                                                                           <  1:539169/150‑1 (MQ=255)
                                                                        gTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTCAGAAACACCGCAGGCCGCGCGCCTGAGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATg                                                                           <  2:1193485/150‑1 (MQ=255)
                                                                        gTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAAATGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAAATGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATg                                                                           <  2:1204379/150‑1 (MQ=255)
                                                                         tCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATgg                                                                          <  1:1162959/150‑1 (MQ=255)
                                                                          cATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATggc                                                                         >  2:1042562/1‑150 (MQ=255)
                                                                          cATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATggc                                                                         <  2:1426788/150‑1 (MQ=255)
                                                                           gtCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTTGCCATTTTGTTTATggcg                                                                        >  1:488989/2‑150 (MQ=255)
                                                                             cTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATggcggc                                                                      <  1:1100/150‑1 (MQ=255)
                                                                             cTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATggcggc                                                                      >  2:1685255/1‑150 (MQ=255)
                                                                                  ttGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTCGCCAGTTTGTTTATGGCGGCAgtcg                                                                 >  1:29219/1‑150 (MQ=255)
                                                                                   tGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAgtcgt                                                                <  1:1935013/150‑1 (MQ=255)
                                                                                      aTGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCgg                                                             <  1:1480055/150‑1 (MQ=255)
                                                                                        gACCAATAAGTGATCATTGGATGAAAGCTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCa                                                           >  2:1590317/1‑150 (MQ=255)
                                                                                          ccacttaGTGATATTTGGATGAAAAGTGATACTCCCTCATTAGAAATACCGAAGGCCGCGGGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCACg                                                         <  2:1471043/144‑1 (MQ=255)
                                                                                           cAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCACGc                                                        >  2:1195696/1‑150 (MQ=255)
                                                                                           cAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCACGc                                                        >  2:904117/1‑150 (MQ=255)
                                                                                               aaGTGATCATTGGATGAAAACTGATTCTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCACGCttgt                                                    >  2:1636793/1‑150 (MQ=255)
                                                                                               aaGTGATCAGTGGATGAAAACTGATTCTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGCTTCGCCATCTTCTTGAGCGCGATAAACCCGCGTTAGCCTTTTTGTTTATGGCGGCTGTCGTCGGCACGGttgg                                                    >  2:411320/1‑149 (MQ=255)
                                                                                                 gTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCACGCttgttg                                                  >  2:675753/1‑150 (MQ=255)
                                                                                                      cATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCACGCTTGTTGGGCTg                                             <  1:1110304/150‑1 (MQ=255)
                                                                                                       aTTGGATGAAAAATGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCACGCTTGTTGGGCTgg                                            <  2:133937/150‑1 (MQ=255)
                                                                                                           gATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATATAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCACGCTTGTTGGGCTGGCAGc                                        >  1:1732797/1‑150 (MQ=255)
                                                                                                              gAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCAGTTTGTTTATGGCGGCAGTCGTCGGCACGCTTGTTGGGCTGGCAGCGGt                                     >  2:1830600/1‑150 (MQ=255)
                                                                                                                 aaCTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCACGCTTGTTGGGCTGGCAGCGGTTGc                                  <  2:691038/150‑1 (MQ=255)
                                                                                                                 aaCTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCACGCTTGTTGGGCTGGCAGCGGTTGc                                  <  1:898776/150‑1 (MQ=255)
                                                                                                                    tGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCACGCTTGTTGGGCTGGCAGCGGTTGCttt                               <  2:1872684/150‑1 (MQ=255)
                                                                                                                     gATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCACGCTTGTTGGGCTGGCAGCGGTTGCtttt                              <  1:1673096/150‑1 (MQ=255)
                                                                                                                        aCTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCACGCTTGTTGGGCTGGCAGCGGTTGCTTTTGAc                           <  2:843469/150‑1 (MQ=255)
                                                                                                                            ccTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCACGCTTGTTGGGCTGGCAGCGGTTGCTTTTGACAAAg                       <  2:1887680/150‑1 (MQ=255)
                                                                                                                             ctctTTCGAAACACCGCAGGCCGCGCGCCTGCGACGCACACAACTGATTCGCCAACTTCTTGAGCGCGTTATAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCACGCTTGTTGGGCTGGCAGCGGTTGCTTTTGAGAAAgg                      <  2:949510/150‑1 (MQ=255)
                                                                                                                             ctctTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCGATTTTGTTTATGGCGGCAGTCGTCGGCGCGCTTGTTCGGCAGGCAGCGGTTGCTTTTGACTAAgg                      >  2:798551/1‑150 (MQ=255)
                                                                                                                                tttAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCACGCTTGTTGGGCTGGCAGCGGTTGCTTTTGACAAAGgtgt                   >  1:1610929/1‑150 (MQ=255)
                                                                                                                                  tAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCCCGCTTGTTGGGCTGGCAGCGGTTGCTTTTGACAAAGGTGTCg                 >  1:30470/1‑150 (MQ=255)
                                                                                                                                   aGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCACGCTTGTTGGGCTGGCAGCGGTTGCTTTTGACAAAGGTGTCGc                <  1:466693/150‑1 (MQ=255)
                                                                                                                                    tctgcgtcGCAGGCGCGCGGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCACGCTTGTTGGGCAGGCAGCGGTTGCTTTTGACAAAGGTGTCGcc               >  2:1739444/8‑150 (MQ=255)
                                                                                                                                    gAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCACGCTTGTTGGGCTGGCAGCGGTTGCTTTTGACAAAGGTGTCGcc               >  1:1585979/1‑150 (MQ=255)
                                                                                                                                    gAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCACGCTTGTTGGGCTGGCAGCGGTTGCTTTTGACAAAGGTGTCGcc               >  2:797681/1‑150 (MQ=255)
                                                                                                                                      tgcgtcGCAGGCGCGCGGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCACGCTTGTTGGGCTGGCAGCGGTTGCTTTTGACAAAGGTGTCGCCTg             >  1:1365817/6‑150 (MQ=255)
                                                                                                                                      aaCACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCACGCTTGTTGGGCTGGCAGCGGTTGCTTTTGACAAAGGTGTCGCCTg             <  2:1492751/150‑1 (MQ=255)
                                                                                                                                       acacCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCACGCTTGTTGGGCTGGCAGCGGTTGCTTTTGACGAAGGTGTCGCCTgg            <  2:1527297/150‑1 (MQ=255)
                                                                                                                                       acacCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCACGCTTGTTGGGCTGGCAGCGGTTGCTTTTGACAAAGGTGTCGCCTgg            <  2:501569/150‑1 (MQ=255)
                                                                                                                                        cgtcGCAGGCGCGCGGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCACGCTTGTTGGGCTGGCAGCGGTTGCGTTTGACAAAGGTGTCGCCTGGt           >  1:1668161/4‑150 (MQ=255)
                                                                                                                                         gtcGCAGGCGCGCGGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCACGCTTGTTGGGCTGGCAGCGGTTGCTTTTGACAAAGGTGTCGCCTGGtt          >  2:578819/3‑150 (MQ=255)
                                                                                                                                          ccGCAGGCCGCGCGCCTGCGACGCAGACGACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCACGCTTGTTGGGCTGGCAGCGGTTGCTTTTGACAAAGGTGTCGCCTGGTTg         >  1:373976/1‑150 (MQ=255)
                                                                                                                                           cGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCACGCTTGTTGGGCTGGCAGCGGTTGCTTTTGACAAAGGTGTCGCCTGGTTGc        <  2:540924/150‑1 (MQ=255)
                                                                                                                                            gCAGGCCGCGCGCCTGCGACGCAGACAACTTATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCACGCTTGTTGGGCTGGCAGCGGTTGCTTTTGACAAAGGTGTCGCCTGGTTGCa       <  2:1913083/150‑1 (MQ=255)
                                                                                                                                               ggCCGCGAGCCTGCGACGCCGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTCTGGCGGCAGTCGTCGGCACGCTTGTTGGGCTGGCAGCGGTTGCTTTTGACAAAGGTGTCGCCTGGTTGCAGaa    <  2:1997679/150‑1 (MQ=255)
                                                                                                                                                gCCGCGCGCCTGCGACGCAGACAACTGATTCGCCACCTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCCCGCTTGTTGGGCTGGCAGCGGTTGCTTTTGACAAAGGTGTCGCCTGGTTGCAGAAc   >  1:1240923/1‑150 (MQ=255)
                                                                                                                                                 ccGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCACGCTTGTTGGGCTGGCAGCGGTTGCTTTTGACTAAGGTGTCGCCTGGTTGCAGCAcc  >  2:362085/1‑150 (MQ=255)
                                                                                                                                                 ccGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCACGCTTGTTGGGCTGGCAGCGGTTGCTTTTGACAAAGGTGTCGCCTGGTTGCAGAAcc  >  2:330973/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                                      
GTATAAAAGTTTTGTGCATTTGAAACATTACGCTTTGCAAAGGATTTTCATGGAACGTGCGAGTAAAATGCCGTCATCTTATTTGTATGACCAATAAGTGATCATTGGATGAAAACTGATACTCCCTCTTTAGAAACACCGCAGGCCGCGCGCCTGCGACGCAGACAACTGATTCGCCAACTTCTTGAGCGCGATAAAACCCCGTTAGCCATTTTGTTTATGGCGGCAGTCGTCGGCACGCTTGTTGGGCTGGCAGCGGTTGCTTTTGACAAAGGTGTCGCCTGGTTGCAGAACC  >  NC_000913/174999‑175293

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: